Endostatin induces endothelial cell apoptosis. J Biol Chem 1999 Apr 23;274(17):11721-6
Date
04/17/1999Pubmed ID
10206987DOI
10.1074/jbc.274.17.11721Scopus ID
2-s2.0-0033597130 (requires institutional sign-in at Scopus site) 592 CitationsAbstract
Endostatin, a carboxyl-terminal fragment of collagen XVIII, has been shown to regress tumors in mice. In this study, we have analyzed the mechanism of endostatin action on endothelial cells and nonendothelial cells. Endostatin treatment of cow pulmonary artery endothelial cells caused apoptosis, as demonstrated by three methods, annexin V-fluorescein isothiocyanate staining, caspase 3, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling assay. Moreover, addition of endostatin led to a marked reduction of the Bcl-2 and Bcl-XL anti-apoptotic protein, whereas Bax protein levels were unaffected. These effects were not seen in several nonendothelial cells. Collectively, these findings provide important mechanistic insight into endostatin action.
Author List
Dhanabal M, Ramchandran R, Waterman MJ, Lu H, Knebelmann B, Segal M, Sukhatme VPAuthor
Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsApoptosis
Biological Transport
Cattle
Cell Membrane
Cell Nucleus
Collagen
Collagen Type XVIII
Endostatins
Endothelium, Vascular
In Situ Nick-End Labeling
Mice
Peptide Fragments
Phosphatidylserines
