Faculty Collaboration Database

Detection of drug-dependent antibodies by the 51Cr platelet lysis test: documentation of immune thrombocytopenia induced by diphenylhydantoin, diazepam, and sulfisoxazole. Am J Hematol 1977;2(1):65-72

Date

01/01/1977

Pubmed ID

868869

DOI

10.1002/ajh.2830020109

Scopus ID

2-s2.0-0017620127 (requires institutional sign-in at Scopus site)   59 Citations

Abstract

Sudden, severe thrombocytopenia developed in each of three patients receiving diphenylhydantion, diazepam, and sulfisoxazole, respectively. Recovery followed discontinuance of the drugs. An antiplatelet antibody requiring the presence of an appropriate drug for interaction with platelets was deomonstrated in each case by the 51Cr platelet lysis test using normal, paroxysmal nocturnal hemoglobinuric, or enzyme-treated normal platelets as target cells. These antibodies could not be detected by techniques that depend on clot retraction inhibition, complement fixation, or platelet factor-3 activation. Quinine-and quinidine-dependent antiplatelet antibodies in the serum of 16 patients who developed acute thrombocytopenia while taking either quinine or quinidine could be demonstrated readily with the 51Cr platelet lysis test and could also be detected by other methods employed.

Author List

Cimo PL, Pisciotta AV, Desai RG, Pino JL, Aster RH

MESH terms used to index this publication - Major topics in bold

Adolescent
Antibodies
Antibody Specificity
Blood Platelets
Chromium Radioisotopes
Diazepam
Female
Humans
Male
Middle Aged
Phenytoin
Quinidine
Quinine
Sulfisoxazole
Thrombocytopenia