Medical College of Wisconsin
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CURRICULUM VITAE

Linda J. Olson PhD
Assistant Professor
Department of Pharmacology and Toxicology

OFFICE ADDRESS:
Translational and Biomedical Research Center
8701 Watertown Plank Rd
Milwaukee, WI 53226

EDUCATION:
1983 - 1987 BA - Chemistry, Lawrence University, Appleton, WI
1989 - 1991 Doctoral student with Dr. Stephen W. Ragsdale, UW-Milwaukee, Milwaukee, WI
1991 - 1994 Doctoral student with Gregory P. Mullen, UW-Milwaukee, Milwaukee, WI
1994 - Present Ph.D. Chemistry, Dissertation title: Studies of Eukaryotic DNA binding Proteins. Part A: Structure Function Studies on c-Myc Derived Peptides. Part B: Preliminary Studies of the Structure of DNA Polymerase . UW, Milwaukee, WI

POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS:
1987 - 1989 Secondary school teacher of chemistry, physics, general science, St. Lawrence Seminary High School
1988 - 1989 Science Department Chairperson, St. Lawrence Seminary High School
1989 - 1992 Graduate research assistant, The University of Wisconsin-Milwaukee (advisor: Stephen Ragsdale)
1992 - Present Instructor, Mathematics, Med-Prep Program, The University of Wisconsin-Milwaukee
1992 - 1994 Graduate teaching assistant, The University of Wisconsin-Milwaukee (advisor: Dr. Gregory Mullen)
1994 - 1997 Postdoctoral Fellow, Dept. Pharmacology, Medical College of Wisconsin, Milwaukee, WI
1994 - 1997 Postdoctoral fellow, Dept. of Pharmacology, Medical College of Wisconsin (advisor: Dr. Drewett)
1994 - 1995 Lecturer, Survey of Biochemistry, The University of Wisconsin-Milwaukee
1997 - 1999 Postdoctoral Fellow, Dept. Biochemistry, Medical College of Wisconsin, Milwaukee, WI
1997 - 1999 Postdoctoral fellow, Dept. of Biochemistry, Medical College of Wisconsin (advisors: Drs. Dahms and Kim)
1999 - 2000 Senior Research Associate, Dept. of Biochemistry, Medical College of Wisconsin (advisors: Drs. Dahms and Kim)
2000 - 2016 Research Scientist II, Dept. of Biochemistry, Medical College of Wisconsin (advisors: Drs. Dahms and Kim)
2014 - Present X-ray facility manager, Dept. of Biochemistry, Medical College of Wisconsin
2016 - Present Research Track Assistant Professor
Positions and Employment:

AWARDS AND HONORS:
1983 - 1984 Lucia R. Briggs Scholarship, Lawrence University, Appleton, WI
1986 - 1987 Dean's List, Lawrence University, Appleton, WI
2016 - 2017 Graduate Student Award for Outstanding Teacher of the Year, Medical College of Wisconsin
2017 - 2018 Outstanding Graduate School Teacher, Medical College of Wisconsin

MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES:
1989 - 1994 Member (American Association for the Advancement of Science)
1991 - Present American Chemical Society (Member)
1999 - Present Member (American Crystallographic Association)
2019 - Present Member (Society for Glycobiology)
Source: American Heart Association
- 1997 Dates: 1997 - 1999
Title: Natriuretic Peptide Receptors and Aldosterone
Role: Post-Doctoral Fellow in Laboratory of Dr. James G. Drewett
Direct Funds: $41 (000)
Declined due to change of Mentor due to PI relocating
Title: Postdoctoral Fellowship Grant (1 F32 HL09697-01A1)
Source: Department of Health and Human Services
- 1997 Dates: 1997 - 1999
Title: Natriuretic Peptide Receptors and Aldosterone
Role: Post-Doctoral Fellow in Laboratory of Dr. James G. Drewett
Direct Funds: predetermined
Seminar Structure of a Functional Cation-Dependent Mannose 6-Phosphate Receptor Bound to Pentamannosyl Phosphate, American Crystallographic Association, Buffalo NY (1999)
Current
Title: Identification of Novel Glucosidase II Inhibitors to Inhibit Breast Cancer Metastasis
Source: MCW Cancer Center Pilot Award
Dates: July 2017-present
Role: PI
Direct Funds: $50 (000)
Title: Preliminary Studies on the Structure and Function of Human Salt Inducible Kinase 2
Source: Research Affairs Committee (Medical College of Wisconsin)
Dates: August 2018-present
Role: PI
Direct Funds: $ 25 (000)
INVITED PRESENTATIONS:
Local
Seminar, Insights into the Ligand Binding Specificity of the Cation-Dependent Mannose 6-phosphate Receptor. Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (March 1999)
Seminar All in a Day's Work, Structural Insights into the Functioning of CD-MPR, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (May 2001)
Seminar Crystal Structure of the N-terminal Three Domains of the CI-MPR, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (April 2003)
Seminar My Continuing Journey with Mannose 6-Phosphate Receptor and Lysosomal Enzyme Trafficking, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (April 2007)
Seminar Glucosidase II : the Importance of Sugars in the Folding of Proteins in the ER, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (May 2012)
Seminar The Multi-functionality of the IGF2 Receptor: a Biophysical Study of Domains 1-5, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (April 2019.)
National
Title: Postdoctoral Fellowship Grant (97-F-Post-02)
Peer Review
Prior
RESEARCH GRANTS/AWARDS:
Declined due to change of Mentor due to PI relocating


BIBLIOGRAPHY
Refereed Journal Publications/Original Papers
1. Draeger-Olson, L. J. and Mullen G. P. Interaction of the bHLH-Zip Domain of c-Myc with H1-Type Peptides. (1994) J. Biol. Chem. 269,1785-1793. PMID: 8294427
2. Olson L. J., Lowe D. G., and Drewett J. G. Novel natriuretic peptide receptor/guanylyl cyclase A-selective agonist inhibits angiotensin II- and forskolin-evoked aldosterone synthesis in a human zona glomerulosa cell line. (1996) Mol Pharmacol. 50, 430-435. PMID: 8700153
3. Olson, L. J., Knych, Jr., E. T., Herzig, T. C., and Drewtt, J. G. Selective Guanylyl Cyclase Inhibitor Reverses Nitric Oxide-Induced Vasorelaxation. (1997) Hypertension 29, 254-261. PMID: 9039111
4. Olson, L.J., Ho, B.Y, Cashdollar, L.W., and Drewett, J.G. Functionally active catalytic domain is essential for guanylyl cyclase-linked receptor mediated inhibition of human aldosterone synthesis. (1998) Mol Pharmacol. 54, 761-769. PMID: 9804611
5. Olson, L. J., Zhang, J., Lee, Y. C., Dahms, N. M., and Kim, J.-J. P. Structural Basis for Recognition of Phosphorylated High-Mannose Oligosaccharides by the Cation-Dependent Mannose 6-Phosphate Receptor. (1999) J. Biol. Chem. 274, 29889-29896.
6. Olson, L. J., Hancock, M. K., Hubbard, P., Dix, D., Kim, J.-J. P., and Dahms, N. M. Mutational Analysis of the Binding Site Residues of the Bovine Cation-Dependent Mannose 6-Phosphate Receptor. (1999) J. Biol. Chem. 274, 36905-36911.
7. Olson, L. J., Zhang, J., Dahms, N. M., and Kim, J.-J. P. Twists and Turns of the Cation-Dependent Mannose 6-Phosphate Receptor: Ligand-Bound versus Ligand-Free Receptor. (2002) J. Biol. Chem. 277, 10156-10161.
8. Olson, L. J., Yammani, R., Dahms, N. M., and Kim, J.-J. P. (2004) Structure of the uPAR, Plasminogen, and Sugar Binding Sites of the 300 kDa Mannose 6-Phosphate Receptor. EMBO J 23, 2019-2028.
9. Olson, L. J., Dahms, N. M., and Kim, J.-J. P. The N-Terminal Carbohydrate Recognition Site of the Cation-Independent Mannose 6-Phosphate Receptor. (2004) J. Biol. Chem. 279, 34000-34009.
10. Olson, L. J., Hindsgaul, O., Dahms, N. M., and Kim, J.-J. P. Structural Insights into the Mechanism of pH dependent Ligand Binding and Release by the Cation-Dependent Mannose 6-Phosphate Receptor. (2008) J. Biol. Chem. 283, 10124-10134
11. Song, X., Lasanajak, Y., Olson, L. J., Boonen, M., Dahms, N. M., Kornfeld, S., Cummings, R. D., and Smith, D. F. (2009) Glycan Microarray Analysis of P-Type Lectins Reveals Distinct Phospho-Mannose Glycan Recognition J. Biol. Chem. 284, 35201-35214
12. Bohnsack, R. N., Song, X., Olson, L. J., Kudo, M., Gotschall, R. R., Canfield, W. M., Cummings, R. D., Smith, D., F., and Dahms, N. M. Cation-Independent Mannose 6-Phosphate Receptor: A Composite of Distinct Phosphomannosyl Binding Sites J. Biol. Chem. 284, 35215-35226
13. Olson, L. J., Sun, G., Bohnsack, R. N., Peterson, F. C., Dahms, N. M.*, and Kim, J.-J. P.* (2010) Intermonomer Interactions are Essential for Lysosomal Enzyme Binding by the Cation-Dependent Mannose 6-Phosphate Receptor. Biochemistry 49, 236-246. {Manuscript chosen to be highlighted on the journal's home page}
14. Bohnsack, R. N., Patel, M., Olson, L. J., Twinning, S. S., and Dahms, N. M. (2010) Residues Essential for Plasminogen Binding by the Cation-Independent Mannose 6-Phosphate Receptor. Biochemistry 49, 635-644
15. Olson, L. J., Peterson, F. C., Castonguay, A., Bohnsack, R. N., Kudo, M., Gotschall, R. R., Canfield, W. M., Volkman, B. F., and Dahms, N. M. (2010) Structural Basis for Recognition of Phosphodiester-containing Lysosomal Enzymes by the Cation-Independent Mannose 6-Phosphate Receptor. Proc. Natl. Acad. Sci. 107, 12493-12498 (Article highlighted in PNAS weekly series "In This Issue" Proc. Natl. Acad. Sci. 107, 12407-12408)
16. Castonguay, A., Lasanajak, Y., Song, X., Olson, L. J., Cummings, R. D., Smith, D.F., and Dahms, N. M. The Glycan Binding Properties of the Cation-Independent Mannose 6-Phosphate Receptor are Evolutionary Conserved. (2012) Glycobiology 22, 983-996 PMID: 22369936 PMCID: PMC3355666
17. Olson, L.J., Alculumbre, S.G., Peterson, F.C., Stigliano, I.D., Caramelo, J.J., Parodi, A.J., D'Allesio, C., and Dahms, N.M. Structure of the Lectin MRH Domain of Glucosidase II, an Enzyme that Regulates Glycoprotein Folding Quality Control in the Endoplasmic Reticulum. (2013) J. Biol. Chem. 288, 16460-16475. PMID: 23609449 PMCID: PMC3675582
18. Olson, L.J., Castonguay, A.C., Lasanajak, Y., Peterson, F.C. Cummings, R.D., Smith, D.F., and Dahms, N.M., Identification of a Fourth Mannose 6-Phosphate Binding Site in the Cation-Independent Mannose 6-Phosphate Receptor (2015) Glycobiology. PMID: 25573276 PMCID: PMC4410830
19. Olson L.J., Jensen D.R., Volkman B.F., Kim J.J., Peterson F.C, Gundry R.L., Dahms N.M, Bacterial Expression of the Phosphodiester-binding site of the Cation-independent Mannose 6-phosphate Receptor for crystallographic and NMR studies. Protein Expr Purif 2015 Jul;111:91-7 PMID: 25863146 PMCID: PMC4417374 04/12/2015
20. Olson L.J., Orsi R., Peterson F.C., Parodi A.J., Kim J.J., D'Alessio C., Dahms N.M., Crystal Structure and Functional Analyses of the Lectin Domain of Glucosidase II: Insights into Oligomannose Recognition. Biochemistry 2015 Jul 7;54(26):4097-111 PMID: 26062005
21. Olson L.J., Castonguay A.C., Lasanajak Y., Peterson F.C., Cummings R.D., Smith D.F., Dahms N.M., Identification of a Fourth Mannose 6-phosphate Binding Site in the Cation-Independent Mannose 6-Phosphate Receptor. Glycobiology. 2015; 25(6):591-606.
22. Olson L.J., Misra S.K., Ishihara M, Battaile, K.P., Grant, O., Sood, A., Woods, R.J., Kim, J.-J.P., Tiemeyer, M., Ren, G., Sharp, J.S., Dahms, N.M. Allosteric Regulation of Lysosomal Enzyme Recognition by the Cation-Independent Mannose 6-Phosphate Receptor. BioRxiv February 2020. doi: https://doi.org/10.1101/2020.02.21.959957
 
Abstracts
1. Draeger-Olson, L.J. and Mullen, G.P. (1993) Structural Properties of the c-Myc-92 family of H1 Peptides and their Inhibition of GST-c-Myc-92 DNA binding. 22nd Steenbock Symposium on Protein-Nucleic Acid Interactions, Madison, WI.
2. Olson, L.J., Zhang, J., Lee, Y. C., Dahms, N. M., and Kim, J.-J. P. (1999) Structure of a Functional Cation-Dependent Mannose 6-Phosphate Receptor Bound to Pentamannosyl Phosphate. ACA #W0190
3. Kim, J.-J. P., Olson, L. J., Zhang, J., and Dahms, N. M. (2001) The Twists and Turns of the CD-MPR in the Ligand-Bound Versus Ligand-Free State. Glycobiology 11, 880, Abstract # 51
4. Olson, L.J., Hindsgaul, O., Dahms, N. M. and Kim, J.-J. P. (2007) Structural Insights into the Mechanism of pH-Dependent Ligand Binding by the 46kDa Cation-Dependent Mannose 6-Phosphate Receptor. Glycobiology 17, 1270, Abstract # 261
5. Olson, L.J., Peterson, F. C., Kudo, M., Gotschall, R. R., Canfield, W. M., Volkman, B. F., and Dahms, N. M. (2008) Solution Structure of Domain 5 of the 300 kDa Cation-Independent Mannose 6-Phosphate Receptor Reveals Mode of Phosphodiester Recognition. Glycobiology 18, 993 Abstract #216
6. Bohnsack, R. N., Olson, L.J., Kudo, M., Gotschall, R. R., Canfield, W. M., and Dahms, N. M. (2008) Interaction of the 300 kDa Cation-Independent Mannose 6-Phosphate Receptor with Lysosomal Enzymes containing Phosphodiesters Glycobiology 18, 944 Abstract # 20
7. Dahms, N. M., Bohnsack, R. N., Song, X., Olson, L.J., Kudo, M., Gotschall, R. R., Canfield, W. M., Cummings, R., and Smith, D. (2009) Cation-Independent Mannose 6-Phosphate Receptor: A Composite of Distinct Phosphomannosyl Binding Sites Glycobiology 19, 1293 Abstract # 18
8. Song, X., Lasanajak, Y., Olson, L.J., Boonen, M., Dahms, N. M., Olson, L. J., Kornfeld, S., Cummings, R., and Smith, D. (2009) Glycan Microarray Analysis of P-Type Lectins Reveals Distinct Phospho-Mannose Glycan Recognition Glycobiology 19, 1353 Abstract # 221
9. Castonguay, A., Olson, L.J., Bohnsack, R. N., Kudo, M., Gotschall, R. R., Canfield, W. M., and Dahms, N. M. (2010) Man-P-GlcNAc Recognition by Domain 5 of the Cation-Independent Mannose 6-Phosphate Receptor. FASEB J. 24:lb106
10. Castonguay, A., Olson, L.J., Bohnsack, R. N., and Dahms, N. M. (2010) Characterization of the Carbohydrate Binding Properties of the Danio rerio Cation-Independent Mannose 6-Phosphate Receptor. Glycobiology 20, 1516, Abstract #207
11. Olson, L.J., Orsi, R., Alculumbre, S.G., Peterson, F.C., Stigliano, I.D., Parodi, A., D'Alessio, C., and Dahms, N.M. (2013) . Structure of the MRH Domain of Glucosidase II, a Key Enzyme in Glycoprotein Folding Quality Control. Glycobiology Gordon Conference
12. Orsi, R., Stigliano, I.D., Olson, L.J., Peterson, F.C., Alculumbre, S.G., Dahms, N.M., Parodi, A.J., D'Alessio, C. (2014) Glucosidase II: Quality Control from Folding Glycoprotein to the Structure of its domain lectin MRH. 1st Argentinean Symposium Glycobiology (GlycoAR)
13. Orsi, R., Olson, L.J., Peterson, F.C., Kim, J.J.P., Parodi, Dahms, N.M.,A.J., D'Alessio, C. (2015) N-Glycosylation and Glycoprotein's Fate in the Fission Yeast Secretory Pathway. Latin-American Society for Glycobiology
14. Bohnsack, R., Miller, J., Twining S., Olson, L.J. and Dahms, N.M. (2017) Cation-Independent Mannose 6-Phosphate Receptor Interacts with Several Components of the Plasminogen Activaton System. ASBMB.
15. Misra, S., Olson, L., Dahms, N.M., Sharp, J.S. (2019) Determination of ligand and pH-induced Conformation Changes in the Cation-Independent Mannose 6-Phosphate Receptor by Fast Photochemical Oxidation of Proteins. ASMS.
 
Database, Video, or Other Research/Clinical Contributions
1. 1C39, Structure of Cation-Dependent Mannose 6-Phosphate Receptor Bound to Pentamannosyl Phosphate, 7/25/1999, Olson, L.J., Zhang, J., Lee, Y.C., Dahms, N.M., Kim, J.J.-P.
2. 1KEO, Twists and Turns of the CD-MPR: Ligand-bound versus Ligand-Free Receptor, 11/16/2001, Olson, L.J., Zhang, J., Dahms, N.M., Kim, J.J.
3. 1SYO, N-terminal 3 domains of the CI-MPR bound to Mannose 6-Phosphate, 4/1/2004, Olson, L.J., Dahms, N.M., Kim, J.-J.P.
4. 1SZ0, N-terminal 3 domains of the CI-MPR bound to Mannose 6-Phosphate, 4/1/2004, Olson, L.J., Dahms, N.M., Kim, J.-J.P.
5. 1Q25, Crystal structure of N-terminal 3 domains of CI-MPR, 6/29/2004, Olson, L.J., Dahms, N.M., Kim, J.-J.P.
6. 2RLB, Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P in Absence of Mn, 10/18/2007, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P.
7. 2RL7, Crystal Structure Cation-Dependent Mannose 6-Phosphate receptor at pH 4.8, 10/18/2007, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P.
8. 2RL8, Crystal Structure Cation-Dependent Mannose 6-Phosphate receptor at pH 6.5 Bound to M6P, 10/18/2007, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P.
9. 2RL9, Crystal Structure Cation-Dependent Mannose 6-Phosphate receptor at pH 6.5 Bound to Trimannoside, 10/18/2007, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P.
10. 3CY4, Crystal Structure Cation-Dependent Mannose 6-Phosphate receptor at pH 7.4, 4/25/2008, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P.
11. 3K41, Crystal Structure of sCD-MPR mutant E19Q/K137M bound to Man-6-P, 10/5/2009, Olson, L.J., Sun, G., Bohnsack, R.N., Peterson, F.C., Dahms, N.M., Kim, J.J.P.
12. 3K42, Crystal Structure of sCD-MPR mutant E19Q/K137M at pH 7.0, 10/5/2009, Olson, L.J., Sun, G., Bohnsack, R.N., Peterson, F.C., Dahms, N.M., Kim, J.J.P.
13. 3K43, Crystal Structure of sCD-MPR mutant E19Q/K137M at pH 6.5, 10/5/2009, Olson, L.J., Sun, G., Bohnsack, R.N., Peterson, F.C., Dahms, N.M., Kim, J.J.P.
14. 2KVA, Solution Structure of the CI-MPR Ligand -Free Domain 5, 3/10/10, Olson, L.J., Peterson, F.C., Volkman, B.F., Dahms, N.M.
15. 2KVB, Solution Structure of CI-MPR Domain 5 bound to N-Acetylglucosaminyl 6-Phosphomethylmannoside, 3/10/2010, Olson, L.J., Peterson, F.C., Volkman, B.F., Dahms, N.M.
16. 2LVX, MRH Domain of Glucosidase II beta Subunit from S. pombe, 7/12/2012, Dahms, N.M., Olson, L.J., Peterson, F.C.
17. 4XQM, Crystal Structure of the MRH Domain of Glucosidase II beta Bound to Mannose, 1/19/15, Olson, L.J., Dahms, N.M., and Kim, J.J.-P
18. 2N1H, Solution Structure of the GBII-beta MRH domain W409A Point Mutant, 4/1/15, Olson, L.J., Peterson, F.C., Dahms, N.M.
19. 6P8I, N-terminal 5 Domains of IGFIIR, 6/7/19, Olson, L.J. and Dahms, N.M.
20. 6V02, N-terminal 5 Domains of CI-MPR, 11/18/19, Olson, L.J. and Dahms, N.M.