Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system. Methods Mol Biol 2006;337:3-13
Date
08/26/2006Pubmed ID
16929934DOI
10.1385/1-59745-095-2:3Scopus ID
2-s2.0-39049190181 (requires institutional sign-in at Scopus site) 15 CitationsAbstract
Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells.
Author List
Staruschenko A, Booth RE, Pochynyuk O, Stockand JD, Tong QMESH terms used to index this publication - Major topics in bold
AnimalsCHO Cells
Cricetinae
Cricetulus
Epithelial Sodium Channels
Gene Expression
Humans
Membrane Potentials
Mice
Patch-Clamp Techniques
Recombinant Proteins