Faculty Collaboration Database

Detection of adenovirus DNA in peripheral blood mononuclear cells by polymerase chain reaction assay. J Med Virol 1997 Mar;51(3):182-8

Date

03/01/1997

Pubmed ID

9139081

DOI

10.1002/(sici)1096-9071(199703)51:3<182::aid-jmv7>3.0.co;2-2

Scopus ID

2-s2.0-0030953127 (requires institutional sign-in at Scopus site)   48 Citations

Abstract

Adenovirus can establish persistent infections which may reactivate and cause disease in immunocompromised hosts. Lymphocytes have been postulated to serve as a site of adenoviral persistence based upon the ability to isolate adenovirus from tonsils and to detect adenovirus DNA by Southern blot hybridization in peripheral blood mononuclear cells (PBMC). To test this hypothesis, a more sensitive and specific polymerase chain reaction (PCR) assay was developed to detect adenovirus DNA. Two sets of nested primers were designed to conserved sequences in the adenovirus E1A and hexon genes. The E1A and hexon primers amplified DNA from representative adenoviral serotypes in all six adenoviral groups (A-F). Both primers detected a single copy of the adenovirus type 2 genome but were less sensitive for the group B type 35. None of 33 PBMC specimens from healthy adults and only one of 40 pediatric samples was positive (at a low level) for adenovirus DNA by nested PCR assay. In comparison, PBMC from two children with fatal adenoviral infection were both strongly positive for adenovirus DNA. It is concluded that, in contrast to a previous study, PBMC are not a common site of persistent group C adenoviral infection. In addition, assay of PBMC by the adenovirus-specific PCR may help detect early invasive disease and warrants further evaluation.

Author List

Flomenberg P, Gutierrez E, Piaskowski V, Casper JT

MESH terms used to index this publication - Major topics in bold

Adenoviridae Infections
Adenovirus E1A Proteins
Adenoviruses, Human
Adult
Antigens, Viral
Base Sequence
Capsid
Capsid Proteins
Child
Conserved Sequence
DNA Primers
DNA, Viral
Humans
Lymphocytes
Polymerase Chain Reaction
Reference Values
Sensitivity and Specificity
Serotyping