A(1,2)BO(1,2) genotyping by multiplexed allele-specific PCR. Br J Haematol 1998 Jan;100(1):229-34
Date
02/05/1998Pubmed ID
9450817DOI
10.1046/j.1365-2141.1998.00535.xScopus ID
2-s2.0-0031972825 (requires institutional sign-in at Scopus site) 39 CitationsAbstract
The ABO blood group is the most clinically important human alloantigen system in transfusion medicine. The system involves three antigens A, B and H. H antigen is converted to either A or B by the activity of alpha1-->3-N-acetyl-galactosaminyl transferase (A transferase) or alpha1-->3 galactosyl transferase (B transferase). The O phenotype is the result of an inactive glycosyltransferase, which is unable to glycosylate the H antigen. The immunological properties of the ABO system were identified at the turn of the century; however, the genetic basis of the ABO system has only recently been characterized. This has enabled the development of a number of molecular ABO typing methods. Described here is a two-reaction multiplex allele-specific PCR (ASPCR) genotyping assay for the A1, A2, B, O1 and O2 subtypes. 11 different allele-specific oligonucleotide primers were selected to detect the presence or absence of the O1 associated G--> (-) deletion at base 261, the O2 associated G --> A substitution at base 802, the B associated G --> A substitution at base 803, and finally the A2 associated C --> (-) deletion at base 1059. A total of 122 peripheral blood samples were genotyped and serologically forward and reverse typed. A concordance rate of 98.4% (120/122 samples) was observed between the actual genotype and the serologically-based predicted genotype. These results indicate that this assay provides a rapid, accurate, and simple method for A(1,2)BO(1,2) genotyping that serves as a useful supplement to standard serological ABO typing.
Author List
Pearson SL, Hessner MJAuthor
Martin J. Hessner PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
ABO Blood-Group SystemAlleles
Base Sequence
Genotype
Humans
Molecular Sequence Data
Polymerase Chain Reaction