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Thomas C. Zahrt PhD

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Professor

Institution: Medical College of Wisconsin
Department: Microbiology and Immunology


Research Experience

  • Adaptation, Physiological
  • Gene Expression Regulation, Bacterial
  • Host-Pathogen Interactions
  • Latent Tuberculosis
  • Lipid Droplet Associated Proteins
  • Lipid Droplets
  • Mycobacterium tuberculosis
  • Signal Transduction
  • Virulence
  • Methodologies and Techniques

  • Blotting, Western
  • Cloning, Molecular
  • Disease Models, Animal
  • Electrophoresis, Polyacrylamide Gel
  • Electrophoretic Mobility Shift Assay
  • Genetic Complementation Test
  • Genetic Engineering
  • Mutagenesis, Insertional
  • Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Survival Analysis
  • Leadership Positions

  • Director, Interdisciplinary Program in Biomedical Sciences
  • Member, Center for Infectious Disease Research
  • Director, Microbiology and Immunology Graduate Program
  • Educational Expertise

  • Latent Tuberculosis
  • Lipid Droplets
  • Mycobacterium tuberculosis
  • Stress, Physiological
  • Publications (34)

  • Association of Mycobacterium Proteins with Lipid Droplets. (Armstrong RM, Carter DC, Atkinson SN, Terhune SS, Zahrt TC) J Bacteriol 2018 08 15;200(16) PMID: 29760207 PMCID: PMC6060360 SCOPUS ID: 2-s2.0-85050494395 05/16/2018       3 Citations
  • Rv2744c Is a PspA Ortholog That Regulates Lipid Droplet Homeostasis and Nonreplicating Persistence in Mycobacterium tuberculosis. (Armstrong RM, Adams KL, Zilisch JE, Bretl DJ, Sato H, Anderson DM, Zahrt TC) J Bacteriol 2016 06 01;198(11):1645-1661 PMID: 27002134 PMCID: PMC4959298 03/24/2016       17 Citations
  • The MprB extracytoplasmic domain negatively regulates activation of the Mycobacterium tuberculosis MprAB two-component system. (Bretl DJ, Bigley TM, Terhune SS, Zahrt TC) J Bacteriol 2014 Jan;196(2):391-406 PMID: 24187094 PMCID: PMC3911260 11/05/2013    
  • Mechanisms of Francisella tularensis intracellular pathogenesis. (Celli J, Zahrt TC) Cold Spring Harb Perspect Med 2013 Apr 01;3(4):a010314 PMID: 23545572 PMCID: PMC3683997 SCOPUS ID: 2-s2.0-84879000118 04/03/2013       75 Citations
  • MprA and DosR coregulate a Mycobacterium tuberculosis virulence operon encoding Rv1813c and Rv1812c. (Bretl DJ, He H, Demetriadou C, White MJ, Penoske RM, Salzman NH, Zahrt TC) Infect Immun 2012 Sep;80(9):3018-33 PMID: 22689819 PMCID: PMC3418728 SCOPUS ID: 2-s2.0-84867585909 06/13/2012       26 Citations
  • Adaptation to environmental stimuli within the host: two-component signal transduction systems of Mycobacterium tuberculosis. (Bretl DJ, Demetriadou C, Zahrt TC) Microbiol Mol Biol Rev 2011 Dec;75(4):566-82 PMID: 22126994 PMCID: PMC3232741 SCOPUS ID: 2-s2.0-84455173453 12/01/2011       86 Citations
  • Components of the Rv0081-Rv0088 locus, which encodes a predicted formate hydrogenlyase complex, are coregulated by Rv0081, MprA, and DosR in Mycobacterium tuberculosis. (He H, Bretl DJ, Penoske RM, Anderson DM, Zahrt TC) J Bacteriol 2011 Oct;193(19):5105-18 PMID: 21821774 PMCID: PMC3187382 SCOPUS ID: 2-s2.0-80053603928 08/09/2011       40 Citations
  • The HtrA-like serine protease PepD interacts with and modulates the Mycobacterium tuberculosis 35-kDa antigen outer envelope protein. (White MJ, Savaryn JP, Bretl DJ, He H, Penoske RM, Terhune SS, Zahrt TC) PLoS One 2011 Mar 22;6(3):e18175 PMID: 21445360 PMCID: PMC3062566 SCOPUS ID: 2-s2.0-79952927866 03/30/2011       35 Citations
  • Identification, characterization and immunogenicity of an O-antigen capsular polysaccharide of Francisella tularensis. (Apicella MA, Post DM, Fowler AC, Jones BD, Rasmussen JA, Hunt JR, Imagawa S, Choudhury B, Inzana TJ, Maier TM, Frank DW, Zahrt TC, Chaloner K, Jennings MP, McLendon MK, Gibson BW) PLoS One 2010 Jul 06;5(7):e11060 PMID: 20625403 PMCID: PMC2897883 SCOPUS ID: 2-s2.0-77955411445 07/14/2010       61 Citations
  • PepD participates in the mycobacterial stress response mediated through MprAB and SigE. (White MJ, He H, Penoske RM, Twining SS, Zahrt TC) J Bacteriol 2010 Mar;192(6):1498-510 PMID: 20061478 PMCID: PMC2832534 SCOPUS ID: 2-s2.0-77949371118 01/12/2010       41 Citations
  • Working toward the future: insights into Francisella tularensis pathogenesis and vaccine development. (Pechous RD, McCarthy TR, Zahrt TC) Microbiol Mol Biol Rev 2009 Dec;73(4):684-711 PMID: 19946137 PMCID: PMC2786580 SCOPUS ID: 2-s2.0-71449102798 12/01/2009       79 Citations
  • A Francisella tularensis Schu S4 purine auxotroph is highly attenuated in mice but offers limited protection against homologous intranasal challenge. (Pechous RD, McCarthy TR, Mohapatra NP, Soni S, Penoske RM, Salzman NH, Frank DW, Gunn JS, Zahrt TC) PLoS One 2008 Jun 25;3(6):e2487 PMID: 18575611 PMCID: PMC2429968 SCOPUS ID: 2-s2.0-49649100202 06/26/2008       59 Citations
  • Attenuation and protective efficacy of an O-antigen-deficient mutant of Francisella tularensis LVS. (Li J, Ryder C, Mandal M, Ahmed F, Azadi P, Snyder DS, Pechous RD, Zahrt T, Inzana TJ) Microbiology (Reading) 2007 Sep;153(Pt 9):3141-3153 PMID: 17768257 SCOPUS ID: 2-s2.0-34948902287 09/05/2007       52 Citations
  • Identification of Francisella tularensis Himar1-based transposon mutants defective for replication in macrophages. (Maier TM, Casey MS, Becker RH, Dorsey CW, Glass EM, Maltsev N, Zahrt TC, Frank DW) Infect Immun 2007 Nov;75(11):5376-89 PMID: 17682043 PMCID: PMC2168294 SCOPUS ID: 2-s2.0-35648989527 08/08/2007       70 Citations
  • Genetics and genetic manipulation in Francisella tularensis. (Frank DW, Zahrt TC) Ann N Y Acad Sci 2007 Jun;1105:67-97 PMID: 17395725 SCOPUS ID: 2-s2.0-34748901912 03/31/2007       21 Citations
  • Construction and characterization of an attenuated purine auxotroph in a Francisella tularensis live vaccine strain. (Pechous R, Celli J, Penoske R, Hayes SF, Frank DW, Zahrt TC) Infect Immun 2006 Aug;74(8):4452-61 PMID: 16861631 PMCID: PMC1539594 SCOPUS ID: 2-s2.0-33746596554 07/25/2006       63 Citations
  • In vivo Himar1-based transposon mutagenesis of Francisella tularensis. (Maier TM, Pechous R, Casey M, Zahrt TC, Frank DW) Appl Environ Microbiol 2006 Mar;72(3):1878-85 PMID: 16517634 PMCID: PMC1393221 SCOPUS ID: 2-s2.0-33644941364 03/07/2006       69 Citations
  • MprAB is a stress-responsive two-component system that directly regulates expression of sigma factors SigB and SigE in Mycobacterium tuberculosis. (He H, Hovey R, Kane J, Singh V, Zahrt TC) J Bacteriol 2006 Mar;188(6):2134-43 PMID: 16513743 PMCID: PMC1428128 SCOPUS ID: 2-s2.0-33644857465 03/04/2006       110 Citations
  • Identification and characterization of a regulatory sequence recognized by Mycobacterium tuberculosis persistence regulator MprA. (He H, Zahrt TC) J Bacteriol 2005 Jan;187(1):202-12 PMID: 15601704 PMCID: PMC538824 SCOPUS ID: 2-s2.0-11144346285 12/17/2004       62 Citations
  • Construction and characterization of a highly efficient Francisella shuttle plasmid. (Maier TM, Havig A, Casey M, Nano FE, Frank DW, Zahrt TC) Appl Environ Microbiol 2004 Dec;70(12):7511-9 PMID: 15574954 PMCID: PMC535190 SCOPUS ID: 2-s2.0-10444282165 12/03/2004       126 Citations
  • Utilization of a labeled tracking oligonucleotide for visualization and quality control of spotted 70-mer arrays. (Hessner MJ, Singh VK, Wang X, Khan S, Tschannen MR, Zahrt TC) BMC Genomics 2004 Feb 09;5(1):12 PMID: 15018646 PMCID: PMC362869 SCOPUS ID: 2-s2.0-2442669076 03/17/2004       32 Citations
  • Functional analysis of the Mycobacterium tuberculosis MprAB two-component signal transduction system. (Zahrt TC, Wozniak C, Jones D, Trevett A) Infect Immun 2003 Dec;71(12):6962-70 PMID: 14638785 PMCID: PMC308901 SCOPUS ID: 2-s2.0-0345714738 11/26/2003       61 Citations
  • Molecular mechanisms regulating persistent Mycobacterium tuberculosis infection. (Zahrt TC) Microbes Infect 2003 Feb;5(2):159-67 PMID: 12650774 SCOPUS ID: 2-s2.0-0037296604 03/26/2003       67 Citations
  • Reactive nitrogen and oxygen intermediates and bacterial defenses: unusual adaptations in Mycobacterium tuberculosis. (Zahrt TC, Deretic V) Antioxid Redox Signal 2002 Feb;4(1):141-59 PMID: 11970850 SCOPUS ID: 2-s2.0-0036200214 04/24/2002       86 Citations
  • Mycobacterium tuberculosis signal transduction system required for persistent infections. (Zahrt TC, Deretic V) Proc Natl Acad Sci U S A 2001 Oct 23;98(22):12706-11 PMID: 11675502 PMCID: PMC60118 SCOPUS ID: 2-s2.0-0035940419 10/25/2001       167 Citations
  • Silencing of oxidative stress response in Mycobacterium tuberculosis: expression patterns of ahpC in virulent and avirulent strains and effect of ahpC inactivation. (Springer B, Master S, Sander P, Zahrt T, McFalone M, Song J, Papavinasasundaram KG, Colston MJ, Boettger E, Deretic V) Infect Immun 2001 Oct;69(10):5967-73 PMID: 11553532 PMCID: PMC98723 SCOPUS ID: 2-s2.0-0034826633 09/13/2001       72 Citations
  • Mapping of Mycobacterium tuberculosis katG promoters and their differential expression in infected macrophages. (Master S, Zahrt TC, Song J, Deretic V) J Bacteriol 2001 Jul;183(13):4033-9 PMID: 11395468 PMCID: PMC95287 SCOPUS ID: 2-s2.0-0034976601 06/08/2001       41 Citations
  • Mycobacterial FurA is a negative regulator of catalase-peroxidase gene katG. (Zahrt TC, Song J, Siple J, Deretic V) Mol Microbiol 2001 Mar;39(5):1174-85 PMID: 11251835 SCOPUS ID: 2-s2.0-0035100597 03/17/2001       103 Citations
  • An essential two-component signal transduction system in Mycobacterium tuberculosis. (Zahrt TC, Deretic V) J Bacteriol 2000 Jul;182(13):3832-8 PMID: 10851001 PMCID: PMC94557 SCOPUS ID: 2-s2.0-0034130852 06/13/2000       171 Citations
  • Surrogate genetics: the use of bacterial hybrids as a genetic tool. (Maloy S, Zahrt T) Methods 2000 Jan;20(1):73-9 PMID: 10610806 SCOPUS ID: 2-s2.0-0034005857 12/28/1999       4 Citations
  • Effect of mutS and recD mutations on Salmonella virulence. (Zahrt TC, Buchmeier N, Maloy S) Infect Immun 1999 Nov;67(11):6168-72 PMID: 10531283 PMCID: PMC97009 SCOPUS ID: 2-s2.0-0032709097 10/26/1999       23 Citations
  • Barriers to recombination between closely related bacteria: MutS and RecBCD inhibit recombination between Salmonella typhimurium and Salmonella typhi. (Zahrt TC, Maloy S) Proc Natl Acad Sci U S A 1997 Sep 02;94(18):9786-91 PMID: 9275203 PMCID: PMC23269 SCOPUS ID: 2-s2.0-0030968564 09/02/1997       68 Citations
  • Inactivation of mismatch repair overcomes the barrier to transduction between Salmonella typhimurium and Salmonella typhi. (Zahrt TC, Mora GC, Maloy S) J Bacteriol 1994 Mar;176(5):1527-9 PMID: 8113197 PMCID: PMC205224 SCOPUS ID: 2-s2.0-0028204784 03/01/1994       49 Citations
  • Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in Escherichia coli. (Orser CS, Lange CC, Xun L, Zahrt TC, Schneider BJ) J Bacteriol 1993 Jan;175(2):411-6 PMID: 7678243 PMCID: PMC196155 SCOPUS ID: 2-s2.0-0027476196 01/01/1993       81 Citations
  • Last update: 04/24/2020
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