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Surface biofilm disruption. Enhanced recovery of microorganisms from vascular prostheses. Arch Surg 1987 Jan;122(1):38-43

Date

01/01/1987

Pubmed ID

3541853

DOI

10.1001/archsurg.1987.01400130044006

Scopus ID

2-s2.0-0023089833   71 Citations

Abstract

Ultrasonic oscillation (sonication) of explanted vascular prosthetic graft material can disrupt surface biofilms and increase the recovery of adherent microorganisms. Recovery of microorganisms from vascular grafts was studied in a canine model of Staphylococcus epidermidis graft contamination (N = 26) and on graft material excised from patients undergoing femoral anastomotic pseudoaneurysm repair (N = 7). Surface biofilm disruption by sonication significantly increased the incidence of positive cultures of excised graft material compared with broth (P less than .010) and blood agar plate (P less than .005) culture techniques. The S epidermidis was recovered from 31% of the canine vascular grafts and 100% of the clinical specimens. The in vitro production of a glycocalyx "slime" was demonstrated in 73% of the recovered staphylococcal strains. The formation of an adherent bacteria biofilm on implanted vascular prostheses is not an uncommon occurrence and is an important factor in the pathogenesis of anastomotic pseudoaneurysm formation and late graft infection.

Author List

Tollefson DF, Bandyk DF, Kaebnick HW, Seabrook GR, Towne JB

Author

Gary R. Seabrook MD Chief, Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Bacteriological Techniques
Blood Vessel Prosthesis
Coronary Artery Bypass
Disease Models, Animal
Dogs
Equipment Contamination
Humans
Microscopy, Electron, Scanning
Polysaccharides, Bacterial
Sonication
Staphylococcal Infections
Staphylococcus epidermidis
jenkins-FCD Prod-398 336d56a365602aa89dcc112f077233607d6a5abc