Conformational and linear B-cell epitopes of Asp f 2, a major allergen of Aspergillus fumigatus, bind differently to immunoglobulin E antibody in the sera of allergic bronchopulmonary aspergillosis patients. Infect Immun 1999 May;67(5):2284-91
Date
05/04/1999Pubmed ID
10225885Pubmed Central ID
PMC115968DOI
10.1128/IAI.67.5.2284-2291.1999Scopus ID
2-s2.0-0032901306 (requires institutional sign-in at Scopus site) 50 CitationsAbstract
Asp f 2 is a major Aspergillus fumigatus allergen involved in allergic bronchopulmonary aspergillosis. Knowledge of the B-cell epitopes may contribute to the understanding of immunoregulation and immunodiagnosis. To elucidate the immunoglobulin E (IgE) binding epitopes in the linear sequence of Asp f 2, we synthesized decamer peptides spanning the whole molecule of Asp f 2 on derivatized cellulose membranes and evaluated IgE binding in ABPA patient and control sera. Peptides three to five amino acids long were synthesized based on amino acid sequences within the IgE binding regions and evaluated for the specificity of epitope antibody interactions. Nine IgE binding regions were recognized in this protein of 268 amino acid residues. Of the nine epitopes, seven (ATQRRQI, RKYFG, HWR, YTTRR, DHFAD, ALEAYA, and THEGGQ) are present in the hydrophilic regions of Asp f 2. Immunologic evaluation of the three recombinant fragments, Asp f 2A encompassing the N-terminal epitope region, Asp f 2B without N- and C-terminal regions of the protein, and Asp f 2C representing C-terminal epitopes, revealed that either the N- or C-terminal region of the protein is essential for the correct folding and conformation for IgE antibody binding.
Author List
Banerjee B, Greenberger PA, Fink JN, Kurup VPAuthor
Banani Banerjee PhD Associate Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AllergensAmino Acid Sequence
Antibodies, Fungal
Antibody Specificity
Antigens, Fungal
Aspergillosis, Allergic Bronchopulmonary
Aspergillus fumigatus
B-Lymphocytes
Base Sequence
Case-Control Studies
DNA Primers
Epitopes
Fungal Proteins
Humans
Immunoglobulin E
Molecular Sequence Data
Protein Conformation
