Iron attenuates nitric oxide level and iNOS expression in endotoxin-treated mice. FEBS Lett 1998 Mar 13;424(3):253-6
Date
04/16/1998Pubmed ID
9539161DOI
10.1016/s0014-5793(98)00181-1Scopus ID
2-s2.0-0032513035 (requires institutional sign-in at Scopus site) 22 CitationsAbstract
The effect of exogenous Fe-citrate complex (Fe doses of 120 and 240 micromol/kg) on nitric oxide (NO) production in vivo has been studied in blood and liver tissue of endotoxin-treated mice. Fe-citrate complex was administered to mice subcutaneously at the same time with intravenous injection of Escherichia coli lipopolysaccharide (LPS). Iron-dependent decrease in NO2-/NO3- and nitrosyl hemoglobin levels in blood of animals was detected at 6 h after LPS administration, suggesting systemic attenuation of NO generation. NO production in the liver tissue of LPS-treated mice was decreased after Fe administration judging from the amount of mononitrosyl-iron complexes formed in the tissue by diethyldithiocarbamate. The iNOS protein determination in the liver tissue of LPS-treated mice demonstrated iron-dependent inhibition of iNOS expression. We have found previously that exogenous iron does not affect systemic NO level when it is given at 6 h after LPS injection, i.e. after iNOS expression. This is a first report demonstrating iron-dependent iNOS down-regulation in endotoxin-treated mice.
Author List
Komarov AM, Mattson DL, Mak IT, Weglicki WBMESH terms used to index this publication - Major topics in bold
AnimalsCitric Acid
Ditiocarb
Electron Spin Resonance Spectroscopy
Endotoxins
Female
Iron
Liver
Mice
Mice, Inbred ICR
Nitric Oxide
Nitric Oxide Synthase
