Qizhen Shi MD, PhD
Professor
Institution: Medical College of Wisconsin
Department: Pediatrics
Division: Hematology and Oncology - Pediatrics
Program: Oncology
Member of the Center for Immunology
I am willing to mentor a junior faculty member.
I am willing to collaborate on research projects.
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Research Experience
Hemophilia A and hemophilia B, platelet-specific gene therapy, immune responses in hemophilia A gene therapy, the association between VWF and FVIII.Publications (52)
Thromboelastometry assessment of hemostatic properties in various murine models with coagulopathy and the effect of factor VIII therapeutics. (Schroeder JA, Kuether EA, Fang J, Jing W, Weiler H, Wilcox DA, Montgomery RR, Shi Q) J Thromb Haemost 2021 Oct;19(10):2417-2427 PMID: 34245090 SCOPUS ID: 2-s2.0-85111140299 07/11/2021 Comparative Effectiveness of Aspirin Dosing in Cardiovascular Disease. (Jones WS, Mulder H, Wruck LM, Pencina MJ, Kripalani S, Muñoz D, Crenshaw DL, Effron MB, Re RN, Gupta K, Anderson RD, Pepine CJ, Handberg EM, Manning BR, Jain SK, Girotra S, Riley D, DeWalt DA, Whittle J, Goldberg YH, Roger VL, Hess R, Benziger CP, Farrehi P, Zhou L, Ford DE, Haynes K, VanWormer JJ, Knowlton KU, Kraschnewski JL, Polonsky TS, Fintel DJ, Ahmad FS, McClay JC, Campbell JR, Bell DS, Fonarow GC, Bradley SM, Paranjape A, Roe MT, Robertson HR, Curtis LH, Sharlow AG, Berdan LG, Hammill BG, Harris DF, Qualls LG, Marquis-Gravel G, Modrow MF, Marcus GM, Carton TW, Nauman E, Waitman LR, Kho AN, Shenkman EA, McTigue KM, Kaushal R, Masoudi FA, Antman EM, Davidson DR, Edgley K, Merritt JG, Brown LS, Zemon DN, McCormick TE 3rd, Alikhaani JD, Gregoire KC, Rothman RL, Harrington RA, Hernandez AF, ADAPTABLE Team) N Engl J Med 2021 05 27;384(21):1981-1990 PMID: 33999548 SCOPUS ID: 2-s2.0-85106488259 05/18/2021 10 CitationsPlatelet gene therapy induces robust immune tolerance even in a primed model via peripheral clonal deletion of antigen-specific T cells (Li J, Chen J, Schroeder JA, Hu J, Williams CB, Shi Q) Molecular Therapy - Nucleic Acids 5 March 2021;23:719-730 SCOPUS ID: 2-s2.0-85099917698 03/05/2021 Platelet-targeted hyperfunctional FIX gene therapy for hemophilia B mice even with preexisting anti-FIX immunity. (Schroeder JA, Chen J, Chen Y, Cai Y, Yu H, Mattson JG, Monahan PE, Shi Q) Blood Adv 2021 03 09;5(5):1224-1238 PMID: 33646304 PMCID: PMC7948262 SCOPUS ID: 2-s2.0-85102965352 03/02/2021 Platelet gene therapy induces robust immune tolerance even in a primed model via peripheral clonal deletion of antigen-specific T cells. (Li J, Chen J, Schroeder JA, Hu J, Williams CB, Shi Q) Mol Ther Nucleic Acids 2021 Mar 05;23:719-730 PMID: 33575117 PMCID: PMC7851450 SCOPUS ID: 2-s2.0-85099917698 02/13/2021 Platelet-Targeted FVIII Gene Therapy Restores Hemostasis and Induces Immune Tolerance for Hemophilia A. (Cai Y, Shi Q) Front Immunol 2020;11:964 PMID: 32595633 PMCID: PMC7303294 SCOPUS ID: 2-s2.0-85087185344 07/01/2020 In vivo enrichment of genetically manipulated platelets for murine hemophilia B gene therapy. (Chen Y, Schroeder JA, Gao C, Li J, Hu J, Shi Q) J Cell Physiol 2021 01;236(1):354-365 PMID: 32510630 PMCID: PMC7722216 SCOPUS ID: 2-s2.0-85086088416 06/09/2020 Unexpected enhancement of FVIII immunogenicity by endothelial expression in lentivirus-transduced and transgenic mice. (Shi Q, Carman CV, Chen Y, Sage PT, Xue F, Liang XM, Gilbert GE) Blood Adv 2020 05 26;4(10):2272-2285 PMID: 32453842 PMCID: PMC7252558 SCOPUS ID: 2-s2.0-85086840336 05/27/2020 A rat model of severe VWD by elimination of the VWF gene using CRISPR/Cas9. (Garcia J, Flood VH, Haberichter SL, Fahs SA, Mattson JG, Geurts AM, Zogg M, Weiler H, Shi Q, Montgomery RR) Res Pract Thromb Haemost 2020 Jan;4(1):64-71 PMID: 31989086 PMCID: PMC6971331 SCOPUS ID: 2-s2.0-85105291983 01/29/2020 1 CitationThe severe spontaneous bleeding phenotype in a novel hemophilia A rat model is rescued by platelet FVIII expression. (Shi Q, Mattson JG, Fahs SA, Geurts AM, Weiler H, Montgomery RR) Blood Adv 2020 01 14;4(1):55-65 PMID: 31899798 PMCID: PMC6960468 SCOPUS ID: 2-s2.0-85078174834 01/04/2020 6 CitationsInduction of activated T follicular helper cells is critical for anti-FVIII inhibitor development in hemophilia A mice. (Jing W, Chen J, Cai Y, Chen Y, Schroeder JA, Johnson BD, Cui W, Shi Q) Blood Adv 2019 10 22;3(20):3099-3110 PMID: 31648333 PMCID: PMC6849959 SCOPUS ID: 2-s2.0-85074935557 10/28/2019 9 CitationsSummary of the Third Annual Blood and Marrow Transplant Clinical Trials Network Myeloma Intergroup Workshop on Minimal Residual Disease and Immune Profiling. (Holstein SA, Al-Kadhimi Z, Costa LJ, Hahn T, Hari P, Hillengass J, Jacob A, Munshi NC, Oliva S, Pasquini MC, Shi Q, Stadtmauer EA, Waldvogel SL, McCarthy PL) Biol Blood Marrow Transplant 2020 01;26(1):e7-e15 PMID: 31526843 PMCID: PMC6942175 SCOPUS ID: 2-s2.0-85073734108 09/19/2019 6 CitationsNongenotoxic antibody-drug conjugate conditioning enables safe and effective platelet gene therapy of hemophilia A mice. (Gao C, Schroeder JA, Xue F, Jing W, Cai Y, Scheck A, Subramaniam S, Rao S, Weiler H, Czechowicz A, Shi Q) Blood Adv 2019 09 24;3(18):2700-2711 PMID: 31515232 PMCID: PMC6759737 SCOPUS ID: 2-s2.0-85072272107 09/14/2019 19 CitationsIn vivo generated hematopoietic stem cells from genome edited induced pluripotent stem cells are functional in platelet-targeted gene therapy of murine hemophilia A. (Wang D, Zhang G, Gu J, Shao X, Dai Y, Li J, Pan X, Yao S, Xu A, Jin Y, Huang J, Shi Q, Zhu J, Xi X, Chen Z, Chen S) Haematologica 2020 04;105(4):e175-e179 PMID: 31296582 PMCID: PMC7109733 SCOPUS ID: 2-s2.0-85083225461 07/13/2019 7 CitationsThe impact of GPIbα on platelet-targeted FVIII gene therapy in hemophilia A mice with pre-existing anti-FVIII immunity. (Chen J, Schroeder JA, Luo X, Montgomery RR, Shi Q) J Thromb Haemost 2019 03;17(3):449-459 PMID: 30609275 PMCID: PMC6397061 SCOPUS ID: 2-s2.0-85061041842 01/05/2019 7 CitationsPlatelet Gene Therapy Promotes Targeted Peripheral Tolerance by Clonal Deletion and Induction of Antigen-Specific Regulatory T Cells. (Luo X, Chen J, Schroeder JA, Allen KP, Baumgartner CK, Malarkannan S, Hu J, Williams CB, Shi Q) Front Immunol 2018;9:1950 PMID: 30237796 PMCID: PMC6136275 SCOPUS ID: 2-s2.0-85053056830 09/22/2018 8 CitationsPlatelet-Targeted Gene Therapy for Hemophilia. (Shi Q) Mol Ther Methods Clin Dev 2018 Jun 15;9:100-108 PMID: 29520365 PMCID: PMC5842292 SCOPUS ID: 2-s2.0-85044867723 03/10/2018 20 CitationsThe impact of von Willebrand factor on factor VIII memory immune responses. (Chen J, Schroeder JA, Luo X, Shi Q) Blood Adv 2017 Aug 22;1(19):1565-1574 PMID: 28920105 PMCID: PMC5600162 SCOPUS ID: 2-s2.0-85050807508 09/19/2017 6 CitationsImmune tolerance induced by platelet-targeted factor VIII gene therapy in hemophilia A mice is CD4 T cell mediated. (Chen Y, Luo X, Schroeder JA, Chen J, Baumgartner CK, Hu J, Shi Q) J Thromb Haemost 2017 10;15(10):1994-2004 PMID: 28799202 PMCID: PMC5630523 SCOPUS ID: 2-s2.0-85029226218 08/12/2017 18 CitationsGene therapy (Shi Q, Montgomery RR) Platelets in Thrombotic and Non-Thrombotic Disorders: Pathophysiology, Pharmacology and Therapeutics: an Update 8 March 2017:1431-1445 SCOPUS ID: 2-s2.0-85055916604 03/08/2017 TGF-β1 along with other platelet contents augments Treg cells to suppress anti-FVIII immune responses in hemophilia A mice. (Haribhai D, Luo X, Chen J, Jia S, Shi L, Schroeder JA, Weiler H, Aster RH, Hessner MJ, Hu J, Williams CB, Shi Q) Blood Adv 2016 Dec 13;1(2):139-151 PMID: 28164173 PMCID: PMC5288643 02/07/2017 Higher Fluid Balance Increases the Risk of Death From Sepsis: Results From a Large International Audit. (Sakr Y, Rubatto Birri PN, Kotfis K, Nanchal R, Shah B, Kluge S, Schroeder ME, Marshall JC, Vincent JL, Intensive Care Over Nations Investigators) Crit Care Med 2017 Mar;45(3):386-394 PMID: 27922878 SCOPUS ID: 2-s2.0-85002152455 12/07/2016 131 CitationsTargeting factor VIII expression to platelets for hemophilia A gene therapy does not induce an apparent thrombotic risk in mice. (Baumgartner CK, Mattson JG, Weiler H, Shi Q, Montgomery RR) J Thromb Haemost 2017 01;15(1):98-109 PMID: 27496751 PMCID: PMC5280575 SCOPUS ID: 2-s2.0-84997796223 08/09/2016 14 CitationsThe immunogenicity of platelet-derived FVIII in hemophilia A mice with or without preexisting anti-FVIII immunity. (Chen Y, Schroeder JA, Chen J, Luo X, Baumgartner CK, Montgomery RR, Hu J, Shi Q) Blood 2016 Mar 10;127(10):1346-54 PMID: 26668132 PMCID: PMC4786841 SCOPUS ID: 2-s2.0-84960904100 12/17/2015 14 CitationsComparison of platelet-derived and plasma factor VIII efficacy using a novel native whole blood thrombin generation assay. (Baumgartner CK, Zhang G, Kuether EL, Weiler H, Shi Q, Montgomery RR) J Thromb Haemost 2015 Dec;13(12):2210-9 PMID: 26453193 PMCID: PMC4715732 SCOPUS ID: 2-s2.0-84958773657 10/11/2015 8 CitationsThe important role of von Willebrand factor in platelet-derived FVIII gene therapy for murine hemophilia A in the presence of inhibitory antibodies. (Shi Q, Schroeder JA, Kuether EL, Montgomery RR) J Thromb Haemost 2015 Jul;13(7):1301-9 PMID: 25955153 PMCID: PMC4496307 SCOPUS ID: 2-s2.0-84935446637 05/09/2015 16 CitationsNon-myeloablative conditioning with busulfan before hematopoietic stem cell transplantation leads to phenotypic correction of murine Bernard-Soulier syndrome. (Kanaji S, Fahs SA, Ware J, Montgomery RR, Shi Q) J Thromb Haemost 2014 Oct;12(10):1726-32 PMID: 25066812 PMCID: PMC4194147 SCOPUS ID: 2-s2.0-84907878326 07/30/2014 6 CitationsNovel investigations on the protective role of the FVIII/VWF complex in inhibitor development. (Mannucci PM, Shi Q, Bonanad S, Klamroth R) Haemophilia 2014 Sep;20 Suppl 6:2-16 PMID: 24975700 SCOPUS ID: 2-s2.0-84903212652 07/01/2014 11 CitationsIn vivo enrichment of genetically manipulated platelets corrects the murine hemophilic phenotype and induces immune tolerance even using a low multiplicity of infection. (Schroeder JA, Chen Y, Fang J, Wilcox DA, Shi Q) J Thromb Haemost 2014 Aug;12(8):1283-93 PMID: 24931217 PMCID: PMC4127102 SCOPUS ID: 2-s2.0-84905667071 06/17/2014 27 CitationsA conditional knockout mouse model reveals endothelial cells as the principal and possibly exclusive source of plasma factor VIII. (Fahs SA, Hille MT, Shi Q, Weiler H, Montgomery RR) Blood 2014 Jun 12;123(24):3706-13 PMID: 24705491 PMCID: PMC4055921 SCOPUS ID: 2-s2.0-84902594994 04/08/2014 98 CitationsPlatelet Gene Therapy by Lentiviral Gene Delivery to Hematopoietic Stem Cells Restores Hemostasis and Induces Humoral Immune Tolerance in FIX(null) Mice. (Chen Y, Schroeder JA, Kuether EL, Zhang G, Shi Q) Mol Ther 2014 Jan;22(1):169-177 PMID: 28141990 01/01/2014 Platelet gene therapy corrects the hemophilic phenotype in immunocompromised hemophilia A mice transplanted with genetically manipulated human cord blood stem cells. (Shi Q, Kuether EL, Chen Y, Schroeder JA, Fahs SA, Montgomery RR) Blood 2014 Jan 16;123(3):395-403 PMID: 24269957 PMCID: PMC3894495 SCOPUS ID: 2-s2.0-84897018747 11/26/2013 34 CitationsPlatelet-targeted gene therapy with human factor VIII establishes haemostasis in dogs with haemophilia A. (Du LM, Nurden P, Nurden AT, Nichols TC, Bellinger DA, Jensen ES, Haberichter SL, Merricks E, Raymer RA, Fang J, Koukouritaki SB, Jacobi PM, Hawkins TB, Cornetta K, Shi Q, Wilcox DA) Nat Commun 2013;4:2773 PMID: 24253479 PMCID: PMC3868233 SCOPUS ID: 2-s2.0-84893419411 11/21/2013 75 CitationsPlatelet gene therapy by lentiviral gene delivery to hematopoietic stem cells restores hemostasis and induces humoral immune tolerance in FIX(null) mice. (Chen Y, Schroeder JA, Kuether EL, Zhang G, Shi Q) Mol Ther 2014 Jan;22(1):169-77 PMID: 24042561 PMCID: PMC3978792 SCOPUS ID: 2-s2.0-84891836194 09/18/2013 41 CitationsFactor VIII inhibitors: von Willebrand factor makes a difference in vitro and in vivo. (Shi Q, Kuether EL, Schroeder JA, Perry CL, Fahs SA, Cox Gill J, Montgomery RR) J Thromb Haemost 2012 Nov;10(11):2328-37 PMID: 22908929 PMCID: PMC3670966 SCOPUS ID: 2-s2.0-84868144084 08/23/2012 14 CitationsContribution of platelet vs. endothelial VWF to platelet adhesion and hemostasis. (Kanaji S, Fahs SA, Shi Q, Haberichter SL, Montgomery RR) J Thromb Haemost 2012 Aug;10(8):1646-52 PMID: 22642380 PMCID: PMC3419786 SCOPUS ID: 2-s2.0-84865237107 05/31/2012 78 CitationsLentivirus-mediated platelet gene therapy of murine hemophilia A with pre-existing anti-factor VIII immunity. (Kuether EL, Schroeder JA, Fahs SA, Cooley BC, Chen Y, Montgomery RR, Wilcox DA, Shi Q) J Thromb Haemost 2012 Aug;10(8):1570-80 PMID: 22632092 PMCID: PMC3419807 SCOPUS ID: 2-s2.0-84864333652 05/29/2012 81 CitationsPlatelet and endothelial expression of clotting factors for the treatment of hemophilia. (Montgomery RR, Shi Q) Thromb Res 2012 May;129 Suppl 2:S46-8 PMID: 22421106 PMCID: PMC3581347 SCOPUS ID: 2-s2.0-84859802515 03/17/2012 12 CitationsIntravascular recovery of VWF and FVIII following intraperitoneal injection and differences from intravenous and subcutaneous injection in mice. (Shi Q, Kuether EL, Schroeder JA, Fahs SA, Montgomery RR) Haemophilia 2012 Jul;18(4):639-46 PMID: 22221819 PMCID: PMC3323668 SCOPUS ID: 2-s2.0-84863193826 01/10/2012 14 CitationsCorrection of murine Bernard-Soulier syndrome by lentivirus-mediated gene therapy. (Kanaji S, Kuether EL, Fahs SA, Schroeder JA, Ware J, Montgomery RR, Shi Q) Mol Ther 2012 Mar;20(3):625-32 PMID: 22044935 PMCID: PMC3293608 SCOPUS ID: 2-s2.0-84857781525 11/03/2011 26 CitationsAlternative strategies for gene therapy of hemophilia. (Montgomery RR, Shi Q) Hematology Am Soc Hematol Educ Program 2010;2010:197-202 PMID: 21239794 PMCID: PMC3383974 SCOPUS ID: 2-s2.0-79955004711 01/18/2011 5 CitationsPlatelets as delivery systems for disease treatments. (Shi Q, Montgomery RR) Adv Drug Deliv Rev 2010 Sep 30;62(12):1196-203 PMID: 20619307 PMCID: PMC2991519 SCOPUS ID: 2-s2.0-78649321428 07/14/2010 30 CitationsTargeting FVIII expression to endothelial cells regenerates a releasable pool of FVIII and restores hemostasis in a mouse model of hemophilia A. (Shi Q, Fahs SA, Kuether EL, Cooley BC, Weiler H, Montgomery RR) Blood 2010 Oct 21;116(16):3049-57 PMID: 20606161 PMCID: PMC2974610 SCOPUS ID: 2-s2.0-77958193310 07/08/2010 37 CitationsFactor IX ectopically expressed in platelets can be stored in alpha-granules and corrects the phenotype of hemophilia B mice. (Zhang G, Shi Q, Fahs SA, Kuether EL, Walsh CE, Montgomery RR) Blood 2010 Aug 26;116(8):1235-43 PMID: 20445020 PMCID: PMC2938234 SCOPUS ID: 2-s2.0-77955921912 05/07/2010 37 CitationsSyngeneic transplantation of hematopoietic stem cells that are genetically modified to express factor VIII in platelets restores hemostasis to hemophilia A mice with preexisting FVIII immunity. (Shi Q, Fahs SA, Wilcox DA, Kuether EL, Morateck PA, Mareno N, Weiler H, Montgomery RR) Blood 2008 Oct 01;112(7):2713-21 PMID: 18495954 PMCID: PMC2556608 SCOPUS ID: 2-s2.0-53449090226 05/23/2008 74 CitationsLentivirus-mediated platelet-derived factor VIII gene therapy in murine haemophilia A. (Shi Q, Wilcox DA, Fahs SA, Fang J, Johnson BD, DU LM, Desai D, Montgomery RR) J Thromb Haemost 2007 Feb;5(2):352-61 PMID: 17269937 SCOPUS ID: 2-s2.0-33846453333 02/03/2007 102 CitationsFactor VIII ectopically targeted to platelets is therapeutic in hemophilia A with high-titer inhibitory antibodies. (Shi Q, Wilcox DA, Fahs SA, Weiler H, Wells CW, Cooley BC, Desai D, Morateck PA, Gorski J, Montgomery RR) J Clin Invest 2006 Jul;116(7):1974-82 PMID: 16823491 PMCID: PMC1483176 SCOPUS ID: 2-s2.0-33745864802 07/11/2006 133 CitationsRegulated release of VWF and FVIII and the biologic implications. (Haberichter SL, Shi Q, Montgomery RR) Pediatr Blood Cancer 2006 May 01;46(5):547-53 PMID: 16470522 SCOPUS ID: 2-s2.0-33645304827 02/14/2006 42 CitationsTargeting platelet GPIbalpha transgene expression to human megakaryocytes and forming a complete complex with endogenous GPIbbeta and GPIX. (Shi Q, Wilcox DA, Morateck PA, Fahs SA, Kenny D, Montgomery RR) J Thromb Haemost 2004 Nov;2(11):1989-97 PMID: 15550031 SCOPUS ID: 2-s2.0-13244262627 11/20/2004 31 CitationsInduction of megakaryocytes to synthesize and store a releasable pool of human factor VIII. (Wilcox DA, Shi Q, Nurden P, Haberichter SL, Rosenberg JB, Johnson BD, Nurden AT, White GC 2nd, Montgomery RR) J Thromb Haemost 2003 Dec;1(12):2477-89 PMID: 14675082 SCOPUS ID: 2-s2.0-1542544992 12/17/2003 47 CitationsFactor VIII ectopically expressed in platelets: efficacy in hemophilia A treatment. (Yarovoi HV, Kufrin D, Eslin DE, Thornton MA, Haberichter SL, Shi Q, Zhu H, Camire R, Fakharzadeh SS, Kowalska MA, Wilcox DA, Sachais BS, Montgomery RR, Poncz M) Blood 2003 Dec 01;102(12):4006-13 PMID: 12881300 SCOPUS ID: 2-s2.0-10744231807 07/26/2003 120 CitationsExpression of human factor VIII under control of the platelet-specific alphaIIb promoter in megakaryocytic cell line as well as storage together with VWF. (Shi Q, Wilcox DA, Fahs SA, Kroner PA, Montgomery RR) Mol Genet Metab 2003 May;79(1):25-33 PMID: 12765843 SCOPUS ID: 2-s2.0-0038522536 05/27/2003 37 Citations
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