Sequence-based typing of HLA class II DQB1. Tissue Antigens 2000 Apr;55(4):364-8
Date
06/14/2000Pubmed ID
10852389DOI
10.1034/j.1399-0039.2000.550411.xScopus ID
2-s2.0-0034013698 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
Due to the expanding number of known HLA class II DQB1 alleles, high-resolution oligotyping is becoming ineffective, therefore a sequence-based typing (SBT) strategy was developed to provide rapid and definitive typing of HLA-DQB1. HLA-DQB1*02, *03, *04, *05, and *06 alleles were individually amplified by polymerase chain reaction (PCR) using exon 2 group-specific primers. Forward and reverse PCR primers were tailed with M13 universal and M13 reverse sequences, respectively. Subsequent bi-directional cycle-sequencing was carried out using Cy5.5-labeled M13 universal primer and Cy5.0-labeled M13 reverse primer. Automated sequencing was performed in 30 min using a Visible Genetics, Inc. (VGI) MicroGene Clipper Sequencer. Full concordance was observed between this SBT method and oligotyping among 151 individuals.
Author List
Dinauer DM, Luhm RA, Uzgiris AJ, Eckels DD, Hessner MJAuthor
Martin J. Hessner PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
DNA PrimersExons
Genotype
HLA-DQ Antigens
HLA-DQ beta-Chains
Histocompatibility Testing
Humans
Polymerase Chain Reaction
Sequence Analysis, DNA