Erythroid phosphatidyl serine exposure is not predictive of thrombotic risk in mice with hemolytic anemia. Blood Cells Mol Dis 2000 Feb;26(1):75-83
Date
04/25/2000Pubmed ID
10772878DOI
10.1006/bcmd.2000.0277Scopus ID
2-s2.0-0034144577 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
Thrombosis is a major complication of human hemolytic anemias such as sickle cell disease, thalassemia, and severe hereditary spherocytosis (HS). Mice with severe HS and severe hereditary elliptocytosis (HE) also suffer from thrombosis, with incidences ranging from 15 and 22% in beta-spectrin- and ankyrin-deficient mice, respectively, to 85 to 100% in alpha-spectrin-deficient and band 3 knockout mice. A contributing factor to thrombosis could be loss of phospholipid asymmetry of the mutant red blood cells (RBCs), with concomitant exposure of the aminophospholipid phosphatidylserine (PS). Increased PS exposure occurs in RBCs from sickle cell and thalassemia patients and in RBCs from band 3-deficient mice. To determine if increased PS exposure correlates with thrombotic risk in HS and HE mice with ankyrin, beta-spectrin, and alpha-spectrin deficiencies, measurements of FITC-labeled annexin V binding to externalized PS on RBCs were performed. PS exposure is elevated in all mice with HS and HE, but the percentage of RBCs with exposed PS does not correlate with thrombotic risk in these mice.
Author List
Wandersee NJ, Tait JF, Barker JEMESH terms used to index this publication - Major topics in bold
Age FactorsAnemia, Hemolytic
Animals
Animals, Newborn
Annexin A5
Cell Size
Erythrocyte Membrane
Erythrocytes
Flow Cytometry
Fluorescein-5-isothiocyanate
Lipid Bilayers
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Phosphatidylserines
Spectrin
Thrombosis
