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Adaptive optics optical coherence tomography at 120,000 depth scans/s for non-invasive cellular phenotyping of the living human retina. Opt Express 2009 Oct 26;17(22):19382-400 PMID: 19997159 PMCID: PMC3474252

Pubmed ID

19997159

DOI

10.1364/OE.17.019382

Abstract

This paper presents a successful combination of ultra-high speed (120,000 depth scans/s), ultra-high resolution optical coherence tomography with adaptive optics and an achromatizing lens for compensation of monochromatic and longitudinal chromatic ocular aberrations, respectively, allowing for non-invasive volumetric imaging in normal and pathologic human retinas at cellular resolution. The capability of this imaging system is demonstrated here through preliminary studies by probing cellular intraretinal structures that have not been accessible so far with in vivo, non-invasive, label-free imaging techniques, including pigment epithelial cells, micro-vasculature of the choriocapillaris, single nerve fibre bundles and collagenous plates of the lamina cribrosa in the optic nerve head. In addition, the volumetric extent of cone loss in two colour-blinds could be quantified for the first time. This novel technique provides opportunities to enhance the understanding of retinal pathogenesis and early diagnosis of retinal diseases.

Author List

Torti C, Povazay B, Hofer B, Unterhuber A, Carroll J, Ahnelt PK, Drexler W

Author

Joseph J. Carroll PhD Director, Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin




Scopus

2-s2.0-70749093526   90 Citations

MESH terms used to index this publication - Major topics in bold

Equipment Design
Equipment Failure Analysis
Humans
Image Enhancement
Lenses
Retina
Retinal Diseases
Retinoscopes
Tomography, Optical Coherence
jenkins-FCD Prod-353 9ccd8489072cb19f5b9f808bb23ed672c582f41e