Faculty Collaboration Database

Latex allergen IgE assays in the assessment of Veterans Affairs health care workers. Ann Allergy Asthma Immunol 2006 Jun;96(6):840-3

Date

06/29/2006

Pubmed ID

16802772

DOI

10.1016/S1081-1206(10)61347-6

Scopus ID

2-s2.0-33745219904 (requires institutional sign-in at Scopus site)   2 Citations

Abstract

BACKGROUND: A previous multicenter study of Veterans Affairs health care workers evaluated hospital participants for latex hypersensitivity. Well-defined groups from that study allowed us to explore the diagnostic utility of newer antilatex allergen IgE immunoassays in the present study.

OBJECTIVES: To determine whether an enhanced CAP (ENHCAP) assay or an enzyme-linked immunosorbent assay (ELISA) identifies latex glove symptomatic individuals with antilatex allergen IgE that had not been detected by the CAP assay used in the original study and to determine the specificity of the ENHCAP assay.

METHODS: The ELISA measured IgE antibody to Malaysian nonammoniated natural rubber latex extract (MNA), Hev b1, Hev b5, and Hev b6. Four patient groups were tested: confirmed latex glove allergic, latex glove symptomatic, latex glove sensitized/asymptomatic, and latex glove nonallergic.

RESULTS: The ENHCAP assay and the MNA ELISA were highly concordant with the original CAP assay. In the subgroup with latex glove symptoms that were previously negative by the CAP assay, the ENHCAP assay value was elevated in 7 (11%) of 64 samples, only 3 of which were class 2 or higher. The MNA ELISA result was positive in only 4 (6%) of these 64 samples, and 3 of these were fractionally above the cutoff value for this assay.

CONCLUSIONS: The ENHCAP assay and the MNA ELISA identified a few additional positive individuals in the group that was latex glove symptomatic and originally CAP assay negative. The ENHCAP assay and the MNA ELISA produced only a modest improvement in diagnostic sensitivity over that of the original CAP assay.

Author List

Zeiss CR, Kurup VP, Elms N, Fink JN

MESH terms used to index this publication - Major topics in bold

Antigens, Plant
Health Personnel
Humans
Immunoassay
Immunoglobulin E
Latex
Latex Hypersensitivity
Occupational Diseases
Reproducibility of Results
Sensitivity and Specificity
Veterans