Medical College of Wisconsin
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Young-In Chi PhD
Assistant Professor
Department of Surgery
Division of Administration

Medical Education Building
8701 Watertown Plank Rd
Milwaukee, WI 53226

08/30/1984 - 05/08/1988 BA, Central Methodist College, Fayette, MO
08/25/1988 - 12/17/1994 PhD, Purdue University, West Lafayette, IN

10/01/1994 - 01/31/1998 Postdoctoral Fellow, Department of Chemistry, University of California, Berkeley, CA 94720
02/01/1998 - 08/30/2003 Research Fellow, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215

09/1980 - 12/1983 Republic of Korea Army

09/2003 - 08/2011 Assistant Professor, Department of Molecular and Cellular Biochemistry, University of Kentucky School of Medicine, Lexington, KY
09/2011 - 06/2017 Assistant Professor, Hormel Institute, University of Minnesota, Austin, MN
07/2017 - 12/2018 Research Professor, Department of Internal Medicine, Kyungpook National University School of Medicine, Deagu, Republic of Korea
02/2018 - Present Assistant Professor, Department of Surgery, Division of Research, The Medical College of Wisconsin, Milwaukee, WI






09/1985 - 05/1986 Bernice Fuller Connell Scholarship
09/1986 - 05/1988 Kappa Mu Epsilon Honorary Mathematics Society
05/1988 William L. Spencer Award
05/1988 Sigma Epsilon Pi Academic Award
05/1988 Magna cum laude graduate, Central Methodist College
09/1990 - 12/1994 David Ross Research Fellowship
01/1999 - 12/2000 Juvenile Diabetes Research Foundation Postdoc Fellowship
01/2001 - 08/2003 Mary K. Iacocca Fellowship
07/2001 American Diabetes Association Annual Meeting Travel Grant Award
09/2004 - 08/2011 Charles T. Wethington Research Award
07/2008 - 06/2013 American Diabetes Association Career Development Award

American Society of Human Genetics
American Crystallographic Association
Korean-American Scientists and Engineers Association
Biophysical Society
American Diabetes Association
Protein Society

05/2006 Outside Examiner for Tao Liu's Final Examination Committee (College of Pharmacy, Univ. of Kentucky)
06/2006 - 05/2008 Thesis Committee for Brandon Adkins (Department of Molecular and
08/2006 - 08/2009 University of Kentucky Radiation Safety Committee
01/2007 - 12/2011 SER-CAT alternative board member and institution representative
02/2007 Outside Examiner for Paul Kiptoo's Final Examination Committee (College of Pharmacy, Univ. of Kentucky)
03/2011 Outside Examiner for Lorenzo Federico's Final Examination Committee (College of Pharmacy, Univ. of Kentucky)
09/2015 Outside Examiner for Carlos Ramirez-Mondragon's Preliminary Written Examination (BICB, Univ. of Minnesota)

Peer Review
Mechanism of Transcriptional Control by Two Diabetes Gene Products (Center of Biomedical Research Excellence in the Molecular Basis of Human Diseases)
NIH/NCRR (P20-RR020171-01)
Lou B. Hersh)
07/01/2004 - 06/30/2008
Direct Funds:
Beta-cell Master Regulator
Juvenile Diabetes Research Foundation (JDRF) Regular Research Grant (1-2004-506)
Principal Investigator
09/01/2004 - 08/31/2007
Direct Funds:
Structural and functional studies of HNF1alpha and HNF4alpha
American Diabetes Association (ADA) Career Development Award (7-08-CD-03)
Principal Investigator
07/01/2008 - 12/31/2013
Direct Funds:
Identification of HNF1alpha and HNF4alpha-mediated regulators of insulin secretion in beta-cells
Kentucky Diabetes Research Board (KDRB) Pilot Project (KDR-PP09-09)
Principal Investigator
07/01/2008 - 06/30/2010
Direct Funds:
Inhibition of ATGL-mediated lipolysis by G0S2
NIH/NIDDK (1R01DK089178-01)
07/01/2010 - 06/30/2012
Direct Funds:
$1,250,000 (total for all 5 years for PI, Jun Liu)
Molecular basis of auto-repression and its defect caused by diabetes mutations in HNF4alpha
Grant-in-Aid Bridge Fund, University of Minnesota
Principal Investigator
09/01/2014 - 06/30/2015
Direct Funds:
Determine the functional role of Dub3 in breast cancer progression and metastasis
NIH/NCI (R01CA188118)
04/01/2015 - 03/31/2017
Direct Funds:
$1,250,000 (total for all 5 years for PI, Binhua Zhou)
Structural characterization of Dub3/ubiquitin interactions for targeted therapies against basal-like breast cancers
Paint the town pink (PTTP) research fund
Principal Investigator
12/01/2015 - 11/30/2017
Direct Funds:
Structure-guided optimization of diabetes therapeutic compounds
Brain Pool, National Research Foundation of Korea
Invited Scientist
01/01/2018 - 12/31/2018
Direct Funds:
Molecular targeting of HNF4alpha repressive states
American Diabetes Association (ADA) Innovative Basic Science Award (1-18-IBS-291) (relinquished)
Principal Investigator
07/01/2018 - 06/30/2021
Direct Funds:
Non-Peer Review
Major Equipment Grant for the Isothermal Titration Calorimetry (VP-ITC)
University of Kentucky
Principal Investigator
Direct Funds:

September 13, 2012 Biomedical Informatics and Computational Biology (BICB) program, University of Minnesota, Rochester, Minnesota
October 22, 2015 Hormel Institute Research Seminar, University of Minnesota
December 14, 2011 Epigenetic and Chromatin Dynamics Lab, Mayo Clinic, Rochester, Minnesota
October 31, 2007 Nutritional Sciences Seminar Series, Graduate Center for
Nutritional Sciences, University of Kentucky
February 3, 2006 Toxicology Seminar Series, Graduate Center for Toxicology, University of Kentucky
April 3, 2013 International Conference of RNA Nanotechnology and Therapeutics, University of Kentucky
February 21, 2011 Nanobiomedical Center, College of Engineering and College of Medicine, University of Cincinnati
September 13, 2010 Department of Molecular Therapeutics, Scripps Florida
June 1, 2008 2008 Meeting of the American Crystallographic Association (ACA)*, Knoxville, TN, USA
October 14, 2005 Structural Biology Seminar, Berkeley Structural Genomics Center
University of California at Berkeley
January 21, 2004 Structural Biology Seminar, Biological Sciences Department
Purdue University
October 4, 2002 Department of Cell Biology Seminar, Harvard Medical School
February 4, 2002 Department of Molecular and Cell Biology, University of Connecticut
January 14, 2002 Department Seminar, Dept. of Microbiology, Molecular Biology and Biochemistry, University of Idaho
September 15, 2017 Department of Molecular Biology, Pusan National University Pusan, Korea
May 24, 2011 Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea
May 20, 2011 School of Life Sciences and Biotechonology, Korea University, Seoul, Korea
May 19, 2011 Department of Life Sciences, Sogang University, Seoul, Korea
August 30, 2008 XXI Congress and General Assembly of the International Union of
Crystallography (IUCr)*, Osaka, Japan
July 31, 2017 Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea

Graduate Student Education
Fall, 2013 Computation and Biology (BICB 8510)
Fall, 2005 - spring, 2011 Fundamentals of Biochemistry (BCH401G, Univ. of Kentucky)
Fall, 2007 - spring, 2008 Seminar in Biochemistry (BCH 618/619, Univ. of Kentucky)
Fall, 2004 - spring, 2008 Biochemistry and Cell Biology of Nucleic Acids (BCH 611, Univ. of Kentucky)
Fall, 2005 - spring, 2011 Structural Biology (BCH604, Univ. of Kentucky)

Undergraduate Students
Bartley Morris; undergraduate (January 2005 - December 2005)
Scott E. Collier; undergraduate (NSF-REU program; summer 2007)
Eric Gilland; undergraduate (Chellgren Fellow; May, 2009 - August, 2011)
Tim Horn; undergraduate (SURE program; summer 2012)
Graduate Students
PhD Students Advised
Brandon C. Adkins; graduate student, University of Kentucky, 2006 - 2008
Josh Lim; BICB graduate student, University of Minnesota, 2013 - 2014
PhD Committees
Brandon C. Adkins; PhD candidate, University of Kentucky, 2006 - 2008
Postdoctoral Students
Yun Li, Ph.D.; postdoc (June 2004 - December 2005)
Jianguo Liu, Ph.D.; postdoc (August 2004 - February 2006)
Peng Lu, Ph.D.; postdoc (January 2005 - February 2008)
Guangteng Wu, Ph.D.; postdoc/research associate (January 2006 - December 2011)
Gabriel J. Popa, Ph.D; postdoc (July 2008 - June 2009)
Eun Hee Han, Ph.D.; postdoc (September 2011 - August 2013)
Puja Singh, Ph.D.; postdoc (May 2012 - June 2017)
Clinical/Research Fellows
Eunkyung Yoo, MS; research assistant (July 2017 - December 2018)
Jinhak Jeong, BS; lab technician (July 2017 – December 2018)
James Endrizzi, PhD; research associate (March 2012 - August 2012)
Geun Bae Rha, MS; research associate (July 2007 - March 2010)
Seungmi Lee, BS; lab technician (July 2017 – December 2018)
Amy Gorman, MS; research associate (February 2004 - January 2007)
Shu-Ping Tung, MD/PhD; visiting Scholar (January 2014 - December 2014)

Refereed Journal Publications/Original Papers
1. Chi, Y.-I. and Lee, I.-K. (2021) Current overview of the structure and molecular mechanisms of a novel nuclear receptor HNF4alpha. Nucl. Recept. Signal. in preparation
2. Singh, P., Deng, Y. and Chi, Y.-I. (2021) Molecular details of human eIF4A1-eIF4G1 interaction that drives mRNA translation. Proc. Natl. Acad. Sci. U. S. A. in preparation
3. Chi, Y.-I., Stodola, T. J., De Assuncao, T. M., Levrence, E. N., Tripathi, S., Dsouza, N. R., Mathison, A. J., Basel, D. G., Volkman, B. F., Smith, B. C., Lomberk, G. Zimmermann, M. T. and Urrutia, R. (2021) Molecular mechanics and dynamic simulations of well-known Kabuki syndrome-associated KDM6A variants reveal putative mechanisms of dysfunction. Orphanet J. Rare Dis. 16:66.
4. Dou, L., Yan, F., Pang, J., Zheng, D., Li, D., Gao, L., Wang, L., Xu, Y., Shi, J., Wang, Q., Zhou, L., Shen, N., Singh, P., Wang, L., Li, Y., Gao, Y., Liu, T., Chen, C., Al-Kali, A., Litzow, M. R., Chi, Y.-I., Bode, A. M., Liu, C., Huang, H., Liu, D., Marcucci, G., Liu, S. and Yu, L. (2019) Protein lysine 43 methylation by EZH1 promotes AML1-ETO transcriptional repression in leukemia. Nature Commun. 10:5051.
5. Han, E., Singh, P., Kim, Y., Lee, I.-K. and Chi, Y.-I. (2019) ErbB3-binding protein 1 (EBP1) represses HNF4alpha-mediated transcription and insulin secretion in pancreatic beta-cells. J. Biol. Chem. 294:13983-13994.
6. Lee, D., Pagire, S., Pagire, H., Bae, E. J., Dighe, M., Kim, M., Lee, K. M., Jang, Y. K., Jaladi, A., Jung, K.-Y., Yoo, E. K., Gim, H. E., Lee, S. M., Choi, W.-I., Chi, Y.-I., Song, J.-S., Bae, M. A., Jeon, Y. H., Lee, G.-H., Liu, K.-H., Lee, T., Park, S., Jeon, J.-H., Lee, I. K. and Ahn, J. H. (2019) Discovery of novel pyruvate dehydrogenase kinase 4 inhibitors for potential oral treatment of metabolic diseases. J. Med. Chem. 62:575-88.
7. Singh, P., Tung, S.-P., Han, E. H., Lee, I.-K. and Chi, Y.-I. (2019) Dimerization defective MODY mutations of hepatocyte nuclear factor 4alpha. Mutat. Res. 814:1-6.
8. Jardetzky, T. S., Brown, J. H., Gorga, J. C., Stern, L. J., Urban, R.G., Chi, Y.-I., Stauffacher, C. V., Strominger, J. L. and Wiley, D. C. (2018) Pillars article: Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigen. Nature 368: 711-718. J. Immunol. 201:1819-26.
9. Singh, P., Han, E., Endrizzi, J. A., O'Brien, R. M. and Chi, Y.-I. (2017) Crystal structures reveal a new and novel FoxO1 binding site within the human glucose-6-phosphatase catalytic subunit 1 gene promoter. J. Struct. Biol. 198:54-64.
10. Wu, Y., Wang, Y., Lin, Y., Liu, Y., Wang, Y., Jia, J., Singh, P., Chi, Y.-I., Wang, C., Dong, D., Li, W., Tao, M., Napier, D., Shi, Q., Deng, J., Evers, B. M. and Zhou, B. P. (2017) Dub3 inhibition suppresses breast cancer invasion and metastasis by promoting snail1 degradation. Nature Commun. 8:14228.
11. Han, E., Gorman, A., Singh, P. and Chi, Y.-I. (2015) Repression of HNF1alpha-mediated transcription by amino-terminal enhancer of split (AES). Biochem. Biophys. Res. Commun. 468:14-20.
12. Rao, E., Singh, P., Li. Y., Zhang, Y., Chi, Y.-I., Suttles, J. and Li, B. (2015) Targeting epidermal fatty acid binding protein for treatment of experimental autoimmune encephalomyelitis. BMC Immunol. 16:28.
13. Rao, E., Singh, P., Zhai, X., Li, Y., Zhu, G., Zhang, Y., Hao, J., Chi, Y.-I., Brown, R., Cleary, M. P. and Li, B. (2015) Inhibition of tumor growth by a newly-identified activator for epidermal fatty acid binding protein. Oncotarget 6:7815-27.
14. Jung, S. K., Lee, M. H., Lim, D. Y., Kim, J. E., Singh, P., Lee, S. Y., Jeong, C. H., Lim, T. G., Chen, H., Chi, Y.-I., Kunda, J. K., Lee, N. H., Lee, C. C., Cho, Y. Y., Bode, A. M., Lee, K. W. and Dong, Z. (2014) Isoliquiritigenin induces apoptosis and inhibits xenograft tumor growth of human lung cancer cells by targeting both wildtype and L858R/T790M mutant EGFR. J. Biol. Chem. 289:35839-48.
15. Zhang, H., Endrizzi, J. A., Shu, Y., Haque, F., Sauter, C., Shlyakhtenko, L. S., Lyubchenko, Y., Guo, P. and Chi, Y.-I. (2013) Crystal structure of 3WJ core revealing divalent ion promoted thermostability and assembly of the phi29 hexameric motor pRNA. RNA 19:1226-37.
16. Lancman, J. J., Zvenigorodsky, N., Gate, K. P., Zhang, D., Solomon, K., Humphrey, R. K., Kuo, T., Setiawan, L., Verkade, H., Chi, Y.-I., Jhala, U. S., Wright, C. V., Stainier, D. Y. and Dong, P. D. (2013) Specification of hepatopancreas progenitors in zebrafish by hnf1b and wnt2bb. Development 140:2669-79.
17. Dong, C., Wu, Y., Wang Y., Wang C., Kang, T., Rychahou, P. G., Chi, Y.-I., Evers, B. M. and Zhou, B. P. (2013) Interaction with Suv39H1 is critical for snail-mediated E-cadherin repression in breast cancer. Oncogene 32:1351-62.
18. Han, E. H., Rha, G. B. and Chi, Y.-I. (2012) MED25 is a Mediator component of HNF4alpha-driven transcription leading to insulin secretion in pancreatic beta-cells. PLoS One 7:e44007.
19. Rha, G., Wu, G. and Chi, Y.-I. (2011) Probing the effect of MODY mutations near the coactivator binding pocket of HNF4alpha. Biosci. Rep. 31:411-9.
20. Robertson, M. P., Chi, Y.-I. and Scott, W. G. (2010) Solving novel RNA structures using only secondary structural fragments. Methods 52:168-72.
21. Lin, Y., Wu, Y., Li, J., Dong, C., Ye, X., Chi, Y.-I., Evers, B. M. and Zhou, B. P. (2010) The SNAG domain of Snail1 functions as a molecular hook for recruiting lysine-specific demethylase 1. EMBO J. 29:1803-16.
22. Yang, X., Lu, X., Lombes, M., Guerin, T. M., Rha, G., Chi, Y. -I., Smart, E. J. and Liu, J. (2010) The G0/G1 switch gene 2 regulates adipose lipolysis through association with adipose triglyceride lipase. Cell Metab. 11:194-205.
23. Rha, G., Wu, G., Shoelson, S. E. and Chi, Y.-I. (2009) Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. J. Biol. Chem. 284:35165-76.
24. Lu, P., Rha, G., Melikishvili, M., Wu, G., Adkins, B. C., Fried, M. G. and Chi, Y.-I. (2008) Structural basis of natural promoter recognition by a unique nuclear receptor HNF4alpha: Diabetes gene product. J. Biol. Chem. 283:33685-97.
25. Chi Y.-I., Martick, M., Lares, M., Kim, R., Scott, W. G. and Kim, S.-H. (2008) Capturing hammerhead ribozyme structures in action by modulating general base catalysis. PLoS Biology 6:e234.
26. Lu, P., Liu, J., Melikishvili, M., Fried, M. G. and Chi, Y.-I. (2008) Crystallization of hepatocyte nuclear factor 4α (HNF4α) in complex with the HNF1α promoter element. Acta Crystallogr. F64:313-7.
27. Lu, P., Rha, G. B. and Chi, Y.-I. (2007) Structural basis of disease-causing mutations in hepatocyte nuclear factor 1beta. Biochemistry 46:12071-80.
28. Lu, P., Li, Y., Gorman, A. and Chi, Y.-I. (2006) Crystallization of hepatocyte nuclear factor 1β in complex with DNA. Acta Crystallogr. F62:525-9.
29. Dhe-Paganon, S., Werner, E. D., Masahiro, N., Hansen, L., Chi, Y.-I. and Shoelson, S. E. (2004) A phenylalanine zipper mediates APS dimerization. Nature Struct. Mol. Biol. 11:968-74.
30. Duda, K., Chi, Y.-I., and Shoelson, S. E. (2004) Structural basis for HNF-4alpha activation by ligand and coactivator binding. J. Biol. Chem. 279:23311-6.
31. Kim, K.-A., Kang, K., Chi, Y.-I., Chang, I., Lee, B.-W., Min, Y.-K., Lee, M.-K., Kim, K.-W., Shoelson, S. E. and Lee, M.-.S. (2003) Identification and functional characterization of a novel mutation of hepatocyte nuclear factor-1alpha gene in a Korean family with MODY3. Diabetologia. 46:721-7.
32. Chi, Y.-I., Frantz, J. D., Oh, B.-C., Hansen, L. and Shoelson, S. E. (2002) Diabetes mutations delineate an atypical POU domain in HNF1alpha. Molecular Cell 10: 1129-37.
33. Dhe-Paganon, S., Duda, K., Iwamoto, M., Chi, Y.-I. and Shoelson, S. E. (2002) Crystal structure of HNF4alpha ligand binding domain in complex with endogenous fatty acid ligand. J. Biol. Chem. 277:37973-6.
34. Chi, Y.-I., Sadler, I., Jablonski, L. M., Callatine, S. D., Deobald, C. F., Stauffacher, C. V. and Bohach, G.A. (2002) Zinc-mediated dimerization and its effect on activity and stability of staphylococcal enterotoxin type C. J. Biol. Chem. 277:22839-46.
35. Dhe-Paganon, S., Werner, E. D., Chi, Y.-I. and Shoelson, S. E. (2002) Structure of the globular tail of nuclear lamin. J. Biol. Chem. 277:17381-4.
36. Dhe-Paganon, S., Shigeta, R., Chi, Y.-I., Ristow, M. and Shoelson, S. E. (2000) Crystal structure of human frataxin. J. Biol. Chem. 275:30753-6.
37. Chi, Y.-I., Huang, L.-S., Zhang, Z., Fernandez-Velasco, J. G., Markin, R. and Berry, E. A. (2000) High resolution structure of a soluble domain of cytochrome f from Chlamydononas reinhardtii. Biochemistry 39:7689-701.
38. Chi, Y.-I., Martinez-Cruz, L. A., Jancarik, J., Swanson, R. V., Robertson, D. E. and Kim, S.-H. (1999) Crystal structure of the beta-glycosidase from the hyperthermophilic archeon Thermosphaera aggregans: insights into its activity and thermostability. FEBS Letters 445:375-83.
39. Jang, S.-B., Hung, L. W., Chi, Y.-I., Holbrook, E. L., Carter, R. J. and Holbrook, S. R. (1998) Structure of an RNA internal loop consisting of tandem C-A base pairs. Biochemistry 37:11726-31.
40. Zhang, Z., Huang, L.-S., Schulmeister, V. M., Chi, Y.-I., Kim, K.-K., Hung, L.-W., Crofts, A. R., Berry, E. A. and Kim, S.-H. (1998) Electron transfer by domain movement in cytochrome bc1. Nature 392:677-84.
41. Chi, Y.-I., Yokota, H. and Kim, S.-H. (1997) Apo structure of the Asp receptor ligand-binding domain from Escherichia coli and its structural comparison with ligand or pseudoligand-bound forms. FEBS Letters 414:327-32.
42. Bohach, G. A., Stauffacher, C. V., Ohlendorf, D. H., Chi, Y.-I., Vath, G. M. and Schlievert, P. M. (1996) The staphylococcal and streptococcal pyrogenic toxin family. Adv. Exp. Med. Biol. 391:131-54.
43. Lawrence, C.M., Chi, Y.-I., Rodwell, V.W. and Stauffacher, C.V. (1995) Crystallization of 3-hydroxy-3-methylglutaryl coenzyme A reductase from Pseudomonas mevalonii. Acta Crystallographica D51:386-9.
44. Schlievert, P. M., Bohach, G. A., Olendorf, D. H., Stauffacher, C. V., Leung, D. Y. M., Murray, D. L., Prasad, S. P., Earhart, C. A., Jablonski, L. M., Hoffmann, M. L. and Chi, Y.-I. (1995) Molecular structure of staphylococcus and stteptococcus superantigens. J. Clinical Immun. 15:S4-10.
45. Jardetzky, T.S., Brown, J. H., Gorga, J.C., Stern, L.J., Urban, R.G., Chi, Y.-I., Stauffacher, C.V., Strominger, J.L. and Wiley, D.C. (1994) Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigen. Nature 368:711-8.
46. Hoffmann, M.L., Jablonski, L.M., Crum, K.K., Chi, Y.-I., Stauffacher, C.V., Hackett, S.P., Stevens, D.L. and Bohach, G.A. (1994) Predictions of T-cell receptor and major histocompatibility complex binding sites on staphylococcal enterotoxin C1. Infect. Immun. 62:3396-407.
47. Hovde, C.J., Marr, J.C., Hoffmann, M.L., Hackett, S.P., Chi, Y.-I., Crum, K.K., Stevens, D.L., Stauffacher, C.V. and Bohach, G.A. (1994) Investigation of the role of the disulfide bond in activity and structure of staphylococcal enterotoxin C1. Mol. Micro. 13:897-909.
48. Bohach, G.A., Chi, Y.-I. and Stauffacher, C.V. (1992) Crystallization and preliminary X-ray analysis of staphylococcal enterotoxin type C. Proteins 13:152-7.
Books, Chapters, and Reviews
1. Scott, W. G., Martick, M. and Chi, Y.-I. (2009) Structure and function of regulatory RNA elements: Ribozymes that regulate gene expression. Biochim. Biophys. Act (BBA) - Gene Regulatory Mechanism 1789:634-41.
2. Chi, Y.-I. (2005) Homeodomain revisited: A lesson from disease-causing mutations. Human Genetics 116:433-44.
3. Bohach, G. A., Jablonski, L. M., Deobald, C. F., Chi, Y.-I. and Stauffacher, C. V. (1995) Functional domains of staphylococcal enterotoxins. In Molecular Approaches to Food Safety: Issues involving Toxic Microorganisms. Alakan, Inc., Fort Collins, CO. PP. 339-56.