Effects of serum on (-)-gossypol-suppressed growth in human prostate cancer cells. Anticancer Res 2006;26(5A):3613-20
Date
11/11/2006Pubmed ID
17094491Scopus ID
2-s2.0-33750744420 (requires institutional sign-in at Scopus site) 22 CitationsAbstract
BACKGROUND: Gossypol, a natural polyphenolic compound present in cottonseeds, possesses antiproliferative and pro-apoptotic effects in in vivo and in vitro models. There are two enantiomers, (+)-gossypol and (-)-gossypol, the latter being a more potent inhibitor of cancer cell growth. Here, the effect of bovine serum albumin (BSA) and dextran-coated charcoal-treated fetal bovine serum (DCC-FBS)-containing medium on the ability of (-)-gossypol to inhibit the growth of human prostate cancer cells was studied.
MATERIALS AND METHODS: BSA- and DCC-FBS-supplemented medium were used to examine the influence of serum proteins on the antiproliferative effects of (-)-gossypol in DU-145 cells, a human prostate cancer cell line. The viability of the DU-145 cells was determined by CellTiter 96 Aqueous assay. The expressions of mRNA and protein for the cell cycle regulators, cyclin-D1, Rb, CDK, p21 and TGF-beta, were determined by RT-PCR and Western blot analyses, respectively.
RESULTS: (-)-Gossypol caused growth suppression of the DU-145 cells. In comparison with BSA-supplemented medium, DCC-FBS blocked the antiproliferative effects of (-)-gossypol at 1 and 2.5 microM, but not at 5 microM. Furthermore, (-)-gossypol treatment down-regulated cyclin-D1, Rb, CDK4 and CDK6, and up-regulated p21 and TGF-beta1 at the mRNA and/or protein levels.
CONCLUSION: The data suggested that (-)-gossypol-suppressed prostate cancer cell growth may be influenced through cell cycle regulators, which may lead to better prognosis. We further speculate that (-)-gossypol might serve as a chemotherapeutic agent for human prostate cancer patients.
Author List
Huang YW, Wang LS, Chang HL, Ye W, Sugimoto Y, Dowd MK, Wan PJ, Lin YCMESH terms used to index this publication - Major topics in bold
AnimalsBlotting, Western
Cattle
Cell Survival
Charcoal
Cyclin D1
Cyclin-Dependent Kinase 4
Cyclin-Dependent Kinase 6
Cyclin-Dependent Kinase Inhibitor p21
Dextrans
Gossypol
Humans
Male
Neoplasms, Hormone-Dependent
Prostatic Neoplasms
RNA, Messenger
RNA, Neoplasm
Retinoblastoma Protein
Reverse Transcriptase Polymerase Chain Reaction
Serum
Serum Albumin, Bovine
Transforming Growth Factor beta1
Tumor Cells, Cultured