CURRICULUM VITAE
Brian C. Smith PhD
Brian C. Smith PhD
Associate Professor
Department of Biochemistry
Associate Director, Program in Chemical Biology
Department of Biochemistry
Associate Director, Program in Chemical Biology
OFFICE ADDRESS: |
Basic Science Building |
8701 Watertown Plank Rd |
Milwaukee, WI 53226 |
EDUCATION: |
09/1999 - 05/2003 B.S., University of Illinois, Urbana-Champaign, IL |
09/2003 - 09/2008 Ph.D., University of Wisconsin, Madison, WI |
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS: |
06/2005 - 08/2005 Internship, Medicinal Chemistry, Genentech, South San Francisco, CA |
09/2008 - 09/2009 Postdoctoral Research Assistant, Biomolecular Chemistry, University of Wisconsin, Madison, WI |
09/2009 - 12/2011 Postdoctoral Research Associate, Chemistry, University of California, Berkeley, CA |
01/2012 - 05/2014 Postdoctoral Research Associate, Chemistry, The Scripps Research Institute, La Jolla, CA |
FACULTY APPOINTMENTS: |
06/01/2014 - Present Assistant Professor, Biochemistry, Medical College of Wisconsin, Milwaukee, WI |
06/01/2016 - Present Adjunct Assistant Professor, Chemistry, Marquette University, Milwaukee, WI |
RESEARCH ADMINISTRATIVE APPOINTMENTS: |
07/2017 - Present Associate Director, Program in Chemical Biology, Medical College of Wisconsin |
AWARDS AND HONORS: |
05/2000 - 05/2003 James Scholar, University of Illinois at Urbana-Champaign |
05/2002 - 08/2002 Snyder Scholar, University of Illinois at Urbana-Champaign |
05/2003 Phi Beta Kappa, University of Illinois at Urbana-Champaign |
05/2003 Bronze Tablet (Top 3% of students in graduating class), University of Illinois at Urbana-Champaign |
08/2003 - 05/2004 Samuel L. McElvain Scholarship, University of Wisconsin-Madison |
12/2018 MCW Outstanding Graduate School Educator Award, MCW Graduate School |
06/2019 MCW Faculty Service Award, MCW Faculty Council |
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES: |
2005 - Present American Chemical Society |
2014 - Present American Heart Association |
2016 - Present American Society for Biochemistry and Molecular Biology |
2016 - Present Society for Redox Biology and Medicine |
2017 - Present American Diabetes Association |
2018 - Present American Physiological Society |
EDITORSHIPS/EDITORIAL BOARDS/JOURNAL REVIEWS: |
Editorial Board |
2014 - Present American Journal of Physiology - Regulatory, Integrative and Comparative Physiology |
Journal Review |
2014 - Present Biochemistry |
2014 - Present Journal of Biological Chemistry |
2014 - Present ChemMedChem |
2015 - Present Nitric Oxide |
2015 - Present Nature Communications |
2015 - Present Journal of the American Chemical Society |
2015 - Present Nature Chemical Biology |
2017 - Present ACS Chemical Biology |
2017 - Present Biochimie |
2017 - Present Journal of Inorganic Biochemistry |
2018 - Present Frontiers in Bioscience |
2018 - Present Cell Chemical Biology |
2018 - Present Proteins |
2018 - Present Oncotarget |
2019 - Present Journal of Natural Products |
LOCAL/REGIONAL APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
2005 - 2008 Graduate student member, Biotechnology Center Peptide Synthesis Steering Committee, University of Wisconsin-Madison |
RESEARCH GRANTS/AWARDS/CONTRACTS/PROJECTS: | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
INVITED LECTURES/WORKSHOPS/PRESENTATIONS: |
International |
Brian C. Smith, Michael D. Olp, Daniel J. Sprague, Discovering and Exploiting Selectivity in BET Tandem Bromodomain Recognition of Epigenetic Lysine Acylation, American Society for Biochemistry and Molecular Biology, San Diego, CA, 04/21/2018 - 04/25/2018 |
Brian C. Smith, Michael D. Olp, Daniel J. Sprague, Stefan G. Kathman, Ziyang Xu, Alexander V. Statsyuk, Vaughn Jackson, Discovering and Exploiting Selectivity in BET Tandem Bromodomain Recognition of Epigenetic Lysine Acylation, Reversible Protein Acetylation in Health and Disease, Lisbon, Portugal, 08/04/2019 - 08/09/2019 |
National |
Brian C. Smith and John M. Denu, Acetyl-lysine analogs as mechanistic probes of protein deacetylases, ASBMB Summer Research Conference on Histone Deacetylases, Snowmass Village, CO, 06/02/2007 - 06/07/2007 |
Brian C. Smith, Control of protein function through post-translational modification: sirtuin deacetylases and cysteine S-nitrosation, Purdue University, 12/11/2012 |
Brian C. Smith, Erasing and Reading Epigenetic Lysine Acylation with Sirtuins and Bromodomains, Department of Chemistry seminar series, Wake Forest University, 03/01/2017 |
Brian C. Smith, Erasing and Reading Epigenetic Lysine Acylation with Sirtuins and Bromodomains, Department of Chemistry seminar series, University of Kansas, 03/22/2019 |
Regional |
Brian C. Smith, Melody G. Campbell, Eric S. Underbakke, Daniel W. Kulp, William R. Schief, Clinton S. Potter, Bridget Carragher, Michael A. Marletta, Nitric Oxide Synthase Molecular Architecture and Domain Interfaces Regulate Electron Transfer and Calmodulin Activation, The 34th Midwest Enzyme Chemistry Conference, Northwestern University, 09/27/2014 |
Brian C. Smith, Reading and Erasing Epigenetic Lysine Acylation with Bromodomains and Sirtuins, Pharmacy school seminar series, Concordia University, 12/02/2015 |
Brian C. Smith, Erasing and Reading Epigenetic Lysine Acylation with Sirtuins and Bromodomains, Department of Chemistry seminar series, Marquette University, 09/02/2016 |
Brian C. Smith, Erasing and Reading Epigenetic Lysine Acylation with Sirtuins and Bromodomains, Department of Biology seminar series, Hope College, 09/16/2016 |
Brian C. Smith, Erasing and Reading Epigenetic Lysine Acylation with Sirtuins and Bromodomains, Department of Chemistry & Biochemistry seminar series, University of Wisconsin-La Crosse, 04/14/2017 |
Brian C. Smith, Erasing and Reading Epigenetic Lysine Acylation with Sirtuins and Bromodomains, Department of Chemistry seminar series, University of Wisconsin-Milwaukee, 05/05/2017 |
Sarah L. Wynia-Smith, Brian C. Smith, Science Building Dedication, Northwestern College, 09/28/2018 |
Local |
Brian C. Smith, Biological Mechanisms of Sirtuin Deacetylases and Nitric Oxide Synthases, Department of Biochemistry seminar series, Medical College of Wisconsin, 10/16/2013 |
Brian C. Smith, Sarah L. Wynia-Smith, Yani Zhou, Charles Knutson, Shalise Couvertier, Nathaniel Fernhoff, John Wishnok, Steven Tannenbaum, Eranthie Weerapana, and Michael A. Marletta, Nitric Oxide Signaling through S-Nitrosoglutathione Transnitrosation, Cardiovascular Redox Signaling Symposium, Medical College of Wisconsin, 11/14/2014 |
Brian C. Smith, Direct and Epigenetic Control of Metabolism through Protein S-nitrosation, Redox Biology Work in Progress, Medical College of Wisconsin, 06/04/2015 |
Brian C. Smith, Erasing Epigenetic Lysine Acylation with Bromodomains and Sirtuins & Mechanisms and Pathogenesis of Gasotransmittors, Department of Biochemistry seminar series, Medical College of Wisconsin, 11/11/2015 |
Brian C. Smith, Selective inhibitors and binding targets of the BRD4 bromodomains, Cancer Center symposium, Medical College of Wisconsin, 12/02/2015 |
Brian C. Smith, Discovering and Engineering Selectivity among BET Bromodomains to Treat Cancer, Cancer Cell Biology seminar series, Medical College of Wisconsin, 05/19/2016 |
Brian C. Smith, Erasing and Reading Epigenetic Lysine Acylation with Sirtuins and Bromodomains, Department of Biophysics seminar series, Medical College of Wisconsin, 04/27/2017 |
Brian C. Smith, Michael D. Olp, Integration of Chemical Biology and Mass Spectrometry to Study Post-translational Modification by Cysteine S-nitrosation and Lysine Acylation, Mass Spectrometry Discussion Group, Medical College of Wisconsin, 09/07/2017 |
Brian C. Smith, Software tools for team collaboration, communication, and organization, AHW Learning Community, Medical College of Wisconsin, 01/30/2018 |
Brian C. Smith, Reading and Erasing Epigenetic Lysine Acylation with Bromodomains and Sirtuins, Department of Physiology seminar series, Medical College of Wisconsin, 03/14/2018 |
Brian C. Smith, Brian F. Volkman, Structure-Based Drug Discovery, Focus on Technology seminar series, Blood Research Institute, 07/10/2018 |
Brian C. Smith, Chemical Biology of Sirtuins and Bromodomains, MCW Cardiovascular Center – ATVB Signature Program, Medical College of Wisconsin, 07/31/2018 |
Brian F. Volkman, Brian C. Smith, Structure-based drug discovery at MCW, MCW Cancer Center - Discovery & Developmental Therapeutics Program Seminar, Medical College of Wisconsin, 11/30/2018 |
Brian C. Smith, Discovering and Exploiting Selectively in BET Bromodomains in the Context of Type I Diabetes, Department of Obstetrics and Gynecology seminar series, Medical College of Wisconsin, 04/17/2019 |
Brian C. Smith, Regulation of Nuclear Sirtuin Histone Deacylase Activity by Cellular Oxidants, Cardiovascular Center – Redox Biology affinity group, Medical College of Wisconsin, 04/18/2019 |
PEER REVIEWED WORKSHOPS/PRESENTATIONS: |
National |
Mary K. Smalley, Brian C. Smith, Scott K. Silverman, Pyrene fluorescence as a general RNA folding probe, Ninth Annual Meeting of the RNA Society, Madison, WI, 06/01/2004 - 06/06/2004 |
Brian C. Smith and John M. Denu, Chemical mechanism of Sir2 NAD-dependent protein deacetylases, 231st ACS National Meeting, Atlanta, GA, 03/26/2006 - 03/30/2006 |
Brian C. Smith and Michael A. Marletta, Transnitrosation signaling pathways initiated by S-nitrosation of the nitric oxide synthase Zn2+-tetrathiolate, 243rd ACS National Meeting & Exposition, San Diego, CA, 03/25/2012 - 03/29/2012 |
Brian C. Smith and Michael A. Marletta, Mechanisms of electron transfer and auto-S-nitrosation in nitric oxide synthases, Enzymes, Coenzymes, & Metabolic Pathways Gordon Research Conference, Waterville Valley Resort, NH, 07/15/2012 - 07/20/2012 |
COMMITTEE SERVICE: |
Medical College of Wisconsin |
09/2014 - 05/2015 Faculty member, Redox Biology Program Faculty Search Committee, Redox Biology Program, Medical College of Wisconsin |
10/2014 - Present Faculty member, Redox Biology Program Symposium Planning Committee, Redox Biology Program, Medical College of Wisconsin |
10/2014 - Present Faculty member, Shared Biophysical Research Instrumentation Committee, Department of Biochemistry, Medical College of Wisconsin |
05/2016 - Present Alternate for Dr. Volkman, Research Computing Advisory Committee, Medical College of Wisconsin |
11/2016 - 01/2017 Faculty member, Faculty Information Technology Research Subcommittee, Medical College of Wisconsin |
11/2016 - 06/2018 Department of Biochemistry representative, Committee on Curriculum Development, Interdisciplinary Doctoral Program in Biomedical Sciences, Medical College of Wisconsin |
05/2017 - 02/2019 Vice Chair, Program in Chemical Biology Faculty Search Committee, Department of Biochemistry, Medical College of Wisconsin |
07/2017 - 06/2020 Vice Chair, Information Technology Committee, Medical College of Wisconsin |
09/2017 - Present Organizer, Redox Signaling Journal Club, Departments of Biochemistry & Biophysics, Medical College of Wisconsin |
12/2018 - Present Faculty member, Biochemistry Graduate Student Committee, Department of Biochemistry, Medical College of Wisconsin |
12/2018 - Present Department of Biochemistry representative, Recruitment Committee, Interdisciplinary Doctoral Program in Biomedical Sciences, Medical College of Wisconsin |
12/2018 - 05/2019 Department of Biochemistry representative, Boot Camp Work Group, Interdisciplinary Doctoral Program in Biomedical Sciences, Medical College of Wisconsin |
05/2019 - Present Faculty member, Orchestra Committee, Medical College of Wisconsin |
08/2019 - Present Faculty member, DSHREP Steering Committee, Office of Diversity and Inclusion, Office of Academic Affairs, Medical College of Wisconsin |
09/2019 - Present Faculty member, Ambassador Program Work Group, Graduate School, Medical College of Wisconsin |
09/2019 - Present Faculty member, Research Strategic Plan Steering Committee – Data Science Workgroup, Research Council, Medical College of Wisconsin |
MCW TEACHING ACTIVITIES: |
Medical Student Education |
2015 - Present Molecules to Cells - Case-based discussions (three 2 hour sessions) |
2017 - Present Molecules to Cells - Enzyme Kinetics and Catalysis lectures (three 1 hour lectures) |
Graduate Student Education |
2015 - 2018 Biochemistry 02222 - Advanced Protein Chemistry (three 90 minute lectures) |
2017 Pharmacology and Toxicology 07237A - Modern Drug Discovery and Development (one 3 hour lecture) |
2018 - 2019 Biochemistry 02276A - Special Topics in Biochemistry: Mass Spectrometry in Biomedical Research (one 90 minute lecture) |
2019 - Present Techniques in Molecular & Cellular Biology (co-Course director) |
2019 - Present Professional Development I (two 1 hour lectures) |
2019 - Present Foundations in Biomedical Sciences II (one 1 hour lecture) |
2019 - Present Biochemistry 02301-0 - Seminar (Course director) |
2019 - Present Foundations in Biomedical Sciences I (one 2 hour lecture; three 1 hour lectures) |
MCW STUDENTS, FACULTY, RESIDENTS AND CLINICAL/RESEARCH FELLOWS MENTORED: |
Medical Students |
09/2015 - 06/2019 Tom Hirsch, Pathways advisor, Medical College of Wisconsin, 2016 Medical Student Research Day Poster Winner
Currently a Surgery Resident, Beth Israel Hospital, Boston, MA |
Graduate Students |
PhD Students Advised |
07/2014 - 06/2019 Michael Olp, Regulation and Functional Significance of Chromatin Binding by BET Tandem Bromodomains, Medical College of Wisconsin, MCW 26th annual graduate student poster session award winner, 10/6/2016
MCW Mass Spectrometry Symposium poster session award winner, 6/2/2017 |
01/2015 - 03/2015 Anthony Brandt, Rotation Student, Medical College of Wisconsin, Currently a graduate student in the laboratory of Carol Williams |
03/2015 - Present Kelsey Kalous, Medical College of Wisconsin, NIDDK F31 fellowship award (Impact score 12)
AHA Pre-doctoral fellowship (declined)
Young Investigator Award, Redox Biology Symposium, MCW, 4/13/2018
Young Investigator Award, 14th Annual Chicago Diabetes Day, 5/4/2019 |
08/2015 - 10/2015 Tanner Lundquist, Rotation Student, Medical College of Wisconsin, Currently a chemist at Land O' Lakes, Minneapolis, MN |
05/2016 - Present Christopher Goetz, Medical College of Wisconsin |
05/2019 - Present Alyssa Fernandez, Medical College of Wisconsin |
08/2019 - 09/2019 Demetra Korkos, Rotation Student, Medical College of Wisconsin |
09/2019 - 10/2019 Laura Danner, Rotation Student, Medical College of Wisconsin |
09/2019 - 10/2019 Raymundo Nunez, Rotation Student, Medical College of Wisconsin |
PhD Committees |
2015 - 02/19/2019 Maxx Tessmer, Biophysical and Computational Characterization of the ExoU Activation Mechanism, Medical College of Wisconsin, Currently at Postdoctoral Research Scientist at University of Washington |
2015 - 03/27/2018 Sarah Riutta, Structure-function studies of the human chemokine CCL20 and investigation of its viability as a therapeutic target, Medical College of Wisconsin, Currently a Senior Research Associate at Advocate Aurora Health, Milwaukee, WI |
2015 - 06/13/2019 John Egner, Discovery of a novel conformation of human Fis1 important for ligand binding and activity, Medical College of Wisconsin |
2015 - 12/13/2018 Stephanie Santarriaga, Medical College of Wisconsin, Currently a research fellow at Massachusetts General Hospital |
2015 - 04/15/2019 Andrew Kleist, Signal Discrimination and Signal Tranduction at Chemokine G Protein-Coupled Receptors, Medical College of Wisconsin, Currently an M3 medical student at MCW |
09/2017 - Present Chay Teng (Zoe) Yeo, Medical College of Wisconsin |
2018 - Present Acacia Dishman, Medical College of Wisconsin |
2019 - Present Joshua Stafford, Medical College of Wisconsin |
2019 - Present Kevin Jennings, Medical College of Wisconsin |
2019 - Present Sam DeCero, Medical College of Wisconsin |
Postdoctoral Researchers |
10/2016 - Present Daniel Sprague, Medical College of Wisconsin |
07/2018 - 08/2019 Moua Yang, Medical College of Wisconsin, Funded by the Cardiovascular Center T32 postdoctoral training program, “Training in Signature Transdisciplinary Cardiovascular Sciences” |
07/2019 - Present Joshua Nord, Medical College of Wisconsin |
09/2019 - Present Shifali Shishodia, Medical College of Wisconsin |
09/2019 - Present Rachel Jones Lipinski, Medical College of Wisconsin |
Faculty |
09/2019 - Present Emma Morrison, Member of mentoring committee, Medical College of Wisconsin |
EXTRAMURAL STUDENTS, FACULTY, RESIDENTS, AND CLINICAL/RESEARCH FELLOWS MENTORED: |
High School Students |
01/2019 - 08/2019 Isabel Szablewski, Advisor, Brookfield East High School, Currently an undergraduate science major at Carroll University |
Undergraduate Students |
02/2010 - 05/2011 Andrew Birnberg, University of California-Berkeley, Currently a Ph.D. level Bioinformatics Engineer at Genentech |
10/2015 - 08/2018 Brad Julga, PURE, SPUR, MSSRP advisor, Carroll University, Medical College of Wisconsin, Currently a medical student at MCW |
01/2016 - 04/2016 Vincent Wartenweiler, PURE advisor, Carroll University, Currently a pharmacy student at UW-Madison |
06/04/2018 - Present Guadalupe Valdivia Esparza, DSHREP/SURE advisor, Marquette University |
06/04/2018 - 08/03/2018 Nnenna Nwaelugo, SPUR advisor, Northwestern College, Currently a medical student at MCW |
Mark Devries, University of Wisconsin-Madison |
Graduate Students |
09/2010 - 02/2011 Peter 't Hart, Rotation Student, University of California-Berkeley |
11/2010 - 02/2011 Peyton Shieh, Rotation Student, University of California-Berkeley |
02/2011 - 05/2011 Minxi Rao, Rotation Student, University of California-Berkeley |
Jorg Schemies, University of Wisconsin-Madison |
Kunil Raval, Rotation Student, University of Wisconsin-Madison |
Ye Jin Eun, Rotation Student, University of Wisconsin-Madison |
MS Students Advised |
06/2016 - 09/2016 Nicole Walls, DSHREP advisor, Southern Illinois University at Carbondale, Currently a medical student at Loyola University |
06/2017 - 08/2017 Karael Campbell, DSHREP advisor, Southern Illinois University at Carbondale, Currently a medical student at Heritage College of Osteopathic Medicine - Ohio University |
Postdoctoral Researchers |
01/2018 - 07/2018 Zuzana Gulasova, Visiting postdoctoral scholar, Pavol Jozef Šafárik University |
COMMUNITY SERVICE ACTIVITIES: |
10/2017 - Present Leader of K5 to 2nd grade Destination Imagination teams, Tonawanda Elementary School |
03/08/2018 Science Fair Judge, Tonawanda Elementary School |
03/07/2019 Science Fair Judge, Tonawanda Elementary School |
BIBLIOGRAPHY |
Refereed Journal Publications/Original Papers |
1. Schmidt MT, Smith BC, Jackson MD, Denu JM. Coenzyme specificity of Sir2 protein deacetylases: implications for physiological regulation. J Biol Chem. 2004 Sep 17;279(38):40122-9. |
2. Borra MT, Smith BC, Denu JM. Mechanism of human SIRT1 activation by resveratrol. J Biol Chem. 2005 Apr 29;280(17):17187-95. |
3. Smith BC, Denu JM. Sir2 protein deacetylases: evidence for chemical intermediates and functions of a conserved histidine. Biochemistry. 2006 Jan 10;45(1):272-82. PMCID: PMC2519119 |
4. Smith BC, Denu JM. Sir2 deacetylases exhibit nucleophilic participation of acetyl-lysine in NAD+ cleavage. J Am Chem Soc. 2007 May 09;129(18):5802-3. PMCID: PMC2568996 |
5. Smith BC, Denu JM. Acetyl-lysine analog peptides as mechanistic probes of protein deacetylases. J Biol Chem. 2007 Dec 21;282(51):37256-65. |
6. Smith BC, Denu JM. Mechanism-based inhibition of Sir2 deacetylases by thioacetyl-lysine peptide. Biochemistry. 2007 Dec 18;46(50):14478-86. |
7. Smith BC, Hallows WC, Denu JM. A continuous microplate assay for sirtuins and nicotinamide-producing enzymes. Anal Biochem. 2009 Nov 01;394(1):101-9. PMCID: PMC2752052 |
8. Smith BC, Settles B, Hallows WC, Craven MW, Denu JM. SIRT3 substrate specificity determined by peptide arrays and machine learning. ACS Chem Biol. 2011 Feb 18;6(2):146-57. PMCID: PMC3042044 |
9. Hallows WC, Yu W, Smith BC, Devries MK, Ellinger JJ, Someya S, Shortreed MR, Prolla T, Markley JL, Smith LM, Zhao S, Guan KL, Denu JM. Sirt3 promotes the urea cycle and fatty acid oxidation during dietary restriction. Mol Cell. 2011 Jan 21;41(2):139-49. PMCID: PMC3101115 |
10. Kasamatsu A, Nakao M, Smith BC, Comstock LR, Ono T, Kato J, Denu JM, Moss J. Hydrolysis of O-acetyl-ADP-ribose isomers by ADP-ribosylhydrolase 3. J Biol Chem. 2011 Jun 17;286(24):21110-7. PMCID: PMC3122172 |
11. Chen Y, Yang M, Huang W, Chen W, Zhao Y, Schulte ML, Volberding P, Gerbec Z, Zimmermann MT, Zeighami A, Demos W, Zhang J, Knaack DA, Smith BC, Cui W, Malarkannan S, Sodhi K, Shapiro JI, Xie Z, Sahoo D, Silverstein RL. Mitochondrial Metabolic Reprogramming by CD36 Signaling Drives Macrophage Inflammatory Responses. Circ Res. 2019 Dec 06;125(12):1087-1102. PMCID: PMC6921463 |
12. Smith BC, Anderson MA, Hoadley KA, Keck JL, Cleland WW, Denu JM. Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae. Biochemistry. 2012 Jan 10;51(1):243-56. PMCID: PMC3257521 |
13. Smith BC, Fernhoff NB, Marletta MA. Mechanism and kinetics of inducible nitric oxide synthase auto-S-nitrosation and inactivation. Biochemistry. 2012 Feb 07;51(5):1028-40. PMCID: PMC3277664 |
14. Smith BC, Underbakke ES, Kulp DW, Schief WR, Marletta MA. Nitric oxide synthase domain interfaces regulate electron transfer and calmodulin activation. Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):E3577-86. PMCID: PMC3780838 |
15. Campbell MG, Smith BC, Potter CS, Carragher B, Marletta MA. Molecular architecture of mammalian nitric oxide synthases. Proc Natl Acad Sci U S A. 2014 Sep 02;111(35):E3614-23. PMCID: PMC4156747 |
16. Shteyer E, Edvardson S, Wynia-Smith SL, Pierri CL, Zangen T, Hashavya S, Begin M, Yaacov B, Cinamon Y, Koplewitz BZ, Vromen A, Elpeleg O, Smith BC. Truncating mutation in the nitric oxide synthase 1 gene is associated with infantile achalasia. Gastroenterology. 2015 Mar;148(3):533-536.e4. |
17. Rao M, Smith BC, Marletta MA. Nitric Oxide Mediates Biofilm Formation and Symbiosis in Silicibacter sp. Strain TrichCH4B. mBio. 2015 May 05;6(3):e00206-15. PMCID: PMC4436077 |
18. Kalous KS, Wynia-Smith SL, Olp MD, Smith BC. Mechanism of Sirt1 NAD+-dependent Protein Deacetylase Inhibition by Cysteine S-Nitrosation. J Biol Chem. 2016 Dec 02;291(49):25398-25410. PMCID: PMC5207242 |
19. Zhou Y, Wynia-Smith SL, Couvertier SM, Kalous KS, Marletta MA, Smith BC, Weerapana E. Chemoproteomic Strategy to Quantitatively Monitor Transnitrosation Uncovers Functionally Relevant S-Nitrosation Sites on Cathepsin D and HADH2. Cell Chem Biol. 2016 Jun 23;23(6):727-37. PMCID: PMC4920707 |
20. Olp MD, Zhu N, Smith BC. Metabolically Derived Lysine Acylations and Neighboring Modifications Tune the Binding of the BET Bromodomains to Histone H4. Biochemistry. 2017 Oct 17;56(41):5485-5495. PMCID: PMC5970795 |
21. Bruegger JJ, Smith BC, Wynia-Smith SL, Marletta MA. Comparative and integrative metabolomics reveal that <i>S</i>-nitrosation inhibits physiologically relevant metabolic enzymes. J Biol Chem. 2018 Apr 27;293(17):6282-6296. PMCID: PMC5925818 |
22. Egner JM, Jensen DR, Olp MD, Kennedy NW, Volkman BF, Peterson FC, Smith BC, Hill RB. Development and Validation of 2D Difference Intensity Analysis for Chemical Library Screening by Protein-Detected NMR Spectroscopy. Chembiochem. 2018 Mar 02;19(5):448-458. PMCID: PMC5844354 |
23. Hanson QM, Carley JR, Gilbreath TJ, Smith BC, Underbakke ES. Calmodulin-induced Conformational Control and Allostery Underlying Neuronal Nitric Oxide Synthase Activation. J Mol Biol. 2018 Mar 30;430(7):935-947. |
24. Fahey JM, Stancill JS, Smith BC, Girotti AW. Nitric oxide antagonism to glioblastoma photodynamic therapy and mitigation thereof by BET bromodomain inhibitor JQ1. J Biol Chem. 2018 Apr 06;293(14):5345-5359. PMCID: PMC5892570 |
Books, Chapters, and Reviews |
1. Grubisha O, Smith BC, Denu JM. Small molecule regulation of Sir2 protein deacetylases. FEBS J. 2005 Sep;272(18):4607-16. |
2. Smith BC, Denu JM. Sirtuins caught in the act. Structure. 2006 Aug;14(8):1207-8. |
3. Garske AL, Smith BC, Denu JM. Linking SIRT2 to Parkinson's disease. ACS Chem Biol. 2007 Aug 17;2(8):529-32. |
4. Smith BC, Hallows WC, Denu JM. Mechanisms and molecular probes of sirtuins. Chem Biol. 2008 Oct 20;15(10):1002-13. PMCID: PMC2626554 |
5. Smith BC, Denu JM. Chemical mechanisms of histone lysine and arginine modifications. Biochim Biophys Acta. 2009 Jan;1789(1):45-57. PMCID: PMC2642981 |
6. Hallows WC, Smith BC, Lee S, Denu JM. Ure(k)a! Sirtuins Regulate Mitochondria. Cell. 2009 May 01;137(3):404-6. PMCID: PMC3310393 |
7. Smith BC, Marletta MA. Mechanisms of S-nitrosothiol formation and selectivity in nitric oxide signaling. Curr Opin Chem Biol. 2012 Dec;16(5-6):498-506. PMCID: PMC3518608 |
8. Surmeli NB, Underbakke ES, Smith BC, Wynia-Smith SL, Marletta MA. “Nitric oxide signaling”. Comprehensive Inorganic Chemistry II. 2013, Vol. 3, 241-262. |
9. Wynia-Smith SL, Smith BC. Nitrosothiol formation and S-nitrosation signaling through nitric oxide synthases. Nitric Oxide. 2017 Feb 28;63:52-60. |
10. McCusker P, Mian MY, Li G, Olp MD, Tiruveedhula VVNPB, Rashid F, Golani LK, Verma RS, Smith BC, Cook JM, Chan JD. Non-sedating benzodiazepines cause paralysis and tissue damage in the parasitic blood fluke Schistosoma mansoni. PLoS Negl Trop Dis. 2019 Nov;13(11):e0007826. PMCID: PMC6881066 |
11. Olp MD, Sprague DJ, Goetz CJ, Kathman SG, Wynia-Smith SL, Shishodia S, Summers SB, Xu Z, Statsyuk AV, Smith BC. Covalent-Fragment Screening of BRD4 Identifies a Ligandable Site Orthogonal to the Acetyl-Lysine Binding Sites. ACS Chem Biol. 2020 Apr 17;15(4):1036-1049. PMCID: PMC7271629 |
12. Kalous KS, Wynia-Smith SL, Summers SB, Smith BC. Human sirtuins are differentially sensitive to inhibition by nitrosating agents and other cysteine oxidants. J Biol Chem. 2020 Jun 19;295(25):8524-8536. PMCID: PMC7307208 |
13. Kapoor A, Ghosh AK, Forman M, Hu X, Ye W, Southall N, Marugan J, Keyes RF, Smith BC, Meyers DJ, Ferrer M, Arav-Boger R. Validation and Characterization of Five Distinct Novel Inhibitors of Human Cytomegalovirus. J Med Chem. 2020 Apr 23;63(8):3896-3907. PMCID: PMC7386824 |
14. Olp MD, Kalous KS, Smith BC. ICEKAT: an interactive online tool for calculating initial rates from continuous enzyme kinetic traces. BMC Bioinformatics. 2020 May 14;21(1):186. PMCID: PMC7222511 |
15. Yang M, Li W, Harberg C, Chen W, Yue H, Ferreira RB, Wynia-Smith SL, Carroll KS, Zielonka J, Flaumenhaft R, Silverstein RL, Smith BC. Cysteine sulfenylation by CD36 signaling promotes arterial thrombosis in dyslipidemia. Blood Adv. 2020 Sep 22;4(18):4494-4507. PMCID: PMC7509873 |
16. Goetz CJ, Sprague DJ, Smith BC. Development of activity-based probes for the protein deacylase Sirt1. Bioorg Chem. 2020 Nov;104:104232. PMCID: PMC7686039 |
17. Chi YI, Stodola TJ, De Assuncao TM, Leverence EN, Tripathi S, Dsouza NR, Mathison AJ, Basel DG, Volkman BF, Smith BC, Lomberk G, Zimmermann MT, Urrutia R. Molecular mechanics and dynamic simulations of well-known Kabuki syndrome-associated KDM6A variants reveal putative mechanisms of dysfunction. Orphanet J Rare Dis. 2021 Feb 05;16(1):66. PMCID: PMC7866879 |
18. Jones Lipinski RA, Thillier Y, Morisseau C, Sebastiano CS Jr, Smith BC, Hall CD, Katritzky AR. Molecular docking-guided synthesis of NSAID-glucosamine bioconjugates and their evaluation as COX-1/COX-2 inhibitors with potentially reduced gastric toxicity. Chem Biol Drug Des. 2021 Jul;98(1):102-113. PMCID: PMC8281356 |
19. Chi YI, Stodola TJ, De Assuncao TM, Leverence EN, Tripathi S, Dsouza NR, Mathison AJ, Basel DG, Volkman BF, Smith BC, Lomberk G, Zimmermann MT, Urrutia R. Correction to: Molecular mechanics and dynamic simulations of well-known Kabuki syndrome-associated KDM6A variants reveal putative mechanisms of dysfunction. Orphanet J Rare Dis. 2021 Jun 01;16(1):247. PMCID: PMC8170813 |
20. Stodola TJ, Chi YI, De Assuncao TM, Leverence EN, Tripathi S, Dsouza NR, Mathison AJ, Volkman BF, Smith BC, Lomberk G, Zimmermann MT, Urrutia R. Computational modeling reveals key molecular properties and dynamic behavior of disruptor of telomeric silencing 1-like (DOT1L) and partnering complexes involved in leukemogenesis. Proteins. 2022 Jan;90(1):282-298. PMCID: PMC8671179 |
21. Sprague DJ, Getschman AE, Fenske TG, Volkman BF, Smith BC. Trisubstituted 1,3,5-Triazines: The First Ligands of the sY12-Binding Pocket on Chemokine CXCL12. ACS Med Chem Lett. 2021 Nov 11;12(11):1773-1782. PMCID: PMC8592115 |
22. Kanack AJ, Olp MD, Newsom OJ, Scaglione JB, Gooden DM, McMahon K, Smith BC, Scaglione KM. Chemical Regulation of the Protein Quality Control E3 Ubiquitin Ligase C-Terminus of Hsc70 Interacting Protein (CHIP). Chembiochem. 2022 Mar 18;23(6):e202100633. PMCID: PMC9016715 |
23. Kalous KS, Wynia-Smith SL, Smith BC. Sirtuin Oxidative Post-translational Modifications. Front Physiol. 2021;12:763417. PMCID: PMC8652059 |
24. Jones Lipinski RA, Stancill JS, Wynia-Smith SL, Sprague DJ, Nunez R, Bird A, Corbett JA, Smith BC. Zinc-Chelating BET Bromodomain Inhibitors Selectively Accumulate and Affect Gene Expression in Pancreatic β-Cells. FASEB J. 2022 May;36 Suppl 1. |
25. Fisher CL, Wan TC, Fallot LB, Keyes RF, Suresh RR, Rothwell AC, Gao ZG, McCorvy JD, Smith BC, Jacobson KA, Auchampach JA. Characterization of Novel A<sub>3</sub> Adenosine Receptor Allosteric Modulators. FASEB J. 2022 May;36 Suppl 1. |
26. Johnston N, Samuels TL, Goetz CJ, Arnold LA, Smith BC, Seabloom D, Wuertz B, Ondrey F, Wiedmann TS, Vuksanovic N, Silvaggi NR, MacKinnon AC, Miller J, Bock J, Blumin JH. Oral and Inhaled Fosamprenavir Reverses Pepsin-Induced Damage in a Laryngopharyngeal Reflux Mouse Model. Laryngoscope. 2023 Jan;133 Suppl 1(Suppl 1):S1-S11. PMCID: PMC9732152 |
27. Chi YI, Stodola TJ, De Assuncao TM, Leverence EN, Smith BC, Volkman BF, Mathison AJ, Lomberk G, Zimmermann MT, Urrutia R. Structural bioinformatics enhances the interpretation of somatic mutations in KDM6A found in human cancers. Comput Struct Biotechnol J. 2022;20:2200-2211. PMCID: PMC9111933 |
28. Nord JA, Wynia-Smith SL, Gehant AL, Jones Lipinski RA, Naatz A, Rioja I, Prinjha RK, Corbett JA, Smith BC. <i>N-</i>terminal BET bromodomain inhibitors disrupt a BRD4-p65 interaction and reduce inducible nitric oxide synthase transcription in pancreatic β-cells. Front Endocrinol (Lausanne). 2022;13:923925. PMCID: PMC9513428 |
29. Fisher CL, Fallot LB, Wan TC, Keyes RF, Suresh RR, Rothwell AC, Gao ZG, McCorvy JD, Smith BC, Jacobson KA, Auchampach JA. Characterization of Dual-Acting A<sub>3</sub> Adenosine Receptor Positive Allosteric Modulators That Preferentially Enhance Adenosine-Induced Gα<sub>i3</sub> and Gα<sub>oA</sub> Isoprotein Activation. ACS Pharmacol Transl Sci. 2022 Aug 12;5(8):625-641. PMCID: PMC9380209 |
30. Shishodia S, Nuñez R, Strohmier BP, Bursch KL, Goetz CJ, Olp MD, Jensen DR, Fenske TG, Ordonez-Rubiano SC, Blau ME, Roach MK, Peterson FC, Volkman BF, Dykhuizen EC, Smith BC. Selective and Cell-Active PBRM1 Bromodomain Inhibitors Discovered through NMR Fragment Screening. J Med Chem. 2022 Oct 27;65(20):13714-13735. PMCID: PMC9630929 |
31. Wang X, Wan TC, Kulik KR, Lauth A, Smith BC, Lough JW, Auchampach JA. Pharmacological inhibition of the acetyltransferase Tip60 mitigates myocardial infarction injury. Dis Model Mech. 2023 May 01;16(5). PMCID: PMC9672930 |
32. AbuEid M, Keyes RF, McAllister D, Peterson F, Kadamberi IP, Sprague DJ, Chaluvally-Raghavan P, Smith BC, Dwinell MB. Fluorinated triphenylphosphonium analogs improve cell selectivity and <i>in vivo</i> detection of mito-metformin. iScience. 2022 Dec 22;25(12):105670. PMCID: PMC9768319 |
33. Yang M, Chiu J, Scartelli C, Ponzar N, Patel S, Patel A, Ferreira RB, Keyes RF, Carroll KS, Pozzi N, Hogg PJ, Smith BC, Flaumenhaft R. Sulfenylation links oxidative stress to protein disulfide isomerase oxidase activity and thrombus formation. J Thromb Haemost. 2023 Aug;21(8):2137-2150. PMCID: PMC10657653 |
34. Ghosh AK, Su YP, Forman M, Keyes RF, Smith BC, Hu X, Ferrer M, Arav-Boger R. Harnessing the Noncanonical Keap1-Nrf2 Pathway for Human Cytomegalovirus Control. J Virol. 2023 Apr 27;97(4):e0016023. PMCID: PMC10134830 |
35. Zhou AL, Jensen DR, Peterson FC, Thomas MA, Schlimgen RR, Dwinell MB, Smith BC, Volkman BF. Fragment-based drug discovery of small molecule ligands for the human chemokine CCL28. SLAS Discov. 2023 Jun;28(4):163-169. PMCID: PMC10339762 |
36. Yang M, Smith BC. Cysteine and methionine oxidation in thrombotic disorders. Curr Opin Chem Biol. 2023 Oct;76:102350. PMCID: PMC10527720 |
37. Ratnasinghe BD, Haque N, Wagenknecht JB, Jensen DR, Esparza GV, Leverence EN, De Assuncao TM, Mathison AJ, Lomberk G, Smith BC, Volkman BF, Urrutia R, Zimmermann MT. Beyond Structural Bioinformatics for Genomics with Dynamics Characterization of an Expanded KRAS Mutational Landscape. bioRxiv. 2023 Apr 28. PMCID: PMC10189839 |
38. Chi YI, Jorge SD, Jensen DR, Smith BC, Volkman BF, Mathison AJ, Lomberk G, Zimmermann MT, Urrutia R. A Multi-Layered Computational Structural Genomics Approach Enhances Domain-Specific Interpretation of Kleefstra Syndrome Variants in EHMT1. bioRxiv. 2023 Sep 07. PMCID: PMC10541560 |
39. Keyes RF, McAllister D, Dwinell MB, Smith BC. Synthesis of fluorinated triphenylphosphonium analogs that improve cancer cell selectivity and in vivo detection. STAR Protoc. 2023 Sep 15;4(3):102437. PMCID: PMC10424135 |
40. Ordonez-Rubiano SC, Maschinot CA, Wang S, Sood S, Baracaldo-Lancheros LF, Strohmier BP, McQuade AJ, Smith BC, Dykhuizen EC. Rational Design and Development of Selective BRD7 Bromodomain Inhibitors and Their Activity in Prostate Cancer. J Med Chem. 2023 Aug 24;66(16):11250-11270. PMCID: PMC10641717 |
41. Jorge SD, Chi YI, Mazaba JL, Haque N, Wagenknecht J, Smith BC, Volkman BF, Mathison AJ, Lomberk G, Zimmermann MT, Urrutia R. Deep computational phenotyping of genomic variants impacting the SET domain of KMT2C reveal molecular mechanisms for their dysfunction. Front Genet. 2023;14:1291307. PMCID: PMC10715303 |
42. Chi YI, Jorge SD, Jensen DR, Smith BC, Volkman BF, Mathison AJ, Lomberk G, Zimmermann MT, Urrutia R. A multi-layered computational structural genomics approach enhances domain-specific interpretation of Kleefstra syndrome variants in EHMT1. Comput Struct Biotechnol J. 2023;21:5249-5258. PMCID: PMC10632586 |
43. Kerber PJ, Nuñez R, Jensen DR, Zhou AL, Peterson FC, Hill RB, Volkman BF, Smith BC. Fragment-based screening by protein-detected NMR spectroscopy. Methods Enzymol. 2023;690:285-310. PMCID: PMC10657026 |
44. Bursch KL, Olp MD, Smith BC. Analysis of continuous enzyme kinetic data using ICEKAT. Methods Enzymol. 2023;690:109-129. PMCID: PMC10691744 |
45. Ratnasinghe BD, Haque N, Wagenknecht JB, Jensen DR, Valdivia Esparza GK, Leverence EN, Milech De Assuncao T, Mathison AJ, Lomberk G, Smith BC, Volkman BF, Urrutia R, Zimmermann MT. Beyond structural bioinformatics for genomics with dynamics characterization of an expanded KRAS mutational landscape. Comput Struct Biotechnol J. 2023;21:4790-4803. PMCID: PMC10570560 |
46. Fisher CL, Pavan M, Salmaso V, Keyes RF, Wan TC, Pradhan B, Gao ZG, Smith BC, Jacobson KA, Auchampach JA. Extrahelical Binding Site for a 1<i>H</i>-Imidazo[4,5-c]quinolin-4-amine A<sub>3</sub> Adenosine Receptor Positive Allosteric Modulator on Helix 8 and Distal Portions of Transmembrane Domains 1 and 7. Mol Pharmacol. 2024 Feb 15;105(3):213-223. PMCID: PMC10877738 |
47. Falco JA, Wynia-Smith SL, McCoy J, Smith BC, Weerapana E. Identification of Protein Targets of <i>S</i>-Nitroso-Coenzyme A-Mediated <i>S</i>-Nitrosation Using Chemoproteomics. ACS Chem Biol. 2024 Jan 19;19(1):193-207. PMCID: PMC11154738 |
48. Bursch KL, Goetz CJ, Jiao G, Nuñez R, Olp MD, Dhiman A, Khurana M, Zimmermann MT, Urrutia RA, Dykhuizen EC, Smith BC. Cancer-associated polybromo-1 bromodomain 4 missense variants variably impact bromodomain ligand binding and cell growth suppression. J Biol Chem. 2024 Apr;300(4):107146. PMCID: PMC11002309 |
49. Jones Lipinski RA, Stancill JS, Nuñez R, Wynia-Smith SL, Sprague DJ, Nord JA, Bird A, Corbett JA, Smith BC. Zinc-chelating BET bromodomain inhibitors equally target islet endocrine cell types. Am J Physiol Regul Integr Comp Physiol. 2024 Jun 01;326(6):R515-R527. PMCID: PMC11381023 |
50. Bursch KL, Goetz CJ, Smith BC. Current Trends in Sirtuin Activator and Inhibitor Development. Molecules. 2024 Mar 06;29(5). PMCID: PMC10934002 |
51. Pradhan B, Pavan M, Fisher CL, Salmaso V, Wan TC, Keyes RF, Rollison N, Suresh RR, Kumar TS, Gao ZG, Smith BC, Auchampach JA, Jacobson KA. Lipid Trolling to Optimize A<sub>3</sub> Adenosine Receptor-Positive Allosteric Modulators (PAMs). J Med Chem. 2024 Jul 25;67(14):12221-12247. PMCID: PMC11636968 |
52. Bohl K, Wynia-Smith SL, Jones Lipinski RA, Smith BC. Inhibition of Sirtuin Deacylase Activity by Peroxynitrite. Biochemistry. 2024 Oct 01;63(19):2463-2476. PMCID: PMC11524680 |
53. Zhang J, Chang J, Chen V, Beg MA, Huang W, Vick L, Wang Y, Zhang H, Yttre E, Gupta A, Castleberry M, Zhang Z, Dai W, Song S, Zhu J, Yang M, Brown AK, Xu Z, Ma YQ, Smith BC, Zielonka J, Traylor JG, Dhaou CB, Orr AW, Cui W, Zheng Z, Chen Y. Oxidized LDL regulates efferocytosis through the CD36-PKM2-mtROS pathway. bioRxiv. 2024 Jul 15. PMCID: PMC11275753 |
54. Nuñez R, Sidlowski PFW, Steen EA, Wynia-Smith SL, Sprague DJ, Keyes RF, Smith BC. The TRIM33 Bromodomain Recognizes Histone Lysine Lactylation. ACS Chem Biol. 2024 Dec 20;19(12):2418-2428. PMCID: PMC11706526 |
Editorials, Letters To Editor, Other |
1. Shteyer E, Smith B. Reply: To PMID 25479138. Gastroenterology. 2015 Jul;149(1):261-2. |
