Medical College of Wisconsin
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CURRICULUM VITAE

Gopinath Tata PhD
Assistant Professor
Department of Biophysics

OFFICE ADDRESS:
MACC Fund Research Center
8701 Watertown Plank Rd
Milwaukee, WI 53226

EDUCATION:
06/1999 - 06/2001 MSc, University of Hyderabad, India
06/2002 - 08/2007 PhD, Indian Institute of Science, Bangalore, India

POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS:
11/01/2007 - 05/30/2010 Postdoctoral Fellow, Biochemistry, Molecular Biology and Biophysics (BMBB), University of Minnesota, MN
06/01/2010 - 05/30/2012 Research Associate, Biochemistry, Molecular Biology and Biophysics (BMBB), University of Minnesota, MN

FACULTY APPOINTMENTS:
06/2012 - 05/2021 Research Assistant Professor, Minnesota NMR Center, BMBB, Medical School, University of Minnesota, MN
09/01/2022 - Present Assistant Professor, Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI

EDUCATIONAL ADMINISTRATIVE APPOINTMENTS:
06/2012 - 05/2021 Instructor for NMR courses, Minnesota NMR Center, University of Minnesota, MN

RESEARCH ADMINISTRATIVE APPOINTMENTS:
06/2012 - 05/2021 NMR Manager, Minnesota NMR Center, University of Minnesota, MN
06/2021 - 05/2022 Senior Scientist, Biochemistry Department, University of Wisconsin Madison, WI

AWARDS AND HONORS:
11/2010 Cyrus P. Barnum and Anne R. Barnum Postdoctoral Travel Award, BMBB Department, University of Minnesota
11/2011 Paul D. Boyer - James B. Peter Award, BMBB Department University of Minnesota
07/2012 Technical innovation award, College of Biological Sciences, University of Minnesota

INVITED LECTURES/WORKSHOPS/PRESENTATIONS:
International
Fast multi-dimensional solid-state NMR experiments for membrane protein structure Determination, NMR meets biology: Discussion meeting on NMR and challenging biology issues, Goa, India, 02/21/2014 - 02/27/2014
Probing membrane protein ground and conformationally excited states using dipolar and J-coupling mediated MAS solid state NMR experiments, NMR meets biology: Discussion meeting on NMR and challenging biology issues, Khajuraho, Madhya Pradesh, India, 12/16/2018 - 12/18/2018
 
National
Methodological developments in solid state NMR of biomacromolecules, Solid State NMR Symposium at Rocky Mountain Conference on Analytical Chemistry, Copper Conference Center in Copper Mountain, Colorado, 07/15/2012 - 07/19/2012
Dual acquisition magic angle spinning solid-state NMR: A new approach to high-throughput structure determination of proteins, Symposium on "Atomic View of Bimolecular Function", University of Michigan, Ann Arbor, 07/11/2013 - 07/14/2013
 
Regional
Multiple Acquisitions of Magic Angle Spinning Solid-State NMR Experiments Using One Receiver: Application to Microcrystalline and Membrane Proteins, Chicago Area NMR Discussion Group, Northwestern University, Evanston, Illinois, 2014
 
Local
Dual acquisition MAS solid-state NMR experiments, The 14th Bollum Symposium, University of Minnesota, 05/23/2012
Solid-State NMR methods: Application to polymer science, Food Science and Nutrition Department, University of Minnesota, 12/20/2013
 

PEER REVIEWED WORKSHOPS/PRESENTATIONS:
International
Gopinath T, Das R, Kumar A, Programmable quantum state discriminator using nuclear magnetic resonance, International conference on magnetic resonance in Biological systems (ICMRBS), Hyderabad, India, 02/2005
 
National
Gopinath T, Das R, Kumar A, Programmable Quantum information processing by NMR: Implementation of Inversion-on-equality gate, Parity gate and Fanout gate, National magnetic resonance society (NMRS) conference, Kolkatta, India, 02/2004
Kumar A, Gopinath T, Programmable Hadamard Encoding for Parallel Search, Preparation of Pseudo-Pure States and Two-Dimensional Quantum Gates and Algorithms, Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, California, 04/2006
Gopinath T, Kumar A, Hadamard NMR spectroscopy for two-dimensional quantum information processing and parallel search algorithms, National magnetic resonance society (NMRS) conference, Pune, India, 02/2007
Gopinath T, Kumar A, Liouville Space Search Algorithm: Experimental Implementation in Strongly Coupled spins, Experimental Nuclear Magnetic Resonance Conference, Daytona Beach, Florida, 04/2007
Gopinath T, Traaseth NJ, Verardi R, Mote KR, Veglia G, Sensitivity enhancement in solid state NMR of aligned membrane proteins, Experimental Nuclear Magnetic Resonance Conference, Daytona Beach, Florida, 04/2010
Gopinath T, Veglia G, Proton evolved local field solid state NMR using Hadamard encoding, Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, California, 03/2011
Dual Acquisition Magic Angle Spinning (DUMAS) Solid-State NMR: A new approach to simultaneous acquisition of multi-dimensional spectra of Biomacromolecules, Experimental Nuclear Magnetic Resonance Conference, Miami, Florida, 04/15/2012 - 04/20/2012
Gopinath T, Veglia G, Orphan Spin Operators Enable the Acquisition of Multiple 2D and 3D MAS Solid-State NMR Spectra, Experimental Nuclear Magnetic Resonance Conference, Pacific Grove, California, 03/2013
Multiple Acquisitions of Magic Angle Spinning Solid-State NMR Experiments Using One Receiver: Application to Microcrystalline and Membrane Proteins, Experimental Nuclear Magnetic Resonance Conference, Pittsburgh, Pennsylvania, 04/10/2016 - 04/15/2016
Gopinath T, Nelson SE, Soller KJ, Veglia G, Conformationally Excited States of Membrane Proteins via 1H-detected MAS Solid-State NMR Spectroscopy, Experimental Nuclear Magnetic Resonance Conference, Asilomar Conference Center, Pacific Grove, CA, 03/26/2017 - 03/31/2017
 


BIBLIOGRAPHY
Refereed Journal Publications/Original Papers
1. Gopinath T, Das R, Kumar A. Quantum information processing by NMR: Implementation of Inversion-on-equality gate, Parity gate and Fanout gate Proc. Indian Natn. Sci. Acad. 2004. 70:649-658.
2. Gopinath T, Das R, Kumar A. Programmable quantum-state discriminator by nuclear magnetic resonance Physical Review A - Atomic, Molecular, and Optical Physics. April 2005;71(4).
3. Gopinath T, Kumar A. Geometric quantum computation using fictitious spin- 1 2 subspaces of strongly dipolar coupled nuclear spins Physical Review A - Atomic, Molecular, and Optical Physics. 2006;73(2).
4. Gopinath T, Kumar A. Hadamard NMR spectroscopy for two-dimensional quantum information processing and parallel search algorithms. J Magn Reson. 2006 Dec;183(2):259-68.
5. Gopinath T, Kumar A. Implementation of Liouville Space Search Algorithm on a strongly dipolar Coupled Nuclear Spins "Future Directions of Magnetic Resonance" Special issue to commemorate the Platinum Jubilee of the Indian National Science Academy, Springer. 2009.
6. Gopinath T, Kumar A. Implementation of controlled phase shift gates and Collins version of Deutsch-Jozsa algorithm on a quadrupolar spin-7/2 nucleus using non-adiabatic geometric phases. J Magn Reson. 2008 Aug;193(2):168-76.
7. Gopinath T, Veglia G. Sensitivity enhancement in static solid-state NMR experiments via single- and multiple-quantum dipolar coherences. J Am Chem Soc. 2009 Apr 29;131(16):5754-6. PMCID: PMC3481552
8. Gopinath T, Traaseth NJ, Mote K, Veglia G. Sensitivity enhanced heteronuclear correlation spectroscopy in multidimensional solid-state NMR of oriented systems via chemical shift coherences. J Am Chem Soc. 2010 Apr 21;132(15):5357-63. PMCID: PMC3328406
9. Gopinath T, Verardi R, Traaseth NJ, Veglia G. Sensitivity enhancement of separated local field experiments: Application to membrane proteins Journal of Physical Chemistry B. 22 April 2010;114(15):5089-5095.
10. Gopinath T, Veglia G. Improved resolution in dipolar NMR spectra using constant time evolution PISEMA experiment Chemical Physics Letters. 9 July 2010;494(1-3):104-110.
11. Traaseth NJ, Gopinath T, Veglia G. On the performance of spin diffusion NMR techniques in oriented solids: Prospects for resonance assignments and distance measurements from separated local field experiments Journal of Physical Chemistry B. 4 November 2010;114(43):13872-13880.
12. Gopinath T, Mote KR, Veglia G. Proton evolved local field solid-state nuclear magnetic resonance using Hadamard encoding: theory and application to membrane proteins. J Chem Phys. 2011 Aug 21;135(7):074503. PMCID: PMC3172034
13. Mote KR, Gopinath T, Traaseth NJ, Kitchen J, Gor'kov PL, Brey WW, Veglia G. Multidimensional oriented solid-state NMR experiments enable the sequential assignment of uniformly 15N labeled integral membrane proteins in magnetically aligned lipid bilayers. J Biomol NMR. 2011 Nov;51(3):339-46.
14. Gopinath T, Veglia G. Dual acquisition magic-angle spinning solid-state NMR-spectroscopy: simultaneous acquisition of multidimensional spectra of biomacromolecules. Angew Chem Int Ed Engl. 2012 Mar 12;51(11):2731-5. PMCID: PMC3513283
15. Gopinath T, Veglia G. 3D DUMAS: simultaneous acquisition of three-dimensional magic angle spinning solid-state NMR experiments of proteins. J Magn Reson. 2012 Jul;220:79-84. PMCID: PMC3487463
16. Veglia G, Traaseth NJ, Shi L, Verardi R, Gopinath T, Gustavsson M. The hybrid solution/solid-state NMR method for membrane protein structure determination Comprehensive Biophysics. 2012;1:182-198.
17. Gopinath T, Mote KR, Veglia G. Sensitivity and resolution enhancement of oriented solid-state NMR: application to membrane proteins. Prog Nucl Magn Reson Spectrosc. 2013 Nov;75:50-68. PMCID: PMC3850070
18. Gopinath T, Veglia G. Orphan spin operators enable the acquisition of multiple 2D and 3D magic angle spinning solid-state NMR spectra. J Chem Phys. 2013 May 14;138(18):184201. PMCID: PMC3663853
19. Mote KR, Gopinath T, Veglia G. Determination of structural topology of a membrane protein in lipid bilayers using polarization optimized experiments (POE) for static and MAS solid state NMR spectroscopy. J Biomol NMR. 2013 Oct;57(2):91-102. PMCID: PMC3883141
20. Gustavsson M, Verardi R, Mullen DG, Mote KR, Traaseth NJ, Gopinath T, Veglia G. Allosteric regulation of SERCA by phosphorylation-mediated conformational shift of phospholamban. Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17338-43. PMCID: PMC3808617
21. Fusco G, De Simone A, Gopinath T, Vostrikov V, Vendruscolo M, Dobson CM, Veglia G. Direct observation of the three regions in α-synuclein that determine its membrane-bound behaviour. Nat Commun. 2014 May 29;5:3827. PMCID: PMC4046108
22. Cero C, Vostrikov VV, Verardi R, Severini C, Gopinath T, Braun PD, Sassano MF, Gurney A, Roth BL, Vulchanova L, Possenti R, Veglia G, Bartolomucci A. The TLQP-21 peptide activates the G-protein-coupled receptor C3aR1 via a folding-upon-binding mechanism. Structure. 2014 Dec 02;22(12):1744-1753. PMCID: PMC4353613
23. Gopinath T, Veglia G. Multiple acquisition of magic angle spinning solid-state NMR experiments using one receiver: application to microcrystalline and membrane protein preparations. J Magn Reson. 2015 Apr;253:143-53. PMCID: PMC4399235
24. Vostrikov VV, Soller KJ, Ha KN, Gopinath T, Veglia G. Effects of naturally occurring arginine 14 deletion on phospholamban conformational dynamics and membrane interactions. Biochim Biophys Acta. 2015 Jan;1848(1 Pt B):315-22. PMCID: PMC4258429
25. Gopinath T, Mote KR, Veglia G. Simultaneous acquisition of 2D and 3D solid-state NMR experiments for sequential assignment of oriented membrane protein samples. J Biomol NMR. 2015 May;62(1):53-61. PMCID: PMC4981477
26. Mistry P, Mohapatra S, Gopinath T, Vogt FG, Suryanarayanan R. Role of the Strength of Drug-Polymer Interactions on the Molecular Mobility and Crystallization Inhibition in Ketoconazole Solid Dispersions. Mol Pharm. 2015 Sep 08;12(9):3339-50.
27. Gopinath T, Veglia G. Orphan Spin Polarization: A Catalyst for High-Throughput Solid-State NMR Spectroscopy of Proteins Annual Reports on NMR Spectroscopy. 2016;89:103-121.
28. Vostrikov VV, Gustavsson M, Gopinath T, Mullen D, Dicke AA, Truong V, Veglia G. Ca(2+) ATPase Conformational Transitions in Lipid Bilayers Mapped by Site-directed Ethylation and Solid-State NMR. ACS Chem Biol. 2016 Feb 19;11(2):329-34. PMCID: PMC4993155
29. Veglia G, Nelson SE, Gopinath T. Allosteric Regulation of the M2 Protein from Influenza A by Cholesterol. Biophys J. 2016 Mar 29;110(6):1207-8. PMCID: PMC4816697
30. Gopinath T, Veglia G. Multiple acquisitions via sequential transfer of orphan spin polarization (MAeSTOSO): How far can we push residual spin polarization in solid-state NMR? J Magn Reson. 2016 Jun;267:1-8. PMCID: PMC4862926
31. Dicke A, Gopinath T, Wang Y, Veglia G. Probing Residue-Specific Water-Protein Interactions in Oriented Lipid Membranes via Solid-State NMR Spectroscopy Journal of Physical Chemistry B. 27 October 2016;120(42):10959-10968.
32. Gopinath T, Nelson SED, Soller KJ, Veglia G. Probing the Conformationally Excited States of Membrane Proteins via <sup>1</sup>H-Detected MAS Solid-State NMR Spectroscopy. J Phys Chem B. 2017 May 04;121(17):4456-4465.
33. Gopinath T, Nelson SED, Veglia G. <sup>1</sup>H-detected MAS solid-state NMR experiments enable the simultaneous mapping of rigid and dynamic domains of membrane proteins. J Magn Reson. 2017 Dec;285:101-107. PMCID: PMC5764182
34. Gopinath T, Veglia G. Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins. Methods Mol Biol. 2018;1688:37-53.
35. Nelson SED, Ha KN, Gopinath T, Exline MH, Mascioni A, Thomas DD, Veglia G. Effects of the Arg9Cys and Arg25Cys mutations on phospholamban's conformational equilibrium in membrane bilayers. Biochim Biophys Acta Biomembr. 2018 Jun;1860(6):1335-1341. PMCID: PMC6428084
36. Wang S, Gopinath T, Veglia G. Application of paramagnetic relaxation enhancements to accelerate the acquisition of 2D and 3D solid-state NMR spectra of oriented membrane proteins. Methods. 2018 Apr 01;138-139:54-61. PMCID: PMC5984106
37. Gopinath T, Veglia G. Probing membrane protein ground and conformationally excited states using dipolar- and J-coupling mediated MAS solid state NMR experiments. Methods. 2018 Sep 15;148:115-122. PMCID: PMC6428079
38. Weber DK, Bader T, Larsen EK, Wang S, Gopinath T, Distefano M, Veglia G. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy. Methods Enzymol. 2019;621:281-304. PMCID: PMC7418180
39. Duggirala NK , Li J , Kumar NSK , Gopinath T , Suryanarayanan R . A supramolecular synthon approach to design amorphous solid dispersions with exceptional physical stability. Chem Commun (Camb). 2019 May 09;55(39):5551-5554.
40. Anderson EL, Lodge TP, Gopinath T, Veglia G, Bühlmann P. More than a Liquid Junction: Effect of Stirring, Flow Rate, and Inward and Outward Electrolyte Diffusion on Reference Electrodes with Salt Bridges Contained in Nanoporous Glass. Anal Chem. 2019 Jun 18;91(12):7698-7704.
41. Gopinath T, Wang S, Lee J, Aihara H, Veglia G. Hybridization of TEDOR and NCX MAS solid-state NMR experiments for simultaneous acquisition of heteronuclear correlation spectra and distance measurements. J Biomol NMR. 2019 Apr;73(3-4):141-153. PMCID: PMC6526076
42. Hall JW, Lima BP, Herbomel GG, Gopinath T, McDonald L, Shyne MT, Lee JK, Kreth J, Ross KF, Veglia G, Herzberg MC. An intramembrane sensory circuit monitors sortase A-mediated processing of streptococcal adhesins. Sci Signal. 2019 May 07;12(580). PMCID: PMC6662179
43. Wang S, Gopinath T, Veglia G. Improving the quality of oriented membrane protein spectra using heat-compensated separated local field experiments. J Biomol NMR. 2019 Nov;73(10-11):617-624. PMCID: PMC6861693
44. V S M, Gopinath T, Wang S, Veglia G. T<sub>2</sub>* weighted Deconvolution of NMR Spectra: Application to 2D Homonuclear MAS Solid-State NMR of Membrane Proteins. Sci Rep. 2019 Jun 03;9(1):8225. PMCID: PMC6546711
45. Larsen EK, Weber DK, Wang S, Gopinath T, Blackwell DJ, Dalton MP, Robia SL, Gao J, Veglia G. Intrinsically disordered HAX-1 regulates Ca<sup>2+</sup> cycling by interacting with lipid membranes and the phospholamban cytoplasmic region. Biochim Biophys Acta Biomembr. 2020 Jan 01;1862(1):183034. PMCID: PMC6899184
46. Gopinath T, Veglia G. Proton-detected polarization optimized experiments (POE) using ultrafast magic angle spinning solid-state NMR: Multi-acquisition of membrane protein spectra. J Magn Reson. 2020 Jan;310:106664. PMCID: PMC7003683
47. Gopinath T, Weber DK, Veglia G. Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning. J Biomol NMR. 2020 May;74(4-5):267-285. PMCID: PMC7236978
48. Ahmed R, Huang J, Weber DK, Gopinath T, Veglia G, Akimoto M, Khondker A, Rheinstädter MC, Huynh V, Wylie RG, Bozelli JC Jr, Epand RM, Melacini G. Molecular Mechanism for the Suppression of Alpha Synuclein Membrane Toxicity by an Unconventional Extracellular Chaperone. J Am Chem Soc. 2020 May 27;142(21):9686-9699.
49. Gopinath T, Veglia G. Simultaneous acquisition of multiple fast MAS solid-state NMR experiments via orphan spin polarization Annual Reports on NMR Spectroscopy. January 2021;102:247-268.
50. Gopinath T, Weber D, Wang S, Larsen E, Veglia G. Solid-State NMR of Membrane Proteins in Lipid Bilayers: To Spin or Not To Spin? Acc Chem Res. 2021 Mar 16;54(6):1430-1439. PMCID: PMC11457538
51. Weber DK, Reddy UV, Wang S, Larsen EK, Gopinath T, Gustavsson MB, Cornea RL, Thomas DD, De Simone A, Veglia G. Structural basis for allosteric control of the SERCA-Phospholamban membrane complex by Ca<sup>2+</sup> and phosphorylation. Elife. 2021 May 12;10. PMCID: PMC8184213
52. Wang S, Gopinath T, Larsen EK, Weber DK, Walker C, Uddigiri VR, Mote KR, Sahoo SK, Periasamy M, Veglia G. Structural basis for sarcolipin's regulation of muscle thermogenesis by the sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase. Sci Adv. 2021 Nov 26;7(48):eabi7154. PMCID: PMC8626070
53. Reddy UV, Weber DK, Wang S, Larsen EK, Gopinath T, De Simone A, Robia S, Veglia G. A kink in DWORF helical structure controls the activation of the sarcoplasmic reticulum Ca<sup>2+</sup>-ATPase. Structure. 2022 Mar 03;30(3):360-370.e6. PMCID: PMC8897251
54. Gopinath T, Manu VS, Weber DK, Veglia G. PHRONESIS: A One-Shot Approach for Sequential Assignment of Protein Resonances by Ultrafast MAS Solid-State NMR Spectroscopy. Chemphyschem. 2022 Jul 05;23(13):e202200127. PMCID: PMC9400877
55. Weber DK, Larsen EK, Gopinath T, Veglia G. Hybridizing isotropic and anisotropic solid-state NMR restraints for membrane protein structure determination Solid-State NMR: Applications in biomembrane structure. 3 December 2020:12.1-12.21.
56. Gopinath T, Shin K, Tian Y, Im W, Struppe J, Perrone B, Hassan A, Marassi FM. Solid-state NMR MAS CryoProbe enables structural studies of human blood protein vitronectin bound to hydroxyapatite. J Struct Biol. 2024 Mar;216(1):108061. PMCID: PMC10939839
57. Dehinwal R, Gopinath T, Smith RD, Ernst RK, Schifferli DM, Waldor MK, Marassi FM. A pH-sensitive motif in an outer membrane protein activates bacterial membrane vesicle production. Nat Commun. 2024 Aug 13;15(1):6958. PMCID: PMC11322160
58. Gopinath T, Kraft A, Shin K, Wood NA, Marassi FM. Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding. bioRxiv. 2024 Jul 24. PMCID: PMC11360888
 
Books, Chapters, and Reviews
1. Weber DK, Larsen EK, Gopinath T, Veglia G (2020) Hybridizing isotropic and anisotropic solid-state NMR restraints for membrane protein structure determination, Solid-State NMR Applications in biomembrane structure, chapter 12, pages 12-1 to 12-21.
2. Ghosh A, Das R, Gopinath T, Kumar A (2006) Use of Geometric Phase in Quantum information Processing by Nuclear Magnetic Resonance, Proceedings of Workshop on 'Quantum Information, Computation and Communication, Eds. S.P. Pal and S. Kumar, Allied Publishers, India, 57-98.