Cartilage-derived morphogenetic protein-1 promotes the differentiation of mesenchymal stem cells into chondrocytes. Biochem Biophys Res Commun 2004 Dec 10;325(2):453-60
Date
11/09/2004Pubmed ID
15530414DOI
10.1016/j.bbrc.2004.10.055Scopus ID
2-s2.0-7744227932 (requires institutional sign-in at Scopus site) 84 CitationsAbstract
Mesenchymal stem cells (MSCs) are able to differentiate into many types of cells including chondrocytes. Transforming growth factor beta1 (TGF-beta1) is very important in the regulation of chondrogenesis. Since cartilage-derived morphogenetic protein-1 (CDMP-1) belongs to the TGF-beta superfamily, we tested whether CDMP-1 plays any role in the regulation of the differentiation of MSCs into chondrocytes using a high density pellet culture system. Based on the histological staining of glycosaminoglycan using toluidine blue dye-binding method we found that CDMP-1 could initiate chondrogenic differentiation of MSCs as did TGF-beta1. However, CDMP-1 was less stimulatory than TGF-beta1. The combination of CDMP-1 and TGF-beta1 synergically induced chondrogenesis of MSCs. This synergic chondrogenic effect of CDMP-1 together with TGF-beta1 was further confirmed by quantification of GAG using dimethylmethylene blue dye-binding assay and immunohistochemical analysis of the expression of cartilage-specific protein collagen II. This study may provide an improved induction approach using MSCs for repairing damaged cartilage.
Author List
Bai X, Xiao Z, Pan Y, Hu J, Pohl J, Wen J, Li LAuthor
Xiaowen Bai PhD Associate Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Aborted FetusBone Morphogenetic Proteins
Cell Differentiation
Cell Proliferation
Chondrocytes
Chondrogenesis
Collagen
Dose-Response Relationship, Drug
Gene Expression
Glycosaminoglycans
Growth Differentiation Factor 5
Humans
Transforming Growth Factor beta
Transforming Growth Factor beta1
