Human type 2 angiotensin II receptor gene: cloned, mapped to the X chromosome, and its mRNA is expressed in the human lung. Biochem Biophys Res Commun 1994 Sep 30;203(3):1842-50
Date
09/30/1994Pubmed ID
7945336DOI
10.1006/bbrc.1994.2402Scopus ID
2-s2.0-0028148285 (requires institutional sign-in at Scopus site) 102 CitationsAbstract
The human type 2 angiotensin II receptor (AT2R) gene was isolated from a human genomic DNA library of adult female leukocytes. The putative coding region of the human AT2R gene is intronless. The primary structure of the human AT2R deduced from the nucleotide sequence of this putative coding region contains 363 amino acid residues and is highly homologous to the sequences of the rat and mouse AT2R. The human AT2R gene was mapped to the X chromosome using chromosomal assignment analysis with a human/rodent somatic cell hybrid panel. Northern blot analysis demonstrated a 3.0 kb mRNA in the human fetal lung and kidney and the human adult lung. These data and probes should enhance research in human AT2R biology.
Author List
Koike G, Horiuchi M, Yamada T, Szpirer C, Jacob HJ, Dzau VJMESH terms used to index this publication - Major topics in bold
AdultAmino Acid Sequence
Angiotensin II
Animals
Base Sequence
Blotting, Southern
Cell Line
Chromosome Mapping
Cloning, Molecular
DNA Primers
Female
Gene Expression
Genomic Library
Humans
Hybrid Cells
Kinetics
Leukocytes
Lung
Mice
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger
Rats
Receptors, Angiotensin
Sequence Homology, Amino Acid
Transfection
X Chromosome
