Evaluation of the pulmonary immune response by analysis of bronchoalveolar fluids obtained by serial lung lavage. Int Arch Allergy Appl Immunol 1983;71(2):173-7
Date
01/01/1983Pubmed ID
6840873DOI
10.1159/000233383Scopus ID
2-s2.0-0020623487 (requires institutional sign-in at Scopus site) 7 CitationsAbstract
Lavage fluids were obtained from Beagle dogs by serial bronchoalveolar lavage from immunized and control lung lobes 3-21 days after local deposition of sheep red blood cells. Results showed that immunized lobes contained more total protein, albumin, IgG and antigen-specific antibody than did unimmunized control lobes or lobes that received autologous red cells in saline. Antigen-specific antibody was present in the lavage fluid from 7 through 21 days after immunization. Mercaptoethanol treatment of lavage fluid had little effect on the antibody titer, suggesting that IgG, and possibly IgA, were the predominant immunoglobulin classes in the antigen-specific antibody pool throughout the course of the serial lavage study. The data suggest that most of the antigen-specific antibody was made locally by cells in the lung.
Author List
Hill JO, Bice DE, Harris DL, Muggenburg BAMESH terms used to index this publication - Major topics in bold
AlbuminsAnimals
Antibody Specificity
Dogs
Erythrocytes
Immunoglobulin A
Immunoglobulin G
Inflammation
Isoantibodies
Lung
Pulmonary Alveoli
Therapeutic Irrigation
