Cutting edge: the tyrosine phosphatase SHP-1 regulates thymocyte positive selection. J Immunol 1999 May 15;162(10):5680-4
Date
05/07/1999Pubmed ID
10229799Scopus ID
2-s2.0-0033562992 (requires institutional sign-in at Scopus site) 46 CitationsAbstract
The binding kinetics of the TCR for its interacting ligand and the nature of the resulting signal transduction event determine the fate of a developing thymocyte. The intracellular tyrosine phosphatase SHP-1 is a potential regulator of the TCR signal transduction cascade and may affect thymocyte development. To assess the role of SHP-1 in thymocyte development, we generated T cell-transgenic mice that express a putative dominant negative form of SHP-1, in which a critical cysteine is mutated to serine (SHP-1 C453S). SHP-1 C453S mice that express the 3.L2 TCR transgene are increased in CD4 single positive cells in the thymus and are increased in cells that express the clonotypic TCR. These data suggest that the expression of SHP-1 C453S results in increased positive selection in 3.L2 TCR-transgenic mice and support a role for SHP-1 thymocyte development.
Author List
Plas DR, Williams CB, Kersh GJ, White LS, White JM, Paust S, Ulyanova T, Allen PM, Thomas MLAuthor
Calvin B. Williams MD, PhD Chief, Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCD4-Positive T-Lymphocytes
Cysteine
Intracellular Signaling Peptides and Proteins
Mice
Mice, Transgenic
Mutation
Protein Tyrosine Phosphatase, Non-Receptor Type 11
Protein Tyrosine Phosphatase, Non-Receptor Type 6
Protein Tyrosine Phosphatases
Receptors, Antigen, T-Cell
SH2 Domain-Containing Protein Tyrosine Phosphatases
Serine
Signal Transduction
Spleen
Thymus Gland
src Homology Domains