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Detecting axon damage in spinal cord from a mouse model of multiple sclerosis. Neurobiol Dis 2006 Mar;21(3):626-32 PMID: 16298135

Pubmed ID

16298135

Abstract

In the current study, the feasibility and reproducibility of in vivo diffusion tensor imaging (DTI) of the spinal cord in normal mice are illustrated followed by its application to mice with experimental allergic encephalomyelitis (EAE) to detect and differentiate axon and myelin damage. Axial diffusivity, describing water movement along the axonal fiber tract, in all regions of spinal cord white matter from EAE-affected C57BL/6 mice was significantly decreased compared to normal mice, whereas there was no statistically significant change in radial diffusivity, describing water movement across the fiber tract. Furthermore, a direct comparison between DTI and histology from a single mouse demonstrated a decrease in axial diffusivity that was supported by widespread staining of antibody against beta-amyloid precursor protein. Regionally elevated radial diffusivity corresponded with locally diminished Luxol fast blue staining in the same tissue from the EAE mouse cord. Our findings suggest that axonal damage is more widespread than myelin damage in the spinal cord white matter of mice with EAE and that in vivo DTI may provide a sensitive and specific measure of white matter injury.

Author List

Kim JH, Budde MD, Liang HF, Klein RS, Russell JH, Cross AH, Song SK

Author

Matthew Budde PhD Associate Professor in the Neurosurgery department at Medical College of Wisconsin




Scopus

2-s2.0-33244488050   181 Citations

MESH terms used to index this publication - Major topics in bold

Animals
Axons
Diffusion
Diffusion Magnetic Resonance Imaging
Encephalomyelitis, Autoimmune, Experimental
Female
Mice
Mice, Inbred C57BL
Multiple Sclerosis
Myelin Sheath
Reproducibility of Results
Spinal Cord
jenkins-FCD Prod-271 16cb5dcaa6d2cfe4986875aea432578e3ad30554