Importance of heat-labile enterotoxin in colonization of the adult mouse small intestine by human enterotoxigenic Escherichia coli strains. Infect Immun 2006 Feb;74(2):869-75
Date
01/24/2006Pubmed ID
16428729Pubmed Central ID
PMC1360293DOI
10.1128/IAI.74.2.869-875.2006Scopus ID
2-s2.0-31844453571 (requires institutional sign-in at Scopus site) 108 CitationsAbstract
Enterotoxigenic Escherichia coli (ETEC) infections are a significant cause of diarrheal disease and infant mortality in developing countries. Studies of ETEC pathogenesis relevant to vaccine development have been greatly hampered by the lack of a suitable small-animal model of infection with human ETEC strains. Here, we demonstrate that adult immunocompetent outbred mice can be effectively colonized with the prototypical human ETEC H10407 strain (colonization factor antigen I; heat-labile and heat-stable enterotoxin positive) and that production of heat-labile holotoxin provides a significant advantage in colonization of the small intestine in this model.
Author List
Allen KP, Randolph MM, Fleckenstein JMAuthor
Kenneth Paul Allen DVM Associate Professor in the Research Office department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBacterial Toxins
Child, Preschool
Colony Count, Microbial
Disease Models, Animal
Enterotoxins
Escherichia coli
Escherichia coli Infections
Escherichia coli Proteins
Female
Humans
Intestine, Small
Mice
Mice, Inbred ICR
Microscopy, Electron, Scanning