Medical College of Wisconsin
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Kenneth Matthew Scaglione PhD

Assistant Professor

Institution: Medical College of Wisconsin
Department: Biochemistry

Member of the Cancer Center
Member of the Cardiovascular Center
Member of the Neuroscience Research Center


Research Experience

  • Neurofibrillary Tangles
  • Polyubiquitin
  • Proteasome Endopeptidase Complex
  • Protein Processing, Post-Translational
  • Ubiquitin
  • Ubiquitin-Activating Enzymes
  • Ubiquitin-Conjugating Enzymes
  • Ubiquitin-Protein Ligase Complexes
  • Ubiquitin-Protein Ligases
  • Ubiquitination
  • MCW Program / Core Facilities

  • neuroscience research center
  • Publications

  • Interaction of the polyglutamine protein ataxin-3 with Rad23 regulates toxicity in Drosophila models of Spinocerebellar Ataxia Type 3. (Sutton JR, Blount JR, Libohova K, Tsou WL, Joshi GS, Paulson HL, Costa MDC, Scaglione KM, Todi SV) Hum Mol Genet 2017 Apr 15;26(8):1419-1431 PMID: 28158474 02/06/2017
  • Erratum to: The loop-less (tm)Cdc34 E2 mutant defective polyubiquitination in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2. (Lass A, Cocklin R, Scaglione KM, Skowyra M, Korolev S, Goebl M, Skowyra D) Cell Div 2016;11:13 PMID: 27761151 PMCID: PMC5054535 10/21/2016
  • USP5 Is Dispensable for Monoubiquitin Maintenance in Drosophila. (Ristic G, Tsou WL, Guzi E, Kanack AJ, Scaglione KM, Todi SV) J Biol Chem 2016 Apr 22;291(17):9161-72 PMID: 26917723 PMCID: PMC4861482 02/27/2016
  • Loss of the Ubiquitin-conjugating Enzyme UBE2W Results in Susceptibility to Early Postnatal Lethality and Defects in Skin, Immune, and Male Reproductive Systems. (Wang B, Merillat SA, Vincent M, Huber AK, Basrur V, Mangelberger D, Zeng L, Elenitoba-Johnson K, Miller RA, Irani DN, Dlugosz AA, Schnell S, Scaglione KM, Paulson HL) J Biol Chem 2016 Feb 05;291(6):3030-42 PMID: 26601958 PMCID: PMC4742764 11/26/2015
  • The Social Amoeba Dictyostelium discoideum Is Highly Resistant to Polyglutamine Aggregation. (Santarriaga S, Petersen A, Ndukwe K, Brandt A, Gerges N, Bruns Scaglione J, Scaglione KM) J Biol Chem 2015 Oct 16;290(42):25571-8 PMID: 26330554 PMCID: PMC4646202 09/04/2015
  • Allosteric regulation of deubiquitylase activity through ubiquitination. (Faggiano S, Menon RP, Kelly GP, Todi SV, Scaglione KM, Konarev PV, Svergun DI, Paulson HL, Pastore A) Front Mol Biosci 2015;2:2 PMID: 25988170 PMCID: PMC4428445 05/20/2015
  • A bipartite interaction between Hsp70 and CHIP regulates ubiquitination of chaperoned client proteins. (Zhang H, Amick J, Chakravarti R, Santarriaga S, Schlanger S, McGlone C, Dare M, Nix JC, Scaglione KM, Stuehr DJ, Misra S, Page RC) Structure 2015 Mar 03;23(3):472-82 PMID: 25684577 PMCID: PMC4351142 02/17/2015
  • Intrinsic disorder drives N-terminal ubiquitination by Ube2w. (Vittal V, Shi L, Wenzel DM, Scaglione KM, Duncan ED, Basrur V, Elenitoba-Johnson KS, Baker D, Paulson HL, Brzovic PS, Klevit RE) Nat Chem Biol 2015 Jan;11(1):83-9 PMID: 25436519 PMCID: PMC4270946 12/02/2014
  • Ubiquitin-binding site 2 of ataxin-3 prevents its proteasomal degradation by interacting with Rad23. (Blount JR, Tsou WL, Ristic G, Burr AA, Ouyang M, Galante H, Scaglione KM, Todi SV) Nat Commun 2014 Aug 21;5:4638 PMID: 25144244 PMCID: PMC4237202 08/22/2014
  • Enzymatic production of mono-ubiquitinated proteins for structural studies: The example of the Josephin domain of ataxin-3. (Faggiano S, Menon RP, Kelly GP, McCormick J, Todi SV, Scaglione KM, Paulson HL, Pastore A) FEBS Open Bio 2013;3:453-8 PMID: 24251111 PMCID: PMC3829987 11/20/2013
  • Ubiquitination regulates the neuroprotective function of the deubiquitinase ataxin-3 in vivo. (Tsou WL, Burr AA, Ouyang M, Blount JR, Scaglione KM, Todi SV) J Biol Chem 2013 Nov 29;288(48):34460-9 PMID: 24106274 PMCID: PMC3843061 10/10/2013
  • Accelerated neurodegeneration through chaperone-mediated oligomerization of tau. (Blair LJ, Nordhues BA, Hill SE, Scaglione KM, O'Leary JC 3rd, Fontaine SN, Breydo L, Zhang B, Li P, Wang L, Cotman C, Paulson HL, Muschol M, Uversky VN, Klengel T, Binder EB, Kayed R, Golde TE, Berchtold N, Dickey CA) J Clin Invest 2013 Oct;123(10):4158-69 PMID: 23999428 PMCID: PMC3784538 09/04/2013
  • The E3 ubiquitin ligase CHIP and the molecular chaperone Hsc70 form a dynamic, tethered complex. (Smith MC, Scaglione KM, Assimon VA, Patury S, Thompson AD, Dickey CA, Southworth DR, Paulson HL, Gestwicki JE, Zuiderweg ER) Biochemistry 2013 Aug 13;52(32):5354-64 PMID: 23865999 PMCID: PMC3856692 07/20/2013
  • The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates. (Scaglione KM, Basrur V, Ashraf NS, Konen JR, Elenitoba-Johnson KS, Todi SV, Paulson HL) J Biol Chem 2013 Jun 28;288(26):18784-8 PMID: 23696636 PMCID: PMC3696654 05/23/2013
  • CGG repeat-associated translation mediates neurodegeneration in fragile X tremor ataxia syndrome. (Todd PK, Oh SY, Krans A, He F, Sellier C, Frazer M, Renoux AJ, Chen KC, Scaglione KM, Basrur V, Elenitoba-Johnson K, Vonsattel JP, Louis ED, Sutton MA, Taylor JP, Mills RE, Charlet-Berguerand N, Paulson HL) Neuron 2013 May 08;78(3):440-55 PMID: 23602499 PMCID: PMC3831531 04/23/2013
  • Analysis of the tau-associated proteome reveals that exchange of Hsp70 for Hsp90 is involved in tau degradation. (Thompson AD, Scaglione KM, Prensner J, Gillies AT, Chinnaiyan A, Paulson HL, Jinwal UK, Dickey CA, Gestwicki JE) ACS Chem Biol 2012 Oct 19;7(10):1677-86 PMID: 22769591 PMCID: PMC3477299 07/10/2012
  • Ube2w and ataxin-3 coordinately regulate the ubiquitin ligase CHIP. (Scaglione KM, Zavodszky E, Todi SV, Patury S, Xu P, Rodríguez-Lebrón E, Fischer S, Konen J, Djarmati A, Peng J, Gestwicki JE, Paulson HL) Mol Cell 2011 Aug 19;43(4):599-612 PMID: 21855799 PMCID: PMC3166620 08/23/2011
  • The loop-less tmCdc34 E2 mutant defective in polyubiquitination in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2. (Lass A, Cocklin R, Scaglione KM, Skowyra M, Korolev S, Goebl M, Skowyra D) Cell Div 2011 Mar 31;6:7 PMID: 21453497 PMCID: PMC3080790 04/02/2011
  • Activity and cellular functions of the deubiquitinating enzyme and polyglutamine disease protein ataxin-3 are regulated by ubiquitination at lysine 117. (Todi SV, Scaglione KM, Blount JR, Basrur V, Conlon KP, Pastore A, Elenitoba-Johnson K, Paulson HL) J Biol Chem 2010 Dec 10;285(50):39303-13 PMID: 20943656 PMCID: PMC2998082 10/15/2010
  • Ubiquitination directly enhances activity of the deubiquitinating enzyme ataxin-3. (Todi SV, Winborn BJ, Scaglione KM, Blount JR, Travis SM, Paulson HL) EMBO J 2009 Feb 18;28(4):372-82 PMID: 19153604 PMCID: PMC2646149 01/21/2009
  • The deubiquitinating enzyme ataxin-3, a polyglutamine disease protein, edits Lys63 linkages in mixed linkage ubiquitin chains. (Winborn BJ, Travis SM, Todi SV, Scaglione KM, Xu P, Williams AJ, Cohen RE, Peng J, Paulson HL) J Biol Chem 2008 Sep 26;283(39):26436-43 PMID: 18599482 PMCID: PMC2546540 07/05/2008
  • SCF E3-mediated autoubiquitination negatively regulates activity of Cdc34 E2 but plays a nonessential role in the catalytic cycle in vitro and in vivo. (Scaglione KM, Bansal PK, Deffenbaugh AE, Kiss A, Moore JM, Korolev S, Cocklin R, Goebl M, Kitagawa K, Skowyra D) Mol Cell Biol 2007 Aug;27(16):5860-70 PMID: 17562869 PMCID: PMC1952114 06/15/2007
  • Certain pairs of ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s) synthesize nondegradable forked ubiquitin chains containing all possible isopeptide linkages. (Kim HT, Kim KP, Lledias F, Kisselev AF, Scaglione KM, Skowyra D, Gygi SP, Goldberg AL) J Biol Chem 2007 Jun 15;282(24):17375-86 PMID: 17426036 04/12/2007
  • Release of ubiquitin-charged Cdc34-S - Ub from the RING domain is essential for ubiquitination of the SCF(Cdc4)-bound substrate Sic1. (Deffenbaugh AE, Scaglione KM, Zhang L, Moore JM, Buranda T, Sklar LA, Skowyra D) Cell 2003 Sep 05;114(5):611-22 PMID: 13678584 09/19/2003
  • Last update: 05/02/2017
    jenkins-FCD Prod-130 96200611f8481f0aa4f84230b11dd74d063847a3