Medical College of Wisconsin
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Transurethral bladder tumor resection alters fibronectin expression in transitional carcinoma cell lines. J Urol 1997 Mar;157(3):1136-43 PMID: 9072558

Pubmed ID

9072558

Abstract

PURPOSE: To examine the effect of TGF beta 1 and post-transurethral resection of bladder tumor (TURBT) urine on fibronectin (FN) expression by transitional carcinoma (TCC) cell lines.

MATERIALS AND METHODS: Northern analysis and rtPCR for mRNA transcripts, in combination with ELISA and immunohistochemistry for protein, were used to quantify the effect of TGF beta 1 and post-TURBT urine on cellular expression of FN in two TCC lines, 253J and 647V. The TGF beta 1-dependent nature of the response seen in post-TURBT urine was confirmed using exogenous TGF beta 1 or blocking antibodies to TGF beta 1. Active and latent levels of TGFb1 were measured in post-TURBT urine and urine "spiked" cell culture media.

RESULTS: Baseline FN expression was low in the poorly differentiated, metastatic 253J line and high in the moderately differentiated 647V line. TGF beta 1 and post-TURBT urine increased FN expression at both the mRNA and protein levels in the 253J line. Anti-TGF beta 1 antibodies inhibited the effect of post-TURBT urine on FN expression in 253J cells. High levels of TGF beta 1 decreased FN expression in 647V cells. Only latent TGF beta 1 was present in samples of urine and media.

CONCLUSIONS: FN production in low expressing TCC lines can be augmented by exogenous TGF beta 1. TURBT increases latent urinary TGF beta 1 which in turn affects TCC FN expression. Altered tumor expression of FN following TURBT may influence the effectiveness of intravesical BCG.

Author List

See WA, Xu Y, Gee K, Severson C, Cohen MB, Ladehoff D

Author

William A. See MD Chair, Professor in the Urologic Surgery department at Medical College of Wisconsin




Scopus

2-s2.0-0031023234   7 Citations

MESH terms used to index this publication - Major topics in bold

Carcinoma, Transitional Cell
Fibronectins
Humans
Postoperative Period
RNA, Messenger
Transforming Growth Factor beta
Tumor Cells, Cultured
Urethra
Urinary Bladder Neoplasms
jenkins-FCD Prod-336 69ef4a6b262d135130251597d5d39873903802b5