Medical College of Wisconsin
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Charles R. Myers PhD

Professor

Institution: Medical College of Wisconsin
Department: Pharmacology and Toxicology
Program: Pharmacology

Member of the Cancer Center
Member of the Cardiovascular Center


Research Experience

  • Acrolein
  • Chromium Compounds
  • Cytochromes b5
  • Cytochromes c
  • Environmental Exposure
  • Environmental Microbiology
  • Environmental Restoration and Remediation
  • Iron
  • Manganese Compounds
  • Metals
  • Metals, Heavy
  • Occupational Exposure
  • Peroxiredoxins
  • Reactive Oxygen Species
  • Thioredoxin Reductase 1
  • Thioredoxin Reductase 2
  • Thioredoxins
  • Toxicology
  • Methodologies and Techniques

  • Blotting, Western
  • Cell Culture Techniques
  • Cloning, Molecular
  • Cytochromes b5
  • Endoplasmic Reticulum, Smooth
  • Membrane Potential, Mitochondrial
  • Membrane Proteins
  • Molecular Biology
  • Polymerase Chain Reaction
  • Spectrophotometry
  • Educational Expertise

  • Anti-Bacterial Agents
  • Antibiotics, Antineoplastic
  • Antibiotics, Antitubercular
  • Cytochrome P-450 Enzyme System
  • Drug Resistance, Microbial
  • Pharmacogenetics
  • Pharmacology
  • Toxicology
  • Publications

  • Mitochondria-Targeted Honokiol Confers a Striking Inhibitory Effect on Lung Cancer via Inhibiting Complex I Activity (J. Pan, Y. Lee, G. Cheng, J Zielonka, Q. Zhang, M. Bajzikova, D. Xiong, S.-W. Tsaih, M. Hardy, M. Flister, C.M. Olsen, Y. Wang, O. Vang, J. Neuzil, C. R. Myers, B. Kalyanaraman, M. You.) iScience 2018 3:192-207 05/25/2018    
  • Detection of mitochondria-generated reactive oxygen species in cells using multiple probes and methods: Potentials, pitfalls, and the future. (Cheng G, Zielonka M, Dranka B, Kumar SN, Myers CR, Bennett B, Garces AM, Dias Duarte Machado LG, Thiebaut D, Ouari O, Hardy M, Zielonka J, Kalyanaraman B) J Biol Chem 2018 06 29;293(26):10363-10380 PMID: 29739855 PMCID: PMC6028982 05/10/2018       3 Citations
  • The K-Ras effector p38γ MAPK confers intrinsic resistance to tyrosine kinase inhibitors by stimulating transcription and EGFR dephosphorylation. (Yin N, Lepp A, Ji Y, Mortensen M, Hou S, Qi XM, Myers CR, Chen G) J Biol Chem 2017 09 08;292(36):15070-15079 PMID: 28739874 PMCID: PMC5592682 07/26/2017       3 Citations
  • Enhanced targeting of mitochondrial peroxide defense by the combined use of thiosemicarbazones and inhibitors of thioredoxin reductase. (Myers CR) Free Radic Biol Med 2016 Feb;91:81-92 PMID: 26686468 12/22/2015       5 Citations
  • p38γ MAPK is required for inflammation-associated colon tumorigenesis. (Yin N, Qi X, Tsai S, Lu Y, Basir Z, Oshima K, Thomas JP, Myers CR, Stoner G, Chen G) Oncogene 2016 Feb 25;35(8):1039-48 PMID: 25961922 05/12/2015       10 Citations
  • The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death. (Xu J, Eriksson SE, Cebula M, Sandalova T, Hedström E, Pader I, Cheng Q, Myers CR, Antholine WE, Nagy P, Hellman U, Selivanova G, Lindqvist Y, Arnér ES) Cell Death Dis 2015 Jan 22;6:e1616 PMID: 25611390 PMCID: PMC4669772 01/23/2015       13 Citations
  • Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of enzymatic redox centers and analysis of reactive species formation by ESR spin trapping. (Myers JM, Cheng Q, Antholine WE, Kalyanaraman B, Filipovska A, Arnér ES, Myers CR) Free Radic Biol Med 2013 Jul;60:183-94 PMID: 23485585 PMCID: PMC3654041 03/15/2013       9 Citations
  • Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of enzymatic redox centers and analysis of reactive species formation by ESR spin trapping (J. M. Myers, Q. Cheng, W. E. Antholine, B. Kalyanaraman, A. Filipovska, E. S. J. Arnér, C. R. Myers) Free Radic Biol Med DOI: 10.1016/j.freeradbiomed.2013.02.016 02/28/2013    
  • The effects of chromium(VI) on the thioredoxin system: implications for redox regulation. (Myers CR) Free Radic Biol Med 2012 May 15;52(10):2091-107 PMID: 22542445 PMCID: PMC3955998 05/01/2012       31 Citations
  • p38γ mitogen-activated protein kinase (MAPK) confers breast cancer hormone sensitivity by switching estrogen receptor (ER) signaling from classical to nonclassical pathway via stimulating ER phosphorylation and c-Jun transcription. (Qi X, Zhi H, Lepp A, Wang P, Huang J, Basir Z, Chitambar CR, Myers CR, Chen G) J Biol Chem 2012 Apr 27;287(18):14681-91 PMID: 22399296 PMCID: PMC3340246 03/09/2012       18 Citations
  • Coenzyme Q(1) as a probe for mitochondrial complex I activity in the intact perfused hyperoxia-exposed wild-type and Nqo1-null mouse lung. (Bongard RD, Myers CR, Lindemer BJ, Baumgardt S, Gonzalez FJ, Merker MP) Am J Physiol Lung Cell Mol Physiol 2012 May 01;302(9):L949-58 PMID: 22268123 PMCID: PMC3362155 01/24/2012       3 Citations
  • The effects of acrolein on the thioredoxin system: implications for redox-sensitive signaling. (Myers CR, Myers JM, Kufahl TD, Forbes R, Szadkowski A) Mol Nutr Food Res 2011 Sep;55(9):1361-74 PMID: 21812108 PMCID: PMC3517082 08/04/2011       20 Citations
  • The intracellular redox stress caused by hexavalent chromium is selective for proteins that have key roles in cell survival and thiol redox control. (Myers JM, Antholine WE, Myers CR) Toxicology 2011 Mar 15;281(1-3):37-47 PMID: 21237240 PMCID: PMC3039098 01/18/2011       25 Citations
  • The iron-chelating drug triapine causes pronounced mitochondrial thiol redox stress. (Myers JM, Antholine WE, Zielonka J, Myers CR) Toxicol Lett 2011 Mar 05;201(2):130-6 PMID: 21195754 01/05/2011       13 Citations
  • The pro-oxidant chromium(VI) inhibits mitochondrial complex I, complex II, and aconitase in the bronchial epithelium: EPR markers for Fe-S proteins. (Myers CR, Antholine WE, Myers JM) Free Radic Biol Med 2010 Dec 15;49(12):1903-15 PMID: 20883776 PMCID: PMC3005768 10/05/2010       25 Citations
  • The selenium-independent inherent pro-oxidant NADPH oxidase activity of mammalian thioredoxin reductase and its selenium-dependent direct peroxidase activities. (Cheng Q, Antholine WE, Myers JM, Kalyanaraman B, Arnér ES, Myers CR) J Biol Chem 2010 Jul 09;285(28):21708-23 PMID: 20457604 PMCID: PMC2898413 05/12/2010       33 Citations
  • The effects of hexavalent chromium on thioredoxin reductase and peroxiredoxins in human bronchial epithelial cells. (Myers JM, Myers CR) Free Radic Biol Med 2009 Nov 15;47(10):1477-85 PMID: 19703554 PMCID: PMC2767428 08/26/2009       48 Citations
  • The effects of acrolein on peroxiredoxins, thioredoxins, and thioredoxin reductase in human bronchial epithelial cells. (Myers CR, Myers JM) Toxicology 2009 Mar 04;257(1-2):95-104 PMID: 19135121 PMCID: PMC2668956 01/13/2009       37 Citations
  • Addition of DNA to Cr(VI) and cytochrome b5 containing proteoliposomes leads to generation of DNA strand breaks and Cr(III) complexes. (Borthiry GR, Antholine WE, Myers JM, Myers CR) Chem Biodivers 2008 Aug;5(8):1545-57 PMID: 18729091 PMCID: PMC2715337 08/30/2008       5 Citations
  • Hexavalent chromium causes the oxidation of thioredoxin in human bronchial epithelial cells. (Myers JM, Antholine WE, Myers CR) Toxicology 2008 Apr 18;246(2-3):222-33 PMID: 18328613 PMCID: PMC2386998 03/11/2008       46 Citations
  • Reductive activation of hexavalent chromium by human lung epithelial cells: generation of Cr(V) and Cr(V)-thiol species. (Borthiry GR, Antholine WE, Myers JM, Myers CR) J Inorg Biochem 2008 Jul;102(7):1449-62 PMID: 18279960 PMCID: PMC2497427 02/19/2008       20 Citations
  • Acrolein oxidizes the cytosolic and mitochondrial thioredoxins in human endothelial cells. (Szadkowski A, Myers CR) Toxicology 2008 Jan 14;243(1-2):164-76 PMID: 18023956 PMCID: PMC2220080 11/21/2007       19 Citations
  • Reduction of hexavalent chromium by human cytochrome b5: generation of hydroxyl radical and superoxide. (Borthiry GR, Antholine WE, Kalyanaraman B, Myers JM, Myers CR) Free Radic Biol Med 2007 Mar 15;42(6):738-55; discussion 735-7 PMID: 17320757 PMCID: PMC1993546 02/27/2007       43 Citations
  • The outer membrane cytochromes of Shewanella oneidensis MR-1 are lipoproteins. (Myers CR, Myers JM) Lett Appl Microbiol 2004;39(5):466-70 PMID: 15482439 10/16/2004       37 Citations
  • Shewanella oneidensis MR-1 restores menaquinone synthesis to a menaquinone-negative mutant. (Myers CR, Myers JM) Appl Environ Microbiol 2004 Sep;70(9):5415-25 PMID: 15345428 PMCID: PMC520873 09/04/2004       32 Citations
  • The outer membrane protein Omp35 affects the reduction of Fe(III), nitrate, and fumarate by Shewanella oneidensis MR-1. (Maier TM, Myers CR) BMC Microbiol 2004 Jun 22;4:23 PMID: 15212692 PMCID: PMC459216 06/24/2004       18 Citations
  • Vanadium(V) reduction by Shewanella oneidensis MR-1 requires menaquinone and cytochromes from the cytoplasmic and outer membranes. (Myers JM, Antholine WE, Myers CR) Appl Environ Microbiol 2004 Mar;70(3):1405-12 PMID: 15006760 PMCID: PMC368379 03/10/2004       43 Citations
  • Cell surface exposure of the outer membrane cytochromes of Shewanella oneidensis MR-1. (Myers CR, Myers JM) Lett Appl Microbiol 2003;37(3):254-8 PMID: 12904229 08/09/2003       95 Citations
  • Identification of the gene encoding the sole physiological fumarate reductase in Shewanella oneidensis MR-1. (Maier TM, Myers JM, Myers CR) J Basic Microbiol 2003;43(4):312-27 PMID: 12872312 07/23/2003       28 Citations
  • Overlapping role of the outer membrane cytochromes of Shewanella oneidensis MR-1 in the reduction of manganese(IV) oxide. (Myers JM, Myers CR) Lett Appl Microbiol 2003;37(1):21-5 PMID: 12803550 06/14/2003       43 Citations
  • MtrB is required for proper incorporation of the cytochromes OmcA and OmcB into the outer membrane of Shewanella putrefaciens MR-1. (Myers CR, Myers JM) Appl Environ Microbiol 2002 Nov;68(11):5585-94 PMID: 12406753 PMCID: PMC129928 10/31/2002       81 Citations
  • Evaluating molar CYP1A level in fish hepatic microsomes by competitive ELISA using recombinant membrane-free CYP1A standard protein. (Tom M, Myers CR, Waterman MR) Aquat Toxicol 2002 Sep 10;59(1-2):101-14 PMID: 12088637 06/29/2002       13 Citations
  • Genetic complementation of an outer membrane cytochrome omcB mutant of Shewanella putrefaciens MR-1 requires omcB plus downstream DNA. (Myers JM, Myers CR) Appl Environ Microbiol 2002 Jun;68(6):2781-93 PMID: 12039733 PMCID: PMC123961 06/01/2002       39 Citations
  • Isolation and characterization of a Shewanella putrefaciens MR-1 electron transport regulator etrA mutant: reassessment of the role of EtrA. (Maier TM, Myers CR) J Bacteriol 2001 Aug;183(16):4918-26 PMID: 11466298 PMCID: PMC99549 07/24/2001       42 Citations
  • Cytochrome b(5) plays a key role in human microsomal chromium(VI) reduction. (Jannetto PJ, Antholine WE, Myers CR) Toxicology 2001 Feb 28;159(3):119-33 PMID: 11223168 02/27/2001       15 Citations
  • Role for outer membrane cytochromes OmcA and OmcB of Shewanella putrefaciens MR-1 in reduction of manganese dioxide. (Myers JM, Myers CR) Appl Environ Microbiol 2001 Jan;67(1):260-9 PMID: 11133454 PMCID: PMC92560 01/03/2001       160 Citations
  • Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR-1. (Myers CR, Carstens BP, Antholine WE, Myers JM) J Appl Microbiol 2000 Jan;88(1):98-106 PMID: 10735248 03/29/2000       191 Citations
  • Role of the tetraheme cytochrome CymA in anaerobic electron transport in cells of Shewanella putrefaciens MR-1 with normal levels of menaquinone. (Myers JM, Myers CR) J Bacteriol 2000 Jan;182(1):67-75 PMID: 10613864 PMCID: PMC94241 12/30/1999       142 Citations
  • Nitric oxide inhibits aldosterone synthesis by a guanylyl cyclase-independent effect. (Hanke CJ, Drewett JG, Myers CR, Campbell WB) Endocrinology 1998 Oct;139(10):4053-60 PMID: 9751482 09/29/1998       76 Citations
  • Isolation and sequence of omcA, a gene encoding a decaheme outer membrane cytochrome c of Shewanella putrefaciens MR-1, and detection of omcA homologs in other strains of S. putrefaciens. (Myers JM, Myers CR) Biochim Biophys Acta 1998 Aug 14;1373(1):237-51 PMID: 9733973 09/12/1998       76 Citations
  • Iron stimulates the rate of reduction of hexavalent chromium by human microsomes. (Myers CR, Myers JM) Carcinogenesis 1998 Jun;19(6):1029-38 PMID: 9667741 07/17/1998       23 Citations
  • Isolation and characterization of a transposon mutant of Shewanella putrefaciens MR-1 deficient in fumarate reductase. (Myers CR, Myers JM) Lett Appl Microbiol 1997 Sep;25(3):162-8 PMID: 9351256 11/14/1997       22 Citations
  • Outer membrane cytochromes of Shewanella putrefaciens MR-1: spectral analysis, and purification of the 83-kDa c-type cytochrome. (Myers CR, Myers JM) Biochim Biophys Acta 1997 Jun 12;1326(2):307-18 PMID: 9218561 06/12/1997       120 Citations
  • Antibodies to a synthetic peptide that react with flavin-containing monooxygenase (HLFMO3) in human hepatic microsomes. (Myers CR, Porgilsson B, Myers JM) J Pharmacol Toxicol Methods 1997 Mar;37(2):61-6 PMID: 9174980 03/01/1997       2 Citations
  • Replication of plasmids with the p15A origin in Shewanella putrefaciens MR-1. (Myers CR, Myers JM) Lett Appl Microbiol 1997 Mar;24(3):221-5 PMID: 9080705 03/01/1997       30 Citations
  • Cloning and sequence of cymA, a gene encoding a tetraheme cytochrome c required for reduction of iron(III), fumarate, and nitrate by Shewanella putrefaciens MR-1. (Myers CR, Myers JM) J Bacteriol 1997 Feb;179(4):1143-52 PMID: 9023196 PMCID: PMC178810 02/01/1997       204 Citations
  • Ferric iron reduction-linked growth yields of Shewanella putrefaciens MR-1. (Myers CR, Myers JM) J Appl Bacteriol 1994 Mar;76(3):253-8 PMID: 8157545 03/01/1994       73 Citations
  • Enzymatic reduction of chromium(VI) by human hepatic microsomes. (Pratt PF, Myers CR) Carcinogenesis 1993 Oct;14(10):2051-7 PMID: 8222053 10/01/1993       18 Citations
  • Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1. (Myers CR, Myers JM) J Bacteriol 1992 Jun;174(11):3429-38 PMID: 1592800 PMCID: PMC206023 06/01/1992       294 Citations
  • Microbial reduction of manganese and iron: new approaches to carbon cycling. (Nealson KH, Myers CR) Appl Environ Microbiol 1992 Feb;58(2):439-43 PMID: 1610166 PMCID: PMC195266 02/01/1992       226 Citations
  • Respiration-linked proton translocation coupled to anaerobic reduction of manganese(IV) and iron(III) in Shewanella putrefaciens MR-1. (Myers CR, Nealson KH) J Bacteriol 1990 Nov;172(11):6232-8 PMID: 2172208 PMCID: PMC526804 11/01/1990       199 Citations
  • Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. (Myers CR, Nealson KH) Science 1988 Jun 03;240(4857):1319-21 PMID: 17815852 06/03/1988    
  • Cell-cycle-specific fluctuation in cytoplasmic membrane composition in aerobically grown Rhodospirillum rubrum. (Myers CR, Collins ML) J Bacteriol 1987 Dec;169(12):5445-51 PMID: 3119564 PMCID: PMC213970 12/01/1987       16 Citations
  • Cell-cycle-specific oscillation in the composition of chromatophore membrane in Rhodospirillum rubrum. (Myers CR, Collins ML) J Bacteriol 1986 Jun;166(3):818-23 PMID: 3086290 PMCID: PMC215199 06/01/1986       21 Citations
  • Post-embedding direct immunogold detection of a protein antigen in insect tissue. (Myers CR, Bergtrom G, Crook SM, Boyer DR, Collins ML) J Histochem Cytochem 1986 Feb;34(2):221-6 PMID: 3511140 02/01/1986    
  • Last update: 11/01/2018
    jenkins-FCD Prod-299 9ef562391eceb2b8f95265c767fbba1ce5a52fd6