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Debebe Gebremedhin PhD

Debebe Gebremedhin PhD

Associate Professor

Institution: Medical College of Wisconsin
Department: Physiology
Program: Cardiovascular Research Center

Member of the Cardiovascular Center

Research Experience

  • Astrocytes
  • Cerebral Arteries
  • Potassium Channels
  • Methodologies and Techniques

  • Cell Culture Techniques
  • Electrophysiology
  • Patch-Clamp Techniques
  • Publications

  • Regulation of Cerebral Blood Flow: Response to Cytochrome P450 Lipid Metabolites. (Harder DR, Rarick KR, Gebremedhin D, Cohen SS) Compr Physiol 2018 03 25;8(2):801-821 PMID: 29687906 04/25/2018    
  • Detection of TRPV4 channel current-like activity in Fawn Hooded hypertensive (FHH) rat cerebral arterial muscle cells. (Gebremedhin D, Zhang DX, Weihrauch D, Uche NN, Harder DR) PLoS One 2017;12(5):e0176796 PMID: 28472069 PMCID: PMC5417564 05/05/2017    
  • Inhibition of soluble epoxide hydrolase augments astrocyte release of vascular endothelial growth factor and neuronal recovery after oxygen-glucose deprivation. (Zhang Y, Hong G, Lee KS, Hammock BD, Gebremedhin D, Harder DR, Koehler RC, Sapirstein A) J Neurochem 2017 03;140(5):814-825 PMID: 28002622 PMCID: PMC5310989 12/22/2016       10 Citations
  • Expression of CYP 4A ω-hydroxylase and formation of 20-hydroxyeicosatetreanoic acid (20-HETE) in cultured rat brain astrocytes. (Gebremedhin D, Zhang DX, Carver KA, Rau N, Rarick KR, Roman RJ, Harder DR) Prostaglandins Other Lipid Mediat 2016 07;124:16-26 PMID: 27174801 PMCID: PMC5287377 05/14/2016    
  • Contribution of epoxyeicosatrienoic acids to the cerebral blood flow response to hypoxemia. (Liu X, Gebremedhin D, Harder DR, Koehler RC) J Appl Physiol (1985) 2015 Nov 15;119(10):1202-9 PMID: 25792716 PMCID: PMC4747900 03/21/2015       9 Citations
  • Calculations for the one-dimensional soft Coulomb problem and the hard Coulomb limit. (Gebremedhin DH, Weatherford CA) Phys Rev E Stat Nonlin Soft Matter Phys 2014 May;89(5):053319 PMID: 25353926 10/30/2014    
  • Enhanced large conductance K+ channel activity contributes to the impaired myogenic response in the cerebral vasculature of Fawn Hooded Hypertensive rats. (Pabbidi MR, Mazur O, Fan F, Farley JM, Gebremedhin D, Harder DR, Roman RJ) Am J Physiol Heart Circ Physiol 2014 Apr 01;306(7):H989-H1000 PMID: 24464756 PMCID: PMC3962634 01/28/2014       14 Citations
  • Endogenous events modulating myogenic regulation of cerebrovascular function. (Gebremedhin D, Gopalakrishnan S, Harder DR) Curr Vasc Pharmacol 2014;12(6):810-7 PMID: 24066934 09/27/2013       9 Citations
  • Redox signaling via oxidative inactivation of PTEN modulates pressure-dependent myogenic tone in rat middle cerebral arteries. (Gebremedhin D, Terashvili M, Wickramasekera N, Zhang DX, Rau N, Miura H, Harder DR) PLoS One 2013;8(7):e68498 PMID: 23861911 PMCID: PMC3702596 07/19/2013       14 Citations
  • Arachidonic acid-induced dilation in human coronary arterioles: convergence of signaling mechanisms on endothelial TRPV4-mediated Ca2+ entry. (Zheng X, Zinkevich NS, Gebremedhin D, Gauthier KM, Nishijima Y, Fang J, Wilcox DA, Campbell WB, Gutterman DD, Zhang DX) J Am Heart Assoc 2013 Apr 25;2(3):e000080 PMID: 23619744 PMCID: PMC3698766 04/27/2013       40 Citations
  • Role of dual-specificity protein phosphatase-5 in modulating the myogenic response in rat cerebral arteries. (Wickramasekera NT, Gebremedhin D, Carver KA, Vakeel P, Ramchandran R, Schuett A, Harder DR) J Appl Physiol (1985) 2013 Jan 15;114(2):252-61 PMID: 23172031 PMCID: PMC3544499 11/23/2012       4 Citations
  • Protective effect of 20-HETE inhibition in a model of oxygen-glucose deprivation in hippocampal slice cultures. (Renic M, Kumar SN, Gebremedhin D, Florence MA, Gerges NZ, Falck JR, Harder DR, Roman RJ) Am J Physiol Heart Circ Physiol 2012 Mar 15;302(6):H1285-93 PMID: 22245774 PMCID: PMC3311475 01/17/2012       22 Citations
  • H2O2-induced dilation in human coronary arterioles: role of protein kinase G dimerization and large-conductance Ca2+-activated K+ channel activation. (Zhang DX, Borbouse L, Gebremedhin D, Mendoza SA, Zinkevich NS, Li R, Gutterman DD) Circ Res 2012 Feb 03;110(3):471-80 PMID: 22158710 PMCID: PMC3272100 12/14/2011       94 Citations
  • Epoxyeicosatrienoic acid-dependent cerebral vasodilation evoked by metabotropic glutamate receptor activation in vivo. (Liu X, Li C, Gebremedhin D, Hwang SH, Hammock BD, Falck JR, Roman RJ, Harder DR, Koehler RC) Am J Physiol Heart Circ Physiol 2011 Aug;301(2):H373-81 PMID: 21602473 PMCID: PMC3154671 05/24/2011       24 Citations
  • Pressure-induced myogenic tone and role of 20-HETE in mediating autoregulation of cerebral blood flow. (Harder DR, Narayanan J, Gebremedhin D) Am J Physiol Heart Circ Physiol 2011 May;300(5):H1557-65 PMID: 21257913 PMCID: PMC3283039 01/25/2011       35 Citations
  • Adenosine can mediate its actions through generation of reactive oxygen species. (Gebremedhin D, Weinberger B, Lourim D, Harder DR) J Cereb Blood Flow Metab 2010 Oct;30(10):1777-90 PMID: 20531463 PMCID: PMC3023402 06/10/2010       22 Citations
  • Onset of pulmonary ventilation in fetal sheep produces pial arteriolar constriction dependent on cytochrome p450 omega-hydroxylase activity. (Ohata H, Gebremedhin D, Narayanan J, Harder DR, Koehler RC) J Appl Physiol (1985) 2010 Aug;109(2):412-7 PMID: 20489034 PMCID: PMC2928585 05/22/2010       9 Citations
  • Antiangiogenic effect of inhibitors of cytochrome P450 on rats with glioblastoma multiforme. (Zagorac D, Jakovcevic D, Gebremedhin D, Harder DR) J Cereb Blood Flow Metab 2008 Aug;28(8):1431-9 PMID: 18414496 PMCID: PMC2637201 04/17/2008       23 Citations
  • 20-HETE increases superoxide production and activates NAPDH oxidase in pulmonary artery endothelial cells. (Medhora M, Chen Y, Gruenloh S, Harland D, Bodiga S, Zielonka J, Gebremedhin D, Gao Y, Falck JR, Anjaiah S, Jacobs ER) Am J Physiol Lung Cell Mol Physiol 2008 May;294(5):L902-11 PMID: 18296498 PMCID: PMC2586843 02/26/2008       49 Citations
  • Role of 20-HETE in the hypoxia-induced activation of Ca2+-activated K+ channel currents in rat cerebral arterial muscle cells. (Gebremedhin D, Yamaura K, Harder DR) Am J Physiol Heart Circ Physiol 2008 Jan;294(1):H107-20 PMID: 17906097 10/02/2007       39 Citations
  • Interaction of mechanisms involving epoxyeicosatrienoic acids, adenosine receptors, and metabotropic glutamate receptors in neurovascular coupling in rat whisker barrel cortex. (Shi Y, Liu X, Gebremedhin D, Falck JR, Harder DR, Koehler RC) J Cereb Blood Flow Metab 2008 Jan;28(1):111-25 PMID: 17519974 PMCID: PMC2204069 05/24/2007       53 Citations
  • Reactive oxygen species cerebral autoregulation in health and disease. (Terashvili M, Pratt PF, Gebremedhin D, Narayanan J, Harder DR) Pediatr Clin North Am 2006 Oct;53(5):1029-37, xi PMID: 17027622 PMCID: PMC2533262 10/10/2006       13 Citations
  • Contribution of epoxyeicosatrienoic acids to the hypoxia-induced activation of Ca2+ -activated K+ channel current in cultured rat hippocampal astrocytes. (Yamaura K, Gebremedhin D, Zhang C, Narayanan J, Hoefert K, Jacobs ER, Koehler RC, Harder DR) Neuroscience 2006 Dec;143(3):703-16 PMID: 17027168 10/10/2006       35 Citations
  • Role of astrocytes in cerebrovascular regulation. (Koehler RC, Gebremedhin D, Harder DR) J Appl Physiol (1985) 2006 Jan;100(1):307-17 PMID: 16357084 PMCID: PMC1819408 12/17/2005       200 Citations
  • Degenerin/epithelial Na+ channel proteins: components of a vascular mechanosensor. (Drummond HA, Gebremedhin D, Harder DR) Hypertension 2004 Nov;44(5):643-8 PMID: 15381679 09/24/2004       108 Citations
  • Effects of a 20-HETE antagonist and agonists on cerebral vascular tone. (Yu M, Cambj-Sapunar L, Kehl F, Maier KG, Takeuchi K, Miyata N, Ishimoto T, Reddy LM, Falck JR, Gebremedhin D, Harder DR, Roman RJ) Eur J Pharmacol 2004 Feb 23;486(3):297-306 PMID: 14985052 02/27/2004       53 Citations
  • Metabotropic glutamate receptor activation enhances the activities of two types of Ca2+-activated k+ channels in rat hippocampal astrocytes. (Gebremedhin D, Yamaura K, Zhang C, Bylund J, Koehler RC, Harder DR) J Neurosci 2003 Mar 01;23(5):1678-87 PMID: 12629172 03/12/2003       66 Citations
  • Astrocytes function in matching blood flow to metabolic activity. (Harder DR, Zhang C, Gebremedhin D) News Physiol Sci 2002 Feb;17:27-31 PMID: 11821533 02/01/2002       67 Citations
  • Production of 20-HETE and its role in autoregulation of cerebral blood flow. (Gebremedhin D, Lange AR, Lowry TF, Taheri MR, Birks EK, Hudetz AG, Narayanan J, Falck JR, Okamoto H, Roman RJ, Nithipatikom K, Campbell WB, Harder DR) Circ Res 2000 Jul 07;87(1):60-5 PMID: 10884373 07/08/2000       257 Citations
  • Peroxynitrite reversibly inhibits Ca(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells. (Brzezinska AK, Gebremedhin D, Chilian WM, Kalyanaraman B, Elliott SJ) Am J Physiol Heart Circ Physiol 2000 Jun;278(6):H1883-90 PMID: 10843885 06/14/2000       93 Citations
  • Molecular mechanisms controlling nutritive blood flow: role of cytochrome P450 enzymes. (Harder DR, Roman RJ, Gebremedhin D) Acta Physiol Scand 2000 Apr;168(4):543-9 PMID: 10759591 04/12/2000       33 Citations
  • Cannabinoid CB1 receptor of cat cerebral arterial muscle functions to inhibit L-type Ca2+ channel current. (Gebremedhin D, Lange AR, Campbell WB, Hillard CJ, Harder DR) Am J Physiol 1999 06;276(6):H2085-93 PMID: 10362691 06/11/1999       203 Citations
  • A common pathway for regulation of nutritive blood flow to the brain: arterial muscle membrane potential and cytochrome P450 metabolites. (Harder DR, Roman RJ, Gebremedhin D, Birks EK, Lange AR) Acta Physiol Scand 1998 Dec;164(4):527-32 PMID: 9887975 01/15/1999       27 Citations
  • Bioassay of an endothelium-derived hyperpolarizing factor from bovine coronary arteries: role of a cytochrome P450 metabolite. (Gebremedhin D, Harder DR, Pratt PF, Campbell WB) J Vasc Res 1998 Jul-Aug;35(4):274-84 PMID: 9701712 08/14/1998       85 Citations
  • Cat cerebral arterial smooth muscle cells express cytochrome P450 4A2 enzyme and produce the vasoconstrictor 20-HETE which enhances L-type Ca2+ current. (Gebremedhin D, Lange AR, Narayanan J, Aebly MR, Jacobs ER, Harder DR) J Physiol 1998 Mar 15;507 ( Pt 3):771-81 PMID: 9508838 PMCID: PMC2230829 05/23/1998       157 Citations
  • Functional hyperemia in the brain: hypothesis for astrocyte-derived vasodilator metabolites. (Harder DR, Alkayed NJ, Lange AR, Gebremedhin D, Roman RJ) Stroke 1998 Jan;29(1):229-34 PMID: 9445355 01/28/1998       171 Citations
  • 20-Hydroxyeicosatetraenoic acid-induced vasoconstriction and inhibition of potassium current in cerebral vascular smooth muscle is dependent on activation of protein kinase C. (Lange A, Gebremedhin D, Narayanan J, Harder D) J Biol Chem 1997 Oct 24;272(43):27345-52 PMID: 9341185 10/27/1997       146 Citations
  • Cytochrome P450 metabolites of arachidonic acid as intracellular signaling molecules in vascular tissue. (Harder DR, Lange AR, Gebremedhin D, Birks EK, Roman RJ) J Vasc Res 1997 May-Jun;34(3):237-43 PMID: 9226306 05/01/1997       139 Citations
  • Stereospecific effects of epoxyeicosatrienoic acids on renal vascular tone and K(+)-channel activity. (Zou AP, Fleming JT, Falck JR, Jacobs ER, Gebremedhin D, Harder DR, Roman RJ) Am J Physiol 1996 May;270(5 Pt 2):F822-32 PMID: 8928844 05/01/1996       179 Citations
  • Molecular characterization of an arachidonic acid epoxygenase in rat brain astrocytes. (Alkayed NJ, Narayanan J, Gebremedhin D, Medhora M, Roman RJ, Harder DR) Stroke 1996 May;27(5):971-9 PMID: 8623121 05/01/1996       158 Citations
  • Identification of epoxyeicosatrienoic acids as endothelium-derived hyperpolarizing factors. (Campbell WB, Gebremedhin D, Pratt PF, Harder DR) Circ Res 1996 Mar;78(3):415-23 PMID: 8593700 03/01/1996       1025 Citations
  • 20-HETE is an endogenous inhibitor of the large-conductance Ca(2+)-activated K+ channel in renal arterioles. (Zou AP, Fleming JT, Falck JR, Jacobs ER, Gebremedhin D, Harder DR, Roman RJ) Am J Physiol 1996 Jan;270(1 Pt 2):R228-37 PMID: 8769806 01/01/1996       258 Citations
  • Coexistence of two types of Ca(2+)-activated K+ channels in rat renal arterioles. (Gebremedhin D, Kaldunski M, Jacobs ER, Harder DR, Roman RJ) Am J Physiol 1996 Jan;270(1 Pt 2):F69-81 PMID: 8769824 01/01/1996       78 Citations
  • Shear activated channels in cell-attached patches of cultured bovine aortic endothelial cells. (Jacobs ER, Cheliakine C, Gebremedhin D, Birks EK, Davies PF, Harder DR) Pflugers Arch 1995 Nov;431(1):129-31 PMID: 8584410 11/01/1995       70 Citations
  • Halothane and isoflurane decrease the open state probability of K+ channels in dog cerebral arterial muscle cells. (Eskinder H, Gebremedhin D, Lee JG, Rusch NJ, Supan FD, Kampine JP, Bosnjak ZJ) Anesthesiology 1995 Feb;82(2):479-90 PMID: 7856906 02/01/1995       20 Citations
  • Transduction of physical force by the vascular wall Role of phospholipase C and cytochrome P450 metabolites of arachidonic acid. (Harder DR, Narayanan J, Gebremedhin D, Roman RJ) Trends Cardiovasc Med 1995 Jan-Feb;5(1):7-14 PMID: 21232232 01/01/1995    
  • Hypoxia increases the activity of Ca(2+)-sensitive K+ channels in cat cerebral arterial muscle cell membranes. (Gebremedhin D, Bonnet P, Greene AS, England SK, Rusch NJ, Lombard JH, Harder DR) Pflugers Arch 1994 Oct;428(5-6):621-30 PMID: 7838685 10/01/1994       91 Citations
  • Formation and action of a P-450 4A metabolite of arachidonic acid in cat cerebral microvessels. (Harder DR, Gebremedhin D, Narayanan J, Jefcoat C, Falck JR, Campbell WB, Roman R) Am J Physiol 1994 May;266(5 Pt 2):H2098-107 PMID: 8203608 05/01/1994       220 Citations
  • Elevated renovascular tone in young spontaneously hypertensive rats. Role of cytochrome P-450. (Imig JD, Falck JR, Gebremedhin D, Harder DR, Roman RJ) Hypertension 1993 Sep;22(3):357-64 PMID: 8349328 09/01/1993       103 Citations
  • Functional aspects of eicosanoid hydroxylation by lung and kidney cytochromes P450. Expression of cDNAs in mammalian cells and E. coli. (Masters BS, Clark JE, Roman LJ, Nishimoto M, McCabe TJ, Ortiz de Montellano PR, Plopper CG, Gebremedhin D, Ma YH, Harder DR) J Lipid Mediat 1993 Mar-Apr;6(1-3):353-60 PMID: 8357994 03/01/1993       11 Citations
  • 20-Hydroxyeicosatetraenoic acid is an endogenous vasoconstrictor of canine renal arcuate arteries. (Ma YH, Gebremedhin D, Schwartzman ML, Falck JR, Clark JE, Masters BS, Harder DR, Roman RJ) Circ Res 1993 Jan;72(1):126-36 PMID: 8417836 01/01/1993       239 Citations
  • Modulation of vascular tone in renal microcirculation by erythrocytes: role of EDRF. (Imig JD, Gebremedhin D, Harder DR, Roman RJ) Am J Physiol 1993 Jan;264(1 Pt 2):H190-5 PMID: 8430846 01/01/1993       34 Citations
  • Role of cytochrome P-450 in elevating renal vascular tone in spontaneously hypertensive rats. (Gebremedhin D, Ma YH, Imig JD, Harder DR, Roman RJ) J Vasc Res 1993 Jan-Feb;30(1):53-60 PMID: 8435472 01/01/1993       53 Citations
  • Mechanism of action of cerebral epoxyeicosatrienoic acids on cerebral arterial smooth muscle. (Gebremedhin D, Ma YH, Falck JR, Roman RJ, VanRollins M, Harder DR) Am J Physiol 1992 Aug;263(2 Pt 2):H519-25 PMID: 1510149 08/01/1992       262 Citations
  • Effects of hypoxia on a potassium channel in cat cerebral arterial muscle cells. (Bonnet P, Gebremedhin D, Rush NJ, Harder DR) Z Kardiol 1991;80 Suppl 7:25-7 PMID: 1792816 01/01/1991       10 Citations
  • Enhanced vascular tone in the renal vasculature of spontaneously hypertensive rats. (Gebremedhin D, Fenoy FJ, Harder DR, Roman RJ) Hypertension 1990 Dec;16(6):648-54 PMID: 2246032 12/01/1990       49 Citations
  • Altered responsiveness of diabetic dog renal arteries to acetylcholine and phenylephrine: role of endothelium. (Gebremedhin D, Koltai MZ, Pogátsa G, Magyar K, Hadházy P) Pharmacology 1989;38(3):177-84 PMID: 2727056 01/01/1989       15 Citations
  • Influence of experimental diabetes on the mechanical responses of canine coronary arteries: role of endothelium. (Gebremedhin D, Koltai MZ, Pogátsa G, Magyar K, Hadházy P) Cardiovasc Res 1988 Aug;22(8):537-44 PMID: 3150300 08/01/1988       30 Citations
  • Contractile and relaxant responses of diabetic dog femoral arteries. (Gebremedhin D, Hadházy P, Koltai MZ, Pogátsa G) Acta Physiol Hung 1988;71(2):213-7 PMID: 3389165 01/01/1988       4 Citations
  • Inhibition by quinine of endothelium-dependent relaxation of rabbit aortic strips. (Gebremedhin D, Hadházy P, Magyar K) Br J Pharmacol 1987 Dec;92(4):835-41 PMID: 2827827 PMCID: PMC1853706 12/01/1987       5 Citations
  • Differential contractile responsiveness of femoral arteries from healthy and diabetic dogs: role of endothelium. (Gebremedhin D, Koltai MZ, Pogátsa G, Magyar K, Hadházy P) Arch Int Pharmacodyn Ther 1987 Jul;288(1):100-8 PMID: 3662696 07/01/1987       15 Citations
  • Species specificity of thrombin-induced changes in vascular tone. (Gebremedhin D, Ballagi-Pordány G, Hadházy P, Magyar K, Machovich R) Eur J Pharmacol 1986 Dec 02;132(1):71-4 PMID: 3816967 12/02/1986       8 Citations
  • Last update: 04/25/2018
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