Human COX6A1 gene: promoter analysis, cDNA isolation and expression in the monkey brain. Gene 2000 Apr 18;247(1-2):63-75
Date
04/25/2000Pubmed ID
10773445DOI
10.1016/s0378-1119(00)00121-9Scopus ID
2-s2.0-0034682036 (requires institutional sign-in at Scopus site) 25 CitationsAbstract
The human COX6A1 gene encodes the ubiquitous isoform of cytochrome c oxidase (COX) subunit VIa (VIa-L), and is located in a CpG island on chromosome 12q24.2. We compared the COX6A1 gene with the published cDNA and several ESTs and concluded that subunit COX VIa-L is synthesized as a preprotein, as are other COX subunits. The same transcription start sites were identified by primer extension analysis of human brain and lymphoblastoid RNA. Analysis of the COX6A1 promoter revealed several conserved sequence elements found in other COX genes, namely binding sites for nuclear respiratory factor 1 (NRF-1), nuclear respiratory factor 2/GA binding protein (NRF-2/GABP), and ying-yang protein 1 (YY1). These conserved elements were shown to bind nuclear proteins from HeLa nuclear extracts. COX6A1 cDNA was isolated from a human brain cDNA library, and the sequence was identical to that of human liver. The expression of this gene was demonstrated by in-situ hybridization in monkey brain sections with our human brain cDNA. Monocular impulse blockade in adult monkeys induced a downregulation of COX6A1 expression in deprived visual neurons, suggesting that this subunit gene is regulated by neuronal activity.
Author List
Wong-Riley M, Guo A, Bachman NJ, Lomax MIMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Base Sequence
Binding Sites
Brain
DNA, Complementary
DNA-Binding Proteins
Electron Transport Complex IV
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Enzymologic
Genes
Geniculate Bodies
HeLa Cells
Humans
In Situ Hybridization
Isoenzymes
Macaca
Molecular Sequence Data
Oligonucleotides
Promoter Regions, Genetic
RNA, Messenger
Rats
Regulatory Sequences, Nucleic Acid
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tetrodotoxin
Transcription, Genetic
