CURRICULUM VITAE
Linda J. Olson PhD
Linda J. Olson PhD
Assistant Professor
Department of Pharmacology and Toxicology
Department of Pharmacology and Toxicology
OFFICE ADDRESS: |
Translational and Biomedical Research Center |
8701 Watertown Plank Rd |
Milwaukee, WI 53226 |
EDUCATION: |
1983 - 1987 BA - Chemistry, Lawrence University, Appleton, WI |
1989 - 1991 Doctoral student with Dr. Stephen W. Ragsdale, UW-Milwaukee, Milwaukee, WI |
1991 - 1994 Doctoral student with Gregory P. Mullen, UW-Milwaukee, Milwaukee, WI |
1994 - Present Ph.D. Chemistry, Dissertation title: Studies of Eukaryotic DNA binding Proteins. Part A: Structure Function Studies on c-Myc Derived Peptides. Part B: Preliminary Studies of the Structure of DNA Polymerase . UW, Milwaukee, WI |
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS: |
1987 - 1989 Secondary school teacher of chemistry, physics, general science, St. Lawrence Seminary High School |
1988 - 1989 Science Department Chairperson, St. Lawrence Seminary High School |
1989 - 1992 Graduate research assistant, The University of Wisconsin-Milwaukee (advisor: Stephen Ragsdale) |
1992 - Present Instructor, Mathematics, Med-Prep Program, The University of Wisconsin-Milwaukee |
1992 - 1994 Graduate teaching assistant, The University of Wisconsin-Milwaukee (advisor: Dr. Gregory Mullen) |
1994 - 1997 Postdoctoral Fellow, Dept. Pharmacology, Medical College of Wisconsin, Milwaukee, WI |
1994 - 1997 Postdoctoral fellow, Dept. of Pharmacology, Medical College of Wisconsin (advisor: Dr. Drewett) |
1994 - 1995 Lecturer, Survey of Biochemistry, The University of Wisconsin-Milwaukee |
1997 - 1999 Postdoctoral Fellow, Dept. Biochemistry, Medical College of Wisconsin, Milwaukee, WI |
1997 - 1999 Postdoctoral fellow, Dept. of Biochemistry, Medical College of Wisconsin (advisors: Drs. Dahms and Kim) |
1999 - 2000 Senior Research Associate, Dept. of Biochemistry, Medical College of Wisconsin (advisors: Drs. Dahms and Kim) |
2000 - 2016 Research Scientist II, Dept. of Biochemistry, Medical College of Wisconsin (advisors: Drs. Dahms and Kim) |
2014 - Present X-ray facility manager, Dept. of Biochemistry, Medical College of Wisconsin |
2016 - Present Research Track Assistant Professor |
Positions and Employment: |
AWARDS AND HONORS: |
1983 - 1984 Lucia R. Briggs Scholarship, Lawrence University, Appleton, WI |
1986 - 1987 Dean's List, Lawrence University, Appleton, WI |
2016 - 2017 Graduate Student Award for Outstanding Teacher of the Year, Medical College of Wisconsin |
2017 - 2018 Outstanding Graduate School Teacher, Medical College of Wisconsin |
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES: |
1989 - 1994 Member (American Association for the Advancement of Science) |
1991 - Present American Chemical Society (Member) |
1999 - Present Member (American Crystallographic Association) |
2019 - Present Member (Society for Glycobiology) |
Source: American Heart Association |
- 1997 Dates: 1997 - 1999 |
Title: Natriuretic Peptide Receptors and Aldosterone |
Role: Post-Doctoral Fellow in Laboratory of Dr. James G. Drewett |
Direct Funds: $41 (000) |
Declined due to change of Mentor due to PI relocating |
Title: Postdoctoral Fellowship Grant (1 F32 HL09697-01A1) |
Source: Department of Health and Human Services |
- 1997 Dates: 1997 - 1999 |
Title: Natriuretic Peptide Receptors and Aldosterone |
Role: Post-Doctoral Fellow in Laboratory of Dr. James G. Drewett |
Direct Funds: predetermined |
Seminar Structure of a Functional Cation-Dependent Mannose 6-Phosphate Receptor Bound to Pentamannosyl Phosphate, American Crystallographic Association, Buffalo NY (1999) |
Current |
Title: Identification of Novel Glucosidase II Inhibitors to Inhibit Breast Cancer Metastasis |
Source: MCW Cancer Center Pilot Award |
Dates: July 2017-present |
Role: PI |
Direct Funds: $50 (000) |
Title: Preliminary Studies on the Structure and Function of Human Salt Inducible Kinase 2 |
Source: Research Affairs Committee (Medical College of Wisconsin) |
Dates: August 2018-present |
Role: PI |
Direct Funds: $ 25 (000) |
INVITED PRESENTATIONS: |
Local |
Seminar, Insights into the Ligand Binding Specificity of the Cation-Dependent Mannose 6-phosphate Receptor. Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (March 1999) |
Seminar All in a Day's Work, Structural Insights into the Functioning of CD-MPR, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (May 2001) |
Seminar Crystal Structure of the N-terminal Three Domains of the CI-MPR, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (April 2003) |
Seminar My Continuing Journey with Mannose 6-Phosphate Receptor and Lysosomal Enzyme Trafficking, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (April 2007) |
Seminar Glucosidase II : the Importance of Sugars in the Folding of Proteins in the ER, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (May 2012) |
Seminar The Multi-functionality of the IGF2 Receptor: a Biophysical Study of Domains 1-5, Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI (April 2019.) |
National |
Title: Postdoctoral Fellowship Grant (97-F-Post-02) |
Peer Review |
Prior |
RESEARCH GRANTS/AWARDS: |
Declined due to change of Mentor due to PI relocating |
BIBLIOGRAPHY |
Refereed Journal Publications/Original Papers |
1. Draeger-Olson, L. J. and Mullen G. P. Interaction of the bHLH-Zip Domain of c-Myc with H1-Type Peptides. (1994) J. Biol. Chem. 269,1785-1793. PMID: 8294427 |
2. Olson L. J., Lowe D. G., and Drewett J. G. Novel natriuretic peptide receptor/guanylyl cyclase A-selective agonist inhibits angiotensin II- and forskolin-evoked aldosterone synthesis in a human zona glomerulosa cell line. (1996) Mol Pharmacol. 50, 430-435. PMID: 8700153 |
3. Olson, L. J., Knych, Jr., E. T., Herzig, T. C., and Drewtt, J. G. Selective Guanylyl Cyclase Inhibitor Reverses Nitric Oxide-Induced Vasorelaxation. (1997) Hypertension 29, 254-261. PMID: 9039111 |
4. Olson, L.J., Ho, B.Y, Cashdollar, L.W., and Drewett, J.G. Functionally active catalytic domain is essential for guanylyl cyclase-linked receptor mediated inhibition of human aldosterone synthesis. (1998) Mol Pharmacol. 54, 761-769. PMID: 9804611 |
5. Olson, L. J., Zhang, J., Lee, Y. C., Dahms, N. M., and Kim, J.-J. P. Structural Basis for Recognition of Phosphorylated High-Mannose Oligosaccharides by the Cation-Dependent Mannose 6-Phosphate Receptor. (1999) J. Biol. Chem. 274, 29889-29896. |
6. Olson, L. J., Hancock, M. K., Hubbard, P., Dix, D., Kim, J.-J. P., and Dahms, N. M. Mutational Analysis of the Binding Site Residues of the Bovine Cation-Dependent Mannose 6-Phosphate Receptor. (1999) J. Biol. Chem. 274, 36905-36911. |
7. Olson, L. J., Zhang, J., Dahms, N. M., and Kim, J.-J. P. Twists and Turns of the Cation-Dependent Mannose 6-Phosphate Receptor: Ligand-Bound versus Ligand-Free Receptor. (2002) J. Biol. Chem. 277, 10156-10161. |
8. Olson, L. J., Yammani, R., Dahms, N. M., and Kim, J.-J. P. (2004) Structure of the uPAR, Plasminogen, and Sugar Binding Sites of the 300 kDa Mannose 6-Phosphate Receptor. EMBO J 23, 2019-2028. |
9. Olson, L. J., Dahms, N. M., and Kim, J.-J. P. The N-Terminal Carbohydrate Recognition Site of the Cation-Independent Mannose 6-Phosphate Receptor. (2004) J. Biol. Chem. 279, 34000-34009. |
10. Olson, L. J., Hindsgaul, O., Dahms, N. M., and Kim, J.-J. P. Structural Insights into the Mechanism of pH dependent Ligand Binding and Release by the Cation-Dependent Mannose 6-Phosphate Receptor. (2008) J. Biol. Chem. 283, 10124-10134 |
11. Song, X., Lasanajak, Y., Olson, L. J., Boonen, M., Dahms, N. M., Kornfeld, S., Cummings, R. D., and Smith, D. F. (2009) Glycan Microarray Analysis of P-Type Lectins Reveals Distinct Phospho-Mannose Glycan Recognition J. Biol. Chem. 284, 35201-35214 |
12. Bohnsack, R. N., Song, X., Olson, L. J., Kudo, M., Gotschall, R. R., Canfield, W. M., Cummings, R. D., Smith, D., F., and Dahms, N. M. Cation-Independent Mannose 6-Phosphate Receptor: A Composite of Distinct Phosphomannosyl Binding Sites J. Biol. Chem. 284, 35215-35226 |
13. Olson, L. J., Sun, G., Bohnsack, R. N., Peterson, F. C., Dahms, N. M.*, and Kim, J.-J. P.* (2010) Intermonomer Interactions are Essential for Lysosomal Enzyme Binding by the Cation-Dependent Mannose 6-Phosphate Receptor. Biochemistry 49, 236-246. {Manuscript chosen to be highlighted on the journal's home page} |
14. Bohnsack, R. N., Patel, M., Olson, L. J., Twinning, S. S., and Dahms, N. M. (2010) Residues Essential for Plasminogen Binding by the Cation-Independent Mannose 6-Phosphate Receptor. Biochemistry 49, 635-644 |
15. Olson, L. J., Peterson, F. C., Castonguay, A., Bohnsack, R. N., Kudo, M., Gotschall, R. R., Canfield, W. M., Volkman, B. F., and Dahms, N. M. (2010) Structural Basis for Recognition of Phosphodiester-containing Lysosomal Enzymes by the Cation-Independent Mannose 6-Phosphate Receptor. Proc. Natl. Acad. Sci. 107, 12493-12498 (Article highlighted in PNAS weekly series "In This Issue" Proc. Natl. Acad. Sci. 107, 12407-12408) |
16. Castonguay, A., Lasanajak, Y., Song, X., Olson, L. J., Cummings, R. D., Smith, D.F., and Dahms, N. M. The Glycan Binding Properties of the Cation-Independent Mannose 6-Phosphate Receptor are Evolutionary Conserved. (2012) Glycobiology 22, 983-996 PMID: 22369936 PMCID: PMC3355666 |
17. Olson, L.J., Alculumbre, S.G., Peterson, F.C., Stigliano, I.D., Caramelo, J.J., Parodi, A.J., D'Allesio, C., and Dahms, N.M. Structure of the Lectin MRH Domain of Glucosidase II, an Enzyme that Regulates Glycoprotein Folding Quality Control in the Endoplasmic Reticulum. (2013) J. Biol. Chem. 288, 16460-16475. PMID: 23609449 PMCID: PMC3675582 |
18. Olson, L.J., Castonguay, A.C., Lasanajak, Y., Peterson, F.C. Cummings, R.D., Smith, D.F., and Dahms, N.M., Identification of a Fourth Mannose 6-Phosphate Binding Site in the Cation-Independent Mannose 6-Phosphate Receptor (2015) Glycobiology. PMID: 25573276 PMCID: PMC4410830 |
19. Olson L.J., Jensen D.R., Volkman B.F., Kim J.J., Peterson F.C, Gundry R.L., Dahms N.M, Bacterial Expression of the Phosphodiester-binding site of the Cation-independent Mannose 6-phosphate Receptor for crystallographic and NMR studies. Protein Expr Purif 2015 Jul;111:91-7 PMID: 25863146 PMCID: PMC4417374 04/12/2015 |
20. Olson L.J., Orsi R., Peterson F.C., Parodi A.J., Kim J.J., D'Alessio C., Dahms N.M., Crystal Structure and Functional Analyses of the Lectin Domain of Glucosidase II: Insights into Oligomannose Recognition. Biochemistry 2015 Jul 7;54(26):4097-111 PMID: 26062005 |
21. Olson L.J., Castonguay A.C., Lasanajak Y., Peterson F.C., Cummings R.D., Smith D.F., Dahms N.M., Identification of a Fourth Mannose 6-phosphate Binding Site in the Cation-Independent Mannose 6-Phosphate Receptor. Glycobiology. 2015; 25(6):591-606. |
22. Olson L.J., Misra S.K., Ishihara M, Battaile, K.P., Grant, O., Sood, A., Woods, R.J., Kim, J.-J.P., Tiemeyer, M., Ren, G., Sharp, J.S., Dahms, N.M. Allosteric Regulation of Lysosomal Enzyme Recognition by the Cation-Independent Mannose 6-Phosphate Receptor. BioRxiv February 2020. doi: https://doi.org/10.1101/2020.02.21.959957 |
Abstracts |
1. Draeger-Olson, L.J. and Mullen, G.P. (1993) Structural Properties of the c-Myc-92 family of H1 Peptides and their Inhibition of GST-c-Myc-92 DNA binding. 22nd Steenbock Symposium on Protein-Nucleic Acid Interactions, Madison, WI. |
2. Olson, L.J., Zhang, J., Lee, Y. C., Dahms, N. M., and Kim, J.-J. P. (1999) Structure of a Functional Cation-Dependent Mannose 6-Phosphate Receptor Bound to Pentamannosyl Phosphate. ACA #W0190 |
3. Kim, J.-J. P., Olson, L. J., Zhang, J., and Dahms, N. M. (2001) The Twists and Turns of the CD-MPR in the Ligand-Bound Versus Ligand-Free State. Glycobiology 11, 880, Abstract # 51 |
4. Olson, L.J., Hindsgaul, O., Dahms, N. M. and Kim, J.-J. P. (2007) Structural Insights into the Mechanism of pH-Dependent Ligand Binding by the 46kDa Cation-Dependent Mannose 6-Phosphate Receptor. Glycobiology 17, 1270, Abstract # 261 |
5. Olson, L.J., Peterson, F. C., Kudo, M., Gotschall, R. R., Canfield, W. M., Volkman, B. F., and Dahms, N. M. (2008) Solution Structure of Domain 5 of the 300 kDa Cation-Independent Mannose 6-Phosphate Receptor Reveals Mode of Phosphodiester Recognition. Glycobiology 18, 993 Abstract #216 |
6. Bohnsack, R. N., Olson, L.J., Kudo, M., Gotschall, R. R., Canfield, W. M., and Dahms, N. M. (2008) Interaction of the 300 kDa Cation-Independent Mannose 6-Phosphate Receptor with Lysosomal Enzymes containing Phosphodiesters Glycobiology 18, 944 Abstract # 20 |
7. Dahms, N. M., Bohnsack, R. N., Song, X., Olson, L.J., Kudo, M., Gotschall, R. R., Canfield, W. M., Cummings, R., and Smith, D. (2009) Cation-Independent Mannose 6-Phosphate Receptor: A Composite of Distinct Phosphomannosyl Binding Sites Glycobiology 19, 1293 Abstract # 18 |
8. Song, X., Lasanajak, Y., Olson, L.J., Boonen, M., Dahms, N. M., Olson, L. J., Kornfeld, S., Cummings, R., and Smith, D. (2009) Glycan Microarray Analysis of P-Type Lectins Reveals Distinct Phospho-Mannose Glycan Recognition Glycobiology 19, 1353 Abstract # 221 |
9. Castonguay, A., Olson, L.J., Bohnsack, R. N., Kudo, M., Gotschall, R. R., Canfield, W. M., and Dahms, N. M. (2010) Man-P-GlcNAc Recognition by Domain 5 of the Cation-Independent Mannose 6-Phosphate Receptor. FASEB J. 24:lb106 |
10. Castonguay, A., Olson, L.J., Bohnsack, R. N., and Dahms, N. M. (2010) Characterization of the Carbohydrate Binding Properties of the Danio rerio Cation-Independent Mannose 6-Phosphate Receptor. Glycobiology 20, 1516, Abstract #207 |
11. Olson, L.J., Orsi, R., Alculumbre, S.G., Peterson, F.C., Stigliano, I.D., Parodi, A., D'Alessio, C., and Dahms, N.M. (2013) . Structure of the MRH Domain of Glucosidase II, a Key Enzyme in Glycoprotein Folding Quality Control. Glycobiology Gordon Conference |
12. Orsi, R., Stigliano, I.D., Olson, L.J., Peterson, F.C., Alculumbre, S.G., Dahms, N.M., Parodi, A.J., D'Alessio, C. (2014) Glucosidase II: Quality Control from Folding Glycoprotein to the Structure of its domain lectin MRH. 1st Argentinean Symposium Glycobiology (GlycoAR) |
13. Orsi, R., Olson, L.J., Peterson, F.C., Kim, J.J.P., Parodi, Dahms, N.M.,A.J., D'Alessio, C. (2015) N-Glycosylation and Glycoprotein's Fate in the Fission Yeast Secretory Pathway. Latin-American Society for Glycobiology |
14. Bohnsack, R., Miller, J., Twining S., Olson, L.J. and Dahms, N.M. (2017) Cation-Independent Mannose 6-Phosphate Receptor Interacts with Several Components of the Plasminogen Activaton System. ASBMB. |
15. Misra, S., Olson, L., Dahms, N.M., Sharp, J.S. (2019) Determination of ligand and pH-induced Conformation Changes in the Cation-Independent Mannose 6-Phosphate Receptor by Fast Photochemical Oxidation of Proteins. ASMS. |
Database, Video, or Other Research/Clinical Contributions |
1. 1C39, Structure of Cation-Dependent Mannose 6-Phosphate Receptor Bound to Pentamannosyl Phosphate, 7/25/1999, Olson, L.J., Zhang, J., Lee, Y.C., Dahms, N.M., Kim, J.J.-P. |
2. 1KEO, Twists and Turns of the CD-MPR: Ligand-bound versus Ligand-Free Receptor, 11/16/2001, Olson, L.J., Zhang, J., Dahms, N.M., Kim, J.J. |
3. 1SYO, N-terminal 3 domains of the CI-MPR bound to Mannose 6-Phosphate, 4/1/2004, Olson, L.J., Dahms, N.M., Kim, J.-J.P. |
4. 1SZ0, N-terminal 3 domains of the CI-MPR bound to Mannose 6-Phosphate, 4/1/2004, Olson, L.J., Dahms, N.M., Kim, J.-J.P. |
5. 1Q25, Crystal structure of N-terminal 3 domains of CI-MPR, 6/29/2004, Olson, L.J., Dahms, N.M., Kim, J.-J.P. |
6. 2RLB, Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P in Absence of Mn, 10/18/2007, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P. |
7. 2RL7, Crystal Structure Cation-Dependent Mannose 6-Phosphate receptor at pH 4.8, 10/18/2007, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P. |
8. 2RL8, Crystal Structure Cation-Dependent Mannose 6-Phosphate receptor at pH 6.5 Bound to M6P, 10/18/2007, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P. |
9. 2RL9, Crystal Structure Cation-Dependent Mannose 6-Phosphate receptor at pH 6.5 Bound to Trimannoside, 10/18/2007, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P. |
10. 3CY4, Crystal Structure Cation-Dependent Mannose 6-Phosphate receptor at pH 7.4, 4/25/2008, Olson, L.J., Hindsgaul, O., Dahms, N.M., Kim, J.-J.P. |
11. 3K41, Crystal Structure of sCD-MPR mutant E19Q/K137M bound to Man-6-P, 10/5/2009, Olson, L.J., Sun, G., Bohnsack, R.N., Peterson, F.C., Dahms, N.M., Kim, J.J.P. |
12. 3K42, Crystal Structure of sCD-MPR mutant E19Q/K137M at pH 7.0, 10/5/2009, Olson, L.J., Sun, G., Bohnsack, R.N., Peterson, F.C., Dahms, N.M., Kim, J.J.P. |
13. 3K43, Crystal Structure of sCD-MPR mutant E19Q/K137M at pH 6.5, 10/5/2009, Olson, L.J., Sun, G., Bohnsack, R.N., Peterson, F.C., Dahms, N.M., Kim, J.J.P. |
14. 2KVA, Solution Structure of the CI-MPR Ligand -Free Domain 5, 3/10/10, Olson, L.J., Peterson, F.C., Volkman, B.F., Dahms, N.M. |
15. 2KVB, Solution Structure of CI-MPR Domain 5 bound to N-Acetylglucosaminyl 6-Phosphomethylmannoside, 3/10/2010, Olson, L.J., Peterson, F.C., Volkman, B.F., Dahms, N.M. |
16. 2LVX, MRH Domain of Glucosidase II beta Subunit from S. pombe, 7/12/2012, Dahms, N.M., Olson, L.J., Peterson, F.C. |
17. 4XQM, Crystal Structure of the MRH Domain of Glucosidase II beta Bound to Mannose, 1/19/15, Olson, L.J., Dahms, N.M., and Kim, J.J.-P |
18. 2N1H, Solution Structure of the GBII-beta MRH domain W409A Point Mutant, 4/1/15, Olson, L.J., Peterson, F.C., Dahms, N.M. |
19. 6P8I, N-terminal 5 Domains of IGFIIR, 6/7/19, Olson, L.J. and Dahms, N.M. |
20. 6V02, N-terminal 5 Domains of CI-MPR, 11/18/19, Olson, L.J. and Dahms, N.M. |