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Gene targeting in the rat: advances and opportunities. Trends Genet 2010 Dec;26(12):510-8 PMID: 20869786 PMCID: PMC2991520

Pubmed ID

20869786

Abstract

The rat has long been a model favored by physiologists, pharmacologists and neuroscientists. However, over the past two decades, many investigators in these fields have turned to the mouse because of its gene modification technologies and extensive genomic resources. Although the genomic resources of the rat have nearly caught up, gene targeting has lagged far behind, limiting the value of the rat for many investigators. In the past two years, advances in transposon- and zinc finger nuclease (ZFN)-mediated gene knockout as well as the establishment and culturing of embryonic and inducible pluripotent stem cells have created new opportunities for rat genetic research. Here, we provide a high-level description and the potential uses of these new technologies for investigators using the rat for biomedical research.

Author List

Jacob HJ, Lazar J, Dwinell MR, Moreno C, Geurts AM

Authors

Melinda R. Dwinell PhD Center Associate Director, Associate Professor in the Physiology department at Medical College of Wisconsin
Aron Geurts PhD Associate Professor in the Physiology department at Medical College of Wisconsin




Scopus

2-s2.0-78549295495   76 Citations

MESH terms used to index this publication - Major topics in bold

Animals
Embryo, Mammalian
Embryonic Stem Cells
Gene Knockout Techniques
Gene Targeting
Humans
Mice
Rats
jenkins-FCD Prod-296 4db9d02597e0a2e889e230f853b641c12f1c3ee3