Medical College of Wisconsin
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Paula Traktman PhD

Sr Associate Dean, Chairman, Professor

Institution: Medical College of Wisconsin
Department: Microbiology and Molecular Genetics


Research Experience

  • Antibodies, Phospho-Specific
  • Antigens, Viral
  • Antiviral Agents
  • Aphidicolin
  • Casein Kinases
  • Cell Cycle
  • Cell Membrane
  • Cell Membrane Structures
  • Cell Proliferation
  • DNA Helicases
  • DNA Repair
  • DNA Replication
  • DNA Topoisomerases
  • DNA, Viral
  • DNA-Directed DNA Polymerase
  • Dual-Specificity Phosphatases
  • Endoplasmic Reticulum
  • Fertilization in Vitro
  • G1 Phase
  • Gametogenesis
  • Genes, Viral
  • Genetic Vectors
  • Golgi Apparatus
  • Infertility
  • Infertility, Female
  • Infertility, Male
  • Intracellular Membranes
  • Membrane Fusion
  • Membrane Lipids
  • Membrane Proteins
  • Membranes
  • Molecular Biology
  • Molluscipoxvirus
  • Mutation, Missense
  • Nuclear Envelope
  • Oocytes
  • Orthopoxvirus
  • Ovarian Follicle
  • Phosphonoacetic Acid
  • Phosphorylation
  • Plaque Assay
  • Poxviridae
  • Poxviridae Infections
  • Protein Kinase Inhibitors
  • Protein Kinase Inhibitors
  • Protein Kinases
  • Protein Tyrosine Phosphatases, Non-Receptor
  • Protein-Serine-Threonine Kinases
  • S Phase
  • Sertoli Cell-Only Syndrome
  • Signal Transduction
  • Smallpox
  • Smallpox Vaccine
  • Spermatogenesis
  • Spermatogonia
  • Spermine
  • Telomere
  • Testis
  • Vaccinia
  • Vaccinia virus
  • Viral Core Proteins
  • Viral Envelope Proteins
  • Viral Nonstructural Proteins
  • Viral Proteins
  • Viral Structural Proteins
  • Virion
  • Virus Assembly
  • Virus Internalization
  • Virus Replication
  • genetics
  • virology
  • Leadership Positions

  • Professor
    Chairman of Microbiology & Molecular Genetics
    Senior Associate Dean for Research Development
  • Publications

  • Depletion of the protein kinase VRK1 disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes. (Molitor TP, Traktman P) Mol Biol Cell 2014 Mar;25(6):891-903 PMID 24430874
  • Molecular genetic analysis of VRK1 in mammary epithelial cells: depletion slows proliferation in vitro and tumor growth and metastasis in vivo. (Molitor TP, Traktman P) Oncogenesis 2013;2:e48 PMID 23732708
  • Biogenesis of the vaccinia virus membrane: genetic and ultrastructural analysis of the contributions of the A14 and A17 proteins. (Unger B, Mercer J, Boyle KA, Traktman P) J Virol 2013 Jan;87(2):1083-97 PMID 23135725
  • Molecular genetic and biochemical characterization of the vaccinia virus I3 protein, the replicative single-stranded DNA binding protein. (Greseth MD, Boyle KA, Bluma MS, Unger B, Wiebe MS, Soares-Martins JA, Wickramasekera NT, Wahlberg J, Traktman P) J Virol 2012 Jun;86(11):6197-209 PMID 22438556
  • A vaccinia virus-driven interplay between the MKK4/7-JNK1/2 pathway and cytoskeleton reorganization. (Pereira AC, Leite FG, Brasil BS, Soares-Martins JA, Torres AA, Pimenta PF, Souto-Padrón T, Traktman P, Ferreira PC, Kroon EG, Bonjardim CA) J Virol 2012 Jan;86(1):172-84 PMID 22031940
  • Evaluation of the role of the vaccinia virus uracil DNA glycosylase and A20 proteins as intrinsic components of the DNA polymerase holoenzyme. (Boyle KA, Stanitsa ES, Greseth MD, Lindgren JK, Traktman P) J Biol Chem 2011 Jul 15;286(28):24702-13 PMID 21572084
  • Structure/Function analysis of the vaccinia virus F18 phosphoprotein, an abundant core component required for virion maturation and infectivity. (Wickramasekera NT, Traktman P) J Virol 2010 Jul;84(13):6846-60 PMID 20392848
  • Mice deficient in the serine/threonine protein kinase VRK1 are infertile due to a progressive loss of spermatogonia. (Wiebe MS, Nichols RJ, Molitor TP, Lindgren JK, Traktman P) Biol Reprod 2010 Jan;82(1):182-93 PMID 19696012
  • Functional characterization of the vaccinia virus I5 protein. (Unger B, Nichols RJ, Stanitsa ES, Traktman P) Virol J 2008;5:148 PMID 19077320
  • The vaccinia virus gene I2L encodes a membrane protein with an essential role in virion entry. (Nichols RJ, Stanitsa E, Unger B, Traktman P) J Virol 2008 Oct;82(20):10247-61 PMID 18701587
  • Poxviral B1 kinase overcomes barrier to autointegration factor, a host defense against virus replication. (Wiebe MS, Traktman P) Cell Host Microbe 2007 May 17;1(3):187-97 PMID 18005698
  • BAFfled by poxviruses? (Rahman MM, McFadden G) Cell Host Microbe 2007 May 17;1(3):159-60 PMID 18005693
  • Biochemical and genetic analysis of the vaccinia virus d5 protein: Multimerization-dependent ATPase activity is required to support viral DNA replication. (Boyle KA, Arps L, Traktman P) J Virol 2007 Jan;81(2):844-59 PMID 17093187
  • In a nutshell: structure and assembly of the vaccinia virion. (Condit RC, Moussatche N, Traktman P) Adv Virus Res 2006;66:31-124 PMID 16877059
  • The vaccinia-related kinases phosphorylate the N' terminus of BAF, regulating its interaction with DNA and its retention in the nucleus. (Nichols RJ, Wiebe MS, Traktman P) Mol Biol Cell 2006 May;17(5):2451-64 PMID 16495336
  • Vaccinia virus uracil DNA glycosylase interacts with the A20 protein to form a heterodimeric processivity factor for the viral DNA polymerase. (Stanitsa ES, Arps L, Traktman P) J Biol Chem 2006 Feb 10;281(6):3439-51 PMID 16326701
  • Genetic and cell biological characterization of the vaccinia virus A30 and G7 phosphoproteins. (Mercer J, Traktman P) J Virol 2005 Jun;79(11):7146-61 PMID 15890954
  • Cell biological and functional characterization of the vaccinia virus F10 kinase: implications for the mechanism of virion morphogenesis. (Punjabi A, Traktman P) J Virol 2005 Feb;79(4):2171-90 PMID 15681420
  • Poxvirus Bioinformatics Resource Center: a comprehensive Poxviridae informational and analytical resource. (Lefkowitz EJ, Upton C, Changayil SS, Buck C, Traktman P, Buller RM) Nucleic Acids Res 2005 Jan 1;33(Database issue):D311-6 PMID 15608205
  • Vaccinia virus morphogenesis: a13 phosphoprotein is required for assembly of mature virions. (Unger B, Traktman P) J Virol 2004 Aug;78(16):8885-901 PMID 15280497
  • Methods for analysis of poxvirus DNA replication. (Traktman P, Boyle K) Methods Mol Biol 2004;269:169-86 PMID 15114016
  • Members of a novel family of mammalian protein kinases complement the DNA-negative phenotype of a vaccinia virus ts mutant defective in the B1 kinase. (Boyle KA, Traktman P) J Virol 2004 Feb;78(4):1992-2005 PMID 14747564
  • Characterization of three paralogous members of the Mammalian vaccinia related kinase family. (Nichols RJ, Traktman P) J Biol Chem 2004 Feb 27;279(9):7934-46 PMID 14645249
  • Genetic analysis of the vaccinia virus I6 telomere-binding protein uncovers a key role in genome encapsidation. (Grubisha O, Traktman P) J Virol 2003 Oct;77(20):10929-42 PMID 14512543
  • Investigation of structural and functional motifs within the vaccinia virus A14 phosphoprotein, an essential component of the virion membrane. (Mercer J, Traktman P) J Virol 2003 Aug;77(16):8857-71 PMID 12885904
  • The vaccinia virus I3L gene product is localized to a complex endoplasmic reticulum-associated structure that contains the viral parental DNA. (Welsch S, Doglio L, Schleich S, Krijnse Locker J) J Virol 2003 May;77(10):6014-28 PMID 12719593
  • Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation. (Lerch-Gaggl A, Haque J, Li J, Ning G, Traktman P, Duncan SA) J Biol Chem 2002 Nov 22;277(47):45347-55 PMID 12237316
  • Clustered charge-to-alanine mutagenesis of the vaccinia virus A20 gene: temperature-sensitive mutants have a DNA-minus phenotype and are defective in the production of processive DNA polymerase activity. (Punjabi A, Boyle K, DeMasi J, Grubisha O, Unger B, Khanna M, Traktman P) J Virol 2001 Dec;75(24):12308-18 PMID 11711621
  • The A20R protein is a stoichiometric component of the processive form of vaccinia virus DNA polymerase. (Klemperer N, McDonald W, Boyle K, Unger B, Traktman P) J Virol 2001 Dec;75(24):12298-307 PMID 11711620
  • Vaccinia virus telomeres: interaction with the viral I1, I6, and K4 proteins. (DeMasi J, Du S, Lennon D, Traktman P) J Virol 2001 Nov;75(21):10090-105 PMID 11581377
  • Vaccinia virus blocks gamma interferon signal transduction: viral VH1 phosphatase reverses Stat1 activation. (Najarro P, Traktman P, Lewis JA) J Virol 2001 Apr;75(7):3185-96 PMID 11238845
  • Elucidating the essential role of the A14 phosphoprotein in vaccinia virus morphogenesis: construction and characterization of a tetracycline-inducible recombinant. (Traktman P, Liu K, DeMasi J, Rollins R, Jesty S, Unger B) J Virol 2000 Apr;74(8):3682-95 PMID 10729144
  • Clustered charge-to-alanine mutagenesis of the vaccinia virus H5 gene: isolation of a dominant, temperature-sensitive mutant with a profound defect in morphogenesis. (DeMasi J, Traktman P) J Virol 2000 Mar;74(5):2393-405 PMID 10666270
  • The vaccinia virus A4OR gene product is a nonstructural, type II membrane glycoprotein that is expressed at the cell surface. (Wilcock D, Duncan SA, Traktman P, Zhang WH, Smith GL) J Gen Virol 1999 Aug;80 ( Pt 8):2137-48 PMID 10466813
  • Tyrosine phosphorylation of A17 during vaccinia virus infection: involvement of the H1 phosphatase and the F10 kinase. (Derrien M, Punjabi A, Khanna M, Grubisha O, Traktman P) J Virol 1999 Sep;73(9):7287-96 PMID 10438817
  • Characterization of the single-stranded DNA binding protein encoded by the vaccinia virus I3 gene. (Rochester SC, Traktman P) J Virol 1998 Apr;72(4):2917-26 PMID 9525612
  • The vaccinia virus I1 protein is essential for the assembly of mature virions. (Klemperer N, Ward J, Evans E, Traktman P) J Virol 1997 Dec;71(12):9285-94 PMID 9371587
  • Characterization of a processive form of the vaccinia virus DNA polymerase. (McDonald WF, Klemperer N, Traktman P) Virology 1997 Jul 21;234(1):168-75 PMID 9234958
  • Cytotoxic T lymphocyte responses to proteins encoded by heterologous transgenes transferred in vivo by adenoviral vectors. (Song W, Kong HL, Traktman P, Crystal RG) Hum Gene Ther 1997 Jul 1;8(10):1207-17 PMID 9215738
  • Vaccinia virus DNA replication: two hundred base pairs of telomeric sequence confer optimal replication efficiency on minichromosome templates. (Du S, Traktman P) Proc Natl Acad Sci U S A 1996 Sep 3;93(18):9693-8 PMID 8790393
  • The dual-specificity phosphatase encoded by vaccinia virus, VH1, is essential for viral transcription in vivo and in vitro. (Liu K, Lemon B, Traktman P) J Virol 1995 Dec;69(12):7823-34 PMID 7494294
  • Temperature-sensitive mutants with lesions in the vaccinia virus F10 kinase undergo arrest at the earliest stage of virion morphogenesis. (Traktman P, Caligiuri A, Jesty SA, Liu K, Sankar U) J Virol 1995 Oct;69(10):6581-7 PMID 7666563
  • The vaccinia virus D5 protein, which is required for DNA replication, is a nucleic acid-independent nucleoside triphosphatase. (Evans E, Klemperer N, Ghosh R, Traktman P) J Virol 1995 Sep;69(9):5353-61 PMID 7636979
  • Identification and characterization of the orf virus type I topoisomerase. (Klemperer N, Lyttle DJ, Tauzin D, Traktman P, Robinson AJ) Virology 1995 Jan 10;206(1):203-15 PMID 7831775
  • Vaccinia virus DNA polymerase. In vitro analysis of parameters affecting processivity. (McDonald WF, Traktman P) J Biol Chem 1994 Dec 9;269(49):31190-7 PMID 7983061
  • Voda guiding catheter is superior to amplatz guiding catheter for performing left circumflex PTCA. (Mehta S, Margolis JR, Traktman M, Kramer B, Trautwein R, Gasso J, Nader R) J Invasive Cardiol 1994 Sep;6(7):229-33 PMID 10155073
  • Overexpression and purification of the vaccinia virus DNA polymerase. (McDonald WF, Traktman P) Protein Expr Purif 1994 Aug;5(4):409-21 PMID 7950389
  • Biochemical analysis of mutant alleles of the vaccinia virus topoisomerase I carrying targeted substitutions in a highly conserved domain. (Klemperer N, Traktman P) J Biol Chem 1993 Jul 25;268(21):15887-99 PMID 8393454
  • Genetic characterization of the vaccinia virus DNA polymerase: cytosine arabinoside resistance requires a variable lesion conferring phosphonoacetate resistance in conjunction with an invariant mutation localized to the 3'-5' exonuclease domain. (Taddie JA, Traktman P) J Virol 1993 Jul;67(7):4323-36 PMID 8389930
  • Vaccinia virus B1 kinase: phenotypic analysis of temperature-sensitive mutants and enzymatic characterization of recombinant proteins. (Rempel RE, Traktman P) J Virol 1992 Jul;66(7):4413-26 PMID 1602551
  • Transient expression of the vaccinia virus DNA polymerase is an intrinsic feature of the early phase of infection and is unlinked to DNA replication and late gene expression. (McDonald WF, Crozel-Goudot V, Traktman P) J Virol 1992 Jan;66(1):534-47 PMID 1727498
  • Characterization of vaccinia virus DNA replication mutants with lesions in the D5 gene. (Evans E, Traktman P) Chromosoma 1992;102(1 Suppl):S72-82 PMID 1291243
  • Genetic characterization of the vaccinia virus DNA polymerase: identification of point mutations conferring altered drug sensitivities and reduced fidelity. (Taddie JA, Traktman P) J Virol 1991 Feb;65(2):869-79 PMID 1898973
  • Determination of functional effects of mutations in the steroid 21-hydroxylase gene (CYP21) using recombinant vaccinia virus. (Tusie-Luna MT, Traktman P, White PC) J Biol Chem 1990 Dec 5;265(34):20916-22 PMID 2249999
  • Poxviruses: an emerging portrait of biological strategy. (Traktman P) Cell 1990 Aug 24;62(4):621-6 PMID 1974828
  • Identification and DNA sequence of the Shope fibroma virus DNA topoisomerase gene. (Upton C, Opgenorth A, Traktman P, McFadden G) Virology 1990 Jun;176(2):439-47 PMID 2161144
  • Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W. (Nocka K, Tan JC, Chiu E, Chu TY, Ray P, Traktman P, Besmer P) EMBO J 1990 Jun;9(6):1805-13 PMID 1693331
  • Temperature-sensitive vaccinia virus mutants identify a gene with an essential role in viral replication. (Rempel RE, Anderson MK, Evans E, Traktman P) J Virol 1990 Feb;64(2):574-83 PMID 2296077
  • The dominant W42 spotting phenotype results from a missense mutation in the c-kit receptor kinase. (Tan JC, Nocka K, Ray P, Traktman P, Besmer P) Science 1990 Jan 12;247(4939):209-12 PMID 1688471
  • The enzymology of poxvirus DNA replication. (Traktman P) Curr Top Microbiol Immunol 1990;163:93-123 PMID 2242683
  • Vaccinia virus encodes an essential gene with strong homology to protein kinases. (Traktman P, Anderson MK, Rempel RE) J Biol Chem 1989 Dec 25;264(36):21458-61 PMID 2600076
  • Genetic evidence for involvement of vaccinia virus DNA-dependent ATPase I in intermediate and late gene expression. (Künzi MS, Traktman P) J Virol 1989 Sep;63(9):3999-4010 PMID 2527312
  • Molecular genetic analysis of vaccinia virus DNA polymerase mutants. (Traktman P, Kelvin M, Pacheco S) J Virol 1989 Feb;63(2):841-6 PMID 2911123
  • Decreased TRH receptor mRNA activity precedes homologous downregulation: assay in oocytes. (Oron Y, Straub RE, Traktman P, Gershengorn MC) Science 1987 Dec 4;238(4832):1406-8 PMID 2825350
  • Molecular genetic analysis of a vaccinia virus gene with an essential role in DNA replication. (Evans E, Traktman P) J Virol 1987 Oct;61(10):3152-62 PMID 3041037
  • Vaccinia virus encapsidates a novel topoisomerase with the properties of a eucaryotic type I enzyme. (Shaffer R, Traktman P) J Biol Chem 1987 Jul 5;262(19):9309-15 PMID 3036853
  • Transcriptional mapping of the DNA polymerase gene of vaccinia virus. (Traktman P, Sridhar P, Condit RC, Roberts BE) J Virol 1984 Jan;49(1):125-31 PMID 6317886
  • Protease bypass of temperature-sensitive murine leukemia virus maturation mutants. (Traktman P, Baltimore D) J Virol 1982 Dec;44(3):1039-46 PMID 6184485
  • Isolation and properties of Moloney murine leukemia virus mutants: use of a rapid assay for release of virion reverse transcriptase. (Goff S, Traktman P, Baltimore D) J Virol 1981 Apr;38(1):239-48 PMID 6165830
  • N-Tropic variants obtained after co-infection with N- and B-tropic murine leukemia viruses. (Hopkins N, Traktman P, Whalen K) J Virol 1976 Apr;18(1):324-31 PMID 176467
  • Grants and Awards

  • Vaccinia DNA Replication (#9203259 05/01/2004 - 09/25/2009) Sponsor: NIH Allergy and Infectious Diseases
  • RNAi-mediated Identification of Essential Poxvirus Genes (#9203213 03/01/2004 - 02/28/2005) Sponsor: University of Chicago
  • Vaccinia DNA Replication (#9207358 03/01/2012 - 02/28/2017) Sponsor: NIH Allergy and Infectious Diseases
  • Vaccinia Encoded Kinases and Phosphatases (#9203731 07/01/2005 - 03/31/2011) Sponsor: NIH Allergy and Infectious Diseases
  • Role for Vaccinia B1 Kinase in Translational Regulation (#9203215 03/01/2004 - 02/28/2005) Sponsor: University of Chicago
  • Cancer Center Chemoprevention Program (#9520180 11/01/2010 - 10/31/2016) PI of Investigation of the Role of the VRK1 Protein Kinase in Human Cancer (#5520272)