Faculty Collaboration Database

Prenatal genotyping of Jk(a) and Jk(b) of the human Kidd blood group system by allele-specific polymerase chain reaction. Prenat Diagn 1998 Dec;18(12):1225-31

Date

01/13/1999

Pubmed ID

9885013

DOI

10.1002/(sici)1097-0223(199812)18:12<1225::aid-pd434>3.0.co;2-d

Scopus ID

2-s2.0-0032436376 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

An allele-specific polymerase chain reaction (ASPCR) assay for prenatal genotyping of the Kidd antigen system in order to identify pregnancies at risk for haemolytic disease of the newborn (HDN) was developed. Oligonucleotide primers were designed for ASPCR of JKA and JKB. A validation study was performed using DNA isolated from 54 serotyped whole blood samples and 8 amniocentesis samples. A concordance rate of 100 per cent was observed between serotyping and ASPCR detection of the JKA and JKB alleles. Experiments were conducted to quantify the maternal contamination that could be tolerated in Kidd ASPCR assays. The sensitivity of this assay ranged from 0.2 per cent when detecting the presence of JKB and JKA background, to 2 per cent for detecting the presence of JKA in a JKB background. This sensitive assay is particularly useful for rapid genotyping of fetal amniotic cells to identify pregnancies at risk for HDN due to incompatibilities within the Kidd blood group system.

Author List

Hessner MJ, Pircon RA, Johnson ST, Luhm RA

Author

Martin J. Hessner PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adult
Alleles
Amniocentesis
DNA
DNA Primers
Erythroblastosis, Fetal
Female
Genotype
Humans
Infant, Newborn
Kidd Blood-Group System
Polymerase Chain Reaction
Pregnancy
Pregnancy Complications, Hematologic
Prenatal Diagnosis
Reproducibility of Results
Serotyping