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Endothelial microparticles induce inflammation in acute lung injury. J Surg Res 2011 Mar;166(1):32-9 PMID: 20828748 PMCID: PMC4731030

Pubmed ID

20828748

DOI

10.1016/j.jss.2010.05.036

Abstract

BACKGROUND: Previously, we have shown that endothelial microparticles (EMPs) injected into mice induce acute lung injury (ALI) [1]. In this study, we hypothesize that EMPs induce ALI by initiating cytokine release in the lung, leading to recruitment and activation of neutrophils.

MATERIALS AND METHODS: C57BL/6J male mice (8-10 wk old) were intravenously injected with EMPs (200,000/mL), LPS (2 mg/kg), or both. Bronchoalveolar lavage (BAL) and serum levels of IL-1β and TNF-α were analyzed by enzyme-linked immunoassay (ELISA). Morphometric analysis was performed on H and E stained lung sections. Myeloperoxidase (MPO) levels were determined via an enzymatic assay and immunofluorescence of stained sections.

RESULTS: EMPs led to significantly increased pulmonary and systemic IL-1β and TNF-α levels, which correlated with increased neutrophil recruitment to the lung. MPO levels in the lungs were increased significantly following injection of EMPs or LPS, compared to PBS. In mice treated with EMPs and LPS either simultaneously or successively, the cytokine and MPO levels were significantly increased over that of either treatment alone.

CONCLUSION: EMPs contribute to lung injury through the initiation of a cytokine cascade that increases recruitment of neutrophils and subsequent release of MPO. Furthermore, treatment of mice with both EMPs and LPS induced greater lung injury than either treatment alone, suggesting that EMPs prime the lung for increased injury by other pathogens. Therapies aimed at reducing or blocking EMPs may be a useful strategy for attenuating lung injury.

Author List

Buesing KL, Densmore JC, Kaul S, Pritchard KA Jr, Jarzembowski JA, Gourlay DM, Oldham KT

Authors

John C. Densmore MD Associate Professor in the Surgery department at Medical College of Wisconsin
David M. Gourlay MD Chief, Professor in the Surgery department at Medical College of Wisconsin
Jason A. Jarzembowski MD, PhD Vice Chair, Associate Professor in the Pathology department at Medical College of Wisconsin
Keith T. Oldham MD Professor in the Surgery department at Medical College of Wisconsin
Kirkwood A. Pritchard PhD Professor in the Surgery department at Medical College of Wisconsin




Scopus

2-s2.0-79951678163   35 Citations

MESH terms used to index this publication - Major topics in bold

Acute Lung Injury
Animals
Bronchoalveolar Lavage Fluid
Cell-Derived Microparticles
Endothelial Cells
Humans
Interleukin-1beta
Male
Mice
Mice, Inbred C57BL
Neutrophils
Peroxidase
Pneumonia
Tumor Necrosis Factor-alpha
Umbilical Veins
jenkins-FCD Prod-336 69ef4a6b262d135130251597d5d39873903802b5