Insulin-like growth factor-II/mannose-6-phosphate receptor expression during early heart development. Dev Dyn 1996 Oct;207(2):195-203
Date
10/01/1996Pubmed ID
8906422DOI
10.1002/(SICI)1097-0177(199610)207:2<195::AID-AJA7>3.0.CO;2-CScopus ID
2-s2.0-0029853236 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
Expression of the insulin-like growth factor-II/mannose-6-phosphate (ICF-II/ M6P) receptor was examined during the major stages of heart morphogenesis in the chicken embryo. By using an affinity-purified antibody, Western blot analysis of total embryonic proteins from stages 5-24 revealed little if any IGF-II/M6P receptor protein until stage 7, approximately 8 hours prior to the appearance of the rudimentary myocardial tubes. Thereafter, receptor accumulation increased until stage 14, after which receptor protein levels remained constant, up to 7 days in ovo. Immunohistochemical localization revealed that, among all embryonic tissues at stages 10-24, the predominant site of receptor expression was the developing myocardium. Receptor expression was also immunohistochemically evaluated in a defined in vitro model of cardiogenesis in which explanted precardiac mesoderm is induced to undergo differentiation by co-explanted endoderm. In this system, as in vivo, IGF-II/M6P receptors were only detected after precardiac mesoderm had differentiated into a synchronously contractile multilayer which expressed cardiac alpha-actin. These findings indicate that the IGF-II/M6P receptor has an important role during early heart development.
Author List
McCormick KM, Dahms NM, Lough JAuthors
Nancy M. Dahms PhD Professor in the Biochemistry department at Medical College of WisconsinJohn W. Lough PhD Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsBlotting, Western
Chick Embryo
Fluorescent Antibody Technique, Indirect
Heart
Myocardium
Receptor, IGF Type 2
