CURRICULUM VITAE
David Harder PhD, MS
David Harder PhD, MS
Emeritus Professor
Department of Physiology
Department of Physiology
OFFICE ADDRESS: |
8701 Watertown Plank Road |
Milwaukee, WI 53226 |
EDUCATION: |
- 1972 B.S. Zoology, University of Wisconsin, Milwaukee, WI |
- 1975 M.S., Medical College of Wisconsin, Milwaukee, WI |
- 1976 PhD Biophysics, Medical College of Wisconsin, Milwaukee, WI |
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS: |
1977 - 1978 Postdoctoral Fellow, Department of Physiology, University of Virginia Medical School, Charlottesville, VA |
FACULTY APPOINTMENTS: |
09/1976 - 12/1976 Lecturer in Anatomy & Physiology, Dept. of Zoology, University of Wisconsin, Milwaukee, WI |
1976 - 1977 Research Associate, Medical College of Wisconsin, Milwaukee, WI |
1977 - 1978 Postdoctoral Fellow, Physiology, University of Virginia Medical School, P.O. Box 800793, Charlottesville, VA 22908 |
1978 - 1980 Assistant Professor of Physiology, College of Medicine, East Tennessee University, Johnson City, TN |
1980 - 1983 Research Assistant Professor of Physiology & Biophysics, University of Vermont, Burlington, VT |
1983 - 1987 Associate Professor, Department of Neurology, Medical College of Wisconsin, Milwaukee, WI |
1983 - 1987 Associate Professor, Department of Physiology, Medical College of Wisconsin, Milwaukee, WI |
1984 - 1987 Director, Neurology Research, Neurology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 |
1985 - Present Research Career Scientist, Veterans Administration Medical Center, Milwaukee, WI |
1987 - Present Professor of Physiology, Medical College of Wisconsin, Milwaukee, WI |
1992 - 2009 Director, Cardiovascular Research Center, Physiology, Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 |
1994 - Present Professor of Medicine, Medical College of Wisconsin, Milwaukee, WI |
2008 - 2015 Associate Dean for Research, Office of Research, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 |
2010 - 2015 Director for Mentoring, Clinical & Translational Science Institute, Education, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 |
2015 - 2017 Associate Dean for Mentoring, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 |
RESEARCH ADMINISTRATIVE APPOINTMENTS: |
1984 - 1987 Director of Neurology Research, Medical College of Wisconsin, Milwaukee, WI |
1992 - 2009 Director, Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI |
2008 - Present Associate Dean for Research, Medical College of Wisconsin, Milwaukee, WI |
2010 - Present Director for Mentoring, Clinical & Translational Science Institute, Medical College of Wisconsin, Milwaukee, WI |
AWARDS AND HONORS: |
1982 - 1987 American Heart Association Established Investigator Award |
1985 - Present Research Career Scientist Award, Veterans Administration |
01/1987 - Present Royal Society of Medicine, London |
06/1990 - Present Elected as a Fellow in the Council for High Blood Pressure Research and Council on Circulation, American Heart Association |
1990 - Present National Institutes of Health Method to Extend Research in Time (MERIT) Award |
12/1997 Nominated for the Bristol-Myers Squibb Award for Distinguished Achievement in Cardiovascular/Metabolic Research, University of British Columbia, Vancouver |
2000 Robert M. Berne Distinguished Lecturer Award, The American Physiological Society |
12/2003 Distinguished Service Award, Medical College of Wisconsin |
2007 Kohler Co. Endowed Chair in Cardiovascular Research, Medical College of Wisconsin |
2009 Carl I. Wiggers Award - Highest award presented for outstanding career contributions made in the field of pressure-flow relationships in cardiovascular system., Cardiovascular Section of American Society of Physiology |
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES: |
Society for General Physiologists |
Biophysics Society |
American Physiological Society |
International Society for Heart Research (American Section) |
Scientific Council of the American Heart Association (Circulation Fellow) |
EDITORSHIPS/EDITORIAL BOARDS/JOURNAL REVIEWS: |
Editorship |
1999 - 2004 American Journal of Physiology: Heart & Circulatory Physiology, Editor-in-Chief |
Journal of Vascular Research, Associate Editor |
Stroke, Guest Editor |
Editorial Board |
Circulation Research |
Endothelium |
Journal of Cerebral Blood Flow and Metabolism |
Journal of Vascular Research |
Journal Review |
Stroke |
American Journal of Physiology - all sections |
Arteriosclerosis and Thrombosis |
Cardiovascular Research |
Circulation Research |
Endothelium |
European Journal of Pharmacology |
Hypertension |
Journal of Cardiovascular Pharmacology |
Journal of Cerebral Blood Flow and Metabolism |
Journal of Clinical Investigation |
Journal of Vascular Research |
Pflugers Archives |
LOCAL/REGIONAL APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
1996 Chairman, First Annual Meeting, Midwest Physiological Society |
NATIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
1992 - 1995 Program Committee, Council for High Blood Pressure Research |
1994 - 1997 Committee on Committees, American Physiological Society |
1997 Finance Committee, American Physiological Society |
2002 - Present Publication Committee, Council for High Blood Pressure Research |
2004 - Present Finance Committee, World Congress for Microcirculation |
RESEARCH GRANTS/AWARDS/CONTRACTS/PROJECTS: | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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INVITED LECTURES/WORKSHOPS/PRESENTATIONS: |
International |
"Control of Excitation-Contraction Coupling and Contraction in Vascular Smooth Muscle", 5th International Congress on Vascular Neuroeffector Mechanisms, Paris, France, 05/1984 |
Erik K. Fernstrom Symposium on Neural Regulation of Brain Circulation: Effects of Neurotransmitters., Lund, Sweden, 06/1985 |
International Conference on Primary Pulmonary Hypertension, Sponsored by NIH/NHLBI, Philadelphia, Pennsylvania, 05/1987 |
Free University of Berlin, Berlin, Germany, 03/1989 |
International Conference on Myogenic Mechanisms, Maderia, Portugal, 03/1989 |
International Symposium on Endothelial Factors, Philadelphia, Pennsylvania, 04/1989 |
International Conference on Neurotransmission and Cerebrovascular Function, LaNapoule, France, 05/1989 |
International Heart Research Annual Meeting, Cincinnati, Ohio, 05/1991 |
Third International Symposium on Resistance Arteries, Denmark, 05/1991 |
Third International Symposium on Calcium and Gene Hypertension, Portland, Oregon, 07/1991 |
Sixth Grover Conference on Ion Channels in Smooth Muscle, Denver, Colorado, 10/1992 |
Tsumagoi Conference, Tokyo, Japan, 07/1994 - 01/1994 |
First Workshop on Endothelium-Derived Hyperpolarizing Factor, Paris, France, 06/1995 |
The University of British Columbia, Department of Pharmacology & Therapeutics, Vancouver, Canada, 01/1996 |
Satellite Symposium, Signal Transduction in the Endothelium, Vancouver, Canada, 09/1996 |
International Society for Heart Research (ISHR), The University of British Columbia, Vancouver, Canada, 07/1997 |
Symposium on signaling mechanisms in vascular smooth muscle, Sandbjerg, Denmark, 05/1998 |
Endothelium-Derived Hyperpolarizing Factor Workshop, Paris, France, 06/1998 |
Acta Physiological Scandinavica Symposium, Copenhagen, Denmark, 08/1999 |
11th International Conference on Advances in Prostaglandin and Leukotriene Research: Basic Science and New Clinical Applications, Florence, Italy, 06/2000 |
22nd meeting of the International Society for Heart Research (ISHR), Louisville, Kentucky, 06/2000 |
Cerebral vascular hyperpolarizing influences mechanistically inhibit blood flow autoregulation: a Yin-Yang hypothesis for hypothesis for cytochrome P450 metabolites, Third Workshop on Endothelium-Derived Hyperpolarizing Factor, Abbaye des Vaux de Cernay, France, 06/2000 |
XVII World Congress of International Society, Winnipeg, Canada, 07/2001 |
Invited Speaker, EDHF Workshop, Paris, France, 06/2002 |
Invited Speaker, 24th Annual Meeting of the ISHR, Madison, Wisconsin, 07/2002 |
Invited Speaker, XIV World Conference of Pharmacology, San Francisco, California, 07/2002 |
Invited Speaker, The University of British Columbia, Vancouver, B.C., 08/2002 |
Invited Speaker, ISHR, Mystic, Connecticut, 06/2003 |
Invited Speaker and Session Chair, 8th ISRA, Angers, France, 06/2004 |
Invited Speaker, International Society for Heart Research, North American Section, Toronto, Canada, 06/2006 |
Speaker, 5th Int’l EDHF Symposium, Tampere, Finland, 06/2008 |
Invited Co-Chair, Speaker, 11th Annual International Winter Eicosanoid Conference, 03/2009 |
Gebremedhin D, Zhang DX, Carver KA, Rau N, Roman RJ, Harder DR, Expression of CYP 4A-ω-hydroxylase and formation of 20-hydroxyeicosatetraenoic acid (20-HETE) in cultured rat brain astrocytes, 16th International Winter Eicosanoid Conference, Baltimore, Maryland, 03/13/2016 - 03/16/2016 |
National |
AHA Established Investigator Research Committee Meeting, Boston, Massachusetts, 02/1984 |
Uniformed Services University of the Health Sciences, Bethesda, Maryland, 03/1984 |
68th Annual Meeting of Federation of American Societies for Experimental Biology, St. Louis, Missouri, 04/1984 |
A.N. Richards Symposium, "Recent Advances in Arterial Disease"., Philadelphia Physiological Society, Philadelphia, Pennsylvania, 05/1984 |
Association of University Anesthetists Annual Meeting, Madison, Wisconsin, 05/1984 |
Conference on Pulmonary Vascular Reactivity, Dekkers, Colorado, 10/1984 |
University of Virginia, Department of Physiology, Charlottesville, Virginia, 03/1985 |
The Squibb Institute for Medical Research, Princeton, New Jersey, 05/1985 |
Symposium on smooth muscle, Federation of American Societies for Experimental Biology Research Conference, Sexton's River, Vermont, 06/1985 |
Cerebral Blood Flow Symposium, Federation of American Societies for Experimental Biology Annual Meeting, 04/1986 |
AHA 59th Scientific Sessions, Dallas, Texas, 11/1986 |
Berlex Pharmaceuticals, Cedar Grove, New Jersey, 10/1987 |
Summer Research Conference on Electrophysiological Mechanisms of Propagation and Activation of Cardiac and Smooth Muscle, Saxton's River, Vermont, 08/1988 |
The Graduate Hospital, Philadelphia, Pennsylvania, 10/1988 |
Dept. of Physiology, Texas A&M University, College of Medicine, 02/1990 |
FASEB Summer Research Conference on Renal Hemodynamics, Saxtons River, Vermont, 06/1992 |
American Nephrology Society, Baltimore, MD, 11/1992 |
Bowman Gray University, Department of Physiology, Winston Salem, North Carolina, 05/1993 |
FASEB Summer Research Conference, Cooper Mt., Colorado, 06/1994 |
University of Louisville, Coll. of Medicine, Dept. of Physiology, Louisville, Kentucky, 11/1994 |
Symposium on New Methodologies in Cerebral Circulation, Experimental Biology, 04/1995 |
FASEB Summer Research Conference, Saxtons River, Vermont, 06/1995 |
Johns Hopkins University School of Medicine, Department of Anesthesiology & Critical Care Medicine, Baltimore, Maryland, 01/1996 |
Gordon Research Conferences, Ventura, California, 02/1996 |
The University of Texas Health Science Center at San Antonio, Department of Physiology, San Antonio, Texas, 03/1996 |
Experimental Biology, Washington, D.C., 04/1996 |
Yale University, The John B. Pierce Laboratory, Center for Research in Health and the Environment, New Haven, Connecticut, 12/1996 |
Vascular Biology, New Orleans, Louisiana, 04/1997 |
FASEB Summer Conference on Endothelium and Cardiovascular Control, Copper Mountain, Colorado, 06/1997 |
Brain Satellite Symposium, Williamsburg, Virginia, 06/1997 |
University of Nevada-Reno, Department of Physiology, Reno, Nevada, 12/1997 |
Vascular and Myocardial Aspects of Ischemic Heart Disease, Incline Village, Nevada, 02/1998 |
University of Tennessee-Memphis, Dept of Physiology & Biophysics, 04/1998 |
Experimental Biology, San Francisco, California, 04/1998 |
Vascular Biology, San Francisco, California, 04/1998 |
Virginia Commonwealth University, Dept. of Pharmacology, Richmond, Virginia, 05/1998 |
Renal Hemodynamics: Integration of Endothelial, Epithelial, and Vascular Control Mechanisms, FASEB Summer Research Conference, Saxtons River, Vermont, 06/1998 |
52nd Annual Fall Conference and Scientific Sessions of the Council for High Blood Pressure Research, Philadelphia, Pennsylvania, 09/1998 |
American Heart Association, 71st Scientific Sessions, Dallas, Texas, 11/1998 |
Winter Eicosanoid Conference, Baltimore, Maryland, 03/1999 |
Experimental Biology, Washington, DC, 04/1999 |
Vasoactive Substances Conference, Alexandria, Virginia, 05/1999 |
53rd Annual Council for High Blood Pressure Research, Orlando, Florida, 09/1999 |
Winter Conference on Brain Research, Breckenridge, Colorado, 01/2000 |
Winter Eicosanoid Conference, Baltimore, Maryland, 03/2000 |
Robert M. Berne Distinguished Lecturer of the APS Cardiovascular Section, Experimental Biology, San Diego, CA, 04/2000 |
Invited Speaker, New York Medical College, Valhalla, New York, 04/2001 |
Experimental Biology, Orlando, Florida, 04/2001 |
Speaker, Mechanisms of Vasodilation Symposium, Boston, Massachusetts, 05/2001 |
Speaker, Mechanisms of Vasodilation Symposium, Boston, Massachusetts, 05/2001 |
Speaker, University of Mississippi Seminar, Jackson, Mississippi, 10/2001 |
Winter Brain Conference, Aspen, Colorado (Snowmass Village), 01/2002 |
Speaker, Medical College of Georgia-Seminar, Augusta, Georgia, 02/2002 |
Winter Eicosanoid Conference, Baltimore, Maryland, 03/2002 |
Invited Speaker, Myogenic Centennial Conference, Stowe, VT, 06/2002 |
Winter Eicosanoid Conference, Baltimore, Maryland, 03/2003 |
Invited Speaker, Symposium on Eicosanoids and Cardiovascular Disease in honor of Dr. John McGiff, New York Medical College, Valhalla, New York, 05/2003 |
Moderator and presenter, 57th Annual Fall Conference for American Heart Association, Washington, DC, 09/2003 |
Winter Eicosanoid Conference, Baltimore, Maryland, 03/2004 |
Invited Speaker, Louisiana State University, New Orleans, Louisiana, 04/2004 |
Experimental Biology Conference, Washington, DC, 04/2004 |
Invited Speaker, Wake Forest University, Winston-Salem, North Carolina, 05/2004 |
Invited Speaker, Gordon Research Conference, Colby College, Waterville, Maine, 08/2004 |
Invited Speaker, Brain Uptake and Utilization of Fatty Acids, Lipids & Lipoproteins Conference, Bethesda, Maryland, 10/2004 |
Symposium Co-chair, Experimental Biology Conference, San Diego, California, 03/2005 |
Symposium Co-chair, 7th Annual Winter Eicosanoid Conference, Baltimore, Maryland, 03/2005 |
American Heart Association’s Scientific Sessions, Dallas, Texas, 11/2005 |
Invited Speaker, University of California, Los Angeles, CA, 02/2006 |
Symposium Co-chair, 8th Annual Winter Eicosanoid Conference, Baltimore, Maryland, 03/2006 |
Presenter, 4th Scientific Meeting of the TMJ Association, Bethesda, Maryland, 09/2006 |
Invited Speaker, Institute for Environmental Medicine, University of Pennsylvania Medical Center, 12/2006 |
Invited Speaker, Loma Linda University, Loma Linda, California, 02/2007 |
Symposium Co-chair and Presenter, 9th Annual Winter Eicosanoid Conference, Baltimore, Maryland, 03/2007 |
Invited Speaker, National Institute on Drug Abuse, Bethesda, MD, 05/2007 |
APS Cardiovascular Section 2009 Carl J. Wiggers Award, Experimental Biology, New Orleans, Louisiana, 04/2009 |
Speaker, Ion Channel Conference, Boston, MA, 10/2009 |
Winter Eicosanoid Conference, Baltimore, Maryland, 03/2011 |
Experimental Biology Conference, Washington, DC, 04/2011 |
Gebremedhin D, Zhang DX, Harder DR, Detection of TRPV4 channel current-like activity in Fawn Hooded rat (FHH) rat cerebral arterial muscle, Experimental Biology, San Diego, California, 04/02/2016 - 04/06/2016 |
Regional |
University of Michigan Medical School, Department of Physiology, Ann Arbor, MI, 12/1984 |
Reversal of cerebral vasospasm in the primate, Upjohn Pharmaceuticals, Kalamazoo, Michigan, 06/1986 |
Department of Physiology Seminar, University of Cincinnati, 09/1987 |
The University of Miami, 12/1988 |
Department of Pathology, University of Chicago, 04/1991 |
University of Iowa, College of Medicine, Department of Medicine, Iowa City, IA, 08/1994 |
University of Missouri, Dalton Cardiovascular Research Center, Colombia, MO, 02/1998 |
Role of P450 Eicosanoids in Brain Angiogenesis and Functional Hyperemia, Cardiovascular Institute Seminar, Loyola University Medical Center, Maywood, Illinois, 05/2000 |
Physiology Invited Speaker, University of Missouri, Columbia, Missouri,, 02/2001 |
Speaker, University of Illinois Seminar, Chicago, Illinois, 01/2002 |
Invited Speaker, University of Indiana, Indianapolis, IN, 10/2003 |
Symposium honoring Dr. Erdos/Endowment Fund, University of Chicago, Illinois, 10/2005 |
Invited Speaker, Loyola University, Chicago, IL, 12/2007 |
Local |
Invited Speaker, Stroke on the Move in Wisconsin Conference, Wisconsin Stroke Committee, Middleton, Wisconsin, 11/2005 - 11/2055 |
Metabolic Syndrome Symposium,, Milwaukee, WI, 02/2011 |
COMMITTEE SERVICE: |
Medical College of Wisconsin |
1984 - 1987 Executive Committee, Department of Neurology |
1985 - 1987 Chairman, Education Committee, Department of Neurology |
1987 Neurobiology Director Search Committee |
1990 - 1994 Rank and Tenure Committee |
1991 Chairman, Cardiovascular Research Center Executive Committee |
1992 - 1994 Basic Science Chair Committee |
1992 - Present Strategic Research and Planning Committee |
1997 - Present Clinical Research Center Advisory Board |
1998 Cardiology Chair Search Committee |
1999 - Present Research Space Advisory Board |
2000 - Present Cardiovascular Strategy Committee |
2000 - Present Budget Committee |
2000 - Present Cardiovascular Board Development Committee |
2000 - 2003 Technology Transfer Committee |
2002 - Present Angiographic Suite Committee |
2002 - Present Translational Medicine Committee |
2002 - Present Executive Committee of the Faculty |
2009 Grants and Awards Committee, MCW Foundation |
MCW TEACHING ACTIVITIES: |
Medical Student Education |
1983 - 1995 CNS physiology lectures to medical students in Dept. of Physiology |
Graduate Student Education |
1983 - 1995 Graduate courses in cell physiology. Approximately 32 contact hours/year. Graduate courses in cell physiology and signal transduction. |
2010 - Present Special course in NIH Grant Preparation “Methods in Grant Preparation” (Syllabus can be found on www.ctsi.mcw.edu ) |
Continuing Medical Education |
2008 - Present Associate Dean for Research: the primary responsibility of this post is to mentor research intensive clinical faculty in the writing and prosecution of extramural grants, including VA Merit Review and Clinician investigator awards. |
EXTRAMURAL TEACHING: |
Medical Student Education |
1978 - 1980 East Tennessee State University, Dept. of Physiology, Taught 25% of medical physiology lectures plus labs. (This was a new medical school.) Duties included developing curriculum, teaching labs, and course implementation for medical students. |
MCW STUDENTS, FACULTY, RESIDENTS AND CLINICAL/RESEARCH FELLOWS MENTORED: |
Graduate Students |
PhD Students Advised |
1996 Nabil Alkayed, Medical College of Wisconsin |
1997 Andrew Lang, Medical College of Wisconsin |
2009 Lucas Hart, Medical College of Wisconsin |
2012 Pallabi Sarkar, Medical College of Wisconsin |
Students Advised |
2002 Reza Taheri, Medical College of Wisconsin |
Koryn Carver, Medical College of Wisconsin |
Postdoctoral Researchers |
C.F. Sanchez-Ferrer, M.D., Ph.D, Advisor, Medical College of Wisconsin |
P. Bonnet, M.D., Ph.D, Advisor, Medical College of Wisconsin |
D. Gebremedhin, Ph.D, Advisor, Medical College of Wisconsin |
Dr. Yunn-Ha Ma, Ph.D., Advisor, Medical College of Wisconsin |
Dr. K. Kauser, M.D., Advisor, Medical College of Wisconsin |
A. Hudetz, Ph.D, Advisor, Medical College of Wisconsin |
C. Cheliakine, M.D., Advisor, Medical College of Wisconsin |
P. Li, M.D., Advisor, Medical College of Wisconsin |
W. Meng, Ph.D., Advisor, Medical College of Wisconsin |
E. Birks, DVM, Ph.D., Advisor, Medical College of Wisconsin |
N. Dovin, M.D., Advisor, Medical College of Wisconsin |
Meetha Medhora, Ph.D., Advisor, Medical College of Wisconsin |
Diane Munzenmaier, Ph.D., Advisor, Medical College of Wisconsin |
Drazen Zagorac, Advisor, Medical College of Wisconsin |
Johan Bylund, Advisor, Medical College of Wisconsin |
Ken Yamaura, Advisor, Medical College of Wisconsin |
Danica Jakovcevic, MD., Advisor, Medical College of Wisconsin |
Chenyang Zhang, Advisor, Medical College of Wisconsin |
Nadi Wickramasakera, Ph.D., Advisor, Medical College of Wisconsin |
Vidudala Prasad, Ph.D., Advisor, Medical College of Wisconsin |
Maia Terashvili, Ph.D., Advisor, Medical College of Wisconsin |
David Lourim, Ph. D., Advisor, Medical College of Wisconsin |
U. Wesselmann, M.D., Ph.D., Advisor, Medical College of Wisconsin |
J. Madden, Ph.D., Advisor, Medical College of Wisconsin |
P. Dernback, M.D, Advisor, Medical College of Wisconsin |
R. Gilbert, M.D, Advisor, Medical College of Wisconsin |
A. Waters, M.D., Advisor, Medical College of Wisconsin |
J. Smeda, Ph.D, Advisor, Medical College of Wisconsin |
ADVISORY ACTIVITIES / GRANT REVIEWS:: |
1989 - 1993 National Institutes of Health; National Heart, Lung and Blood Institute, Bethesda, MD: Experimental Cardiovascular Sciences Study Section, Ad-Hoc |
1989 - 1993 National Institutes of Health; National Heart, Lung and Blood Institute, Bethesda, MD: Clinical Sciences Study Section |
1992 NIH Site Visit, Program Projects in Vascular Biology |
1994 NIH delegate to Japan |
1996 NIH, RFA Study Section on Mechanisms of Angiogenesis |
1996 - 1999 National American Heart Association Molecular Signaling Study Section |
2000 - Present NIH Experimental Cardiovascular Sciences (ECS) Study Section |
CONSULTANTSHIPS: |
Uniformed Services University, Department of Physiology, Bethesda, Maryland |
Squibb Institute for Medical Research, Upjohn Pharmaceuticals, Kalamazoo, Michigan
|
Cytometix, Inc. - Cytometix, Inc., was founded in the Fall of 2004 as a partnership between TechStar and David Harder, Director of the Cardiovascular Center (CVC) at the Medical College of Wisconsin (MCW). Cytometix is a privately funded, emerging pharmaceutical company focused on pre-clinical development of a lipid compound for pain. Cytometix was awarded an SBIR grant to validate the prospective use of this analgesic therapy from the National Institute on Drug Abuse (NIDA). Cytometix is also in early development of a therapeutic for asthma. The company has obtained over $8million dollars in Private Investment Capital (Angel Investments). An IND Application from FDA is currently pending. We anticipate initiation of Phase 1 Clinical Trials beginning November 1 2012. |
Berlex Laboratories, Inc., University of Louisville, Department of Physiology
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RESEARCH INTERESTS: |
Mechanisms Controlling Autoregulation of Blood Flow |
Role of Ionic Mechanisms in Activating Arterial and Arteriolar Smooth Muscle
|
Tumor Angiogenesis in Brain Pain – Behavioral Study |
Electrophysiology of Excitable Tissues
-Ion channels
-Voltage gated mechanisms
-Receptor operated mechanisms
|
Control of Renal Blood Flow |
Cytochrome P450 Metabolites in the Control of Vascular Smooth Muscle and Endothelium |
Biochemistry and Molecular Biology of Membrane Lipids in Vascular Muscle, Neurons and Their Effect on Ion Channels |
Endothelial Dependent Mechanisms in Vascular Smooth Muscle |
Mechanisms Responsible for Myogenic Activation of Arteries
|
Cellular Mechanisms Controlling Cerebral Blood Flow |
RESEARCH TECHNIQUES USED IN THE LABORATORY:: |
Intracellular electrophysiology of vascular muscle and glial cells |
Measurement of intracellular ions using fluorescent probes |
Patch clamp analysis of ion channels |
In-vitro techniques for measurement of muscle cell activation |
Development of molecular reagents:
- antibody production
- PCR primers
- oligonucleotides
- DNA libraries
|
Molecular biology
- Western blots
- Northern blots
- RT PCR
- DNA sequencing
- cloning
- in-situ hybrydization |
Protein chemistry |
Analytical chemistry (i.e. separation of fatty acid metabolites) |
Immunohistochemistry |
In-vivo measurement of cerebral and other organ blood flow |
Confocal microscopy |
Gas chromatography / mass spectrophotometry |
BIBLIOGRAPHY |
Refereed Journal Publications/Original Papers |
1. Harder DR, Sperelakis N (1978) Membrane electrical properties of vascular smooth muscle from guinea-pig superior mesenteric artery. Pflugers Arch 378: 111- 119 |
2. Harder DR, Sperelakis N (1979) Action potential generation in reaggregates of rat aortic smooth muscle cells in primary culture. Blood Vessels 16:186-201 |
3. Harder DR, Belardinelli L, Sperelakis N, Rubio R, Berne RM (1979) Differential effects of adenosine and nitroglycerine on the action potentials of large and small coronary arteries. Circ Res 44:176-182 |
4. Harder DR, Sperelakis N (1979) Action potentials induced in guinea-pig arterial smooth muscle by tetraethylammonium. Am J Physiol 237:C75-C80 |
5. Belardinelli L, Harder DR, Sperelakis N, Rubio R (1979) Cardiac glycoside stimulation of inward Ca2+ current in vascular smooth muscle of canine coronary artery. J Pharm Exp Ther 209:62-66 |
6. Harder DR, Coulson PB (1979) Estrogen receptors and effects of estrogens on membrane electrical properties of coronary vascular smooth muscle. J Cell Physiol 100:375-382 |
7. Berne RM, Belardinelli L, Harder DR, Sperelakis N, Rubio R (1980) Response of large and small coronary arteries to adenosine, nitroglycerine, cardiac glycosides and calcium agonists. In: Calcium Antagonisms (A Fleckenstein & H Roskem, eds) Springer-Verlag, Berlin pp: 208-220 |
8. Harder DR (1980) Membrane electrical effects of histamine on vascular smooth muscle of canine coronary artery. Circ Res 46:372-377 |
9. Stekiel WJ, Willems WJ, Harder DR, Lombard JH (1980) Active vascular smooth muscle tone and venous membrane potentials during hemorrhage. Am J Physiol 238:H144-H152 |
10. Wondergem R, Harder DR (1980) Membrane potential measurements during rat liver regeneration. J Cell Physiol 102:193-197 |
11. Harder DR (1980) Comparison of electrical properties of middle cerebral and mesenteric arteries in cat. Am J Physiol 239:C23-C26 |
12. Wondergem R, Harder DR (1980) Transmembrane potential and amino acid transport in rat hepatocytes in primary culture. J Cell Physiol 104:53-60 |
13. Harder DR, Belardinelli L (1980) Effects of propafenone on TEA-induced action potentials in vascular smooth muscle of canine coronary artery. Experientia 36:1082-1083 |
14. Harder DR, Contney SJ, Willems WJ, Stekiel WJ (1981) Norepinephrine effect on in situ venous membrane potentials in spontaneously hypertensive rats. Am J Physiol 240:H837-H842 |
15. Harder DR, Sperelakis N (1981) Bebridil blockade of Ca-dependent action potentials in vascular smooth muscle of dog coronary artery. J Cardiovascular Pharm 3:906-914 |
16. Harder DR (1981) Membrane activation of smooth muscle from rabbit basilar artery by dopamine. Pflugers Arch 390:296-298 |
17. Harder DR, Abel PW, Hermsmeyer K (1981) Membrane electrical mechanism of basilar artery constriction and pial artery dilation by norepinephrine. Circ Res 49:1237-1242 |
18. Harder DR (1982) Membrane electrical activation of arterial smooth muscle. In: Vascular Smooth Muscle. (M Crass & CD Barnes, eds) Academic Press, New York pp. 71-97 |
19. Hermsmeyer K, Abel PW, Harder DR (1982) Membrane electrical responses to histamine in muscle cells of cat middle cerebral artery. In: Cerebral Blood Flow: Effect of Nerves and Neurotransmitters. (D Heistad & ML Marcus, eds) Elsevier, New York. pp. 3-12 |
20. Harder DR (1982) Effect of H+ and elevated PCO2 on membrane electrical properties of cerebral arteries. Pflugers Arch 394:182-185 |
21. Willems WJ, Harder DR, Contney SJ, McCubbin JW, Stekiel WJ (1982) Sympathetic supraspinal control of venous membrane potential in spontaneous hypertension in vitro. Am J Physiol 243:C101-C106 |
22. Harder DR, Stekiel WJ, Lombard JH (1982) Effect of methylprednisolone on small vein responses to hemorrhage and norepinephrine. Eur J Pharm 81: 163-16 |
23. Pamnani MB, Harder DR, Hout SJ, Bryant HJ, Kutyna FJ, Haddy FJ (1982) Vascular smooth muscle membrane potential and a ouabain-like humoral factor in one-kidney, one- clip hypertension in rats. Clinical Sci 63:31-33 |
24. Harder DR, Brann L, Halpern W (1983) Altered membrane electrical properties in smooth muscle cells of small cerebral arteries from hypertensive rats. Blood Vessels 20: 154- 160 |
25. Harder DR (1983) Heterogenicity of membrane properties in vascular muscle cells from various vascular beds. Fed Proc 42:253-256. |
26. Schmidek HH, Moreira D, Harder DR (1983) Diatrizoate meglumine (renografin) induced dilation of rat basilar artery: in in vitro study. J Neurosurgery 12:137-141 |
27. Hout S, Pamnani M. Clough D, Buggy J, Bryant HJ, Harder DR, Haddy FJ (1983) Na-K pump in reduced renal mass hypertension. Hypertension 5:94-101 |
28. Harder DR, Hermsmeyer K (1983) Membrane mechanisms in arterial hypertension. Hypertension 5:404-408 |
29. Harder DR, Waters A (1983) Electromechanical coupling in feline basilar artery in response to serotonin. Eur J Pharm 93:95-100 |
30. Stekiel WJ, Contney SJ, Lombard JH, Harder DR (1983) Responses of venous membrane potentials to calcium blockade in spontaneous hypertension. Hypertension 5:64-70 |
31. Waters A, Harder DR (1983) Electromechanical coupling in rat basilar artery in response to morphine. Neurosurgery 13: 676-680 |
32. Waters A, Harder DR (1983) Dexamethasone-induced hypersensitivity to serotonin in feline cerebral-arteries. Stroke 14: (1) 126. |
33. Harder DR, Waters A (1984) Electrical activation of arterial muscle. International Review of Cytology. Vol 89 (GH Bourne & JF Danielli, eds) Academic Press, Florida. pp. 137- 149 |
34. Harder DR, Madden JA (1984) Cellular mechanisms of opiate receptor stimulation in cat middle cerebral artery. Eur J Pharm 102:411-416 |
35. Harder DR (1984) Voltage-dependent mechanisms of receptor stimulation in cerebral arterial muscle. Blood Vessels 21: (4) 189-190 |
36. Harder DR (1984) Pressure-dependent membrane depolarization in cat middle cerebral artery. Circ Res 55:197-202 |
37. Harder DR, Madden JA (1985) Cellular mechanisms of force development in cat middle cerebral artery by reduced PCO2. Pflugers Arch 403:402-406 |
38. Harder DR, Lombard JH (1985) Voltage dependent mechanisms of receptor stimulation in cerebral arterial muscle. Vascular Neuroeffector Mechanisms (J Beven et al., eds) Elsevier, Netherlands. pp. l81-186 |
39. Gilbert RJ, Sarna SK, Harder DR (1985) Effect of morphine on intracellular electrophysiological characteristics of circular and longitudinal smooth-muscle. Digest Dis Sci 30: (8)770. |
40. Madden JA, Dawson CA, Harder DR (1985) Hypoxia-induced activation in small isolated pulmonary arteries of the cat. J Appl Physiol 59(1): 113-118 |
41. Bryant JH, Harder DR, Pamnani MB, Haddy FJ (1985) In vivo membrane potentials of smooth muscle cells in the caudal artery of the rat. Am J Physiol 249:C78-C83 |
42. Harder DR (1985) A cellular mechanism for myogenic regulation of cat cerebral arteries. Annals of Biomedical Eng 13:335-339 |
43. Harder DR, Gradall K, Madden JA, Kampine JP (1985) Cellular actions of halothane on cat cerebral arterial muscle. Stroke 16:680-683 |
44. Waters A, Harder DR (1985) Altered membrane properties of cerebral vascular smooth muscle following subarachnoid hemorrhage: an electrophysiological study.I. Changes in resting membrane potential (Em) and effect on the electrogenic pump potential contribution to Em. Stroke 16(6): 990-997 |
45. Pamnani MB, Bryant HJ, Harder DR, et al (1985) Vascular smooth-muscle membrane-potentials in rats with one-kidney, one clip and reduced renal mass-saline hypertension-the influence of a humoral sodium-pump inhibitor. J Hypertens 3: S29-S31. |
46. Harder DR, Madden JA, Dawson CA (1985) A membrane electrical mechanism for hypoxic vasoconstriction of small pulmonary arteries from cat. Chest 88:233S-235S |
47. Harder DR, Madden JA, Dawson CA (1985) Hypoxic induction of Ca2+ dependent action potentials in small pulmonary arteries of the cat. J Appl Physiol 59:1389-1393 |
48. Harder DR, Smeda J, Lombard JH (1985) Enhanced myogenic depolarization in hypertensive cerebral arterial muscle. Circ Res 57:319-322 |
49. Hermsmeyer K, Harder DR (1986) Membrane ATPase mechanisms of the K+ return relaxation in stroke-prone SHR and WKY arterial muscle. Am J Physiol 250:C557-C562 |
50. Leo GJ, Konkol RJ, Harder DR, et al (1986) Vascular function and innervation in experimental allergic encephalomyelitis. Can J Neurol Sci 13: (4) 378. |
51. Lombard JH, Willems WJ, Harder DR, Stekiel WJ (1986) Vascular smooth muscle transmembrane potentials during hypotensive stress. Circulatory Shock 18:131-140 |
52. Harder DR (1986) Electrophysiological mechanisms of arterial muscle activation: alterations associated with cerebral vasospasm and hypertension. In: Recent Advances in Arterial disease: Atherosclerosis, Hypertension and Vasospasm. Alan R. Liss, Inc. pp. 245-263 |
53. Gilbert RJ, Sarna SK, Harder DR (1986) Regional electrical and mechanical-properties of circular muscle of the small-intestine. Gastroenterology 90: (5) 1427. |
54. Harder DR, Madden JA (1986) Membrane ionic mechanisms controlling activation of cerebral arterial muscle. In: Neural Regulation of Brain Circulation (C Owman & JE Hardebo, eds). Eric K. Fernstrom Symposium on Neural Regulation of Brain Circulation: Effects of Neurotransmitters (Lund, Sweden). Elsevier, Netherlands. pp. 81-91 |
55. Lombard JH, Stekiel WJ, Harder DR (1986) Membrane potentials in vascular smooth muscle of microvessels. In: Micro-circulatory Techniques -Physical Techniques in Biology and Medicine (CH Baker & W Nastuk, eds) Academic Press, New York. pp. 299-315 |
56. Gilbert RJ, Sarna SK, Harder DR (1986) Regional differences in excitation-contraction (EC) coupling in the circular muscle of the small-intestine. Gastroenterology 91: (4) 1053. |
57. Harder DR (1986) Cellular mechanism of cerebral vasospasm following subarachnoid hemorrhage and its reversal by nicorandil. Circulation 74: (4) 155 Part 2 Suppl. |
58. Lombard JH, Smeda J, Madden JA, Harder DR (1986) Effect of reduced oxygen availability upon myogenic depolarization and contraction of cat middle cerebral artery. Circ Res 58:565-569 |
59. Smeda JS, Lombard JH, Madden JA, Harder DR (1987) The effect of alkaline pH and transmural pressure on arterial constriction and membrane potential of hypertensive cerebral arteries. Pflugers Archiv 408:239-242 |
60. Gilbert RJ, Sarna SK, Harder DR (1987) Effect of morphine on electrophysiological properties of circular and longitudinal muscle. Am J Physiol 252:G333-G338 |
61. Harder DR (1987) Pressure-induced myogenic activation of cat cerebral arteries is dependent on intact endothelium. Circ Res 60:102-107 |
62. Wesselmann U, Konkol RJ, Leo GL, Roerig DL, Harder DR (1987) Altered splenic catecholamine concentrations during experimental allergic encephalomyelitis. Pharmacol Biochem Behav 26(4):851-854 |
63. Harder DR, Dernbach P, Waters A (1987) A possible cellular mechanism for cerebral vasospasm following experimental subarachnoid hemorrhage in the dog. J Clin Invest 80:875-880 |
64. Madden JA, Kalbfleisch JH, Harder DR (1987) Distribution of excitatory junction potential amplitude in cat cerebral arteries: examination of its quantal nature and modulation by opiates. J Cell Physiol 131:262-266 |
65. Harder DR, Madden JA (1987) Electrical stimulation of the endothelial surface of pressurized cat middle cerebral artery results in TTX-sensitive vasoconstriction. Circ Res 60:831-836 |
66. Harder DR, Gilbert R, Lombard JH (1987) Vascular smooth muscle cell depolarization and activation in renal arteries on elevation of transmural pressure. Am J Physiol 253:F778-F781 |
67. Wesselmann U, Konkol RJ, Leo G, Roerig DL, Harder DR (1987) Neurochemical and histochemical changes in the autonomic nervous systems of rats with experimental allergic encephalomyelitis. Biochem Pharmacol Behav 26:851-854 |
68. Harder DR (1988) Increased sensitivity of cat cerebral arteries to serotonin upon elevations of transmural pressure. Pflugers Archiv 411: 698-700 |
69. Wesselmann U, Konkol RJ, Roerig DL, Harder DR (1988) Early effects of experimental diabetes on central catecholamine concentrations. Brain Res Bul 20:651-654 |
70. Sanchez-Ferrer CF, Lombard JH, Roman RJ, Harder DR (1988) Mechanism of pressure-dependent activation in renal arteries and arterioles. In: Proceedings of the 2nd International Symposium on Resistance Arteries (Halpern, W. et al., eds) Perinatology Press, Ithaca, New York, pp. 369-377 |
71. Lombard JH, Harder DR, Stekiel WJ (1988) Hypoxic vasodilation: Hypertension. In: Mechanisms of vasodilation (PM Vanhoutte, ed) Raven Press. pp 61-65. |
72. Harder DR, Sanchez-Ferrer C, Roman RJ (1988) Possible myogenic auto-regulation of juxtamedullary glomerular blood-flow by afferent arterioles. Blood Vessels 25: (1) 36. |
73. Kauser K, Stekiel WJ, Rubanyi GM, Harder DR (1989) Mechanism of action of EDRF on pressurized arteries: effect on K+ conductance. Circ. Res. 65:199-204 |
74. Harder DR, Sanchez-Ferrer CF, Kauser K, Stekiel WJ, Rubanyi GM (1989) Pressure releases a transferable endothelial contractile factor in cat cerebral arteries. Circ. Res. 65:193-198 |
75. Sanchez-Ferrer CF, Roman RJ, Harder DR (1989) Pressure-dependent contraction of rat juxtamedullary afferent arterioles. Circ Res 64:790-798 |
76. Cowley AW, Hinojosa-Laborde C, Barber BJ, Harder DR, Lombard JH, Greene AS (1989) Short-term autoregulation of systemic blood flow and cardiac output. News In Physiol Sciences 4: 219-225 |
77. Harder DR, Kauser K, Roman R.R., Lombard JH (1989) Mechanisms of pressure-induced myogenic activation of cerebral and renal arteries: Role of the endothelium. J. of Hypertension 7: S11-S15 |
78. Kauser K, Rubanyi G.M., Roman R.J., Harder, DR (1989) Pressure-induced endothelium-dependent contraction in cat cerebral arteries. In: Neurotransmission and Cerebrovascular Function (J. Seylaz and E.T. MacKenzie, eds.) Excerpta Medica, New York pp. 71-76 |
79. Harder DR (1989) Electrophysiological Responses of Cerebral Arterial Muscle to Environmental Stimuli Symposium on Neural Transmission and Cerebral Blood Flow. (J. Seylaz and R. Sercombe, eds.) Excerpta Medica, New York pp. 51-63 |
80. Kauser K, Rubanyi G, Harder DR, (1989) Endothelium dependent modulation of endothelin-induced vasoconstriction and membrane depolarization in cat cerebral arteries. J. Pharm. Exp. Ther. 252: 93-97 |
81. Lombard JH, Eskinder H, Kauser K, Osborn JL, Harder DR (1990) Enhanced norepinephrine sensitivity in renal arteries at elevated transmural pressure. Am. J. Physiol.259 (Heart Circ 28): H29-H33 |
82. Eskinder H, Harder DR, Lombard JH (1990) Role of the vascular endothelium in regulating the response of small arteries of the dog kidney to transmural pressure elevation and reduced PO2. Circ. Res. 66:1427-1435 |
83. Gebremedhin D, Fenoy FJ, Harder DR, Roman RJ (1990) Enhanced vascular tone in the renal vasculature of spontaneously hypertensive rats. Hypertension 16:648-654 |
84. Rubanyi GM, Freay AD, Kauser K, Johns A, Harder DR (1990) Mechanoreception by the endothelium: mediators and mechanisms of pressure- and flow-induced vascular responses. Blood Vessels 27: 246-257 |
85. Harder DR, Kauser JH, Lombard JH, Roman RJ, Rubanyi G. (1990) Pressure-induced activation of renal and cerebral arteries depends upon an intact endothelium. In: Endothelium-Derived Contracting Factors. Vol. 2 (GM Rubanyi, P Vanhoutte, eds). Basel: Karger, pp 8-13 |
86. Lougee L, Hinojosa-Laborde C, Harder DR, Lombard JH (1990) Effect of nifedipine on endothelin induced contractions of skeletal muscle arterioles of spontaneously hypertensive rats. Microcirc Endoth Lymphatics 6:355-368 |
87. Harder DR, Roman RJ, Ma Y-H, Clark JE, Masters BS, Van Rollins M, Gebremedhin D. (1990) Regulation of vascular muscle K+ conductance by-products of cytochrome P450 metabolism. In: Proceedings of The Third International Symposium on Resistance Arteries, Denmark. |
88. Bonnet P, Gebremedhin D, Rusch NJ, Harder DR (1991) Effects of hypoxia on a potassium channel in cat cerebral arterial muscle cells. Z Kardiol 80 (Suppl 7):25-27 |
89. Subramanian M, Madden JA, Harder DR (1991) A Method for the isolation of cells from arteries of various sizes. J. Tiss. Cult. Meth. 13: 13-20 |
90. Kauser K, Clark JE, Masters B.S.S., Ortiz de Montellano PR, Ma Y-H, Harder DR, Roman RJ (1991) Inhibitors of cytochrome P-450 attenuate the myogenic response of dog renal arcuate arteries. Circ. Res. 68: 1154-1163 |
91. Harder DR, Roman RJ, Lombard JH. (1991) Cellular and biochemical mechanisms of pressure-induced myogenic tone in arteries. In: Resistance Arteries, Structure and Function: Excerpta Medica. (MJ Mulvany, ed). Elsevier Science Publishers, Amsterdam, pp. 178-182 |
92. Bonnet P, Rusch NJ, Harder DR (1991) Characterization of an outward K+ current in freshly dispersed cerebral arterial muscle cells. Pflugers Archiv 418:292-296 |
93. Ma YH, Harder DR, Clark SE, Roman RJ (1991) Effects of 12-HETE on isolated dog renal arcuate arteries. Am. J. Physiol. 261 (Heart Circ Physiol 30): H451-H456 |
94. Al-Tinawi A, Madden A, Dawson CA, Linehan JH, Harder DR, Rickaby DA (1991) Distensibility of small arteries of the dog lung. J. Applied Physiol. 71:1714-1722 |
95. Masters BSS, Clark JE, Roman LJ, McCabe TJ, Helm CB, Johnson EF, Ma Y-H, Harder DR, Roman RJ (1991) Structure-function studies and physiological roles of eicosanoids metabolized by cytochrome P450 w-Hydroxylases. In: Prostaglandins, Leukotrienes, Lipoxins and PAF. Plenum Publishing Co., New York. |
96. Al-Tinawi A, Clough AV, Harder DR, Linehan JH, Rickaby DA, Dawson CA. (1991) Distensibility of small veins of the dog lung. J Appl Physiol 73:2158-2165 |
97. Hudetz AG, Roman RJ, Harder DR (1992) Spontaneous flow oscillations in the cerebral cortex during acute changes in mean arterial pressure. J. Cereb Blood Flow and Metab 12:491-499 |
98. Harder DR, Lombard JH. (1992) Role of ionic mechanisms in activating arterial and arteriolar smooth muscle. In Biology of the Ocular Microcirculation: Excerpta Medica. (RN Weinreb, ed), Elsevier Science Publishers, Amsterdam, pp. 43-48 |
99. Harder DR, Roman RS. (1992) Pressure-induced activation of cerebral and renal arteries. In: Endothelial Regulation of Vascular Tone. (U. Ryan and G. Rubanyi eds.). Marcel Dekker, New York. pp. 265-271 |
100. Harder DR, Schulte ML, Clough AV, Dawson CA. (1992) An angiographic method for in vivo study of arteries of the Circle of Willis in small animals. Am J Physiol 263 (Heart Circ Physiol 32): H1616-H1622 |
101. Harder DR, Gebremedhin D, Ma Y-H, Roman RS. (1992) Signal transduction responses to pressure in arterial muscle: A possible role for arachidonic acid metabolites. In: Ion Channels of Vascular Smooth Muscle Cells and Endothelial Cells (N. Sperelakis and H. Kuriyamaeds) Elsevier, New York, pp. 235-238 |
102. Gebremedhin D, Ma Y-H, Falck JR, Roman RJ, VanRollins M, Harder DR. (1992) Mechanism of action of cerebral epoxyeicosatrienoic acids on cerebral arterial smooth muscle. Am J Physiol 263(Heart Circ Physiol 32): H519-H525 |
103. Ma Y-H, Gebremedhin D, Schwartzman ML, Falck JR, Clark JE, Masters BS, Harder DR, Roman RJ. (1993) 20-Hydroxyeicosatetraenoic acid is an endogenous vasoconstrictor of canine renal arcuate arteries. Circ Res 72:126-136 |
104. Imig JD, Falck JR, Gebremedhin D, Harder DR, Roman RJ. (1993) Elevated renovascular tone in young spontaneously hypertensive rats: Role of cytochrome P450. Hypertension 22:357-364 |
105. Imig JD, Gebremedhin D, Harder DR, Roman RJ. (1993) Modulation of vascular tone in renal microcirculation by erythrocytes: Role of EDRF. Am J Physiol 264 (Heart Circ Physiol 33): H190-H195 |
106. Harder DR (1993) Electrophysiological mechanisms of cerebral arterial muscle activation: Influences of environmental stimuli. In: The Regulation of Cerebral Blood Flow Control. (J W Philis, ed). Boca Raton, Florida: CRC Press, pp 33-44 |
107. Roman RJ, Harder DR. (1993) Cytochrome P450 metabolites of arachidonic acid in the control of renal vascular tone. In: Mechanoreception by the Vascular Wall. (GM Rubanyi, ed). Mount Kisco, NY: Futura Pub Inc, pp. 155-172 |
108. Gebremedhin D, Ma Y-H, Imig JD, Harder DR, Roman JR. (1993) Role of cytochrome P450 in elevating renal vascular tone in spontaneously hypertensive rats. J Vasc Res 30:53-60 |
109. Masters BSS, Clark JE, Roman LJ, Nishimoto M, McCabe TJ, Ortiz de Montellano PR, Plopper CG, Gebremedhin D, Ma Y-H, Harder DR, Roman RJ. (1993) Functional aspects of eicosanoid hydroxylation by lung and kidney cytochrome P450. Expression of cDNAs in mammalian cells and E. coli. J Lipid Mediators 6:353-360 |
110. Roman RJ, Harder DR. (1993) Cellular and ionic signal transduction pathways for mechanical activation of renal arterial vascular smooth muscle. J Am Soc Nephrol 4:986-996 |
111. Harder DR, Gebremedhin D, Roman RJ. (1993) Influence of physical and environmental factors on ion channels in arterial muscle. In: Ion Flux in Pulmonary Vascular Control. (EK Weir, ed) Plenum Publishing Corporation. pp. 105-110 |
112. Narayanan J, Imig M, Roman RJ, Harder DR. (1994) Pressurization of isolated renal arteries increases inositol triphosphate and diacylglycerol. Am J Physiol 266 (Heart Circ Physiol 35): H1840-H1845 |
113. Harder DR, Gebremedhin D, Narayanan J, Jefcoat C, Falck JR, Roman RJ. (1994) Formation and action of a P450 4A metabolite of arachidonic acid in cat cerebral microvessels. Am J Physiol 266 (Heart Circ Physiol 35): H2098-H2107 |
114. Eskinder H, Fredricks KT, Kauser K, Harder DR, Lombard JH. (1994) Electrical and mechanical responses to endothelin in small arteries of the dog kidney. J Endothel Cell Res 2:67,72. |
115. Harder DR. (1994) Neuroprotective effects of tetrodotoxin as a Na+ channel modulator and glutamate release inhibitor in cultured rat cerebellar neurons and in gerbil global brain ischemia - Comment. Stroke 25: (12) 2482. |
116. Li P, Zou A-P, Al-Kayed NJ, Rusch J, Harder DR. (1994) Guanine nucleotide-binding proteins in aortic smooth muscle from hypertensive rats. Hypertension 23(2): 914-918 |
117. Liu Y, Harder DR, Lombard JH. (1994) Myogenic activation of canine small renal arteries after nonchemical removal of the endothelium. Am H Physiol: Heart & Circ 267 (Heart Circ Physiol 36): H302-H307 |
118. Gebremedhin D, Bonnet P, England SK, Rusch N, Lombard JH, Harder DR. (1994) Hypoxia increases the activity of Ca2+ - sensitive K+ channel in cat cerebral arterial muscle cells membranes. Pflugers Arch 428:621-630 |
119. Harder DR, Campbell WB, Roman RJ. (1995) Role of cytochrome P450 enzymes and metabolites of arachidonic acid in the control of vascular tone. J Vasc Res 32:79-92 |
120. Jacobs ER, Cheliakine C, Gebremedhin D, Davies PF, Harder DR. (1995) Shear activated channels in cell-attached patches of cultured bovine aortic endothelial cells. Pflugers Arch 431:129-131. |
121. Pratt PF, Gebremedhin D, Harder DR, et al. (1995) Epoxyeicosatrienoic acids are endothelium-derived hyperpolarizing factors. Hypertension 26: (3) 549. |
122. Harder DR, Narayanan J, Gebremedhin D, Roman RJ. (1995) Transduction of physical force by the vascular wall: Role of phospholipase C and cytochrome P450 metabolites of arachidonic acid. Trends in Cardiovascular Medicine 5(1): 7-14 |
123. Campbell WB, Gebremedhin D, Pratt PF, Harder DR. (1996) Identification of epoxyeicosatrienoic acids as endothelium-derived hyperpolarizing factors. Circ Res. 78:415-423. |
124. Harder DR. (1996) Relaxation of subarachnoid hemorrhage-induced spasm of rabbit basilar artery by the K+ channel activator cromakalim - Editorial comment. Stroke 27: (2) 316. |
125. Imig JD, Zou A-P, Stec DE, Harder DR, Falck JR, Roman RJ. (1996) Formation and actions of 20-hydroxyeicosatetraenoic acid in rat renal arterioles. Am J Physiol (Reg Int Physiol) 270:R217-R227 |
126. Gebremedhin D, Kaldunski M, Jacobs ER, Harder DR, Roman RJ. (1996) Coexistence of two types of Ca2+ - activated K+ channels in rat renal arterioles. Am J Physiol (Renal, Fluid, Electrol Physiol) 270:F69-F81 |
127. Pearce WJ, Harder DR. (1996) Cerebrovascular smooth muscle and endothelium. In: The Neurophysiological Basis of Cerebral Blood Flow Control: An Introduction. (R Sercombe, S Mraovitch, eds) John Libbey, LTD, London. |
128. Harder DR, Narayanan J, Birks EK, Liard JF, Imig JD, Lombard JH, Lange AR, Roman RJ. (1996) Identification of a putative microvascular oxygen sensor. Circ Res. 79:54-61 |
129. Alkayed NJ, Narayanan J, Gebremedhin D, Medhora M, Roman RJ, Harder DR. (1996) Molecular characterization of an arachidonic acid epoxygenase in rat brain astrocytes. Stroke 27:971-979. |
130. Zou AP, Fleming JT, Falck JR, Jacobs ER, Gebremedhin D, Harder DR, Roman RJ. (1996) 20-HETE is an endogenous inhibitor of the large conductance Ca2+-activated K+ channel in renal arterioles. Am J Physiol (Regulatory Integrative Comp. Physiol.) 270:R228-R237 |
131. Alkayed NJ, Birks EK, Hudetz AG, Roman RJ, Henderson L, Harder DR. (1996) Inhibition of brain P450 arachidonic acid epoxygenase decreases baseline cerebral blood flow. Amer J Physiol. (Heart Circ. Physiol.) 271:H1541-H1546 |
132. Zou AP, Fleming JT, Falck JR, Jacobs ER, Gebremedhin D, Harder DR, Roman RJ. (1996). Stereospecific effects of epoxyeicosatrienoic acids on renal vascular tone and K+ channel activity. Am J Physiol. 270 (Renal Fluid and Electrolyte Physiol 39): F822-F832. |
133. Harder DH, Campbell WB, Gebremedhin D, Pratt PF. (1997) Bioassay of an endothelial cytochrome P450-dependent hyperpolarizing factor from bovine coronary arteries. In: Endothelium-Derived Hyperpolarizing Factor. (P.M. Vanhoutte, editor) Harwood Academics Publishers, The Netherlands, pp: 73-80. |
134. Alkayed NJ, Birks EK, Narayanan J, Petrie KA, Kohler-Cabot AE, Harder DR. (1997) Role of P450 arachidonic acid epoxygenase in the response of cerebral blood flow to glutamate. Stroke. 1997; 28(5): 1066-1072 |
135. Harder DH, Lange AR, Gebremedhin D, Birks EK, Roman RJ. (1997) Cytochrome P450 metabolites of arachidonic acid as intracellular signaling molecules in vascular tissue. J. Vasc. Res. 34(3): 237-243. |
136. Lange AR, Gebremedhin D, Narayanan J, Harder DR. (1997) 20-hydroxyeicosatetraenoic acid (20-Hete) induced vasoconstriction and inhibition of potassium (K+) current in cerebral vascular smooth muscle is dependent on activation of protein kinase C (PKC). J Biol. Chem. 272(43): 27345-27352. |
137. Harder DR. (1997) Brain injury impairs ATP-sensitive K+ channel function in piglet cerebral arteries - Editorial comment. Stroke 28: (11) 2280. |
138. Campbell WB, Pratt PE, Gebremedhin D, Harder DR. (1996) Epoxyeicosatrienoic acids are endothelium-derived hyperpolarizing and vasodilating factors in bovine coronary arteries. Endothelium-Derived Hyperpolarizing Factor. (P.M. Vanhoutte, editor) Harwood Academic Publishers, The Netherlands, pp: 81-89. |
139. Heistad DD and Harder DR. (1997) Introduction - Symposium on potassium channels in blood vessels. Clin. Exp. Pharmacol. Physiol. In Press |
140. Birks EK, Zhu D, Parviz M, Bousamra M, Patel M, Presberg K, Effros RM, Harder DR, Roman RJ, Jacobs ER. (1997) Hypoxic pulmonary vasoconstriction in rabbit lungs is modulated by endogenous 20-HETE. Am J Physiol. Submitted. |
141. Gebremedhin D, Lange AR, Narayanan J, Aebly MR, Jacobs ER, Harder DR. (1998) Cat cerebral arterial smooth muscle cells express cytochrome P450 4A2 enzyme and produce the vasoconstrictor 20-HETE which enhances L-type Ca2+ current. Journal of Physiology 507(3): 771-781. |
142. Gebremedhin D, Campbell WB, Pratt PF, Jacobs ER, Harder DR. (1998) Bioassay of an endothelium derived hyperpolarizing factor from bovine coronary arteries: Role of a cytochrome P450 metabolite. J Vasc Res 35(4): 274-284. |
143. Zhu D, Effros R, Harder DR, Roman R, Jacobs ER. (1998) Tissue sources of cytochrome P4504A and 20-HETE synthesis in rabbit lungs. Am. J. Resp. Cell & Mol Biology. 19(1): 121-128. |
144. Harder DR, Alkayed NJ, Lange AR, Gebremedhin D, Roman RJ. (1998) Functional Hyperemia in the Brain: Hypothesis for Astrocyte-Derived Vasodilator Metabolites. Stroke 29(1): 229-234. |
145. Sun CW, Alonso-Galicia M, Taheri MR, Falck JR, Harder DR, Roman RJ. (1998) Nitric Oxide-20-HETE Interaction in the Regulation of K+ Channel Activity and Vascular Tone in Renal Arterioles. Circ. Res., 1998; 83(11): 1069-1079. |
146. Harder DR, Roman RJ, Gebremedhin D, Birks EK, Lange AR. (1998) A Common Pathway for Regulation of Nutritive Blood Flow to the Brain: Arterial Muscle Membrane Potential and Cytochrome P450 Metabolites. Acta Physiol Scand. 164(4): 527-532. |
147. Campbell WB and Harder DR. (1999) Endothelium-Derived Hyperpolarizing Factors and Vascular Cytochrome P450 Metabolites of Arachidonic Acid in the Regulation of Tone. Circ Res. 84(4): 484-488. |
148. Harder DR, Roman RJ, Koehler RC, Alkayed N, Gebremedhin D, Munzenmaier D, Medhora M, Narayanan J. (1998) Hyperpolarizing Factors and Membrane Potential in Cerebral Arterioles: A Role for Astrocytes and Cytochrome P450 Genes. In EDHF, Paul M. Vanhoutte (ed), Taylor & Francis: London and New York. |
149. Alonso-Galicia M, Hudetz AG, Shen H, Harder DR, Roman RJ. (1998) A Fall in 20-HETE levels contributes to the vasodilator actions of NO in the cerebral microcirculation. Hypertension 32 (3) 599. |
150. Alonso-Galicia M, Sun CW, Harder DR, et al. (1998) Blockade of 20-HETE formation mimics the vasodilator response to nitric oxide in renal arterioles. Hypertension 32: (3) 616. |
151. Gebremedhin D, Lange AR, Harder DR (1998) 20-hydroxyeicosatetraenoic acid is produced by cerebral arterial muscle cells and translocates PKC alpha and delta to inhibit macroscopic KCa current. J Vasc Res 35: 5-5 Supp. 1. |
152. Medhora M, Harder DR. (1998) Functional role of epoxyeicosatrienoic acids and their production in astrocytes: approaches for gene transfer and therapy (review). Int J Mol Med 2(6) 661-9. |
153. Sun C-W, Falck, JR, Harder DR, Roman RJ. (1999) Role of tyrosine kinase and PKC in the vasoconstrictor response to 20-HETE in Renal Arterioles. Hypertension. 33(1 Pt 2): 414-418. |
154. Fisslthaler B, Popp R, Kiss L, Potente M, Harder DR, Fleming I, Busse R. (1999) Cytochrome p450 2C (CYP2C) is an EDHF synthase in coronary arteries. Nature 401(6752) 493-7. |
155. Alonso-Galicia M, Hudetz AG, Shen H, Harder DR, Roman RJ. (1999) Contribution of 20-HETE to vasodilator actions of nitric oxide in the cerebral microcirculation. Stroke 30(12): 2727-34. |
156. Gebremedhin D, Lange AR, Campbell WB, Hillard CJ, Harder DR. (1999) Cannabinoid CB1 receptor of cat cerebral arterial muscle functions to inhibit L-type Ca2+ channel current. Am J Physiol 276(6 Pt 2): H2085-93. |
157. Smeda JS, King S, Harder DR. (1999) Cerebrovascular alterations in protein kinase C-mediated constriction in stroke-prone rats. Stroke 30(3): 656-61. |
158. Campbell WB, Harder DR. (1999) Endothelium-derived hyperpolarizing factors and vascular cytochrome P450 metabolites of arachidonic acid in the regulation of tone. Circ Res 84(4):484-8. |
159. Lombard JH, Kunert MP, Roman RJ, Falck JR, Harder DR, Jackson WF. (1999) Cytochrome P-450 omega-hydroxylase senses 02 in hamster muscle, but not cheek pouch epithelium, microcirculation. Am J Physiol 276(2 Pt 2): H503-8. |
160. Harder DR. (1999) News from the National Heart, Lung and Blood Institute - Editor's reply. Am J Physiol-Heart C 46: (3) H856. |
161. Harder DR. (1999) A format for translational research. Am J Physiol 276(1 PT 2): H1-2. |
162. Harder, DR, Roman RJ, Koehler RC, Alkayed N, Gebremedhin D, Munzenmaier D, Medhora M, and Narayanan J. (1999) Hyperpolarizing factors and membrane potential in cerebral arterioles: A role for astrocytes and cytochrome P450 genes. Hyperpolarizing Factors. Edited by P.M.Vanhoutte Plenumn Press, NY, pp 61-67. |
163. Sun CW, Falck JR, Okamoto H, Harder DR, Roman RJ. (2000) Role of cGMP versus 20-HETE in the vasodilator response to nitric oxide in rat cerebral arteries. Am J Physiol Heart Circ Physiol 279(1): H339-50. |
164. Zhu D, Bousamra M, Zeldin DC, Falck JR, Townsley M, Harder DR, Roman RJ, Jacobs ER. (2000) Epoxyeicosatrienoic acids constrict isolated pressurized rabbit pulmonary arteries. Am J Physiol Lung Cell Mol Physiol 278(2): L335-43. |
165. Munzenmaier, DH, Harder DR. (2000) Cerebral microvascular endothelial cell tube formation: role of astrocytic epoxyeicosatrienoic acid release. Am J Physiol Heart Circ Physiol 278:H1163-H1167. |
166. Harder DR, Roman RJ, Gebremedhin D. (2000) Molecular mechanisms controlling nutritive blood flow:role of cytochrome P450 enzymes. Acta Physiol Scand 168:543-549. |
167. Harder DR (2000) Impact factors and the competitive nature of journal publishing. Am J Physiol-Heart C 279: (2) H457-H457. |
168. Bhardwaj A. Northington FJ. Carhuapoma JR Falck JR. Harder DR. Traystman RJ. Koehler RC. (2000) P-450 epoxygenase and NO synthase inhibitors reduce cerebral blood flow response to N-methyl-D-aspartate. American Journal of Physiology - Heart & Circulatory Physiology. 279 (4): H1616-24. |
169. Roman RJ. Maier KG. Sun CW. Harder DR. Alonso-Galicia M. (2000) Renal and Cardiovascular actions of 20-hydroxyeicosatrsenoic acid and epoxyeicosatrienoic acids. Clinical & Experimental Pharmacology & Physiology. 27(11): 855-65. |
170. Bhardwaj A. Northington FJ. Carhuapoma JR Falck JR. Harder DR. Traystman RJ. Koehler RC. (2000) P-450 epoxygenase and NO synthase inhibitors reduce cerebral blood flow response to N-methyl-D-aspartate. American Journal of Physiology - Heart & Circulatory Physiology. 279 (4): H1616-24. |
171. Gebremedhin D, Lange AR, Lowry TF, Taheri MR, Birks EK, Hudetz AG, Narayanan J, Falck JR, Okamoto H, Roman RJ, Nithipatikom K, Campbell WB, Harder DR. (2000). Production of 20-HETE and Its Role in Autoregulation of Cerebral Blood Flow. Circ Res. 7; 87(1): 60-5. |
172. Sun CW, Falck JR, Harder DR, and Roman RJ. (2000) Contribution of cGMP versus 20-HETE in mediating K+ channel and vasodilator responses to NO in rat middle cerebral arteries. Am J Physiol 279:H339 50. |
173. Medhora M. Narayanan J. Harder DR. (2001) Dual Regulation of the cerebral microvasculature by epoxyeicosatrienoic acids. Trends in Cardiovascular Medicine 11(1): 38-42. |
174. Medhora M. Narayanan J. Harder DR. Maier KG. (2001) Identifying endothelium-derived hyperpolarizing factor: recent approaches to assay the role of epoxyeicosatrienoic acids. Japan J.Pharmacol 86(4): 369-75. |
175. Harder DR, Zhang C, Taheri MR, Narayanan J, Roman RJ, Voskuil CE, Medhora MM. (2001) Expression of recombinant cytochrome P450 epoxygenase in rat brain. In: EDHF 2000, ed. Paul M. Vanhoutte. (Taylor & Francis: London and New York), Chapter 25, pp.222-227. |
176. Nithipatikom K. Grall AJ. Holmes BB, Harder DR. Falck JR. Campbell WB. (2001) Liquid Chromatographic-electrospray ionization-mass spectrometric analysis of cytochrome p450 metabolites of arachodonic Acid. Anal Biochem 15; 298(2): 327-36. |
177. Harder DR. Zhang C. Gebremedhin D. (2002) Astrocytes function in matching blood flow to metabolic activity. News Physiol Sci 17:27-31 |
178. Zhang C. Harder DH. (2001) Epoxyeicosatrienoic acids released by astrocytes: Function in cerebral angiogenesis Excerpta Medica international Congress series (ICS). (in press) |
179. Zhang C. Harder DR. (2001) Cerebral capillary endothelial cell mitogenesis and morphogenesis induced by astrocytic epoxyeicosatrienoic acid. Stroke 33(12): 2957-64. |
180. Ming Yu, Cheng-Wen Sun, Kristopher G. Maier, Harder DR, Richard Roman. (2001) mechanism of cGMP Contribution to the Vasodilator Response to NO in Rat Middle Cerebral Arteries. AJP Heart and Circulation 282(5): H1724-31. |
181. Campbell WB and Harder DR. (2001) Prologue: EDHF--What is it? Am. J. Physiol. 280(6): H2413-6 |
182. Harder DR, Zhang C and Gebremedhin D. (2002) Astrocytes Function in Matching Blood Flow to Metabolic Activity. News Physiol.Sci. 17:27-31. |
183. Kehl F, Cambj-Sapunar L, Maier KG, Miyata N, Kametani S. Okamoto H, Hudetz AG, Schulte ML, Zagorac D, Harder DR, Roman RJ. (2002) 20-HETE contributes to the acute fall in cerebral blood flow after subarachnoid hemorrhage in the rat. Am. J. Physiol. 282(4): H1556-65. |
184. Xinqi Peng, Juan R. Carhuapoma, Anish Bhardwaj, Nabil J. Alkayed, John R. Falck, Harder DR, Richard J. Traystman, and Raymond C. Koehler. (2002) Suppression of Cortical Functional Hyperemia to Vibrissal Stimulation in the Rat by Epoxygenase Inhibitors. Am. J. Physiol 283(5): H2029-37. |
185. Johan Bylund, Chenyang Zhang and Harder Dr. (2002) Identification of a novel Cytochrome P450, CYP4X1, with Unique Localization Specific to the Brain. Biochem Biophy Res Commun. 296(3) 677-684. |
186. Zhang C, Harder Dr. (2002) Cerebral Capillary Endothelial Cell Mitogenesis and Morphogenesis Induced by Astrocytic Epoxyeicosatrienoic Acid. Stroke 33(12): 2957-64 |
187. Liu, Y, Harder DR, Lombard JH (2002). Interaction of Myogenic Mechanisms and Hypoxic Dilation in Rat Middle Cerebral Arteries. Am J. Physiol Heart Circ. Physiol. 283(6): H2276-81 |
188. Xinqi Peng, Juan R. Carhuapoma, Anish Bhardwaj, Nabil J. Alkayed, John R. Falck, Harder DR, Richard J. Traystman, and Raymond C. Koehler. (2002) Regulation pf the cerebral circulation by cytochrome P450 epoxygenase activity. In Brain Activation and Cerebral Blood Flow Control. M. Tomita (ed), Amsterdm, Elsevier Science B.V. (Symposium). |
189. Medhora MM, Daniels J, Mundey K, Fisslthaler B, Busse R, Jacobs ER, Harder DR. (2003) Epoxygenase Driven Angiogenesis in Human Lung Microvascular Endothelial Cells. Am J. Physiol Heart Circ. 284(1): H215-24. |
190. Alkayed NJ, Goyagi T, Joh HD, Klaus J, Harder DR, Traystman RJ, Hurn PD. (2002) Neuroprotection and P450 2C11 upregulation after experimental transient ischemic attack. Stroke Jun; 33(6):1677-84 |
191. Michaelis UR. Fisslthaler B. Medhora M. Harder DR. Fleming I. Busse R. (2003) Cytochrome P450 2C9-derived epoxyeicosatrienoic acids induce angiogenesis via cross-talk with the epidermal growth factor receptor. FASEB J 2003. |
192. Bylund J, Harder AG, Maier KG, Roman RJ, Harder Dr. (2003) Leukotriene B(4) omega-side chain hydroxylation by CYP4F5 and CYP4F6. Arch Biochem Biophys Apr 1; 412(1): 34-41. |
193. Gebremedhin D, Yamaura K, Zhang C, Bylund J, Koehler RC, Harder DR. (2003) Metabotropic glutamate receptor activation enhances the activities of two types of Ca2+-activated K+ channels in rat hippocampal astrocytes. J Neurosc :23(5): 1678-87. |
194. Campur-Sapar L, Yu M, Maier KM, Harder DR, Roman RJ. (2003) Contribution of 5HT1b receptors and 20-HETE to the fall in cerebral blood flow following SAH. Stroke 34(5):1269-75. |
195. Campur-Sapar L, Yu M, Kehl F, Harder DR, Roman RJ. (2004) Effects of 20-HETE agonists and antagonists on cerebral blood flow. Eur J. Pharmacol 486(3):297-306. |
196. Peng X, Zhang C, Alkayed NJ, Harder DR, Koehler RC. (2004) Dependency of Cortical Functional Hyperemia to Forepaw Stimulation of Epoxygenase and Nitric Oxide Synthase Activities in Rats. Journal of Cerebral Blood Flow & Metabolism 24(5):509-517. |
197. Drummond A, Gebremedhin D, Harder Dr. (2004) Degenerin/Epithelial Na+ Channel Proteins: Components of a Vascular Mechanosensor. Hypertension 2004;44:643-648) |
198. Pratt PF, Medhora M, Harder Dr. (2004) Mechanisms regulating cerebral blood flow as therapeutic targets. Current Opinion in Investigational Drugs 5(9):952-956 |
199. Miyata N, Seki T, Tanaka Y, Omura T, Taniguchi K, Doi M, Bandou K, Kametani S, Sato M, Okuyama S, Cambj-Sapunar L, Harder DR, Roman RJ. (2005) Beneficial effects of a new 20-hydroxyeicosatetraenoic acid synthesis inhibitor, TS-011 [N-(3-chloro-4-morpholin-4-yl) phenyl-N'-hydroxyimido formamide], on hemorrhagic and ischemic stroke. J Pharmacol Exp Ther. Jul;314(1):77-85. |
200. Takeuchi K, Miyata N, Renic M, Harder DR, Roman RJ. (2005) Hemoglobin, NO and 20-HETE Interactions in Mediating Cerebral Vasoconstriction following SAH. Am J Physiol Regul Integr Comp Physiol 2901):R84-9. |
201. Takeuchi K, Renic M, Bohman QC, Harder DR, Miyata N, Roman RJ. (2005) Reversal of delayed vasospasm by an inhibitor of the synthesis of 20-HETE. Am J Physiol Heart Circ Physiol 289(5):H2203-11. |
202. Zagorac D, Yamaura K, Zhang C, Roman RJ, Harder Dr. (2005) The Effect of Superoxide Anion on Autoregulation of Cerebral Blood Flow. Stroke 36:2589-2594 |
203. Koehler RC, Gebremedhin D, Harder DR. (2006) Role of astrocytes in cerebrovascular regulation. J Appl Physiol 100(1):307-17. |
204. Omura T, Tanaka Y, Miyata N, Koizumi C, Sakurai T, Fukasawa M, Hachiuma K, Minagawa T, Susumu T, Yoshida S, Nakaike S, Okuyama S, Harder DR, Roman RJ. (2006) Effect of a new inhibitor of the synthesis of 20-HETE on cerebral ischemia reperfusion injury. Stroke 37(5):1307-13. |
205. Harder DR, Roman RJ. (2006) Endothelial dysfunction and hypertension in rats transduced with CYP4A2 adenovirus. Circ Res 14;98(7):866-7. |
206. Iadecola C, Goldman SS, Harder DR, Heistad DD, Katusic ZS, Moskowitz MA, Simard JM, Sloan MA, Traystman RJ, Velletri PA. (2006) Recommendations of the National Heart, Lung, and Blood Institute working group on cerebrovascular biology and disease. Stroke 37(6):1578-81. |
207. Hacien-Bey L, Harder DR, Meier HT, Varelas PN, Miyata N, Lauer KK, Cusick JF and Roman RJ. (2006) Reversal of delayed vasospasm by TS-011 in the dual hemorrhage dog model of subarachnoid hemorrhage. AJNR Am J Neuroradiol 27(6):1350-4. |
208. Yamaura K, Gebremedhin D, Zhang C, Narayanan J, Hoefert K, Jacobs ER, Koehler RC, Harder DR. (2006) Contribution of epoxyeicosatrienoic acids to the hypoxia-induced activation of Ca2+ -activated K+ channel current in cultured rat hippocampal astrocytes. Neuroscience 143(3):703-16. |
209. Terashvili M, Pratt PF, Gebremedhin D, Narayanan J, Harder DR. (2006) Reactive oxygen species cerebral autoregulation in health and disease. Pediatr Clin North Am 53(5):1029-37, xi. Review. |
210. Medhora M, Dhanasekaran A, Gruenloh SK, Dunn LK, Gabrilovich M, Falck JR, Harder DR, Jacobs ER, Pratt PF. (2007) Emerging mechanisms for growth and protection of the vasculature by cytochrome P450-derived products of arachidonic acid and other eicosanoids. Prostaglandins Other Lipid Mediat 82(1-4):19-29. |
211. Thomas S, Kotamraju S, Zielonka J, Harder DR, Kalyanaraman B. (2007) Hydrogen peroxide induces nitric oxide and proteosome activity in endothelial cells: a bell-shaped signaling response. Free Radic Biol Med 42(7):1049-61. |
212. Stowe DF, Camara AK, Heisner JS, Aldakkak M, Harder DR. (2007) Ten-hour preservation of guinea pig isolated hearts perfused at low flow with air-saturated Lifor® solution at 26oC: comparison to ViaSpan®. Am J Physiol Heart Circ Physiol 293(1): H895-901. |
213. Jakovcevic D, Harder DR. (2007) Role of astrocytes in matching blood flow to neuronal activity. Curr Top Dev Biol 79:75-97. |
214. Shi Y, Liu X, Gebremedhin D, Falck JR, Harder DR, Koehler RC. (2008) Interaction of mechanisms involving epoxyeicosatrienoic acids, adenosine receptors, and metabotropic glutamate receptors in neurovascular coupling in rat whisker barrel cortex. J Cereb Blood Flow Metab 28(1): 111-25. |
215. Terashvili, Maia, Wu, Hsiang-en, Moore, Rachel M., Harder, David R (2007) Tseng, Leon F., (2007) (+)-Morphine and (-)-morphine stereoselectively attenuate the (-)-morphine-produced tail-flick inhibition via the naloxone-sensitive sigma receptor in the ventral periaqueductal gray of the rat. European Journal of Pharmacology 571(1):1-7.. |
216. Gebremedhin D, Yamaura K, Harder DR. (2008) Role of 20-HETE in the hypoxia-induced activation of Ca2+-activated K+ channel currents in rat cerebral arterial muscle cells. Am J Physiol Heart Circ Physiol 294(1):H107-20. |
217. Zagorac D, Jakovcevic D, Gebremedhin D, Harder DR (2008) Antiangiogenic effects of inihibitors of cytochrome P450 on rats with glioblastoma multiforme. J Cereb Blood Flow Metab 28(8):1431-9. |
218. Terashvili M, Tseng LF, Wu HE, Narayanan J, Hart LM, Flack JR, Pratt PF, Harder, DR (2008) Antinociception produced by 14,15-epoxyeicosatrienoic acid is mediated by the activation of beta-endorphin and met-enkephalin in the rat ventrolateral periqueductal gray. J Pharmacol Exp Ther 326 (2):614-22. |
219. Liu X, Li C, Falck JR, Roman RJ, Harder DR, Koehler RC (2008). Interaction of nitric oxide, 20-HETE, and EETs during functional hyperemia in whister barrel cortex. Am J Physiol Heart Circ Physiol 295(2): H619-31. |
220. Prasda VV, Nithipatikom K, Harder DR (2008) Cereamide elevates 12-hydroxyeicosatetraenoic acid levels and upregulates 12-lipoxygenase in rat primary hippocampal cell cultures containing predominantly astrocytes. Neurochem Int53(6-8): 220-9. |
221. Toyama K, Wulff H, Chandy KG, Azam P, Raman G, Saito R, Fujiwara Y, Mattson DL, Das S, Melvin JE, Pratt PF, Hatoum OA, Gutterman DD, Harder DR, Miura, H (2008). The intermediate-conductance calcium-activated potassium channel KCa3.1 contributes to atherogenesis in mice and humans. J Clin Invest 118(9):3025-37. |
222. Athirakul K, Bradbury Dagger JA, Graves JP, Degraff LM, Ma J, Zhao Y, Couse JF, Quigley R, Harder DR, Zhao X, Imig JD, Pederson TL, Newman JW, Hammock BD, Conley AJ, Korach KS, Coffman TM, Zeldin DC (2008). Increased blood pressure in mice lacking cytochrome P450 2J5. FASEB J 22 (12)4096-108. |
223. Stowe DF,Camara AK, Heisner JS, Aldakkak M, Harder DR (2008) Low-flow perfusion of guinea pig isolated hearts with 26˚C air-saturated Lifor® solution for 20 hours preserves function and metabolism. J Heat and Lung Transplantation 27 (9):1008-15. PubMed Central PMCID: PMC2720038. |
224. Dunn KM, Renic M, Flasch AK, Harder DR, Falck J (2008) Elevated production of 20-HETE in the cerebral vasculature contributes to severity of ischemic stroke and oxidative stress in spontaneously hypertensive rats. Am J Physiol Heart Circ Physiol 295: H2455-H2465. PubMed Central PMCID: PMC2614536. |
225. Renic M, Klaus JA, Omura T, Kawashima N, Onishi M, Miyata N, Koehler RC, Harder DR, and Roman RJ (2009). Effect of 20-HETE inhibition on infarctvolume and cerebral blood flow after transient middle cerebral artery occlusion. J Cereb Blood Flow Metab. 29(3): 629-39. PubMed Central PMCID: PMC2821901. |
226. Koehler RD, Roman RJ, Harder DR (2009) Astrocytes and the regulation of cerebral blood flow. Trends in Neurosciences 32 (3): 169-9. |
227. Cao S, Wang LC, Kwansa H, Roman RJ, Harder DR, Koehler RC (2009). Endothelin rather than 20-HETE contributes to loss of pial arteriolar dilation during focal cerebral ischemia with and without polymeric hemoglobin transfusion. Am J Physiol Regul Integr Comp Physiol 296(5):R1412-18. PubMed Central PMCID: PMC2689832. |
228. Gebremedhin D, Weinberger B, Lourim D, Harder DR (2010) Adenosine can mediate its actions through generation of reactive oxygen species. J Cereb Blood Flow Metab. 2010 Jun 9. PubMed Central PMCID: PMC3023402. |
229. Ohata H, Gebremedhin D, Narayanan J, Harder DR, Koehler RC (2010) Onset of pulmonary ventilation in fetal sheep produces pial arteriolar constriction dependent on cytochrome P450 {omega}-hydroxylase activity. J Appl Physiol. 2010 May 20. PubMed Central PMCID: PMC2928585. |
230. Gebremedhin D, Narayanan J, Harder DR, (2011) Pressure-Induces Myogenic Tone and Role of 20-HETE in Mediating Autoregulation of Cerebral Blood Flow. Am J Physiol Heart Circ Physiol. 2011 Jan 21 [PubMed, PMID: 21257913] |
231. Imig, JD, Simpkins AN, Renic M, Harder DR, (2011) Cytochrome P450 eicosanoids and cerebral vascular function. Expert Rev Mol Med 2011 Mar 1 [PubMed, PMID: 21356152] |
232. Liu X, Li C, Gebremedhin D, Hwang SH, Hammock BD, Falck JR, Roman RJ, Harder DR, Koehler RC (2011) Epoxyeicosatrienoic acid-dependent cerebral vasodilation evoked by metabotropic glutamate receptor activation in vivo. Am J Physiol Heart Circ Physiol. 2011 [PubMed, PMID: 21602473]. |
233. Sarkar P, Narayanan J, Harder DR (2011) Differential effect of amyloid beta on the cytochrome P450 epoxygenase activity in rat brain. Neuroscience. 2011 Oct 27 [PubMed, PMID: 21843605]. |
234. Liu X, Li C, Falck JR, Harder DR, Koehler RC (2011) Relative contribution of cyclooxygenases, epoxyeicosatrienoic acids, and pH to the cerebral blood flow response to vibrissal stimulation. Am J Physiol Heart Circ Physiol. 2012 [PMCID: PMC3311453]. |
235. Renic M, Kumar SN, Gebremedhin D, Florence MA, Gerges NZ, Falck JR, Harder DR, Roman RJ. (2012) Protective effect of 20-HETE inhibition in a model of oxygen-glucose deprivation in hippocampal slice cultures. Am J Physiol Heart Circ Physiol. 2012 [PMCID: PMC3311475]. |
236. Yang ZJ, Carter EL, Kibler KK, Kwansa H, Crafa DA, Martin LJ, Roman RJ, Harder DR, Koehler RC. (2012) Attenuation of neonatal ischemic brain damage using a 20-HETE synthesis inhibitor. J Neurochem. 2012 [PMCID: PMC3303996]. |
237. Terashvili M, Sarkar P, Nostrand MV, Falck JR, Harder DR. (2012) The protective effect of astrocyte-derived 14,15-EET on H(2)O(2)-induced cell injury in Astrocyte-dopaminergic neuronal cell line co-culture. Neuroscience. 2012 Jul 31 [PubMed, PMID:22863680]. |
238. Harder DR, Narayanan J, Gebremedhin D (2011). Pressure-induced myogenic tone and role of 20-HETE in mediating autoregulation of cerebral blood flow. Am J Physiol Heart Circ Physiol. 2011 [PMCID: PMC3283039]. |
239. Zhang Y, Hong G, Lee KS, Hammock B, Gebremedhin D, Koehler R, Harder DR, Sapirstein A Inhibition of soluble epoxide hydrolase augments astrocyte release of vascular endothelial growth factor and neuronal recovery after oxygen –glucose deprivation Journal of Neurochemistry. |
240. Gebremedhin D, David Zhang X, Carver K, Rau N, Roman, R, Harder DR Expression of CYP 4A ω- hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE) in cultured rat brain astrocytes Elsevier . |
241. Gebremedhin D, Wickramasekera NT, and Harder DR Reactive oxygen species modulate astrocyte and capillary cell functions in the regulation of cerebral blood flow. In: Hand Book of Systems Biology of Oxidative Stress Hand Book of Systems Biology of Oxidative Stress. |
242. Heistad DD, Harder DR Symposium on potassium channels in blood vessels Clinical Experimental Pharmacology Physiology . |
243. Zhang C. Harder DH Epoxyeicosatrienoic acids released by astrocytes: Function in cerebral angiogenesis Excerpta Medica International Congress Series (ICS). |
244. Harder DR, Narayanan J, Gebremedhin D, Roman RJ. Transduction of physical force by the vascular wall Role of phospholipase C and cytochrome P450 metabolites of arachidonic acid. Trends Cardiovasc Med. 1995;5(1):7-14. |
245. Campbell WB, Gebremedhin D, Pratt PF, Harder DR. Identification of epoxyeicosatrienoic acids as endothelium-derived hyperpolarizing factors. Circ Res. 1996 Mar;78(3):415-23. |
246. Jacobs ER, Cheliakine C, Gebremedhin D, Birks EK, Davies PF, Harder DR. Shear activated channels in cell-attached patches of cultured bovine aortic endothelial cells. Pflugers Arch. 1995 Nov;431(1):129-31. |
247. Harder DR, Campbell WB, Roman RJ. Role of cytochrome P-450 enzymes and metabolites of arachidonic acid in the control of vascular tone. J Vasc Res. 1995;32(2):79-92. |
248. Gebremedhin D, Bonnet P, Greene AS, England SK, Rusch NJ, Lombard JH, Harder DR. Hypoxia increases the activity of Ca(2+)-sensitive K+ channels in cat cerebral arterial muscle cell membranes. Pflugers Arch. 1994 Oct;428(5-6):621-30. |
249. Liu Y, Harder DR, Lombard JH. Myogenic activation of canine small renal arteries after nonchemical removal of the endothelium. Am J Physiol. 1994 Jul;267(1 Pt 2):H302-7. |
250. Harder DR, Gebremedhin D, Roman RJ nfluence of physical and environmental factors on ion channels in arterial muscle Ion Flux in Pulmonary Vascular Control. |
251. Harder DR, Roman RJ, Lombard JH Cellular and biochemical mechanisms of pressure-induced myogenic tone in arteries Resistance Arteries, Structure and Function: Excerpta Medica. |
252. Masters BSS, Clark JE, Roman LJ, McCabe TJ, Helm CB, Johnson EF, Ma Y-H, Harder DR, Roman RJ Structure-function studies and physiological roles of eicosanoids metabolized by cytochrome P450 w-Hydroxylases Prostaglandins, Leukotrienes, Lipoxins and PAF. |
253. Gebremedhin D, Zhang DX, Weihrauch D, Uche NN, Harder DR. Detection of TRPV4 channel current-like activity in Fawn Hooded hypertensive (FHH) rat cerebral arterial muscle cells. PLoS One. 2017;12(5):e0176796. PMCID: PMC5417564 |
254. Zhang H, Falck JR, Roman RJ, Harder DR, Koehler RC, Yang ZJ. Upregulation of 20-HETE Synthetic Cytochrome P450 Isoforms by Oxygen-Glucose Deprivation in Cortical Neurons. Cell Mol Neurobiol. 2017 Oct;37(7):1279-1286. PMCID: PMC5522363 |
255. Zhang Y, Hong G, Lee KS, Hammock BD, Gebremedhin D, Harder DR, Koehler RC, Sapirstein A. Inhibition of soluble epoxide hydrolase augments astrocyte release of vascular endothelial growth factor and neuronal recovery after oxygen-glucose deprivation. J Neurochem. 2017 Mar;140(5):814-825. PMCID: PMC5310989 |
256. Gebremedhin D, Zhang DX, Carver KA, Rau N, Rarick KR, Roman RJ, Harder DR. Expression of CYP 4A ω-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE) in cultured rat brain astrocytes. Prostaglandins Other Lipid Mediat. 2016 Jul;124:16-26. PMCID: PMC5287377 |
Editorials, Letters To Editor, Other |
1. Harder DR (2004). Ca-induced Ca release: lessons regarding cell models. American Physiological Society. 10.1152/classicessays.00008.2004 |
