CURRICULUM VITAE
Jimmy B. Feix PhD
Jimmy B. Feix PhD
Professor
Department of Biophysics
Department of Biophysics
OFFICE ADDRESS: |
Medical Education Building |
8701 Watertown Plank Rd |
Milwaukee, WI 53226 |
EDUCATION: |
08/1972 - 05/1976 BS - Chemistry, Western Kentucky University, Bowling Green, KY |
08/1976 - 05/1981 PhD - Chemistry, University of Kentucky, Lexington, KY |
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS: |
08/1980 - 05/1981 Instructor, Department of Chemistry, University of Kentucky, Lexington, KY |
08/1981 - 07/1983 Muscular Dystrophy Association Postdoctoral Fellow, Department of Radiology, Medical College of Wisconsin, Milwaukee, WI |
08/1983 - 06/1985 Postdoctoral Fellow, National Biomedical ESR Center, Medical College of Wisconsin, Milwaukee, WI |
FACULTY APPOINTMENTS: |
07/1986 - 06/1992 Research Assistant Professor, Department of Radiology, Medical College of Wisconsin, Milwaukee, WI 53226 |
07/1992 - 06/1998 Associate Professor of Biophysics, Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, WI 53226 |
07/1999 - Present Professor of Biophysics, Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226 |
AWARDS AND HONORS: |
1976 Summa Cum Laude, B.S. |
1976 - 1977 R. P. Murrill Research Fellowship, University of Kentucky |
1976 Ogden Scholar (Valedictorian, Ogden College of Science and Technology), Western Kentucky University |
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES: |
1981 - Present International EPR Society (Member) |
1984 - Present American Chemical Society (Member) |
1986 - Present Biophysical Society (Member) |
EDITORSHIPS/EDITORIAL BOARDS/JOURNAL REVIEWS: |
Journal Review |
Biochemistry |
Journal of Biological Chemistry |
Nature Structural Biology |
Proc. Natl. Acad. Sci., USA |
Journal of the American Chemical Society |
Archives of Biochemistry and Biophysics |
Biochimica et Biophysica Acta |
Biophysical Journal |
Free Radicals in Biology and Medicine |
Protein Science |
NATIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS: |
1995 Extramural Review Panel, NIH/NINDS |
1998 BBCB Study Section, NIH/NIGMS |
2000 BBCB Study Section, NIH/NIGMS |
2001 NSF, Division of Molecular and Cellular Biosciences |
2003 Special Emphasis Panel, NIH, Ruth L. Kirchstein National Research Service Awards |
2003 Extramural Review Panel, NIH/NINDS |
2004 BBCB Study Section, NIH/NIGMS |
Reviewer, Special Emphasis Panel ZGM1 MDCN-Q (04) M |
Reviewer, Special Emphasis Panel ZRG1 BCMB-D (40) L |
Reviewer, Special Emphasis Panel ZGM1 RCB-X (SCORE) |
Reviewer, Special Emphasis Panel ZRG1 BCMB-S (40) P |
RESEARCH GRANTS/AWARDS/CONTRACTS/PROJECTS: | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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INVITED LECTURES/WORKSHOPS/PRESENTATIONS: |
International |
Spin-Trapping Lipid Radicals in Membranes, Universite de Quebec a Trois Rivieres, 03/27/1990 |
Site-Directed Spin Labeling of the Bacterial Iron Transport Protein, FepA, International Conference on the Synthesis, Properties, and Applications of Nitroxides, Kaiserslautern, Germany, 09/25/2001 |
Antimicrobial Peptide – Membrane Interactions, 5th International Meeting on EPR Spectroscopy, Krakow, Poland, 10/2004 |
Synthetic Antimicrobial Peptides, Asia-Pacific EPR Conference, Cairnes, Australia, 07/19/2008 |
Methods and Applications of Site-Directed Spin Labeling, Workshop at the Asia-Pacific EPR Conference, Cairnes, Australia, 07/19/2008 |
Membrane Structure and Dynamics and the Development of New Chemotherapeutic Agents, First Adriatic Symposium on Biophysical Approaches in Biomedical Studies, Split, Croatia, 08/2014 |
Site-Directed Spin Labeling Studies of Protein-Membrane Interactions, Seventh International Meeting on EPR Spectroscopy, Krakow, Poland, 10/2016 |
Site-Directed Spin Labeling (SDSL) EPR Studies of Protein-Membrane Interactions: Activation of the Bacterial Phospholipase, ExoU, Second Adriatic Symposium on Biophysical Approaches in Biomedical Studies, Split, Croatia, 09/2017 |
National |
Measurements of Lipid Diffusion using 14N:15N Spin-Label Pairs, Rocky Mountain International EPR Conference, Denver, CO, 08/01/1984 |
Reactive Intermediates in Photodynamic Therapy, 23rd Southeastern Magnetic Resonance Conference, Atlanta, GA, 10/19/1991 |
Site-directed Spin Labeling of the Ferric Enterobactin Receptor, FepA, University of Missouri, Kansas City, MO, 10/25/1994 |
Site-Directed Spin Labeling: A Paradigm for Structure-Function Analysis, Department of Molecular and Cell Biology, Oregon Health Sciences University, Portland, OR, 05/07/1996 |
Site-Directed Spin Labeling Characterization of Transmembrane Beta-Strands, Gordon Conference on Magnetic Resonance in Biology and Medicine, Ventura, CA, 02/15/1998 - 02/20/1998 |
Folding of Autonomous Protein Domains in the Ferric Enterobactin Receptor, Department of Chemistry, Florida State University, Talahassee, FL, 01/19/1999 |
Site-Directed Mutagenesis and Spin Labeling of the Ferric Enterobactin Receptor: Correlation with High-Resolution Structure, Department of Physiology and Molecular Biophysics, Vanderbilt University School of Medicine, Nashville, TN, 01/20/1999 |
A Site-Directed Spin Labeling Approach to Membrane Protein Structure and Dynamics, Telluride Workshop on Biological Ion Channels, Telluride, CO, 07/29/2003 |
Antimicrobial Peptides, Telluride Workshop on Biological Ion Channels, Telluride, CO, 07/16/2007 |
Site-Directed Spin Labeling Studies of Antimicrobial Peptide – Membrane Interactions, Meeting of the International EPR Society, Breckinridge, CO, 07/22/2007 |
Site-Directed Spin Labeling Studies of Cecropin-Melittin Antimicrobial Peptides, Department of Chemistry and Biochemistry, Miami University, Oxford, OH, 11/30/2009 |
Structure and conformational dynamics of the Pseudomonas aeruginosa toxin, ExoU, EPR 2010: A Joint Conference of the 14th In Vivo ESR/EPR Spectroscopy & Imaging and the 11th International EPR Spin Trapping/Spin Labeling, San Juan, Puerto Rico, 05/03/2010 |
Regional |
Spin-Membrane Immunoassay of Anti-Ganglioside Antibodies, Purdue University, Indianapolis, IN, 10/23/1986 |
Structure and Stability of the Ferric Enterobactin Receptor, Department of Chemistry, Loyola University, Chicago, IL, 11/19/1995 |
Site-Directed Spin Labeling of Antimicrobial Peptides, Department of Biochemistry and Molecular Biology, Rosalind Franklin University, North Chicago, IL, 10/15/2005 |
Local |
Electron - Electron Double Resonance with a Loop-Gap Resonator, Workshop on Loop-Gap Resonators, Milwaukee, WI, 05/13/1989 |
Electron Paramagnetic Resonance Studies in Biological Systems, Department of Physics, Marquette University, Milwaukee, WI, 04/08/1996 |
Structure and Dynamics of a Ligand-Gated Transmembrane Receptor, Department of Chemistry, Marquette University, Milwaukee, WI, 10/26/2001 |
Synthetic Antimicrobial Peptides – Mechanisms of Action and Clinical Applications, Department of Infectious Disease, Medical College of Wisconsin, Milwaukee, WI, 02/02/2012 |
Activation and Membrane Interactions of the P. aeruginosa cytotoxin, ExoU, Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, 02/02/2015 |
Site-Directed Spin Labeling EPR studies of the bacterial phospholipase, ExoU, National Biomedical EPR Workshop, Milwaukee, WI, 05/2015 |
Membrane Interactions and Activation of the Bacterial Phospholipase, ExoU, Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, 01/25/2019 |
COMMITTEE SERVICE: |
Medical College of Wisconsin |
1991 - 1996 Member, Advisory Committee, Cancer Center Shared Tissue Culture Facility, Medical College of Wisconsin |
1994 - 2000 Member, Biophysics Graduate Program Curriculum Committee, Medical College of Wisconsin |
1995 - 2001 Member, Shared Research Facilities Oversight Committee, Medical College of Wisconsin |
2002 - 2005 Member, Mentoring Committee, Interdisciplinary Graduate Program, Medical College of Wisconsin |
2002 - 2005 Member, Executive Evaluation Committee, Interdisciplinary Graduate Program, Medical College of Wisconsin |
2006 - 2009 Member, Research Affairs Committee, Medical College of Wisconsin |
2014 - Present Member, Admissions Committee, Interdisciplinary Graduate Program, Medical College of Wisconsin |
MCW TEACHING ACTIVITIES: |
Medical Student Education |
1994 - Present Introduction to Electron Paramagnetic Resonance, Department of Biophysics |
2003 - Present Biophysical Techniques, Department of Biophysics |
2005 - Present Biochemistry of the Cell, Interdisciplinary Graduate Program |
Director, Graduate Seminar Program, Biophysics |
Physical Properties and Macromolecular Structure, Department of Biochemistry |
Lipids, Membranes, and Carbohydrates, Department of Biochemistry |
Director, Electron Spin Resonance Journal Club, Department of Biophysics |
EXTRAMURAL TEACHING: |
Medical Student Education |
University of Kentucky, General Chemistry (University level), Department of Chemistry |
MCW STUDENTS, FACULTY, RESIDENTS AND CLINICAL/RESEARCH FELLOWS MENTORED: |
Graduate Students |
PhD Students Advised |
1989 - 1994 Jun Liu, tructure-Function Relationship of the Escherichia coli Ferric Enterobactin Receptor, FepA: Molecular Genetic and Electron Paramagnetic Resonance Spin Label Studies, Medical College of Wisconsin |
1995 - 1998 Candice S. Klug, Structural and Stability Studies on the Ferric Enterobactin Receptor FepA Using Advanced EPR Spectroscopy Techniques, Medical College of Wisconsin |
2011 Heather Kaminski, CM15 Modification: Further Development of AMPs as Novel Antibiotics, Medical College of Wisconsin |
2014 - Present Samantha (Kohn) Gies, Medical College of Wisconsin |
PhD Committees |
1991 - 1997 Hanane Koteiche, Medical College of Wisconsin |
1991 - 1993 Hassane S. Mchaourab, Medical College of Wisconsin |
1992 - 1996 Michael Bigelow, Medical College of Wisconsin |
1993 - 1998 Hua Yuan, Medical College of Wisconsin |
1997 David Potter, [Department of Biochemistry], Medical College of Wisconsin |
2001 - 2005 Amit Kumar, Medical College of Wisconsin |
2002 - 2006 Adam Buchaklian, Medical College of Wisconsin |
2002 Andrew Vila, [Department of Biochemistry], Medical College of Wisconsin |
2002 - 2008 Kasia Broniowska, Medical College of Wisconsin |
2003 Paul Hubbard, [Department of Biochemistry], Medical College of Wisconsin |
2005 Hiromi Sato, [Department of Microbiology and Molecular Genetics], Medical College of Wisconsin |
2005 - 2011 Kathryn Westphal, Medical College of Wisconsin |
2006 - 2011 Marija Raguz, Medical College of Wisconsin |
2006 Guanjie Sun, [Department of Biochemistry], Medical College of Wisconsin |
2007 Chris Veldkamp, [Department of Biochemistry], Medical College of Wisconsin |
2008 Roger Pechous, [Department of Microbiology and Molecular Genetics], Medical College of Wisconsin |
2008 Derek Francis, Medical College of Wisconsin |
2012 Aaron Kittell, Medical College of Wisconsin |
2013 Casey O'connor, [Department of Biochemistry], Medical College of Wisconsin |
2013 Daniel Bretl, [Department of Microbiology and Molecular Genetics], Medical College of Wisconsin |
2014 Ya Zhuo, Medical College of Wisconsin |
2018 Amber Bakkum, [Department of Biochemistry], Medical College of Wisconsin |
2018 Michael Curtis, [Department of Microbiology and Molecular Genetics], Medical College of Wisconsin |
2019 Maxx Tessmer, [Department of Microbiology and Molecular Genetics], Medical College of Wisconsin |
Nicole Minton, [Department of Microbiology and Molecular Genetics], Medical College of Wisconsin |
Christa Winslow, [Department of Microbiology and Molecular Genetics ], Medical College of Wisconsin |
Hayley Powers, [Department of Medicine], Medical College of Wisconsin |
MS Students Advised |
2008 Gayatri Mitchell, Medical College of Wisconsin |
Postdoctoral Researchers |
2002 - 2005 Dr. Kalpana Bhargava, Medical College of Wisconsin |
2005 - 2008 Dr. Hiromi Sato, Medical College of Wisconsin |
2016 - 2018 Tzvia Springer, Medical College of Wisconsin |
EXTRAMURAL STUDENTS, FACULTY, RESIDENTS, AND CLINICAL/RESEARCH FELLOWS MENTORED: |
Graduate Students |
PhD Students Advised |
2008 Sara Pistolesi, Universitá di Siena |
BIBLIOGRAPHY |
Refereed Journal Publications/Original Papers |
1. Feix JB, Butterfield DA. Selective spin labeling of sialic acid residues of glycoproteins and glycolipids in erythrocyte membranes: a novel method to study cell surface interactions. FEBS Lett. 1980 Jun 30;115(2):185-8. |
2. Feix JB, Green LL, Butterfield DA. Effects of phytohaemagglutinin, wheat-germ agglutinin, and concanavalin-A on the physical state of sialic acid and membrane proteins in human erythrocyte ghosts: a spin label study. Life Sci. 1982 Sep 06;31(10):1001-9. |
3. Lohr KM, Feix JB, Kurth C. Chlorpromazine inhibits neutrophil chemotaxis beyond the chemotactic receptor-ligand interaction. J Infect Dis. 1984 Nov;150(5):643-52. |
4. Ashraf J, Feix JB, Butterfield DA. Membrane fluidity and myotonia: effects of cholesterol and desmosterol on erythrocyte membrane fluidity in rats with 20,25-diazacholesterol-induced myotonia and on phospholipid liposomes. Biosci Rep. 1984 Feb;4(2):115-20. |
5. Butterfield DA, Farmer BT 2nd, Feix JB. Induced alterations in the physical state of sialic acid and membrane proteins in human erythrocyte ghosts: implications for the topology of the major sialoglycoprotein. Ann N Y Acad Sci. 1983;414:169-79. |
6. Feix JB, Khatri B, McQuillen MP, Koethe SM. Immune reactivity against membranes containing ganglioside GM1 in chronic-progressive multiple sclerosis: observation by spin-membrane immunoassay. Immunol Commun. 1984;13(5):465-74. |
7. Feix JB, Popp CA, Venkataramu SD, Beth AH, Park JH, Hyde JS. An electron-electron double-resonance study of interactions between [14N]- and [15N]stearic acid spin-label pairs: lateral diffusion and vertical fluctuations in dimyristoylphosphatidylcholine. Biochemistry. 1984 May 08;23(10):2293-9. |
8. Hyde JS, Yin JJ, Froncisz W, Feix JB. Electron-electron double resonance (ELDOR) with a loop-gap resonator. J Magn Reson. 1 June 1985;63(1):142-150. |
9. Lai CS, Wirt MD, Yin JJ, Froncisz W, Feix JB, Kunicki TJ, Hyde JS. Lateral diffusion of lipid probes in the surface membrane of human platelets. An electron-electron double resonance (ELDOR) study. Biophys J. 1986 Sep;50(3):503-6. PMCID: PMC1329726 |
10. Fajer P, Thomas DD, Feix JB, Hyde JS. Measurement of rotational molecular motion by time-resolved saturation transfer electron paramagnetic resonance. Biophys J. 1986 Dec;50(6):1195-202. PMCID: PMC1329792 |
11. Feix JB, Yin JJ, Hyde JS. Interactions of 14N:15N stearic acid spin-label pairs: effects of host lipid alkyl chain length and unsaturation. Biochemistry. 1987 Jun 30;26(13):3850-5. |
12. Kalyanaraman B, Feix JB, Sieber F, Thomas JP, Girotti AW. Photodynamic action of merocyanine 540 on artificial and natural cell membranes: involvement of singlet molecular oxygen. Proc Natl Acad Sci U S A. 1987 May;84(9):2999-3003. PMCID: PMC304788 |
13. Yin JJ, Feix JB, Hyde JS. The effects of cholesterol on lateral diffusion and vertical fluctuations in lipid bilayers. An electron-electron double resonance (ELDOR) study. Biophys J. 1987 Dec;52(6):1031-8. PMCID: PMC1330102 |
14. Renk GE, Crouch RK, Feix JB. Lack of interaction of rhodopsin chromophore with membrane lipids. An electron-electron double resonance study using 14N:15N pairs. Biophys J. 1988 Mar;53(3):361-5. PMCID: PMC1330204 |
15. Yin JJ, Feix JB, Hyde JS. Solution of the nitroxide spin-label spectral overlap problem using pulse electron spin resonance. Biophys J. 1988 Apr;53(4):525-31. PMCID: PMC1330226 |
16. Feix JB, Kalyanaraman B, Chignell CF, Hall RD. Direct observation of singlet oxygen production by merocyanine 540 associated with phosphatidylcholine liposomes. J Biol Chem. 1988 Nov 25;263(33):17247-50. |
17. Feix JB, Kalyanaraman B. Spin trapping of lipid-derived radicals in liposomes. Biochim Biophys Acta. 1989 Aug 18;992(2):230-5. |
18. Hyde JS, Yin JJ, Feix JB, Hubbell WL. Advances in spin label oximetry. Pure Appl Chem. January 1990;62(2):255-260. |
19. Sheng PG, Feix J, Kalyanaraman B. Characterization of radical adducts formed during photochemical spin trapping in liposomes. Photochem Photobiol. 1990 Aug;52(2):323-31. |
20. Kalyanaraman, B., and Feix, J. B.: The use of Spin Label Oximetry in Photodynamic Studies. Spectrum 3: 9-13, 1990. |
21. Yin JJ, Feix JB, Hyde JS. Mapping of collision frequencies for stearic acid spin labels by saturation-recovery electron paramagnetic resonance. Biophys J. 1990 Sep;58(3):713-20. PMCID: PMC1281012 |
22. Feix JB, Kalyanaraman B. An electron spin resonance study of merocyanine 540-mediated type I reactions in liposomes. Photochem Photobiol. 1991 Jan;53(1):39-45. |
23. Feix JB, Bachowski GJ, Girotti AW. Photodynamic action of merocyanine 540 on erythrocyte membranes: structural perturbation of lipid and protein constituents. Biochim Biophys Acta. 1991 Sep 02;1075(1):28-35. |
24. Singh RJ, Feix JB, Pintar TJ, Girotti AW, Kalyanaraman B. Photodynamic action of merocyanine 540 in artificial bilayers and natural membranes: action spectra and quantum yields. Photochem Photobiol. 1991 Apr;53(4):493-500. |
25. Gaffney DK, Feix JB, Schwarz HP, Struve MF, Sieber F. Cholesterol content but not plasma membrane fluidity influences the susceptibility of L1210 leukemia cells to merocyanine 540-sensitized irradiation. Photochem Photobiol. 1991 Nov;54(5):717-23. |
26. O'Brien JM, Singh RJ, Feix JB, Kalyanaraman B, Sieber F. Action spectra of the antileukemic and antiviral activities of merocyanine 540. Photochem Photobiol. 1991 Nov;54(5):851-4. |
27. Feix JB, Kalyanaraman B. Production of singlet oxygen-derived hydroxyl radical adducts during merocyanine-540-mediated photosensitization: analysis by ESR-spin trapping and HPLC with electrochemical detection. Arch Biochem Biophys. 1991 Nov 15;291(1):43-51. |
28. Singh RJ, Feix JB, Kalyanaraman B. Photobleaching of merocyanine 540: involvement of singlet molecular oxygen. Photochem Photobiol. 1992 Apr;55(4):483-9. |
29. Catallo WJ, Feix JB, Gale RJ. Movement of methylphenazyl free radicals in polar and nonpolar liquids. Free Radic Biol Med. 1992;13(1):35-40. |
30. Rutz JM, Liu J, Lyons JA, Goranson J, Armstrong SK, McIntosh MA, Feix JB, Klebba PE. Formation of a gated channel by a ligand-specific transport protein in the bacterial outer membrane. Science. 1992 Oct 16;258(5081):471-5. |
31. Kalyanaraman B, Ramanujam S, Singh RJ, Joseph J, Feix JB. Formation of 2,5-Dihydroxybenzoic Acid during the Reaction between <sup>1</sup>O<inf>2</inf> and Salicylic Acid: Analysis by ESR Oximetry and HPLC with Electrochemical Detection Journal of the American Chemical Society. 1 May 1993;115(10):4007-4012. |
32. Anderson MS, Kalyanaraman B, Feix JB. Enhancement of merocyanine 540-mediated phototherapy by salicylate. Cancer Res. 1993 Feb 15;53(4):806-9. |
33. Mchaourab HS, Hyde JS, Feix JB. Aggregation state of spin-labeled cecropin AD in solution. Biochemistry. 1993 Nov 09;32(44):11895-902. |
34. Liu J, Rutz JM, Feix JB, Klebba PE. Permeability properties of a large gated channel within the ferric enterobactin receptor, FepA. Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10653-7. PMCID: PMC47835 |
35. Mchaourab HS, Hyde JS, Feix JB. Binding and state of aggregation of spin-labeled cecropin AD in phospholipid bilayers: effects of surface charge and fatty acyl chain length. Biochemistry. 1994 May 31;33(21):6691-9. |
36. Feix JB, Anderson MS, Kalyanaraman B. Enhancement of merocyanine 540 uptake and photodynamic cell killing by salicylates. Cancer Res. 1994 Jul 01;54(13):3474-8. |
37. Liu J, Rutz JM, Klebba PE, Feix JB. A site-directed spin-labeling study of ligand-induced conformational change in the ferric enterobactin receptor, FepA. Biochemistry. 1994 Nov 15;33(45):13274-83. |
38. Singh RJ, Feix JB, Mchaourab HS, Hogg N, Kalyanaraman B. Spin-labeling study of the oxidative damage to low-density lipoprotein. Arch Biochem Biophys. 1995 Jun 20;320(1):155-61. |
39. Klug CS, Su W, Liu J, Klebba PE, Feix JB. Denaturant unfolding of the ferric enterobactin receptor and ligand-induced stabilization studied by site-directed spin labeling. Biochemistry. 1995 Oct 31;34(43):14230-6. |
40. Bose S, Feix J, Seetharam S, Seetharam B. Dimerization of transcobalamin II receptor. Requirement of a structurally ordered lipid bilayer. J Biol Chem. 1996 May 17;271(20):11718-25. |
41. Ferguson, E., Singh, R. J., Hogg, N., Feix, J. B., and Kalyanaraman, B.: Role of Apo-B Thiols during copper and peroxynitrite-mediated oxidation of low-density lipoprotein. Arch. Biochem. Biophys. 323: 477-481, 1997. |
42. Jiang X, Payne MA, Cao Z, Foster SB, Feix JB, Newton SM, Klebba PE. Ligand-specific opening of a gated-porin channel in the outer membrane of living bacteria. Science. 1997 May 23;276(5316):1261-4. |
43. Klug CS, Su W, Feix JB. Mapping of the residues involved in a proposed beta-strand located in the ferric enterobactin receptor FepA using site-directed spin-labeling. Biochemistry. 1997 Oct 21;36(42):13027-33. |
44. Bose S, Chapin SJ, Seetharam S, Feix J, Mostov KE, Seetharam B. Brefeldin A (BFA) inhibits basolateral membrane (BLM) delivery and dimerization of transcobalamin II receptor in human intestinal epithelial Caco-2 cells. BFA effects on BLM cholesterol content. J Biol Chem. 1998 Jun 26;273(26):16163-9. |
45. Klug CS, Feix JB. Guanidine hydrochloride unfolding of a transmembrane beta-strand in FepA using site-directed spin labeling. Protein Sci. 1998 Jun;7(6):1469-76. PMCID: PMC2144043 |
46. Klug CS, Eaton SS, Eaton GR, Feix JB. Ligand-induced conformational change in the ferric enterobactin receptor FepA as studied by site-directed spin labeling and time-domain ESR. Biochemistry. 1998 Jun 23;37(25):9016-23. |
47. Park DS, Petersen CE, Ha C, Harohalli K, Feix JB, Bhagavan NV. Expression of a human serum albumin fragment (consisting of subdomains IA, IB, and IIA) and a study of its properties. IUBMB Life. 1999 Aug;48(2):169-74. |
48. Petersen CE, Ha CE, Harohalli K, Park DS, Feix JB, Isozaki O, Bhagavan NV. Structural investigations of a new familial dysalbuminemic hyperthyroxinemia genotype. Clin Chem. 1999 Aug;45(8 Pt 1):1248-54. |
49. Petersen CE, Ha CE, Harohalli K, Feix JB, Bhagavan NV. A dynamic model for bilirubin binding to human serum albumin. J Biol Chem. 2000 Jul 14;275(28):20985-95. |
50. Zhang H, Joseph J, Feix J, Hogg N, Kalyanaraman B. Nitration and oxidation of a hydrophobic tyrosine probe by peroxynitrite in membranes: comparison with nitration and oxidation of tyrosine by peroxynitrite in aqueous solution. Biochemistry. 2001 Jun 26;40(25):7675-86. |
51. Harohalli K, Petersen CE, Ha CE, Feix JB, Bhagavan NV. Site-directed mutagenesis studies of human serum albumin define tryptophan at amino acid position 214 as the principal site for nitrosation. J Biomed Sci. 2002;9(1):47-58. |
52. Kim YH, Kapfer DM, Boekhorst J, Lubsen NH, Bächinger HP, Shearer TR, David LL, Feix JB, Lampi KJ. Deamidation, but not truncation, decreases the urea stability of a lens structural protein, betaB1-crystallin. Biochemistry. 2002 Nov 26;41(47):14076-84. |
53. Zhang H, Bhargava K, Keszler A, Feix J, Hogg N, Joseph J, Kalyanaraman B. Transmembrane nitration of hydrophobic tyrosyl peptides. Localization, characterization, mechanism of nitration, and biological implications. J Biol Chem. 2003 Mar 14;278(11):8969-78. |
54. Bates IR, Boggs JM, Feix JB, Harauz G. Membrane-anchoring and charge effects in the interaction of myelin basic protein with lipid bilayers studied by site-directed spin labeling. J Biol Chem. 2003 Aug 01;278(31):29041-7. |
55. Sato H, Frank DW, Hillard CJ, Feix JB, Pankhaniya RR, Moriyama K, Finck-Barbançon V, Buchaklian A, Lei M, Long RM, Wiener-Kronish J, Sawa T. The mechanism of action of the Pseudomonas aeruginosa-encoded type III cytotoxin, ExoU. EMBO J. 2003 Jun 16;22(12):2959-69. PMCID: PMC162142 |
56. Bhargava K, Feix JB. Membrane binding, structure, and localization of cecropin-mellitin hybrid peptides: a site-directed spin-labeling study. Biophys J. 2004 Jan;86(1 Pt 1):329-36. PMCID: PMC1303797 |
57. Bates IR, Feix JB, Boggs JM, Harauz G. An immunodominant epitope of myelin basic protein is an amphipathic alpha-helix. J Biol Chem. 2004 Feb 13;279(7):5757-64. |
58. Sato H, Feix JB, Hillard CJ, Frank DW. Characterization of phospholipase activity of the Pseudomonas aeruginosa type III cytotoxin, ExoU. J Bacteriol. 2005 Feb;187(3):1192-5. PMCID: PMC545705 |
59. Sato H, Feix JB. Peptide-membrane interactions and mechanisms of membrane destruction by amphipathic alpha-helical antimicrobial peptides. Biochim Biophys Acta. 2006 Sep;1758(9):1245-56. |
60. Sato H, Feix JB. Osmoprotection of bacterial cells from toxicity caused by antimicrobial hybrid peptide CM15. Biochemistry. 2006 Aug 22;45(33):9997-10007. |
61. Sato H, Feix JB, Frank DW. Identification of superoxide dismutase as a cofactor for the pseudomonas type III toxin, ExoU. Biochemistry. 2006 Aug 29;45(34):10368-75. |
62. Pistolesi S, Pogni R, Feix JB. Membrane insertion and bilayer perturbation by antimicrobial peptide CM15. Biophys J. 2007 Sep 01;93(5):1651-60. PMCID: PMC1948049 |
63. Sato H, Feix JB. Lysine-enriched cecropin-mellitin antimicrobial peptides with enhanced selectivity. Antimicrob Agents Chemother. 2008 Dec;52(12):4463-5. PMCID: PMC2592880 |
64. Subczynski WK, Widomska J, Feix JB. Physical properties of lipid bilayers from EPR spin labeling and their influence on chemical reactions in a membrane environment. Free Radic Biol Med. 2009 Mar 15;46(6):707-18. PMCID: PMC2705953 |
65. Zhang H, Andrekopoulos C, Xu Y, Joseph J, Hogg N, Feix J, Kalyanaraman B. Decreased S-nitrosation of peptide thiols in the membrane interior. Free Radic Biol Med. 2009 Oct 01;47(7):962-8. PMCID: PMC2874255 |
66. Benson MA, Komas SM, Schmalzer KM, Casey MS, Frank DW, Feix JB. Induced conformational changes in the activation of the Pseudomonas aeruginosa type III toxin, ExoU. Biophys J. 2011 Mar 02;100(5):1335-43. PMCID: PMC3043214 |
67. Mainali L, Feix JB, Hyde JS, Subczynski WK. Membrane fluidity profiles as deduced by saturation-recovery EPR measurements of spin-lattice relaxation times of spin labels. J Magn Reson. 2011 Oct;212(2):418-25. PMCID: PMC3214655 |
68. Kaminski HM, Feix JB. Effects of D-Lysine Substitutions on the Activity and Selectivity of Antimicrobial Peptide CM15. Polymers (Basel). 2011;3(4):2088-2106. PMCID: PMC6217857 |
69. Anderson DM, Schmalzer KM, Sato H, Casey M, Terhune SS, Haas AL, Feix JB, Frank DW. Ubiquitin and ubiquitin-modified proteins activate the Pseudomonas aeruginosa T3SS cytotoxin, ExoU. Mol Microbiol. 2011 Dec;82(6):1454-67. PMCID: PMC3237844 |
70. Anderson DM, Feix JB, Monroe AL, Peterson FC, Volkman BF, Haas AL, Frank DW. Identification of the major ubiquitin-binding domain of the Pseudomonas aeruginosa ExoU A2 phospholipase. J Biol Chem. 2013 Sep 13;288(37):26741-52. PMCID: PMC3772220 |
71. Schultz KM, Feix JB, Klug CS. Disruption of LptA oligomerization and affinity of the LptA-LptC interaction. Protein Sci. 2013 Nov;22(11):1639-45. PMCID: PMC3831678 |
72. Anderson DM, Sato H, Dirck AT, Feix JB, Frank DW. Ubiquitin activates patatin-like phospholipases from multiple bacterial species. J Bacteriol. 2015 Feb;197(3):529-41. PMCID: PMC4285982 |
73. Kim SS, Upshur MA, Saotome K, Sahu ID, McCarrick RM, Feix JB, Lorigan GA, Howard KP. Cholesterol-Dependent Conformational Exchange of the C-Terminal Domain of the Influenza A M2 Protein. Biochemistry. 2015 Dec 15;54(49):7157-67. PMCID: PMC4734095 |
74. Anderson DM, Feix JB, Frank DW. Cross Kingdom Activators of Five Classes of Bacterial Effectors. PLoS Pathog. 2015 Jul;11(7):e1004944. PMCID: PMC4512716 |
75. Tessmer MH, Anderson DM, Buchaklian A, Frank DW, Feix JB. Cooperative Substrate-Cofactor Interactions and Membrane Localization of the Bacterial Phospholipase A<sub>2</sub> (PLA<sub>2</sub>) Enzyme, ExoU. J Biol Chem. 2017 Feb 24;292(8):3411-3419. PMCID: PMC5336173 |
76. Fischer AW, Anderson DM, Tessmer MH, Frank DW, Feix JB, Meiler J. Structure and Dynamics of Type III Secretion Effector Protein ExoU As determined by SDSL-EPR Spectroscopy in Conjunction with De Novo Protein Folding. ACS Omega. 2017 Jun 30;2(6):2977-2984. PMCID: PMC5494639 |
77. Herneisen AL, Sahu ID, McCarrick RM, Feix JB, Lorigan GA, Howard KP. A Budding-Defective M2 Mutant Exhibits Reduced Membrane Interaction, Insensitivity to Cholesterol, and Perturbed Interdomain Coupling. Biochemistry. 2017 Nov 07;56(44):5955-5963. PMCID: PMC6112238 |
78. Tessmer MH, Anderson DM, Pickrum AM, Riegert MO, Moretti R, Meiler J, Feix JB, Frank DW. Identification of a ubiquitin-binding interface using Rosetta and DEER. Proc Natl Acad Sci U S A. 2018 Jan 16;115(3):525-530. PMCID: PMC5776994 |
79. Feix JB, Kohn S, Tessmer MH, Anderson DM, Frank DW. Conformational Changes and Membrane Interaction of the Bacterial Phospholipase, ExoU: Characterization by Site-Directed Spin Labeling. Cell Biochem Biophys. 2019 Mar;77(1):79-87. PMCID: PMC6347562 |
80. Xia C, Shen AL, Duangkaew P, Kotewong R, Rongnoparut P, Feix J, Kim JP. Structural and Functional Studies of the Membrane-Binding Domain of NADPH-Cytochrome P450 Oxidoreductase. Biochemistry. 2019 May 14;58(19):2408-2418. PMCID: PMC6873807 |
81. Springer TI, Reid TE, Gies SL, Feix JB. Interactions of the effector ExoU from <i>Pseudomonas aeruginosa</i> with short-chain phosphatidylinositides provide insights into ExoU targeting to host membranes. J Biol Chem. 2019 Dec 13;294(50):19012-19021. PMCID: PMC6916483 |
82. Del Alamo D, Tessmer MH, Stein RA, Feix JB, Mchaourab HS, Meiler J. Rapid Simulation of Unprocessed DEER Decay Data for Protein Fold Prediction. Biophys J. 2020 Jan 21;118(2):366-375. PMCID: PMC6976798 |
83. Tessmer MH, DeCero SA, Del Alamo D, Riegert MO, Meiler J, Frank DW, Feix JB. Characterization of the ExoU activation mechanism using EPR and integrative modeling. Sci Rep. 2020 Nov 12;10(1):19700. PMCID: PMC7665212 |
84. Gies SL, Tessmer MH, Frank DW, Feix JB. Site-Directed Spin Label EPR Studies of the Structure and Membrane Interactions of the Bacterial Phospholipase ExoU Applied Magnetic Resonance. 2023. |
85. Cheng G, Hardy M, Hillard CJ, Feix JB, Kalyanaraman B. Mitigating gut microbial degradation of levodopa and enhancing brain dopamine: Implications in Parkinson's disease. Commun Biol. 2024 May 30;7(1):668. PMCID: PMC11139878 |
86. Gies SL, Tessmer MH, Frank DW, Feix JB. Site-directed spin label EPR studies of the structure and membrane interactions of the bacterial phospholipase ExoU. Appl Magn Reson. 2024 Mar;55(1-3):279-295. PMCID: PMC11340903 |
Books, Chapters, and Reviews |
1. Hyde, J. S., and Feix, J. B., "Electron-Electron Double Resonance", in: Biological Magnetic Resonance, Vol. 8, pp. 305-337, L. J. Berliner and J. Reuben, eds., Plenum Press, 1989. |
2. Feix, J. B. and Klug, C. S., "Site-Directed Spin Labeling of Membrane Proteins and Peptide-Membrane Interactions", in: Spin Labeling: The Next Millennium, L. J. Berliner, Ed., Plenum Press, pp. 251-278, 1998. |
3. Klug, C. S. and Feix, J. B., "SDSL: A Survey of Biological Applications" In: Biological Magnetic Resonance, Volume 24: Biomedical EPR - Part B: Methodology and Instrumentation, S. S. Eaton, G. R. Eaton, L. J. Berliner, eds. Kluwer Academic/Plenum Publishers, pp. 269-308, 2005. |
4. Feix, J.B. and Klug, C.S. "Site-directed spin-labeling applications in biological systems", Royal Society of Chemistry Specialist Periodical Reports, Volume 20: EPR Spectroscopy, B. Gilbert and M. Davies, Eds., Royal Society of Chemistry Press, pp. 51-73 (2007). |
5. Klug CS, Feix JB. Methods and applications of site-directed spin labeling EPR spectroscopy. Methods Cell Biol. 2008;84:617-58. |
Abstracts |
1. Kohn, S., Springer, T. I., and Feix, J. B. “Investigating the conformational dynamics and membrane interaction near the catalytic serine of ExoU upon interaction with diubiquitin and membranes by EPR spectroscopy”. Biophysical J. 114: 614a-615a (2018). 62nd Annual Meeting of the Biophysical Society, San Francisco, CA, February 17 - 21. |
2. Springer, T. I., Kohn, S., and Feix, J. B. “Investigating structural properties of Pseudomonas aeruginaos ExoU toxin upon interaction with liposome and nanodisc bilayers by EPR spectroscopy”. Biophysical J. 114: 614a (2018). 62nd Annual Meeting of the Biophysical Society, San Francisco, CA, February 17 - 21. |
3. Klug, C.S., Feix, J.B., Lerch, M.T., Sidabras, J., Hubbell, W.L., Hyde, J.S. “New high pressure capability at the National Biomedical EPR Center”. 2014 P41 Principal Investigator Meeting, March 24-25, 2014, Rockville, MD. |
4. Kaminski, H. and Feix, J.B. "Biophysical Studies of Cecropin-Mellitin Antimicrobial Peptides with Improved Selectivity." 54th Annual Meeting of the Biophysical Society, San Francisco, CA, February 20 - 24, 2010. |
5. Mitchell, G. and Feix, J.B. "The influence of membrane lipid composition on the mode of action of cecropin-mellitin antimicrobial peptides." 52nd Annual Meeting of the Biophysical Society, Long Beach, CA, March 2-6, 2008. |
6. Pennington PM, Kittell A, Feix J.B., and Hyde, J.S. “Distance determination using L-band EPR”, A Joint Conference of 12th In Vivo EPR Spectroscopy and Imaging and 9th International EPR Spin Trapping/Spin Labeling, Chicago, IL, April 29–May 3, 2007. |
7. Mitchell, G., Pistolesi, S., and Feix, J.B. "Membrane perturbation by the cecropin-mellitin hybrid antimicrobial peptide CM15 and lysine-enriched analogs." 49th Annual Meeting of the International EPR Society, Breckenridge, CO, July 22-26, 2007. |
8. Pennington PM, Kittell A, Feix J.B., and Hyde J.S. “Experimental EPR spectra of nitroxide spin labels at L-band”, 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, CO, July 22–26, 2007. |
9. Sato, H. and Feix, J. B. "Osmoprotection of bacterial cells from toxicity caused by antimicrobial peptide CM15." Biophys. J. 88, 517a (2006). |
10. Kim JJ, Xia C, Hubbard P, Shen AL, Kasper CB, FEIX JB. “Topology and orientation of NADPH cytochrome P450 reductase in the lipid bilayer [abstract]. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, CO, July 22–26, 2007. |
11. Bhargava, K., and Feix, J. B. "Structure and dynamics of cecropin-mellitin hybrid peptides using site-directed spin labeling." Peptide Sci. 80, 535 (2005). |
12. Feix, J. B. and Bhargava, K. "Site-directed spin labeling studies of cecropin-mellitin hybrid peptides", Biophys. J. 86, 484a (2004) |
13. Kumar, A., Feix, J. B., Klug, CS., Holtz, R., and Bennett, B. "Determinants of Substrate Binding in Leucine Aminopeptidase", Gordon Conference on Metal Ions in Biology, Ventura, CA, Jan 18 - 23, 2004. |
14. Bhargava, K., and Feix, J.B. "Membrane binding and antibacterial activity of cecropin-mellitin hybrid peptides." In: Peptides, The Wave of the Future, M. Chorev, Ed. 873, (2004). |
15. Klug, C. S., Su, W., and Feix, J. B., "Ligand-Induced Conformational Changes in the Ferric Enterobactin Receptor, FepA," Biophys. J. 74, A200 (1998). |
16. Klug, C. S., and Feix, J. B., "Saturation Recovery vs. Continuous Wave EPR: A Comparison of Techniques using a -Strand from the Ferric Enterobactin Receptor, FepA," Biophys. J. 74, A300 (1998). |
17. Klug, C. S., Su, W., and Feix, J. B., "Site-directed Spin Labeling studies of β-sheet Structure in the Ferric Enterobactin Receptor, FepA," Protein Sci. 6, 143 (1997). |
18. Klug, C. S., Su, W., and Feix, J. B., "Themodynamic Stability and Tertiary Interaction Studies on FepA using Denaturant Unfolding and Site-Directed Spin Labeling," Biophys. J. 72, A205 (1997). |
19. Klug, C. S., Su, W., and Feix, J. B., "Characterization of a Proposed β-Strand in the Ferric Enterobactin Receptor, FepA by Site-Directed Spin Labeling," Biophys. J. 72, A205 (1997). |
20. Klug, C. S., Su, W., and Feix, J. B., "Guanidine-Induced Denaturation of the Ferric Enterobactin Receptor, FepA," Protein Sci. 4, 168 (1995). |
21. Klebba, P. E., Liu, J., Murphy, C. K., and Feix, J. B., "Structural Dynamics of the Ferric Enterobactin Receptor, FepA, as Studied by Site-Directed Spin Labeling," Biophys. J. 68, A423 (1995). |
22. Liu, J., Rutz, J. M., Klebba, P. E., and Feix, J. B., "Site-Directed Spin Labeling of the Ferric Enterobactin Receptor, FepA," Biophys. J. 65, A69 (1994). |
23. Mchaourab, H. S., Hyde, J. S., and Feix, J. B., "Self-Association and Membrane Binding of Spin Labeled Cecropin AD," Biophys. J. 65, A398 (1994). |
24. Feix, J. B., Anderson, M. S., and Kalyanaraman, B., "Enhancement of Merocyanaine 540 Uptake and Photodynamic Cell Killing by Salicylates," Photochem. Photobiol. 59, 77S (1994). |
25. Liu, J., Rutz, J., Klebba, P., and Feix, J. B., "Reconstitution and Spin Labeling of the E. coli Ferric Enterobactin Receptor", Biophys. J. 64, A167 (1993). |
26. Mchaourab, H. S., Buljubasic, N., Bosnjak, Z., Hyde, J. S., and Feix, J. B., "Interaction of Cecropin AD with Membranes: A Spin Label Study", Biophys. J. 64, A376 (1993). |
27. Kalyanaraman, B., Joseph, J., and Feix, J. B., "Detection of 2,5-Dihydroxybenzioc Acid during the Reaction between Singlet Oxygen and Salicylic Acid," Photochem. Photobiol. 55, 15S (1992). |
28. Feix, J. B. and Kalyanaraman, B., "HPLC and Electron Spin Resonance Spin Trapping Studies of Activated Oxygen Species Produced during Merocyanine 540-Mediated Photosensitization," Photochem. Photobiol. 53, 104S (1991). |
29. Singh, R. J., Feix, J. B. and B. Kalyanaraman, "Photobleaching of Merocyanine 540: Involvement of Singlet Molecular Oxygen," Photochem. Photobiol. 53, 78S (1991). |
30. Bachowski, G. J., Pintar, T. J., Feix, J. B., and Girotti, A. W., "Merocyanine 540-Sensitized Photomodification of Cell Membranes," Photochem. Photobiol. 51, 11S (1990). |
31. Gaffney, D. K., Feix, J. B., Schwarz, H. P., and Sieber, F., "Effects of Lipid Modulation on the Merocyanine 540-Sensitized Photoinactivation of L1210 Leukemia Cells," Photochem. Photobiol. 51, 66S (1990). |
32. Singh, R. J., Feix, J. B., Pintar, T., Girotti, A. W., and Kalyanaraman, B., "Photodynamic Action of Merocyanine 540 in Artificial Bilayers and Natural Membranes: Action Spectra and Quantum Yields," Photochem. Photobiol. 51, 64S (1990). |
33. Feix, J. B. and Kalyanaraman, B., "Merocyanine 540-Mediated Reduction of Nitroxide Spin Labels," Photochem. Photobiol. 49, 81S (1989). |
34. Feix, J. B., Hubbell, C., and Hubbell, W. L., "Motional Dynamics of Stearic Acid Spin Labels and Lipid-Protein Interactions in Bovine Rod Outer Segment Membranes," Biophys. J. 55, 325a (1989). |
35. Feix, J. B., Kalyanaraman, B., and Hall, R. D., "Merocyanine Mediated Photosensitization of Liposomes," Photochem. Photobiol. 45, 79S (1987). |
36. Feix, J. B., Popp, C. A., Hyde, J. S., Venkatramu, S. D., Beth, A., and Park, J. H., "Interactions of 14N and 15N Stearic Acid Spin Labels in Membranes: An EPR ELDOR Experiment," Fed. Proc. 42, 2170 (1983). |
37. Lohr, K. L., Kurth, C., and Feix, J. B., "Effect of Chlorpromazine on the Human Neutrophil Chemotactic Factor Receptor and Membrane Fluidity," Clinical Res. 31, 370 (1983). |