A canine model of thrombin-induced coronary artery thrombosis: effects of intracoronary streptokinase on regional myocardial blood flow, contractile function, and infarct size. J Pharmacol Methods 1987 Dec;18(4):305-18
Date
12/01/1987Pubmed ID
3695539DOI
10.1016/0160-5402(87)90062-3Scopus ID
2-s2.0-0023205266 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
The present investigation was undertaken to produce and to validate a canine model of experimental coronary artery thrombosis. In anesthetized, open-chest dogs, thrombin (150-200 U) was injected through an intracoronary catheter proximal to a brief (1 min) left anterior descending coronary artery occlusion. After 1 min, the mechanical occlusion was released, but no reperfusion occurred. Intracoronary thrombus was limited to the large coronary artery proximal to the site of mechanical occlusion. In two groups of dogs (n = 8), 15 min following thrombosis, vehicle (saline) or streptokinase (20,000 IU) was administered as an intracoronary bolus followed by an intracoronary infusion for 1 hr. None of the vehicle-treated dogs demonstrated antegrade coronary flow for up to 4 hr following intracoronary thrombin, whereas seven of eight had return of antegrade flow among streptokinase-treated dogs. Following streptokinase, thrombus size was reduced from control (25.1 +/- 5.3 mg versus 1.8 +/- 1.3 mg), perfusion to the subepicardium, midmyocardium, and subendocardium was increased, and infarct size was reduced. The results of the present study indicate the usefulness and reproducibility of this model in evaluating the beneficial actions of thrombolytic therapy.
Author List
Warltier DC, Lamping KA, Pelc LR, Gross GJMESH terms used to index this publication - Major topics in bold
AnimalsCoronary Circulation
Coronary Disease
Coronary Thrombosis
Disease Models, Animal
Dogs
Female
Male
Myocardial Contraction
Myocardial Infarction
Perfusion
Risk Factors
Streptokinase
Thrombin
