Cloning, characterization, and genetic mapping of the rat type 2 angiotensin II receptor gene. Hypertension 1995 Dec;26(6 Pt 1):998-1002
Date
12/01/1995Pubmed ID
7490161DOI
10.1161/01.hyp.26.6.998Scopus ID
2-s2.0-0028839627 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
The renin-angiotensin system plays an important role in blood pressure homeostasis, but the contribution of the type 2 angiotensin II receptor (AT2R) is still unclear. The reports that the AT2R gene has been mapped to the X chromosome in human and rat and the previous report of a gene, Bp3, on the X chromosome responsible for an increase in blood pressure have suggested that the rat AT2R gene (Agtr2) could be this gene. To elucidate whether Agtr2 is Bp3, Agtr2 was cloned. A simple sequence repeat in the 3'-flanking region of this gene was identified and used as a genetic marker to map Agtr2 to the X chromosome at 18.1 cM distal to the androgen receptor locus. This map position is outside the confidence interval reported for Bp3, demonstrating that Agtr2 cannot be Bp3. However, these data will enhance the research into the AT2R biology as well as the study of the X chromosome.
Author List
Koike G, Winer ES, Horiuchi M, Brown DM, Szpirer C, Dzau VJ, Jacob HJMESH terms used to index this publication - Major topics in bold
AllelesAngiotensin II
Animals
Base Sequence
Chromosome Mapping
Cloning, Molecular
Genes
Genetic Markers
Genomic Library
Hypertension
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Rats
Rats, Inbred SHR
Rats, Inbred Strains
Rats, Inbred WKY
Rats, Sprague-Dawley
Receptors, Angiotensin
Transcription, Genetic
X Chromosome