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Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases. J Vis Exp 2016 Mar 11(109)

Date

03/31/2016

Pubmed ID

27023389

Pubmed Central ID

PMC4828227

DOI

10.3791/53577

Abstract

The retinal pigment epithelium (RPE) is juxtaposed to the overlying sensory retina, and supports the function of the visual system. Among the tasks performed by the RPE are phagocytosis and processing of outer photoreceptor segments through lysosome-derived organelles. These degradation products, stored and referred to as lipofuscin granules, are composed partially of bisretinoids, which have broad fluorescence absorption and emission spectra that can be detected clinically as fundus autofluorescence with confocal scanning laser ophthalmoscopy (cSLO). Lipofuscin accumulation is associated with increasing age, but is also found in various patterns in both acquired and inherited degenerative diseases of the retina. Thus, studying its pattern of accumulation and correlating such patterns with changes in the overlying sensory retina are essential to understanding the pathophysiology and progression of retinal disease. Here, we describe a technique employed by our lab and others that uses cSLO in order to quantify the level of RPE lipofuscin in both healthy and diseased eyes.

Author List

Armenti ST, Greenberg JP, Smith RT

Author

Jonathan P. Greenberg MD Instructor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Female
Fundus Oculi
Geographic Atrophy
Humans
Lipofuscin
Ophthalmoscopy
Optical Imaging
Retinal Pigment Epithelium
Tomography, Optical Coherence
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