Developmental regulation of the alpha 1B-adrenoceptor in the sheep kidney. Pediatr Res 1993 Aug;34(2):124-8
Date
08/01/1993Pubmed ID
8233711DOI
10.1203/00006450-199308000-00003Scopus ID
2-s2.0-0027209977 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
The expression of renal alpha 1B-adrenoceptor (alpha 1B-AR) mRNA was studied and contrasted with the expression of renal renin mRNA in fetal and newborn sheep. Fetal sheep between 90 and 91, 116 and 118, and 139 and 141 d gestation (term is 145 d gestation) as well as newborn lambs between 1 and 2 d old and 8 and 10 d old were studied (n = 3 for each age range). The role of the renal nerves in regulating changes in alpha 1B-AR gene expression was also investigated by measuring renal cortical alpha 1B-AR mRNA levels and receptor kd and maximum number of binding sites in 24-h-old lambs that were either denervated (n = 6) or sham-operated (n = 5) 3 d before birth. During development, renal alpha 1B-AR mRNA levels show a marked increase in term fetuses; this increase persists into the first 2 d of life and is distinct from the developmental pattern seen for renal renin mRNA levels. Denervation of term fetuses does not alter the expression of renal alpha 1B-AR mRNA in newborn lambs when compared with sham-operated controls but decreases significantly the expression of the renin gene (p < 0.05). These results suggest that the alpha 1B-AR gene is developmentally regulated in the kidney in a pattern distinct from that seen for renin.(ABSTRACT TRUNCATED AT 250 WORDS)
Author List
Guillery EN, Porter CC, Page WV, Jose PA, Felder R, Robillard JEMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Newborn
Denervation
Female
Fetus
Gene Expression
Kidney
Kidney Cortex
Pregnancy
RNA, Messenger
Receptors, Adrenergic, alpha-1
Renin
Sheep
Sympathetic Nervous System
