Intramucosal distribution of WNT signaling components in human esophagus. J Clin Gastroenterol 2009 Apr;43(4):327-37
Date
03/25/2009Pubmed ID
19309775Pubmed Central ID
PMC4811354DOI
10.1097/mcg.0b013e31816256ffScopus ID
2-s2.0-65549105061 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
BACKGROUND: The molecular mechanisms governing the biology and pathobiology of esophageal squamous mucosa in health and disease are not completely understood. Earlier genome-wide expression study of normal-looking esophageal squamous mucosa has shown differential expression of the Wingless-type MMTV integration site family (Wnt) modulators Dickkopf (Dkk) homologs among healthy individuals and patients with reflux esophagitis and Barrett metaplasia suggesting that the Wnt pathway may be involved in esophageal mucosal biology.
STUDY: Seven full-thickness human donor esophagi were cryosectioned for immunohistochemical analysis, and lamina propria (LP), basal (BC), intermediate (IC), and superficial (SC) cells were also dissected by laser-capture microdissection for real-time polymerase chain reaction.
RESULTS: Wnt1, 2b, and 3a were expressed primarily in BC, Wnt3, and 5b in LP, and Wnt5a in IC. Frizzled 1, low-density lipoprotein receptor-related protein 6, secreted frizzled-related protein 1, T-cell-specific transcription factor 3, and dishevelled 3 were expressed highest in LP decreasing precipitously medially toward SC. Dkk1 predominantly expressed in SC was more than 100-folds greater than other layers (P<0.001). Dkk4 was expressed primarily in SC but Dkk3 was opposite with greatest expression in LP. Immunohistochemical analysis showed Wnt1 and 3a in BC, Wnt5a in IC and SC, Dkk1 predominantly in SC, Dkk4 in SC and IC, and Dkk3 and SFRP1 in LP and BC
Author List
Ali I, Rafiee P, Zheng Y, Johnson C, Banerjee B, Haasler G, Jacob H, Shaker RAuthors
Banani Banerjee PhD Associate Professor in the Medicine department at Medical College of WisconsinReza Shaker MD Assoc Provost, Sr Assoc Dean, Ctr Dir, Chief, Prof in the Medicine department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Adaptor Proteins, Signal TransducingChemokines
Epithelium
Esophagus
Frizzled Receptors
Gene Expression Profiling
Gene Expression Regulation
Humans
Immunohistochemistry
Intercellular Signaling Peptides and Proteins
Intracellular Signaling Peptides and Proteins
Lasers
Ligands
Membrane Proteins
Microdissection
Mucous Membrane
Polymerase Chain Reaction
Signal Transduction
Transcription Factors
Wnt Proteins
