In vivo expression of a TCR antagonist: T cells escape central tolerance but are antagonized in the periphery. J Immunol 1998 Jul 01;161(1):128-37
Date
07/01/1998Pubmed ID
9647216Scopus ID
2-s2.0-0031831379 (requires institutional sign-in at Scopus site) 30 CitationsAbstract
Transgenic 3.L2 T cells are stimulated by Hb(64-76)/I-Ek and are positively selected on I-Ek plus self-peptides. To this pool of self-peptides we have added a single, well-defined 3.L2 TCR antagonist (A72) in vivo. We find that mice expressing both the 3.L2 TCR and A72 have a minimal loss of T cells expressing the clonotypic TCR in the thymus and spleen. Importantly, the proliferative response of 3.L2 x A72 splenocytes is significantly reduced compared with splenocytes from 3.L2 mice. This reduced response can be attributed to peripheral antagonism. Thus we have identified a new class of self-ligands whose predominant effect is constitutive peripheral antagonism rather than negative selection. The net effect of these ligands is to avoid potential self-reactivity while maintaining as large a repertoire as possible.
Author List
Williams CB, Vidal K, Donermeyer D, Peterson DA, White JM, Allen PMAuthor
Calvin B. Williams MD, PhD Chief, Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAntigen-Presenting Cells
Chickens
Clone Cells
Hemoglobins
Histocompatibility Antigens Class II
Immune Tolerance
Ligands
Lymphocyte Activation
Lymphocyte Count
Mice
Mice, Inbred AKR
Mice, Inbred C57BL
Mice, Transgenic
Muramidase
Peptide Fragments
Receptors, Antigen, T-Cell
Recombinant Fusion Proteins
Spleen
T-Lymphocyte Subsets
Thymus Gland
Transgenes