Medical College of Wisconsin
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Preferential amplification of the paternal allele in neuroblastomas with N-myc amplification. Prog Clin Biol Res 1994;385:43-9 PMID: 7972236

Pubmed ID

7972236

Abstract

There is increasing evidence that imprinting plays an important role in influencing the parental origin of genes involved in cancer-specific rearrangements. In advanced stage neuroblastomas, both allelic loss of the short arm of chromosome 1 (1p) and amplification of the proto-oncogene N-myc are often seen. Therefore, we analyzed 22 human neuroblastomas with N-myc amplification to determine the parental origin of the N-myc allele that is amplified and the 1p allele that is deleted. We used at least three polymorphisms for both the 1p and the N-myc locus to analyze blood and tumor samples from neuroblastoma patients, as well as blood samples from their parents. We determined that the paternal allele of N-myc was amplified in 12 of 15 informative cases (P = 0.02), and the paternal allele on 1p was lost in 6 of 11 informative cases (P > 0.2). These results suggest that parental imprinting does not appear to affect the 1p allele that is deleted, at least in the cases that we have examined. However, imprinting has an important influence on determining the N-myc allele that is amplified in these tumors, suggesting that the paternal allele is either more highly expressed or more likely to undergo amplification.

Author List

Cheng JM, Hiemstra JL, Schneider SS, Kaufman BA, Naumova A, Cheung NK, Cohn SL, Diller L, Sapienza C, Brodeur GM

Author

Bruce A. Kaufman MD Professor in the Neurosurgery department at Medical College of Wisconsin




Scopus

2-s2.0-0028247759   3 Citations

MESH terms used to index this publication - Major topics in bold

Alleles
Blotting, Southern
Chromosome Deletion
Chromosomes, Human, Pair 1
DNA, Neoplasm
Female
Gene Amplification
Genes, myc
Genomic Imprinting
Humans
Male
Neuroblastoma
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
jenkins-FCD Prod-336 69ef4a6b262d135130251597d5d39873903802b5