Faculty Collaboration Database

Diagnosis of Bernard-Soulier syndrome and Glanzmann's thrombasthenia with a monoclonal assay on whole blood. J Clin Invest 1983 Feb;71(2):385-9

Date

02/01/1983

Pubmed ID

6822670

Pubmed Central ID

PMC436878

DOI

10.1172/jci110780

Abstract

Two hereditary platelet disorders, Bernard-Soulier syndrome and Glanzmann's thrombasthenia, are characterized by selective deficiencies of platelet membrane glycoproteins. Murine monoclonal antibodies were developed against platelet membrane glycoprotein Ib and against the glycoprotein IIb/IIIa complex. A rapid whole blood assay for the deficiency of these glycoproteins was developed and used to study whole blood samples from six patients with Glanzmann's thrombasthenia and three patients with Bernard-Soulier syndrome. Patients with type I and type II Glanzmann's thrombasthenia were easily detectable with this assay. This permits the diagnosis of these disorders on 200 microliters of whole blood within 2 h of blood sampling.

Author List

Montgomery RR, Kunicki TJ, Taves C, Pidard D, Corcoran M

Author

Robert R. Montgomery MD Adjunct Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Monoclonal
Antibody Specificity
Autoradiography
Blood Platelet Disorders
Cell Membrane
Glycoproteins
Humans
Mice
Mice, Inbred BALB C