Medical College of Wisconsin
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Charles R. Myers PhD

Professor

Institution: Medical College of Wisconsin
Department: Pharmacology and Toxicology
Program: Pharmacology

Member of the Cancer Center


Research Experience

  • Acrolein
  • Chromium Compounds
  • Cytochromes b5
  • Cytochromes c
  • Environmental Exposure
  • Environmental Microbiology
  • Environmental Remediation
  • Iron
  • Manganese Compounds
  • Metals
  • Metals, Heavy
  • Occupational Exposure
  • Peroxiredoxins
  • Reactive Oxygen Species
  • Thioredoxin Reductase 1
  • Thioredoxin Reductase 2
  • Thioredoxins
  • Toxicology
  • Methodologies and Techniques

  • Blotting, Western
  • Cell Culture Techniques
  • Cloning, Molecular
  • Cytochromes b5
  • Endoplasmic Reticulum, Smooth
  • Membrane Potential, Mitochondrial
  • Membrane Proteins
  • Molecular Biology
  • Polymerase Chain Reaction
  • Spectrophotometry
  • Educational Expertise

  • Anti-Bacterial Agents
  • Antibiotics, Antifungal
  • Antibiotics, Antineoplastic
  • Antibiotics, Antitubercular
  • Cytochrome P-450 Enzyme System
  • Drug Resistance, Microbial
  • Pharmacogenetics
  • Pharmacology
  • Toxicology
  • Publications

  • p38γ MAPK is required for inflammation-associated colon tumorigenesis. (Yin N, Qi X, Tsai S, Lu Y, Basir Z, Oshima K, Thomas JP, Myers CR, Stoner G, Chen G) Oncogene 2015 May 11: PMID: 25961922
  • The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death. (Xu J, Eriksson SE, Cebula M, Sandalova T, Hedström E, Pader I, Cheng Q, Myers CR, Antholine WE, Nagy P, Hellman U, Selivanova G, Lindqvist Y, Arnér ES) Cell Death Dis 2015;6:e1616 PMID: 25611390
  • Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of enzymatic redox centers and analysis of reactive species formation by ESR spin trapping. (Myers JM, Cheng Q, Antholine WE, Kalyanaraman B, Filipovska A, Arnér ES, Myers CR) Free Radic Biol Med 2013 Jul;60:183-94 PMID: 23485585 PMCID: PMC3654041
  • Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of enzymatic redox centers and analysis of reactive species formation by ESR spin trapping (J. M. Myers, Q. Cheng, W. E. Antholine, B. Kalyanaraman, A. Filipovska, E. S. J. Arnér, C. R. Myers) Free Radic Biol Med DOI: 10.1016/j.freeradbiomed.2013.02.016
  • The effects of chromium(VI) on the thioredoxin system: implications for redox regulation. (Myers CR) Free Radic Biol Med 2012 May 15;52(10):2091-107 PMID: 22542445 PMCID: PMC3955998
  • p38γ mitogen-activated protein kinase (MAPK) confers breast cancer hormone sensitivity by switching estrogen receptor (ER) signaling from classical to nonclassical pathway via stimulating ER phosphorylation and c-Jun transcription. (Qi X, Zhi H, Lepp A, Wang P, Huang J, Basir Z, Chitambar CR, Myers CR, Chen G) J Biol Chem 2012 Apr 27;287(18):14681-91 PMID: 22399296 PMCID: PMC3340246
  • Coenzyme Q(1) as a probe for mitochondrial complex I activity in the intact perfused hyperoxia-exposed wild-type and Nqo1-null mouse lung. (Bongard RD, Myers CR, Lindemer BJ, Baumgardt S, Gonzalez FJ, Merker MP) Am J Physiol Lung Cell Mol Physiol 2012 May 1;302(9):L949-58 PMID: 22268123 PMCID: PMC3362155
  • The effects of acrolein on the thioredoxin system: implications for redox-sensitive signaling. (Myers CR, Myers JM, Kufahl TD, Forbes R, Szadkowski A) Mol Nutr Food Res 2011 Sep;55(9):1361-74 PMID: 21812108 PMCID: PMC3517082
  • The intracellular redox stress caused by hexavalent chromium is selective for proteins that have key roles in cell survival and thiol redox control. (Myers JM, Antholine WE, Myers CR) Toxicology 2011 Mar 15;281(1-3):37-47 PMID: 21237240 PMCID: PMC3039098
  • The iron-chelating drug triapine causes pronounced mitochondrial thiol redox stress. (Myers JM, Antholine WE, Zielonka J, Myers CR) Toxicol Lett 2011 Mar 5;201(2):130-6 PMID: 21195754
  • The pro-oxidant chromium(VI) inhibits mitochondrial complex I, complex II, and aconitase in the bronchial epithelium: EPR markers for Fe-S proteins. (Myers CR, Antholine WE, Myers JM) Free Radic Biol Med 2010 Dec 15;49(12):1903-15 PMID: 20883776 PMCID: PMC3005768
  • The selenium-independent inherent pro-oxidant NADPH oxidase activity of mammalian thioredoxin reductase and its selenium-dependent direct peroxidase activities. (Cheng Q, Antholine WE, Myers JM, Kalyanaraman B, Arnér ES, Myers CR) J Biol Chem 2010 Jul 9;285(28):21708-23 PMID: 20457604 PMCID: PMC2898413
  • The effects of hexavalent chromium on thioredoxin reductase and peroxiredoxins in human bronchial epithelial cells. (Myers JM, Myers CR) Free Radic Biol Med 2009 Nov 15;47(10):1477-85 PMID: 19703554 PMCID: PMC2767428
  • The effects of acrolein on peroxiredoxins, thioredoxins, and thioredoxin reductase in human bronchial epithelial cells. (Myers CR, Myers JM) Toxicology 2009 Mar 4;257(1-2):95-104 PMID: 19135121 PMCID: PMC2668956
  • Addition of DNA to Cr(VI) and cytochrome b5 containing proteoliposomes leads to generation of DNA strand breaks and Cr(III) complexes. (Borthiry GR, Antholine WE, Myers JM, Myers CR) Chem Biodivers 2008 Aug;5(8):1545-57 PMID: 18729091 PMCID: PMC2715337
  • Hexavalent chromium causes the oxidation of thioredoxin in human bronchial epithelial cells. (Myers JM, Antholine WE, Myers CR) Toxicology 2008 Apr 18;246(2-3):222-33 PMID: 18328613 PMCID: PMC2386998
  • Reductive activation of hexavalent chromium by human lung epithelial cells: generation of Cr(V) and Cr(V)-thiol species. (Borthiry GR, Antholine WE, Myers JM, Myers CR) J Inorg Biochem 2008 Jul;102(7):1449-62 PMID: 18279960 PMCID: PMC2497427
  • Acrolein oxidizes the cytosolic and mitochondrial thioredoxins in human endothelial cells. (Szadkowski A, Myers CR) Toxicology 2008 Jan 14;243(1-2):164-76 PMID: 18023956 PMCID: PMC2220080
  • Reduction of hexavalent chromium by human cytochrome b5: generation of hydroxyl radical and superoxide. (Borthiry GR, Antholine WE, Kalyanaraman B, Myers JM, Myers CR) Free Radic Biol Med 2007 Mar 15;42(6):738-55; discussion 735-7 PMID: 17320757 PMCID: PMC1993546
  • The outer membrane cytochromes of Shewanella oneidensis MR-1 are lipoproteins. (Myers CR, Myers JM) Lett Appl Microbiol 2004;39(5):466-70 PMID: 15482439
  • Shewanella oneidensis MR-1 restores menaquinone synthesis to a menaquinone-negative mutant. (Myers CR, Myers JM) Appl Environ Microbiol 2004 Sep;70(9):5415-25 PMID: 15345428 PMCID: PMC520873
  • The outer membrane protein Omp35 affects the reduction of Fe(III), nitrate, and fumarate by Shewanella oneidensis MR-1. (Maier TM, Myers CR) BMC Microbiol 2004 Jun 22;4:23 PMID: 15212692 PMCID: PMC459216
  • Vanadium(V) reduction by Shewanella oneidensis MR-1 requires menaquinone and cytochromes from the cytoplasmic and outer membranes. (Myers JM, Antholine WE, Myers CR) Appl Environ Microbiol 2004 Mar;70(3):1405-12 PMID: 15006760 PMCID: PMC368379
  • Cell surface exposure of the outer membrane cytochromes of Shewanella oneidensis MR-1. (Myers CR, Myers JM) Lett Appl Microbiol 2003;37(3):254-8 PMID: 12904229
  • Identification of the gene encoding the sole physiological fumarate reductase in Shewanella oneidensis MR-1. (Maier TM, Myers JM, Myers CR) J Basic Microbiol 2003;43(4):312-27 PMID: 12872312
  • Overlapping role of the outer membrane cytochromes of Shewanella oneidensis MR-1 in the reduction of manganese(IV) oxide. (Myers JM, Myers CR) Lett Appl Microbiol 2003;37(1):21-5 PMID: 12803550
  • MtrB is required for proper incorporation of the cytochromes OmcA and OmcB into the outer membrane of Shewanella putrefaciens MR-1. (Myers CR, Myers JM) Appl Environ Microbiol 2002 Nov;68(11):5585-94 PMID: 12406753 PMCID: PMC129928
  • Evaluating molar CYP1A level in fish hepatic microsomes by competitive ELISA using recombinant membrane-free CYP1A standard protein. (Tom M, Myers CR, Waterman MR) Aquat Toxicol 2002 Sep 10;59(1-2):101-14 PMID: 12088637
  • Genetic complementation of an outer membrane cytochrome omcB mutant of Shewanella putrefaciens MR-1 requires omcB plus downstream DNA. (Myers JM, Myers CR) Appl Environ Microbiol 2002 Jun;68(6):2781-93 PMID: 12039733 PMCID: PMC123961
  • Isolation and characterization of a Shewanella putrefaciens MR-1 electron transport regulator etrA mutant: reassessment of the role of EtrA. (Maier TM, Myers CR) J Bacteriol 2001 Aug;183(16):4918-26 PMID: 11466298 PMCID: PMC99549
  • Cytochrome b(5) plays a key role in human microsomal chromium(VI) reduction. (Jannetto PJ, Antholine WE, Myers CR) Toxicology 2001 Feb 28;159(3):119-33 PMID: 11223168
  • Role for outer membrane cytochromes OmcA and OmcB of Shewanella putrefaciens MR-1 in reduction of manganese dioxide. (Myers JM, Myers CR) Appl Environ Microbiol 2001 Jan;67(1):260-9 PMID: 11133454 PMCID: PMC92560
  • Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR-1. (Myers CR, Carstens BP, Antholine WE, Myers JM) J Appl Microbiol 2000 Jan;88(1):98-106 PMID: 10735248
  • Role of the tetraheme cytochrome CymA in anaerobic electron transport in cells of Shewanella putrefaciens MR-1 with normal levels of menaquinone. (Myers JM, Myers CR) J Bacteriol 2000 Jan;182(1):67-75 PMID: 10613864 PMCID: PMC94241
  • Nitric oxide inhibits aldosterone synthesis by a guanylyl cyclase-independent effect. (Hanke CJ, Drewett JG, Myers CR, Campbell WB) Endocrinology 1998 Oct;139(10):4053-60 PMID: 9751482
  • Isolation and sequence of omcA, a gene encoding a decaheme outer membrane cytochrome c of Shewanella putrefaciens MR-1, and detection of omcA homologs in other strains of S. putrefaciens. (Myers JM, Myers CR) Biochim Biophys Acta 1998 Aug 14;1373(1):237-51 PMID: 9733973
  • Iron stimulates the rate of reduction of hexavalent chromium by human microsomes. (Myers CR, Myers JM) Carcinogenesis 1998 Jun;19(6):1029-38 PMID: 9667741
  • Isolation and characterization of a transposon mutant of Shewanella putrefaciens MR-1 deficient in fumarate reductase. (Myers CR, Myers JM) Lett Appl Microbiol 1997 Sep;25(3):162-8 PMID: 9351256
  • Outer membrane cytochromes of Shewanella putrefaciens MR-1: spectral analysis, and purification of the 83-kDa c-type cytochrome. (Myers CR, Myers JM) Biochim Biophys Acta 1997 Jun 12;1326(2):307-18 PMID: 9218561
  • Antibodies to a synthetic peptide that react with flavin-containing monooxygenase (HLFMO3) in human hepatic microsomes. (Myers CR, Porgilsson B, Myers JM) J Pharmacol Toxicol Methods 1997 Mar;37(2):61-6 PMID: 9174980
  • Replication of plasmids with the p15A origin in Shewanella putrefaciens MR-1. (Myers CR, Myers JM) Lett Appl Microbiol 1997 Mar;24(3):221-5 PMID: 9080705
  • Cloning and sequence of cymA, a gene encoding a tetraheme cytochrome c required for reduction of iron(III), fumarate, and nitrate by Shewanella putrefaciens MR-1. (Myers CR, Myers JM) J Bacteriol 1997 Feb;179(4):1143-52 PMID: 9023196 PMCID: PMC178810
  • Ferric iron reduction-linked growth yields of Shewanella putrefaciens MR-1. (Myers CR, Myers JM) J Appl Bacteriol 1994 Mar;76(3):253-8 PMID: 8157545
  • Enzymatic reduction of chromium(VI) by human hepatic microsomes. (Pratt PF, Myers CR) Carcinogenesis 1993 Oct;14(10):2051-7 PMID: 8222053
  • Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1. (Myers CR, Myers JM) J Bacteriol 1992 Jun;174(11):3429-38 PMID: 1592800 PMCID: PMC206023
  • Microbial reduction of manganese and iron: new approaches to carbon cycling. (Nealson KH, Myers CR) Appl Environ Microbiol 1992 Feb;58(2):439-43 PMID: 1610166 PMCID: PMC195266
  • Respiration-linked proton translocation coupled to anaerobic reduction of manganese(IV) and iron(III) in Shewanella putrefaciens MR-1. (Myers CR, Nealson KH) J Bacteriol 1990 Nov;172(11):6232-8 PMID: 2172208 PMCID: PMC526804
  • Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. (Myers CR, Nealson KH) Science 1988 Jun 3;240(4857):1319-21 PMID: 17815852
  • Cell-cycle-specific fluctuation in cytoplasmic membrane composition in aerobically grown Rhodospirillum rubrum. (Myers CR, Collins ML) J Bacteriol 1987 Dec;169(12):5445-51 PMID: 3119564 PMCID: PMC213970
  • Cell-cycle-specific oscillation in the composition of chromatophore membrane in Rhodospirillum rubrum. (Myers CR, Collins ML) J Bacteriol 1986 Jun;166(3):818-23 PMID: 3086290 PMCID: PMC215199
  • Post-embedding direct immunogold detection of a protein antigen in insect tissue. (Myers CR, Bergtrom G, Crook SM, Boyer DR, Collins ML) J Histochem Cytochem 1986 Feb;34(2):221-6 PMID: 3511140
  • Last update: 07/27/2015