Rat adipose ob mRNA levels in states of altered circulating glucose and insulin. Biochem Biophys Res Commun 1996 Mar 27;220(3):520-5
Date
03/27/1996Pubmed ID
8607798DOI
10.1006/bbrc.1996.0437Scopus ID
2-s2.0-0029977866 (requires institutional sign-in at Scopus site) 37 CitationsAbstract
We measured levels of ob mRNA in epididymal fat pads of rats exposed to manipulations designed to alter circulating insulin and glycemia. Changes in ob mRNA were compared to alterations in GLUT-4 glucose transporter mRNA, which is known to be regulated under these conditions. 48 h fasting decreased GLUT-4 mRNA to 23% of control with restoration beginning by 6 h refeeding and full restoration at 24 h. In contrast, ob mRNA decreased less markedly to 47% of control with only partial restoration by 24 h. Two days of streptozocin (STZ)-diabetes (glucose > 400 mg/100 ml) decreased GLUT-4 mRNA to 8% of control with restoration by two days of S.C. insulin. In contrast, ob mRNA decreased to 42% of control and was not restored by insulin. Six days of insulin administration to normal rats under conditions of ad lib. feeding, but without otherwise preventing the blood glucose from decreasing, resulted in no significant change in levels of either ob or GLUT-4 mRNA.
Author List
Sivitz WI, Bailey HL, Donohoue PMESH terms used to index this publication - Major topics in bold
Adipose TissueAnimals
Base Sequence
Blood Glucose
DNA Primers
Diabetes Mellitus, Experimental
Eating
Epididymis
Fasting
Gene Expression Regulation
Glucose Transporter Type 4
Humans
Insulin
Leptin
Male
Molecular Sequence Data
Monosaccharide Transport Proteins
Muscle Proteins
Obesity
Protein Biosynthesis
RNA, Messenger
Rats
Rats, Sprague-Dawley
Recombinant Proteins
Reference Values
Time Factors
