Endothelin enhances adrenocorticotropin-stimulated aldosterone release from cultured bovine adrenal cells. Endocrinology 1990 Apr;126(4):1860-6
Date
04/01/1990Pubmed ID
2156668DOI
10.1210/endo-126-4-1860Scopus ID
2-s2.0-0025361685 (requires institutional sign-in at Scopus site) 68 CitationsAbstract
Endothelin (ET) is an endothelial cell-derived peptide with profound hemodynamic effects. We investigated the effects of ET on steroid release from bovine adrenal cortical cells. ET-1 (10(-11)-10(-6) M) did not alter basal aldosterone release from adrenal glomerulosa cells. However, both angiotensin-II (AII)- and ACTH-stimulated aldosterone release from these cells. ET-1 enhanced ACTH-stimulated aldosterone release over a range of concentrations. This enhancement was observed at a threshold of 0.1 nM ET-1. Similar responses were observed in incubations of 1- and 2-h duration. In contrast, AII-stimulated aldosterone release was not altered by ET-1. ET-1 also enhanced aldosterone release in cells stimulated with 8-bromo-cAMP. All increased cytoplasmic Ca2+ concentrations in fura-2-loaded cells; however, ET-1 alone or with ACTH had no effect. In addition, chronic treatment of cells with ACTH for 2 days caused an enhanced release of aldosterone in cells acutely treated with ET-1 compared to cells not acutely treated (62% over chronic ACTH control; P less than 0.05). However, chronic treatment of ET-1 did not alter aldosterone secretion in the acute incubation compared to that in chronic controls. Thus, both acute and chronic exposures to ACTH sensitize glomerulosa cells to stimulation by ET-1. ET-1 did not alter basal or ACTH-stimulated cortisol release from adrenal fasciculata cells. Thus, the actions of ET-1 appear to be specific for glomerulosa cells. These data indicate that ET may exert a direct effect on adrenal glomerulosa cells to enhance ACTH-stimulated aldosterone release.
Author List
Rosolowsky LJ, Campbell WBAuthor
William B. Campbell PhD Professor in the Pharmacology and Toxicology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
8-Bromo Cyclic Adenosine MonophosphateAdrenal Glands
Adrenocorticotropic Hormone
Aldosterone
Angiotensin II
Animals
Calcium
Cattle
Cells, Cultured
Endothelins
Endothelium, Vascular
Female
Granulosa Cells
Hydrocortisone
Peptides