Induction of apoptosis by (-)-gossypol-enriched cottonseed oil in human breast cancer cells. Int J Mol Med 2010 Jul;26(1):113-9
Date
06/02/2010Pubmed ID
20514430Scopus ID
2-s2.0-77954714438 (requires institutional sign-in at Scopus site) 22 CitationsAbstract
Induction of apoptosis is one of the mechanisms of chemotherapeutic agents against breast cancer. In addition, recent studies have shown that diets containing polyphenolic components possess anticancer activities either in vitro or in vivo by inhibiting cell proliferation and inducing apoptosis. The aim of our study was to explore the effects of (-)-gossypol-enriched cottonseed oil [(-)-GPCSO], a polyphenolic compound, on the proliferation of the breast cancer cell line MCF-7 as well as primary cultured human breast cancer epithelial cells (PCHBCEC). We investigated whether the mechanism of the effects of (-)-GPCSO was mediated via the induction of cell apoptosis and the regulation of Bcl-2 gene expression at both the mRNA and protein levels. Our results showed that (-)-GPCSO inhibited the proliferation of MCF-7 and PCHBCEC in a dose-dependent manner. (-)-GPCSO (0.1 and 0.2%) induced DNA fragmentation in both MCF-7 cells and PCHBCEC. (-)-GPCSO suppressed the expression of Bcl-2 at both the mRNA and protein levels in MCF-7 cells and PCHBCEC in a dose-dependent fashion. Our results suggest that the growth inhibitory effect of (-)-GPCSO on MCF-7 and PCHBCEC is due, at least partially, to the induction of cell apoptosis, which is mediated by down-regulation of Bcl-2 expression at both the mRNA and protein levels. It might be possible for (-)-GPCSO to be developed as a novel chemotherapeutic agent for breast cancer patients.
Author List
Ye W, Chang HL, Wang LS, Huang YW, Shu S, Sugimoto Y, Dowd MK, Wan PJ, Lin YCMESH terms used to index this publication - Major topics in bold
ApoptosisBlotting, Western
Breast Neoplasms
Cell Line, Tumor
Cell Proliferation
Cells, Cultured
Cottonseed Oil
DNA Fragmentation
Dose-Response Relationship, Drug
Electrophoresis, Agar Gel
Epithelial Cells
Female
Gossypol
Humans
Proto-Oncogene Proteins c-bcl-2
Reverse Transcriptase Polymerase Chain Reaction