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The 75-kilodalton antigen of Bartonella bacilliformis is a structural homolog of the cell division protein FtsZ. J Bacteriol 1997 Jul;179(14):4545-52

Date

07/01/1997

Pubmed ID

9226264

Pubmed Central ID

PMC179290

DOI

10.1128/jb.179.14.4545-4552.1997

Abstract

A genomic library of Bartonella bacilliformis was constructed and screened with human anti-Bartonella serum from a patient with the chronic, verruga peruana phase of bartonellosis. An immunoreactive clone isolated from this library was found to code for a 591-amino-acid protein with a high degree of sequence similarity to the FtsZ family of proteins. The degree of amino acid identity between the B. bacilliformis protein (FtsZ[Bb]) and the other FtsZ proteins is especially pronounced over the N-terminal 321 amino acids (N-terminal domain) of the sequence, with values ranging from 45% identity for the homolog from Micrococcus luteus (FtsZ[Ml]) to 91% identity for the homolog from Rhizobium melliloti, (FtsZ[Rm1]). All of the functional domains required for FtsZ activity are conserved in FtsZ(Bb) and are located within the N-terminal domain of the protein. FtsZ(Bb) is approximately twice as large as most of the other FtsZ proteins previously reported, a property it shares with FtsZ(Rm1). Like the Rhizobium homolog, FtsZ(Bb) has a C-terminal region of approximately 256 amino acids that is absent in the other FtsZ proteins. Evidence is presented that implicates this region in the protein's antigenicity and suggests that, unlike most other FtsZ homologs, FtsZ(Bb) is at least partly exposed at the cell surface. PCR analysis revealed that an ftsZ gene similar in size to the B. bacilliformis gene is present in Bartonella henselae, a bacterium that is closely related to B. bacilliformis.

Author List

Padmalayam I, Anderson B, Kron M, Kelly T, Baumstark B

Author

Michael Kron MD Director, Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Amino Acid Sequence
Antigens, Bacterial
Bacterial Proteins
Bartonella
Bartonella Infections
Base Sequence
Blotting, Western
Cell Division
Cytoskeletal Proteins
GTP-Binding Proteins
Genes, Bacterial
Genomic Library
Guanosine Triphosphate
Humans
Immune Sera
Molecular Sequence Data
Molecular Weight
Polymerase Chain Reaction
Sequence Alignment
Sequence Analysis, DNA
Sinorhizobium meliloti
jenkins-FCD Prod-410 e9586552fe7f53c71f7923aa6e27aeabbd3c2473