Nitric oxide synthase is increased following small intestinal transplantation in the rat. Neurosci Lett 1999 May 07;266(2):97-100
Date
06/03/1999Pubmed ID
10353336DOI
10.1016/s0304-3940(99)00270-0Scopus ID
2-s2.0-0033532246 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
Transplantation of the small intestine is a neural model that could include extrinsic denervation, loss of intrinsic enteric neurons, or loss of intrinsic neural pathways. Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase activity was measured in normal rat ileum, ileum 3 months after resection of the jejunum, and ileum 3 months after isotransplantation of the ileum. The distribution of NADPH diaphorase activity and immunoreactive neuronal nitric oxide synthase were examined. Nicotinamide adenine dinucleotide phosphate diaphorase activity was increased in transplanted ileum (16.5+/-3.5 mU/mg protein) compared to normal controls (6.6+/-0.7) and resection controls (6.8+/-0.6) (P < 0.05, ANOVA). Histologically, NADPH diaphorase activity and immunoreactive nitric oxide synthase appeared increased within nerve cell bodies following transplantation. These findings may represent an adaptive response of the enteric nervous system to extrinsic denervation. Loss of intrinsic neural pathways was not supported as a mechanism.
Author List
Stadelmann AM, Fink JG, Telford GL, Walgenbach-Telford S, Koch TRMESH terms used to index this publication - Major topics in bold
Analysis of VarianceAnimals
Intestine, Small
NADPH Dehydrogenase
Nitric Oxide Synthase
Rats
Rats, Inbred Lew